WO2001097943A1 - Dispositif de preparation des composants sanguins et procede d'utilisation dudit dispositif - Google Patents

Dispositif de preparation des composants sanguins et procede d'utilisation dudit dispositif Download PDF

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Publication number
WO2001097943A1
WO2001097943A1 PCT/US2001/041062 US0141062W WO0197943A1 WO 2001097943 A1 WO2001097943 A1 WO 2001097943A1 US 0141062 W US0141062 W US 0141062W WO 0197943 A1 WO0197943 A1 WO 0197943A1
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WO
WIPO (PCT)
Prior art keywords
bag
blood
whole blood
expressor
blood component
Prior art date
Application number
PCT/US2001/041062
Other languages
English (en)
Inventor
Glen Jorgensen
Original Assignee
Medicept, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medicept, Inc. filed Critical Medicept, Inc.
Publication of WO2001097943A1 publication Critical patent/WO2001097943A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0428—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles with flexible receptacles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/02—Blood transfusion apparatus
    • A61M1/0209—Multiple bag systems for separating or storing blood components
    • A61M1/0218—Multiple bag systems for separating or storing blood components with filters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3622—Extra-corporeal blood circuits with a cassette forming partially or totally the blood circuit
    • A61M1/36226—Constructional details of cassettes, e.g. specific details on material or shape
    • A61M1/362261—Constructional details of cassettes, e.g. specific details on material or shape at least one cassette surface or portion thereof being flexible, e.g. the cassette having a rigid base portion with preformed channels and being covered with a foil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3622—Extra-corporeal blood circuits with a cassette forming partially or totally the blood circuit
    • A61M1/36226—Constructional details of cassettes, e.g. specific details on material or shape
    • A61M1/362262—Details of incorporated reservoirs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3622—Extra-corporeal blood circuits with a cassette forming partially or totally the blood circuit
    • A61M1/36226—Constructional details of cassettes, e.g. specific details on material or shape
    • A61M1/362263—Details of incorporated filters
    • A61M1/362264—Details of incorporated filters the filter being a blood filter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • A61M1/3696—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • A61M1/3698—Expressing processed fluid out from the turning rotor using another fluid compressing the treatment chamber; Variable volume rotors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • B01D21/262—Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
    • B04B9/14—Balancing rotary bowls ; Schrappers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3622—Extra-corporeal blood circuits with a cassette forming partially or totally the blood circuit
    • A61M1/36226—Constructional details of cassettes, e.g. specific details on material or shape
    • A61M1/362266—Means for adding solutions or substances to the blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3627—Degassing devices; Buffer reservoirs; Drip chambers; Blood filters
    • A61M1/3633—Blood component filters, e.g. leukocyte filters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/12—General characteristics of the apparatus with interchangeable cassettes forming partially or totally the fluid circuit
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2221/00—Applications of separation devices
    • B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B04B2005/0478—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation with filters in the separation chamber
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
    • B04B9/14—Balancing rotary bowls ; Schrappers
    • B04B2009/143—Balancing rotary bowls ; Schrappers by weight compensation with liquids

Definitions

  • Red blood cells carry oxygen and usually are used to treat patients with anemia. For example, patients with chronic anemia resulting from disorders such as kidney failure, malignancies, or gastrointestinal bleeding and those with acute blood loss resulting from trauma or surgery.
  • White blood cells are responsible for protecting the body from invasion by foreign substances such as bacteria, fungi and viruses.
  • a common centrifuge process is described in the AABB Technical Manual, methods 9.4 and 9.11 as follows: Typically, the bag of whole blood is carefully loaded into one of the buckets of a large swinging bucket centrifuge. The opposing buckets are weighed and balanced so that their weight is within a few grams. Then, the buckets are loaded into a rotor and the rotor spun at conditions called "light spin" by the blood banking community (2000 g for 3 min).
  • the present invention provides an improved method and apparatus for the separation of whole blood into its components.
  • the method and apparatus automates the separation process, thereby dramatically reducing the labor involved in conventional separation of whole blood. Further, the method and apparatus allows for the separation of multiple units simultaneously, thereby dramatically reducing separation time.
  • the whole blood bag and blood component bags are fabricated of a material that allows them to expand and contract repeatedly to move fluids between the cavities, such as a flexible or an elastomeric material.
  • the number of blood component bags like the number of cavities, is not limited.
  • the bags for holding the whole blood and blood components are sterile bags fabricated of materials that are of the kind generally approved and accepted for that purpose. Preferably, these bags are shaped to fit the shape of the cassette caveties into which they are placed. Valves and sensors are preferably included to detect and control the flow of the components into the appropriate blood component bag.
