WO2002000716A3 - Cell lines and cell-based assays for identification of androgen receptor modulators - Google Patents

Cell lines and cell-based assays for identification of androgen receptor modulators Download PDF

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Publication number
WO2002000716A3
WO2002000716A3 PCT/US2001/019609 US0119609W WO0200716A3 WO 2002000716 A3 WO2002000716 A3 WO 2002000716A3 US 0119609 W US0119609 W US 0119609W WO 0200716 A3 WO0200716 A3 WO 0200716A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
androgen receptor
receptor modulators
identification
based assays
Prior art date
Application number
PCT/US2001/019609
Other languages
French (fr)
Other versions
WO2002000716A2 (en
Inventor
Jacek Ostrowski
Joyce Driscoll
John Lupisella
Mark Salvati
Original Assignee
Bristol Myers Squibb Co
Jacek Ostrowski
Joyce Driscoll
John Lupisella
Mark Salvati
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co, Jacek Ostrowski, Joyce Driscoll, John Lupisella, Mark Salvati filed Critical Bristol Myers Squibb Co
Priority to MXPA02012573A priority Critical patent/MXPA02012573A/en
Priority to EP01944616A priority patent/EP1297108A2/en
Priority to IL15249101A priority patent/IL152491A0/en
Priority to CA002413596A priority patent/CA2413596A1/en
Priority to JP2002505838A priority patent/JP2004517606A/en
Priority to AU2001267008A priority patent/AU2001267008A1/en
Priority to PCT/US2001/019609 priority patent/WO2002000716A2/en
Publication of WO2002000716A2 publication Critical patent/WO2002000716A2/en
Publication of WO2002000716A3 publication Critical patent/WO2002000716A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • A61K31/407—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with other heterocyclic ring systems, e.g. ketorolac, physostigmine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/08—Bridged systems

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Stable muscle cell lines comprising an androgen receptor and methods of using these cells in functional transactivation assays to assess the efficacy of compounds as androgen receptor modulators in a muscle cell background are provided.
PCT/US2001/019609 2000-06-28 2001-06-20 Cell lines and cell-based assays for identification of androgen receptor modulators WO2002000716A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
MXPA02012573A MXPA02012573A (en) 2000-06-28 2001-06-20 Cell lines and cell-based assays for identification of androgen receptor modulators.
EP01944616A EP1297108A2 (en) 2000-06-28 2001-06-20 Cell lines and cell-based assays for identification of androgen receptor modulators
IL15249101A IL152491A0 (en) 2000-06-28 2001-06-20 Stable muscle cell lines and cell-based assay methods for identification of androgen receptor modulations
CA002413596A CA2413596A1 (en) 2000-06-28 2001-06-20 Cell lines and cell-based assays for identification of androgen receptor modulators
JP2002505838A JP2004517606A (en) 2000-06-28 2001-06-20 Cell lines and cell-based assays for identification of androgen receptor modulators
AU2001267008A AU2001267008A1 (en) 2000-06-28 2001-06-20 Cell lines and cell-based assays for identification of androgen receptor modulators
PCT/US2001/019609 WO2002000716A2 (en) 2000-06-28 2001-06-20 Cell lines and cell-based assays for identification of androgen receptor modulators

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21439200P 2000-06-28 2000-06-28
US60/214,392 2000-06-28
PCT/US2001/019609 WO2002000716A2 (en) 2000-06-28 2001-06-20 Cell lines and cell-based assays for identification of androgen receptor modulators

Publications (2)

Publication Number Publication Date
WO2002000716A2 WO2002000716A2 (en) 2002-01-03
WO2002000716A3 true WO2002000716A3 (en) 2002-06-13

Family

ID=26680504

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/019609 WO2002000716A2 (en) 2000-06-28 2001-06-20 Cell lines and cell-based assays for identification of androgen receptor modulators

Country Status (6)

Country Link
EP (1) EP1297108A2 (en)
JP (1) JP2004517606A (en)
AU (1) AU2001267008A1 (en)
CA (1) CA2413596A1 (en)
MX (1) MXPA02012573A (en)
WO (1) WO2002000716A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1359160A1 (en) * 2002-02-28 2003-11-05 Pfizer Products Inc. Androgen receptor overexpressing skeletal myoblasts
WO2004007753A2 (en) * 2002-07-17 2004-01-22 Bristol-Myers Squibb Company Transgenic non-human mammals expressing a reporter nucleic acid under the regulation of androgen response elements
WO2009136629A1 (en) * 2008-05-09 2009-11-12 あすか製薬株式会社 Prophylactic/therapeutic agent for lifestyle-related diseases
AR092982A1 (en) 2012-10-11 2015-05-13 Isis Pharmaceuticals Inc MODULATION OF THE EXPRESSION OF ANDROGEN RECEIVERS
CN105758916B (en) * 2016-03-16 2018-04-13 济南大学 A kind of preparation method and application of the decis optical electro-chemistry sensor based on electrochemical luminescence excitation
CN115181748A (en) * 2022-08-08 2022-10-14 湖州师范学院 Biological detection method of male hormone

