WO2002008766A1 - Probe for analyzing protein-protein interaction and method of analyzing protein-protein interactions with the use of the same - Google Patents

Probe for analyzing protein-protein interaction and method of analyzing protein-protein interactions with the use of the same Download PDF

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Publication number
WO2002008766A1
WO2002008766A1 PCT/JP2000/009348 JP0009348W WO0208766A1 WO 2002008766 A1 WO2002008766 A1 WO 2002008766A1 JP 0009348 W JP0009348 W JP 0009348W WO 0208766 A1 WO0208766 A1 WO 0208766A1
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WO
WIPO (PCT)
Prior art keywords
protein
analyzing
probe
interaction
interactions
Prior art date
Application number
PCT/JP2000/009348
Other languages
French (fr)
Japanese (ja)
Inventor
Yoshio Umezawa
Takeaki Ozawa
Original Assignee
Japan Science And Technology Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science And Technology Corporation filed Critical Japan Science And Technology Corporation
Priority to JP2002514409A priority Critical patent/JP3772193B2/en
Priority to US10/089,040 priority patent/US7166447B2/en
Priority to EP00987766A priority patent/EP1229330B1/en
Priority to DE60033551T priority patent/DE60033551T2/en
Priority to CA002385830A priority patent/CA2385830C/en
Publication of WO2002008766A1 publication Critical patent/WO2002008766A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

To enable the provision of a probe for analyzing a protein-protein interaction, whereby protein-protein interactions of among any proteins can be highly accurately and conveniently analyzed, and the analysis of a protein-protein interaction with the use of this probe, a probe for analyzing an interaction between two proteins, whereby a protein splicing is induced by the protein-protein interaction and a physicochemically or biochemically detectable protein is thus regenerated, is provided.
PCT/JP2000/009348 2000-07-26 2000-12-27 Probe for analyzing protein-protein interaction and method of analyzing protein-protein interactions with the use of the same WO2002008766A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2002514409A JP3772193B2 (en) 2000-07-26 2000-12-27 Protein-protein interaction analysis probe and method for analyzing protein-protein interaction using the same
US10/089,040 US7166447B2 (en) 2000-07-26 2000-12-27 Probe for analyzing protein—protein interaction and method of analyzing protein—protein interactions with the use of the same
EP00987766A EP1229330B1 (en) 2000-07-26 2000-12-27 Probe for analyzing protein-protein interaction and method of analyzing protein-protein interactions with the use of the same
DE60033551T DE60033551T2 (en) 2000-07-26 2000-12-27 PROBE FOR ANALYZING PROTEIN-PROTEIN INTERACTIONS AND CORRESPONDING METHOD THEREFOR
CA002385830A CA2385830C (en) 2000-07-26 2000-12-27 Probe for protein-protein interaction analysis, and method of using it for analysis of protein-protein interaction

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000/224939 2000-07-26
JP2000224939 2000-07-26

Publications (1)

Publication Number Publication Date
WO2002008766A1 true WO2002008766A1 (en) 2002-01-31

Family

ID=18718794

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2000/009348 WO2002008766A1 (en) 2000-07-26 2000-12-27 Probe for analyzing protein-protein interaction and method of analyzing protein-protein interactions with the use of the same

Country Status (6)

Country Link
US (1) US7166447B2 (en)
EP (1) EP1229330B1 (en)
JP (1) JP3772193B2 (en)
CA (1) CA2385830C (en)
DE (1) DE60033551T2 (en)
WO (1) WO2002008766A1 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005078119A1 (en) * 2004-02-12 2005-08-25 Japan Science And Technology Agency Probe for detecting nuclear receptor agonist or antagonist and method of screening agonist or antagonist to nuclear receptor with the use of the same
US7855167B2 (en) * 1998-02-02 2010-12-21 Odyssey Thera, Inc. In vivo screening of protein-protein interactions with protein-fragment complementation assays
US8183036B2 (en) 2003-10-10 2012-05-22 Promega Corporation Luciferase biosensor
US8735559B2 (en) 2010-05-11 2014-05-27 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US9045730B2 (en) 2008-05-19 2015-06-02 Promega Corporation Luciferase biosensors for cAMP
US9290794B2 (en) 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US9359635B2 (en) 2006-04-03 2016-06-07 Promega Corporation Permuted and nonpermuted luciferase biosensors

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003089464A1 (en) * 2002-04-19 2003-10-30 Bioimage A/S Two green fluorescent protein fragments and their use in a method for detecting protein - protein interactions
JP4287633B2 (en) * 2002-09-18 2009-07-01 独立行政法人科学技術振興機構 Analyzing method and materials for organelle localization protein
CA2526650A1 (en) * 2003-05-22 2004-12-02 Japan Science And Technology Agency A pair of probes for analyzing protein-protein interaction and a method for analyzing protein-protein interaction using the same
WO2005118790A2 (en) * 2004-06-03 2005-12-15 The Trustees Of Columbia University In The City Of New York Combinatorial marking of cells and cell structures with reconstituted fluorescent proteins
US20070026428A1 (en) * 2005-05-02 2007-02-01 Chelur Dattananda S Combinatorial expression of split caspase molecules
EP1811031A1 (en) 2006-01-18 2007-07-25 Millegen Method for selecting a peptide or polypeptide which binds to a target molecule
JP4491516B2 (en) 2007-05-09 2010-06-30 国立大学法人 東京大学 Activated protease indicator
JP5816412B2 (en) * 2010-03-25 2015-11-18 東洋ビーネット株式会社 Target protein detection method using split reporter protein
CN101975776B (en) * 2010-10-22 2012-06-27 上海交通大学 Genetic engineering kit for detecting environmental estrogen pollutants and application thereof
KR102443088B1 (en) * 2016-07-27 2022-09-14 얼라인 테크널러지, 인크. Intraoral scanner with dental diagnostics