  • an electronically driven solenoid valve can be used to stop the flow of plasma from being espressed from the whole blood bag as soon as red cells are optically detected in that stream, thereby signaling the end of the expression step.
  • Both the optic detector and the solenoid valve can be controlled by a microprocessor-based logic controller, preferably co-located in the hollow central drive shaft. Power for the optic detector and the solenoid valve can be fed into the rotating housing through a set of concentric slip rings. There is a practical limit on the number of separate power and signal lines that can be fed into the cassette. Therefore, a second type of valve is preferably used that does not require either power or signal communication to the controllers outside the rotating field. This second type of valve could be a centrifugally actuated valve that would open and close based on the speed of the rotor.
  • the stacked co-axial configuration operates as follows: a unit of whole blood is collected in a sterile whole blood bag. This whole blood bag is then connected to a sterile bag set via a sterile connection device. This bag set consists of the bags, tubing, and solutions necessary to separate the unit of whole blood into the desired components.
  • filters or columns are positioned in-line between the product bags in a manner that allows for the removal of target cells as they move from one bag to another.
  • filters or columns would preferably use an additional expression step.
  • An additional expression step includes expressing the packed red blood cell mass through a leukodepleting filter or column to a storage bag containing the appropriate storage solution.
  • Another example includes expressing the storage solution in the red cell mass to dilute the cells before expressing the mixture through the le ⁇ kodepleting filter.
  • Yet another example includes using a column to collect CD-34 stem cells from the plasma stream as it is being expressed from the collect bag to the plasma bag.
  • the secondary separation step takes place outside the centrifuge.
  • the device further includes a built-in refrigerated chamber for controlling the temperature of the cells during the filtering process.
  • sucrose-based storage solutions that are commonly added to separated blood components
  • other fluids such as sucrose-based storage solutions that are commonly added to separated blood components
  • bags and cavities are in fluid communication with the appropriate blood component bag(s) such that, for example, after the blood components have been separated and collected in the appropriate blood component bag(s), the storage solution can be added to the appropriate blood component bag(s).
  • the number of bags and cavities is limited only by the space available in the centrifuge and the space for flow streams within the cassette.
  • a radial segment configuration is utilized.
  • a large rotating drum (“rotor") is divided into pie-shaped segments, each housing a removable cassette comprised of multiple sections.
  • a bag containing the whole blood is placed in one section of the cassette.
  • the remaining sections of the cassette are used for the containment of the separated blood components.
  • the cassette consists of three segments wherein the inner segment contains a first expressor chamber, the middle segment contains both a second expressor chamber and a whole blood bag and the outer segment contains a platelet collection bag.
  • a final plasma collection bag can be positioned on an inside surface of the inner segment.
  • a pumping device is used to assist in moving fluid and components from one bag to another.
  • an auto-balancing mechanism which automatically compensates for the changing state of imbalance of the rotor, is connected to the rotor, thereby eliminating the need for additional balancing steps during the separation process.
  • the bag arrangements presented previously are shaped to fit into a large swinging-bucket rotor.
  • Swinging- bucket rotors have become common in blood component labs and, thus, this configuration would appeal to the market because labs could use the existing installed base of centrifuges for the process and apparatus of the present invention.
  • modifications would be required to both the rotor and the machine to allow for expressing fluid to enter the bucket and to position valves and optic detectors on the rotor.
  • Both the radial configuration and the swinging bucket configuration are used in a manner similar to that described above relating to the stacked disk configuration .
  • FIG. 1 is a schematic illustration of the separation activities in accordance with one embodiment of the present invention.
  • FIG. 2 is an artists rendering of one embodiment of the separation system in accordance with the present invention.
  • FIG. 3 is rendering of a typical cassette for the stacked-disk configuration in accordance with one embodiment of the present invention.
  • FIG. 4 is a sketch of the fluid management components housed inside the drive shaft in accordance with one embodiment of the present invention.
  • FIG. 5 is a rendering of the optional processing packs that can be used in the stacked disk configuration in accordance with one embodiment of the present invention.
  • FIG. 6 is the cassette of Fig. 3 including the mechanical components from Fig 4.
  • FIG. 7 is a rendering of the expressor chamber inside the drive shaft in accordance with one embodiment of the present invention.
  • FIG. 8 is a rendering of the self balancing feature of the stacked disk in accordance with one embodiment of the present invention.
  • FIG. 9 is a second embodiment of the stacked disk in accordance with the present invention.