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988003168A1 (en) * 1986-10-24 1988-05-05 The Salk Institute For Biological Studies Hormone receptor compositions and methods
WO1992016546A1 (en) * 1991-03-19 1992-10-01 The Salk Institute For Biological Studies Response element compositions and assays employing same
WO1996040958A1 (en) * 1995-06-07 1996-12-19 Baylor College Of Medicine Nucleic acid transporters for delivery of nucleic acids into a cell
WO1999054728A2 (en) * 1998-04-23 1999-10-28 Karo Bio Usa, Inc. Method of predicting receptor modulating activity

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988003168A1 (en) * 1986-10-24 1988-05-05 The Salk Institute For Biological Studies Hormone receptor compositions and methods
WO1992016546A1 (en) * 1991-03-19 1992-10-01 The Salk Institute For Biological Studies Response element compositions and assays employing same
WO1996040958A1 (en) * 1995-06-07 1996-12-19 Baylor College Of Medicine Nucleic acid transporters for delivery of nucleic acids into a cell
WO1999054728A2 (en) * 1998-04-23 1999-10-28 Karo Bio Usa, Inc. Method of predicting receptor modulating activity

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
EVANS R M: "THE STERIOD AND THYROID HORMONE RECEPTOR SUPERFAMILY", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 240, no. 4854, 13 May 1988 (1988-05-13), pages 889 - 895, XP000574469, ISSN: 0036-8075 *
FUHRMANN U ET AL: "STABLE TRANSFECTION OF ANDROGEN RECEPTOR AND MMTV-CAT INTO MAMMALIAN CELLS: INHIBITION OF CAT EXPRESSION BY ANTI-ANDROGENS", JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, ELSEVIER SCIENCE LTD., OXFORD, GB, vol. 42, no. 8, September 1992 (1992-09-01), pages 787 - 793, XP000884986, ISSN: 0960-0760 *
GERDES MICHAEL J ET AL: "Transforming growth factor-beta1 induces nuclear to cytoplasmic distribution of androgen receptor and inhibits androgen response in prostate smooth muscle cells.", ENDOCRINOLOGY, vol. 139, no. 8, August 1998 (1998-08-01), pages 3569 - 3577, XP002193244, ISSN: 0013-7227 *
HOFBAUER LORENZ C ET AL: "Development and characterization of a conditionally immortalized human osteoblastic cell line stably transfected with the human androgen receptor gene.", JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 66, no. 4, 1997, pages 542 - 551, XP002193246, ISSN: 0730-2312 *
NEGRO-VILAR ANDRES: "Selective androgen receptor modulators (SARMs): A novel approach to androgen therapy for the new millennium.", JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, vol. 84, no. 10, October 1999 (1999-10-01), pages 3459 - 3462, XP002193249, ISSN: 0021-972X *
SALM SARAH N ET AL: "Transforming growth factor-beta is an autocrine mitogen for a novel androgen-responsive murine prostatic smooth muscle cell line, PSMC1.", JOURNAL OF CELLULAR PHYSIOLOGY, vol. 185, no. 3, December 2000 (2000-12-01), pages 416 - 424, XP001056974, ISSN: 0021-9541 *
SYMS A J ET AL: "MECHANISM OF ANDROGEN-RECEPTOR AUGMENTATION ANALYSIS OF RECEPTOR SYNTHESIS AND DEGRADATION BY THE DENSITY-SHIFT TECHNIQUE", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, no. 1, 1985, pages 455 - 461, XP001057030, ISSN: 0021-9258 *
TABB JOEL S ET AL: "Suppression of sodium channel function in differentiating C2 muscle cells stably overexpressing rat androgen receptors.", JOURNAL OF NEUROSCIENCE, vol. 14, no. 2, 1994, pages 763 - 773, XP001056898, ISSN: 0270-6474 *
TEROUANNE BEATRICE ET AL: "A stable prostatic bioluminescent cell line to investigate androgen and antiandrogen effects.", MOLECULAR AND CELLULAR ENDOCRINOLOGY, vol. 160, no. 1-2, 25 February 2000 (2000-02-25), pages 39 - 49, XP002193245, ISSN: 0303-7207 *
YARBROUGH W G ET AL: "A SINGLE BASE MUTATION IN THE ANDROGEN RECEPTOR GENE CAUSES ANDROGEN INSENSITIVITY IN THE TESTICULAR FEMINIZED RAT", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 265, no. 15, 1990, pages 8893 - 8900, XP001062675, ISSN: 0021-9258 *
ZHOU ZHIFENG ET AL: "Identification and characterization of a novel androgen response element composed of a direct repeat.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 13, 1997, pages 8227 - 8235, XP002193247, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2002000716A2 (en) 2002-01-03
MXPA02012573A (en) 2003-05-14
AU2001267008A1 (en) 2002-01-08
EP1297108A2 (en) 2003-04-02
JP2004517606A (en) 2004-06-17
CA2413596A1 (en) 2002-01-03

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