WO2002006445A3 - Transgenic mice containing targeted gene disruptions - Google Patents

Transgenic mice containing targeted gene disruptions Download PDF

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Publication number
WO2002006445A3
WO2002006445A3 PCT/US2001/022402 US0122402W WO0206445A3 WO 2002006445 A3 WO2002006445 A3 WO 2002006445A3 US 0122402 W US0122402 W US 0122402W WO 0206445 A3 WO0206445 A3 WO 0206445A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
present
transgenic mice
function
transgenic animals
Prior art date
Application number
PCT/US2001/022402
Other languages
French (fr)
Other versions
WO2002006445A2 (en
WO2002006445A9 (en
Inventor
Thomas J Brennan
Keith D Allen
Original Assignee
Deltagen Inc
Thomas J Brennan
Keith D Allen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deltagen Inc, Thomas J Brennan, Keith D Allen filed Critical Deltagen Inc
Priority to AU2001282894A priority Critical patent/AU2001282894A1/en
Publication of WO2002006445A2 publication Critical patent/WO2002006445A2/en
Publication of WO2002006445A9 publication Critical patent/WO2002006445A9/en
Publication of WO2002006445A3 publication Critical patent/WO2002006445A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knockout animals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Abstract

The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in a target gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene expression and gene function, and as potential treatments for various disease states and disease conditions. The transgenic animals of the present invention are defective in the function of a cysteine protease-like gene, a platelet activating factor receptor gene, a phenol sulfotransferase gene or an orphan G protein-coupled receptor /GPR-22) gene. In another aspect, the transgenic animals of the present invention compromise a phenotype associated with having a mutation in a cysteine protease-like gene, a platelet activating factor receptor gene, phonol sulfotransferase gene or an orphan G potein-coupled receptor (GPR-22) gene.
PCT/US2001/022402 2000-07-19 2001-07-17 Transgenic mice containing targeted gene disruptions WO2002006445A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001282894A AU2001282894A1 (en) 2000-07-19 2001-07-17 Transgenic mice containing targeted gene disruptions

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US21916800P 2000-07-19 2000-07-19
US21917100P 2000-07-19 2000-07-19
US21917500P 2000-07-19 2000-07-19
US60/219,171 2000-07-19
US60/219,168 2000-07-19
US60/219,175 2000-07-19
US22145500P 2000-07-25 2000-07-25
US60/221,455 2000-07-25
US25621200P 2000-12-13 2000-12-13
US60/256,212 2000-12-13

Publications (3)

Publication Number Publication Date
WO2002006445A2 WO2002006445A2 (en) 2002-01-24
WO2002006445A9 WO2002006445A9 (en) 2003-04-03
WO2002006445A3 true WO2002006445A3 (en) 2003-08-21

Family

ID=27539785

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/022402 WO2002006445A2 (en) 2000-07-19 2001-07-17 Transgenic mice containing targeted gene disruptions

Country Status (2)

Country Link
AU (1) AU2001282894A1 (en)
WO (1) WO2002006445A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002061432A2 (en) * 2001-02-01 2002-08-08 Lifespan Biosciences, Inc. Gpr22, a g protein-coupled receptor (gpcr) and compositions and methods related thereto
US7611832B2 (en) 2002-08-01 2009-11-03 Arena Pharmaceuticals, Inc. Human G protein-coupled receptor and modulators thereof for the treatment of ischemic heart disease and congestive heart failure
US8055347B2 (en) 2005-08-19 2011-11-08 Brainsgate Ltd. Stimulation for treating brain events and other conditions
US9233245B2 (en) 2004-02-20 2016-01-12 Brainsgate Ltd. SPG stimulation
US8010189B2 (en) 2004-02-20 2011-08-30 Brainsgate Ltd. SPG stimulation for treating complications of subarachnoid hemorrhage
US9675796B2 (en) 2013-11-10 2017-06-13 Brainsgate Ltd. Implant and delivery system for neural stimulator
US10271907B2 (en) 2015-05-13 2019-04-30 Brainsgate Ltd. Implant and delivery system for neural stimulator

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6077990A (en) * 1997-08-12 2000-06-20 Ortho-Mcneil Pharmaceutical, Inc. PAR2 modified transgenic mice

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6077990A (en) * 1997-08-12 2000-06-20 Ortho-Mcneil Pharmaceutical, Inc. PAR2 modified transgenic mice

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CAPECCHI M R: "TARGETED GENE REPLACEMENT", SCIENTIFIC AMERICAN, SCIENTIFIC AMERICAN INC. NEW YORK, US, March 1994 (1994-03-01), pages 52 - 59, XP002940139, ISSN: 0036-8733 *
DATABASE EMBL [online] 27 April 2000 (2000-04-27), "AW742498 AW742498.1 up58a08.y1 Soares_mouse_NMI", XP002222700, Database accession no. AW742498 *
MANSOUR S L ET AL: "DISRUPTION OF THE PROTO-ONCOGENE INT-2 IN MOUSE EMBRYO-DERIVED STEM CELLS: A GENERAL STRATEGY FOR TARGETING MUTATIONS TO NON-SELECTABLE GENES", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 336, 24 November 1988 (1988-11-24), pages 348 - 352, XP002052220, ISSN: 0028-0836 *

Also Published As

Publication number Publication date
WO2002006445A2 (en) 2002-01-24
WO2002006445A9 (en) 2003-04-03
AU2001282894A1 (en) 2002-01-30

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