  • FIG. 10 is a third embodiment of the stacked disk in accordance with the present invention.
  • FIG. 11 is a sketch of the alternative means for pumping fluids into the cassette in accordance with one embodiment of the present invention.
  • FIG. 12 is a sketch of the radial configuration in accordance with one embodiment of the present invention.
  • FIG. 13 is a sketch of the closed cassette for the radial configuration in accordance with one embodiment of the present invention.
  • FIG. 14 is a sketch of the open cassette for the radial configuration in accordance with one embodiment of the present invention.
  • FIG. 15 is a sketch of the bag set used in .the radial configuration in accordance with one embodiment of the present invention.
  • FIG. 16 is a sketch of the bag set from FIG 15 positioned in the cassette of FIG 14.
  • FIG. 17 is a sketch of section 5-5 through the cassette in FIG 16
  • FIG. 18 is a sketch of the self-balancing mechanism for the radial configuration in accordance with one embodiment of the present invention.
  • FIG. 19 is a sketch of the swinging bucket configuration in accordance with one embodiment of the present invention. DETAILED DESCRIPTION OF THE INVENTION
  • the automated blood fractionation device of the present invention separates whole blood into it three primary components, red blood cells, platelets, and plasma. These components are separated and transferred into various blood component bags through sealed lengths of tubing or a similar mechanism that interconnect the various blood bags.
  • a volume of whole blood is collected and placed into the device.
  • the collected whole blood is fed into the whole blood bag 6, which is then placed into the device.
  • the device holding the whole blood bag 6 is then is spun at high speeds to separate the red cells from the plasma.
  • the spinning whole blood bag 6 is preferably compressed in a way that allows the plasma to move from the whole blood bag 6 to the platelet concentrate bag 8 through tubing that interconnects the whole blood bag 6 and the platelet concentrate bag 8.
  • the plasma continues to move toward the platelet poor plasma bag 9.
  • the plasma contains a second cellular component, called platelets.
  • the platelets sediment radially and collect on the outermost wall, while the platelet poor plasma continues to and fills the plasma bag 9. This continues until all of the platelet-rich-plasma in the whole blood bad 6 has been squeezed out, or "expressed", from the whole blood bag 6.
  • red blood cells then begin to move out of the whole blood bag 6 until an optic detector 20 senses a color or turbidity shift (or both) and signals valve 21 to close and valve 22 to open. Then, as the expressing fluid or gas continues to squeeze the contents out of the whole blood bag 6, which now contains only red blood cells, these red blood cells flow into the red blood cell bag 7 until all have been expressed from the whole blood bag 6.
  • red blood cell bag 7 the red blood cells are preferably mixed with a fixed amount of storage solution that is pre-charged into the red blood cell bag 7.
  • the storage solution may be added to the red blood cells in the whole blood bag 6.
  • the above-described process can also be carried out as an ongoing procedure while the whole blood is being pumped into the whole blood bag 6 from an external source through, for example, a set of rotating face seals of an Adams- type skip rope.
  • Fig. 1 which contains a whole blood bag 6, red blood cell bag 7, platelet concentrate bag 8 and platelet poor plasma bag 9, not all of these bags are required for each process, multiple types of bags may be used, and additional, different bags than those described may be included.
  • an automated blood fractionation device in accord with one embodiment of the present invention has a stacked co-axial configuration.
  • a plurality of circular cassettes 1 are stacked in a co-axial configuration and placed over a drive shaft 2 within a centrifuge 3, which is designed to accommodate the cassettes 1.
  • This configuration is advantageous in that each cassette 1 is self-balancing irrespective of the difference in the displaced mass during the expression steps of several cassettes 1 simultaneously.
  • the circular cassettes 1 are constructed as shown in Fig. 3, so as to form a plurality of cavities that can be loaded with the whole blood bag 6 and the various blood component bags.
  • the various blood component bags may include a red blood cell bag 7, a platelet concentrate bag 8, and plasma bag 9.
  • Other cavities, such as cavity 10 may be included for yet undefined requirements, such as, for example, holding storage solution that is added to the packed red blood cells and, for example, for holding an expressor chamber or an expressor bag as described in further detail below.
  • Yet other cavities may be positioned to hold filters 12 and 13 (e.g. leukodepleting filters) and separation columns.
  • the cavities can be structured and configured such as those shown in the Figures or in any other manner to permit the various blood bags or other flexible containers to be placed into and removed from the cavities.
  • the blood component bags are in fluid communication with eachother with interconnecting tubing 14, or the like.