Citations (4)

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US5795731A (en) * 1996-08-26 1998-08-18 Health Research Incorporated Inteins as antimicrobial targets: genetic screens for intein function
WO2000018881A2 (en) * 1998-09-30 2000-04-06 New England Biolabs, Inc. Intein mediated peptide ligation
WO2000034514A1 (en) * 1998-12-08 2000-06-15 Proteus (S.A.) METHOD FOR DETERMINING THE ACTIVITY OF A SUBSTANCE USING A FUNCTIONAL TEST $i(IN VITRO)
WO2000036093A2 (en) * 1998-12-18 2000-06-22 The Penn State Research Foundation Intein-mediated cyclization of peptides

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US5834247A (en) * 1992-12-09 1998-11-10 New England Biolabs, Inc. Modified proteins comprising controllable intervening protein sequences or their elements methods of producing same and methods for purification of a target protein comprised by a modified protein
DE69333667T2 (en) * 1992-12-09 2006-02-02 New England Biolabs, Inc., Beverly Modified proteins containing controllable intermediate sequences and methods for their preparation
US6770446B1 (en) * 1994-06-14 2004-08-03 Wyeth Cell systems having specific interaction of peptide binding pairs
US5998136A (en) * 1996-08-19 1999-12-07 Arcaris, Inc. Selection systems and methods for identifying genes and gene products involved in cell proliferation
US6875594B2 (en) * 1997-11-13 2005-04-05 The Rockefeller University Methods of ligating expressed proteins
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Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
US5795731A (en) * 1996-08-26 1998-08-18 Health Research Incorporated Inteins as antimicrobial targets: genetic screens for intein function
WO2000018881A2 (en) * 1998-09-30 2000-04-06 New England Biolabs, Inc. Intein mediated peptide ligation
WO2000034514A1 (en) * 1998-12-08 2000-06-15 Proteus (S.A.) METHOD FOR DETERMINING THE ACTIVITY OF A SUBSTANCE USING A FUNCTIONAL TEST $i(IN VITRO)
WO2000036093A2 (en) * 1998-12-18 2000-06-22 The Penn State Research Foundation Intein-mediated cyclization of peptides

Non-Patent Citations (2)

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Title
M.J. CLOSTON ET AL.: "The ins and outs of protein splicing elements", MOLECULAR MICROBIOLOGY, vol. 12, no. 3, 1994, pages 359 - 363, XP002937473 *
See also references of EP1229330A4 *

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7855167B2 (en) * 1998-02-02 2010-12-21 Odyssey Thera, Inc. In vivo screening of protein-protein interactions with protein-fragment complementation assays
US8183036B2 (en) 2003-10-10 2012-05-22 Promega Corporation Luciferase biosensor
US8673558B2 (en) 2003-10-10 2014-03-18 Promega Corporation Luciferase biosensor
US9290745B2 (en) 2003-10-10 2016-03-22 Promega Corporation Luciferase biosensor
WO2005078119A1 (en) * 2004-02-12 2005-08-25 Japan Science And Technology Agency Probe for detecting nuclear receptor agonist or antagonist and method of screening agonist or antagonist to nuclear receptor with the use of the same
US9359635B2 (en) 2006-04-03 2016-06-07 Promega Corporation Permuted and nonpermuted luciferase biosensors
US10077433B2 (en) 2006-04-03 2018-09-18 Promega Corporation Permuted and nonpermuted luciferase biosensors
US9045730B2 (en) 2008-05-19 2015-06-02 Promega Corporation Luciferase biosensors for cAMP
US9879306B2 (en) 2008-05-19 2018-01-30 Promega Corporation Luciferase biosensors for cAMP
US9248201B2 (en) 2010-05-11 2016-02-02 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US9339561B2 (en) 2010-05-11 2016-05-17 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US9757478B2 (en) 2010-05-11 2017-09-12 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US9290794B2 (en) 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US8735559B2 (en) 2010-05-11 2014-05-27 Promega Corporation Mutant protease biosensors with enhanced detection characteristics

Also Published As

Publication number Publication date
DE60033551T2 (en) 2007-11-08
JP3772193B2 (en) 2006-05-10
CA2385830A1 (en) 2002-01-31
EP1229330B1 (en) 2007-02-21
DE60033551D1 (en) 2007-04-05
EP1229330A4 (en) 2002-11-04
EP1229330A1 (en) 2002-08-07
US20030003506A1 (en) 2003-01-02
US7166447B2 (en) 2007-01-23
CA2385830C (en) 2005-12-20

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