  • the tubing 14 is preferably positioned in recesses formed (e.g. molded) into the cassette in order to route the tubing 14 between cavities and secure the tubing against the centrifugal force to prevent collapsing or crimping of the tubing walls.
  • a vertical section 15 of the tubing, shown in Figs. 3 and 6, is preferably positioned in the cassette 1 so that it is visible from outside the closed cassette 1.
  • Means for detecting when the fractionation process is complete and a means for closing the interconnection between blood component bags also can be located within the device. For example, as shown in Figs.
  • an optic detector 20 can be used which senses the presence of red cells in the supernatant line of the tubing and signals a valve 21 to close and the pump (not shown) to stop. This will prevent contamination of the platelet and plasma in bags 9, 8 with red blood cells. Then, valve 22 can be opened and expression can resume to move the red blood cells from the whole blood bag 6 through the tubing 14 and into the red blood cell bag 7.
  • the cassettes 1 preferably further include an expressor chamber 23.
  • the expressor chamber 23 is sealed off by a flexible membrane 11.
  • the expressor chamber 23 and flexible membrane are preferably positioned in the cassette 1 adjacent to the portion of the cassette 1 that holds the whole blood bag and blood component bags.
  • the cassette 1 may be formed of two separable portions, one of which holds the various blood component bags and the other of which holds the expressor chamber 23. In one embodiment, shown in Figs. 3 and
  • a top portion 17 is attached to a bottom portion 18 with a fastening mechanism 19, such as a hinge or threaded surfaces along the circumference of the top portion 17 and bottom portion 18, such that the cassette 1 may be opened to expose the inside of the cassette 1.
  • a fastening mechanism 19 such as a hinge or threaded surfaces along the circumference of the top portion 17 and bottom portion 18, such that the cassette 1 may be opened to expose the inside of the cassette 1.
  • expressing fluid or gas is pumped into the expressor chamber 23 for the purpose of expanding the flexible membrane 11, which pressurizes one or more of the blood component bags.
  • the flexible membrane sealing the expressor chamber 23 is in wall-to-wall contact with one or more of the blood component bags.
  • the expressor chamber 23 has a fixed volume such that, as expressing fluid (liquid or gas) is pumped into the expressor chamber 23, the flexible membrane 11 expands against, for example, the whole blood bag 6, thereby squeezing and reducing the volume of the bag 6 and forcing material out of the bag 6.
  • the expressor chamber 23 is supplied with expressor fluid or gas from an external source, preferably through inlet/ outlet port 16. Pumping means [not shown] can be located either within the cassette 1 or outside the cassette 1 to further aid in moving materials from one blood bag to another.
  • the expressor chamber 23 is positioned such that the flexible membrane 11 is in wall-to-wall contact with the whole blood bag 6. As the centrifuge spins the cassettes 1 at high speeds, the red blood cells are separated from the plasma. Once the separation has occurred, expressor fluid or gas is fed into expressor chamber 23, thereby causing the flexible membrane 11 to expand and compress the whole blood bag 6. This forces the separated plasma to move from the whole blood bag 6 to the platelet concentrate bag 8 through tubing or a similar mechanism that interconnects the whole blood bag 6 and the platelet concentrate bag 8. After filling the platelet concentrate bag 8, the plasma continues to move toward the platelet poor plasma bag 9.
  • an expressor bag fabricated of a flexible, expandable material may be used.
  • Fig. 5 illustrates the configuration of a few of these activities. For example, if only red blood cells and plasma are collected, then a double pack set shown in 5a can be used. If red blood cells, plasma and platelets are collected, then a triple pack set shown in 5b is used. If the packed red blood cells will require additional operations, such as adding chemicals to prepare the red blood cells for freezing, viral inactivation, enzymatic conversion, and the like, then a secondary pack set shown in 5c can be used where the packed red blood cells are temporarily stored in a bag 50 suitable for use in the centrifuge again.
  • Leukodepleting filters 12, 13 that remove leukocytes from the packed red blood cells and platelet-rich-plasma, respectively, can be interconnected in the pack tubing arrangement. In all cases, it is preferable to collect the whole blood into a single whole blood bag 6 without regard for the ultimate activity for which the blood is being drawn. Then, just before processing, the appropriate pack set 5a, 5b, 5c is connected to the whole blood bad 6 by means of a sterile interlocking connector which consists of a female portion 36 sealed into the whole blood bag 6 and a male portion 37 sealed into the pack's connecting tube. ⁇
  • the method of using the stacked coaxial configuration is as follows: units of whole blood are collected in sterile whole blood bags 6. The whole blood bags 6 are then connected, while maintaining sterility, to the appropriate pack set 5a, 5b, 5c, as described above, and the various blood component bags are positioned in the appropriate cavities within the open cassettes 1 as described above. The cassettes 1 are then closed and loaded into the centrifuge 3.
  • the centrifuge 3 sediments the red blood cells at high speed to the outer portion of the whole blood bag 6.
  • expressor fluid or gas is pumped into the expressor chamber 23, thereby causing the flexible membrane 11 to expand against the whole blood bag 6.
  • This causes the plasma to flow from whole blood bag 6, past the optic detector 20, through open valve 21, through the platelet concentrate bag 8 to the platelet poor plasma bag 9.
  • the cavity that holds the platelet concentrate bag 8 is preferably sized to limit the amount of liquid held by the platelet concentrate bag 8 to a fixed volume (for example, 50 ml). The expression continues until the optic detector 20 detects the presence of red blood cells exiting the whole blood bag 6.
  • valve 21 closes and valve 22 opens to prevent red blood cells from passing into platelet concentrate bag 8 and the platelet poor plasma bag 9. Additional valves may be located upstream, for example, valve 22, which may open at this time. Expression then resumes to move the remaining red blood cells from the whole blood bag 6 into red blood cell bag 7.
  • the red blood cell bag may, if desired, be pre-charged with nutrient storage solution for extended storage of the red blood cells.
  • secondary separation devices such as filters 12, 13 (e.g. leukodepleting filters) or columns (not shown), are positioned within with the device in-line between the various blood component bags.
  • filters 12, 13 e.g. leukodepleting filters
  • columns not shown
  • filters 12, 13 e.g. leukodepleting filters
  • These secondary separation devices provide for the removal of target cells as they move from one bag to another.
  • the platelet rich plasma is expressed from the whole blood bag 6, it can be forced through leukodepleting filter 13 at a precise rate to optimize the filter's performance.
  • a leukodepleting filter 12 may also be placed inline with the inlet to the red blood cell bag 7.
  • the inlet and outlet axis of either or both filters 12, 13 may be positioned radially rather than tangentially as shown if Fig 6, so that the centrifugal force does not cause the fluid flow to be biased towards the radially most outboard position within the filter housing.
  • a column designed for the collection of CD-34 stem cells is positioned between the whole blood bag 6 and the plasma bag 9 such that the column collects CD-34 stem cells from the plasma stream as it is being expressed from the whole blood bag 6 to the plasma bag 9.
  • a column may be positioned in line with the red blood cells such that the red blood cells are passed through the column to remove residual processing chemicals (e.g. glycerol, which is used for cryopreservation).
  • these secondary separation steps may take place outside the centrifuge.
  • a built-in refrigerated chamber (not shown) is included for controlling the temperature of the cells during the filtering process.
  • the expressor fluid or gas may be transferred to the expressor chamber 23 from an external source through the inlet or port 60 which, in turn, is in fluid communication with a common supply header, 40, positioned within the drive shaft 2.
  • Cassettes 1 are preferably positioned onto the drive shaft 2 in pairs so that one is 180° from the other as shown in Fig. 8.
  • the plasma 71 that is expressed to the side of one cassette is mechanically balanced with the plasma 72 moving to the opposite side of the adjacent cassette.
  • the red blood cells 73 that are expressed to one side of one cassette are mechanically balanced by the red blood cells 74 moving to the opposite side of the adjacent cassette.
  • Fig. 9 shows a configuration where the cassette 1 is comprised of three segments: top segment 81 holds the whole blood bag 6, middle segment 82 holds the expressor chamber 23, flexible membrane 11 and filters 12, 13, and bottom segment 83 holds the platelet concentrate bag 8, the platelet poor plasma bag 9, and the red blood cell bag 7.
  • top segment 81 holds the whole blood bag 6
  • middle segment 82 holds the expressor chamber 23
  • flexible membrane 11 and filters 12, 13 holds the platelet concentrate bag 8, the platelet poor plasma bag 9, and the red blood cell bag 7.
  • the cassette 1 can be made more compact and can be used in a small, portable centrifuge where the diameter of the cassette 1 can be as small as 5-6 inches.
  • the three segment cassette can made larger, for example 12 inches in diameter, in which case the cassette 1 could carry over three liters of fluids in addition to the volume of the cells.
  • the deglycerolization of frozen red cells requires that approximately two liters of solutions be used to wash the cells before transfusion; hypertonic 12% NaCl, 1.6% NaCl, and resu spend in 0.9% saline with dextrose (Method 9.6 of the AABB Technical Manual 12 th Edition).
  • the three solutions can be carried "on board” to sequentially wash the red cells, loaded into the bags 7, 8, and 9 in Fig. 9.
  • Two expressor chambers 23 would be used to move the cells into and out of the red blood cell bag 7, and an additional valve would be added.
  • a common centrifuge would be used to process a multiplicity of cassette styles, each performing a different blood processing activity in the blood center.
  • Other examples include the washing or rejuvination of red cell cells (Method 9.5 of the AABB Technical Manual 12 th Edition) that requires 2 liters of unbuffered 0.9% saline, virally inactivated cells (approximately 2 liters), enzymatic conversion of red cells (approximately 3 liters), and others.
  • FIG. 10 Another variation of the radial configuration cassette is shown in Figs. 10.
  • This configuration contains a top portion 17 and a bottom portion 18.
  • the red blood cell bag 7 and platelet poor plasma bag 9 are positioned to be coaxial with each other and the whole blood bag 6.
  • the blood is collected and subsequently connected, while maintaining sterility, to a processing bag set, e.g. Fig 5, in the same manner as described above with the exception that as the bag set is positioned into cassette 1, the placement of the bags varies.
  • the whole blood bag 6 and expressor chamber 23 are placed into a cavity of the cassette. This chamber is in fluid communication with a supply of expressing fluid 39, the pressure of which is controlled by a pumping means outside of the cassette.
  • the top portion 17 of the cassette is placed over the bottom portion 18 to enclose the whole blood bag 6.
  • the top portion 17 contains cavities for the red blood cell bag 7, platelet poor plasma bag 9, and the platelet concentrate bag 8. Cavities can also be provided for one or more filters.
  • a platelet rich plasma filter 42 and a red blood cell leukofilter 41 are positioned in the cassette 1 as shown.
  • Channels are preferably provided to fix the routing of the interconnecting tubing 14 so that sensors (such as optic and pressure sensors) and valves 21 can reliably contact the tubing 14. These sensors and valves can be positioned within the cassette 1, or, preferably outside the cassette 1 as part of the centrifuge drive mechanism.
  • FIG. 11 Another embodiment of the invention pumps the whole blood (or other cell mass) into the cassettes 1 while the separation is taking place.
  • multiple lumens (tubes) 34 are connected to the separation chambers of one or more cassettes 1 housed in a centrifuge 3 preferably through either of two means: a multichannel face seal or an Adams-type skip rope.
  • the separation proceeds as described above, except that the additional blood that is continuously being pumped into the device displaces and forces the platelet-rich plasma out of the whole blood bag 6.
  • expressor fluid or gas can be pumped into the whole blood bag 6 through rotating seals 52 and feed tube 54 located in at the bottom of the centrifuge 3.
  • the expressor fluid or gas is removed from the whole blood bag 6, then additional fluids can be added to the cell mass in the whole blood bag 6 via the rotating seal 52 or multiple lumens (tubes) 34 and removed with the expressor fluid or gas as it is again pumped into the whole blood bag 6.
  • the liquid that is expressed after the components have been separated can be expressed out of the whole blood bag 6 through any one the multiple lumens (tubes) 34 and into a waste bag.
  • the number of cassettes is limited only by the strength of the closing mechanism that secures the cassettes 1 in the closed position during separation and the size of the centrifuge. If a small device is required, as few as one cassette can be used. If high throughput is required, a plurality of cassettes can be used.
  • Fig 11 Shown in Fig 11 is an alternate method for positioning the optics to detect the red blood cell interface during expression.
  • the optic sensor 20 is fixed to the non-rotating containment wall of the centrifuge 3.
  • the optic sensor 20 monitors the tubing 14 in the cassette 1 through a hole 56 in the cassette 1 that allows visualization of the length of tubing 14 that carries the plasma and red blood cells from the whole blood bag 6 to the platelet concentrate bag 8.
  • the sampling rate of the optic sensor 20 is such that it emits and receives an optic signal in less time than that which is required for the hole 20 to rotate past its field of view.
  • an automated blood fractionation device in accord with another embodiment of the present invention has a radial segment configuration.
  • a large rotating drum or a . rotor 1 is divided into pie-shaped segments 102.
  • a cassette 103 having a shape conforming to the radial segment configuration of the rotor can be inserted into each segment 102.
  • the cassette 103 is comprised of a plurality of sections 104, and each section can contain one or more cavities 105 for the containment of the fluids necessary to effect the fractionation process.
  • Cavities 105 can be structured and configured such as those shown or in any other manner to permit whole blood bags and various blood component bags or other flexible containers to be placed into and removed from the cavities 105.
  • the bags set including the whole blood bags 6, are then loaded into the cavities 105 in the cassette 103 (Fig. 12).
  • the number of sections 104 and cavities 105 required depends on the number of bags used in a given process.
  • the cassette 103 is closed.
  • a lid (not shown) of the cassette 103 can be attached, for example, on one side of the cassette with hinges or other fastening means (not shown) so that the cassette can be opened to expose all cavities and shut quickly.
  • the sections 104 can be connected with hinges or other fastening means 109 that allows the sections 104 to be separated from each other to expose the cavities 105.
  • the cassette 103 consists of three sections: an inner sectionl lO, a middle section 111 and an outer section 112.
  • the inner section 110 typically contains a first expressor reservoir 113 into which an expressor bag 7 can be placed.
  • the middle segment 111 contains both a second expressor reservoir 114, into which an expressor bag can be placed and, adjacent to the expressor reservoir, a whole blood cavity 115 into which a whole blood bag 6 can be placed.
  • the outer segment 112 contains a cavity for a platelet concentrate bag 8.
  • a final plasma bag 9 is positioned on the inside surface of the inner segment 110, as shown in FIG. 16.
  • the bags are interconnected by tubes 14 that allow fluid to flow from one bag to another.
  • a pumping means 119 can further be located within the device to aid in moving fluid and components from one bag to another.
  • a means for detecting when the fractionation process is complete and a means for closing the interconnection between bags also can be located within the cassette.
  • an optic detector 20 can be used, which senses the presence of red cells in the supernatant line and signals a valve 21 to close and the pump 119 to stop. This will prevent contamination between the contents of the various bags.
  • valves can be used that are either electronically powered or centrifugally actuated.
  • a single, electronic two-way valve can be used in place of two separate valves to take up less space and add fewer power leads.
  • the valves that are centrifugally actuated are preferred where it is desirable to eliminate the need for power connections
  • the expressor bags that are used within the cassette 103 are preferably fabricated of a material that allows them to expand and contract repeatedly to move fluids between the bags.
  • the expressor bags are fabricated of an elastomeric material such as, for example, silicone or natural rubber.
  • the expressor bags can be permanently installed in the cassette or, preferably, are removable.
  • the whole blood bags 6 are sterile bags into which whole blood is drawn and processed.
  • the whole blood bags 6 are fabricated of any type of material generally accepted and approved for that purpose.
  • the whole blood bags 6 are sized and shaped to fit readily into the appropriate cavity.
  • any shape may be used provided the whole blood bag 6 fits within the appropriate cavity of the cassette 103.
  • the number of cassettes 103, sections 104 and cavities 105 can vary depending on design choice. For example, fewer or more cassettes 103, sections 104 and cavities 105 can be used depending, for example, on the number of whole blood bags being fractionated and the number of components into which the whole blood is to be separated.
  • the method of using the radial segment configuration is as follows: units of whole blood are collected in sterile whole blood bags 6. The whole blood bags 6 are then positioned in the appropriate cavities 105 within the cassettes 103 as described above. The cassettes 103 are closed and loaded into the segments 102 in the centrifuge 3. Under centrifugal force, the red blood cells sediment radially outward in the whole blood bag 6.
  • expressor fluid or gas is pumped from the first reservoir 113 to the second reservoir 114, which compresses the whole blood bag 6 and forces the supernatant fluid (platelet rich plasma) through the platelet concentrate bag 8 and into the plasma bag 9.
  • the platelets sediment to the outer surface and are collected in the platelet concentrate bag 8. This expression continues until all of the supernatant has been expressed from the whole blood bag 6.
  • an optic detector 20 senses the presence of red cells in the supernatant line and signals a valve 21 to close and the expressor pump 119 to stop. This prevents any red cells from contaminating the downstream bags.
  • the centrifuge can then be stopped and the cassettes 103 removed and opened.
  • the bags are then separated and placed in the appropriate storage containers.
  • filters or packed columns or other secondary separation devices are positioned such that when the blood component bags are removed from the device, they have attached to them the secondary separation device and a receiving/ storage bag.
  • This allows the product bag to be hung in a temperature-controlled environment and the product slowly gravity drained through the secondary separation device into the final receiving/storage bag, which might contain nutrient solutions for long-term storage.
  • the centrifugal forces would not interfere with the function of the filter or column and the secondary separation step, which is relatively slow, does not tie up the centrifuge, thereby increasing throughput.
  • the secondary separation devices are positioned in line such that separation would occur as the fluids are expressed from one blood component bag to another as set out above.
  • filters or columns can be positioned between the blood component bags, as set out above, to provide for the removal of target cells as they move from one blood component bag to another.
  • sucrose-based storage solutions can be included through the addition of extra bags and cavities.
  • the number of bags and cavities is limited only by the space available in the rotor segment and the safety of flow streams within the cassette.
  • any number of bags, sections 102, cassettes 103, segments 104 and cavities 105 is within the scope of the invention.
  • the present invention preferably has an auto-balancing mechanism 142, shown in Fig 18, connected to the centrifuge 3.
  • an auto-balancing mechanism 142 shown in Fig 18, connected to the centrifuge 3.
  • accelerometers it is common to fix accelerometers to centrifuge assemblies to define the magnitude and the angle of the resulting imbalance vector for a rotating body such as that described herein.
  • Similar accelerometers can be fixed to the centrifuge frame that supports the centrifuge 3 of the present invention. These accelerometers are positioned to capture readings only in the single plane of the bottom surface of the centrifuge 3.
  • a software algorithm then can be used to interpret this data, calculate the magnitude and angle of the imbalance, and signal a set of three linear actuator motors, as shown in FIG. 18, to move to a calculated position that results in an opposing force equal and opposite to that experienced from the imbalance. This effectively cancels out the imbalance effects and assures smooth running and reliable separation of the cells from the suspending plasma.
  • Other mass distribution methods for canceling the imbalance also can be employed, such as pumping compensating fluid volumes to specific centrifuge locations.
  • the set of blood component bags is configured to fit into an existing piece of equipment that is already in the blood separations laboratory.
  • FIG 19A shows the layout of a multiple bucket set, while FIG 19B illustrates a typical way to apply the aforementioned inventions to this swinging bucket.
  • This embodiment operates to separate the blood components in a manner similar to the embodiments set out above.

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Abstract

L'invention concerne un procédé et un appareil de séparation sanguine automatisée permettant la séparation simultanée d'unités sanguines multiples en composants sanguins. Une fonction d'auto-compensation intégrée à cet appareil compense automatiquement l'état changeant du déséquilibre, éliminant ainsi la nécessité de toute étape de compensation supplémentaire au cours du processus de séparation. Cet appareil présente une pluralité de cassettes (1) situées sur un arbre d'entraînement (2). Ces cassettes (1) comportent un certain nombre de sections de confinement du sang entier et des composants sanguins séparés, qui sont contenus dans des poches jetables. L'arbre d'entraînement (2) est placé dans une centrifugeuse (3) et les composants sanguins sont séparés puis transférés aux poches situées dans les sections des cassettes (1). Des dispositifs de séparation auxiliaires tels que des filtres peuvent également être utilisés. Des informations de fabrication relatives aux identités de lots utilisées et aux conditions sous lesquelles chaque unité a été traitée sont également fournies.
PCT/US2001/041062 2000-06-20 2001-06-20 Dispositif de preparation des composants sanguins et procede d'utilisation dudit dispositif WO2001097943A1 (fr)

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WO2003086640A1 (fr) * 2002-04-12 2003-10-23 Gambro, Inc. Separation de fluides au moyen d'une centrifugeuse et d'une pompe a galets
WO2003089027A2 (fr) * 2002-04-16 2003-10-30 Gambro, Inc. Systeme, appareil et procede de traitement des composants sanguins
WO2004018021A2 (fr) * 2002-08-23 2004-03-04 Gambro, Inc. Methodes et dispositifs de separation de constituants sanguins
US6994790B2 (en) 2002-02-01 2006-02-07 Gambro, Inc. Whole blood collection and processing method
US7347932B2 (en) 2003-08-25 2008-03-25 Gambro Bct, Inc. Apparatus and method for separating a volume of composite liquid into at least two components
US7803101B2 (en) 2007-01-30 2010-09-28 Ortho-Clinical Diagnostics, Inc. Random access multi-disc centrifuge
US7819793B2 (en) 2006-06-07 2010-10-26 Caridianbct, Inc. Apparatus for separating a composite liquid into at least two components
US7833185B2 (en) 2004-12-28 2010-11-16 Caridianbct, Inc. Apparatus for separating a volume of whole blood into at least three components
WO2011071643A1 (fr) * 2009-12-08 2011-06-16 Caridianbct, Inc. Appareil de traitement du sang à unités multiples avec des compartiments progressivement centrés
US7981019B2 (en) 2005-08-22 2011-07-19 Caridianbct, Inc. Apparatus and method for separating a composite liquid into at least two components
US8120760B2 (en) 2008-07-31 2012-02-21 Caridianbct, Inc. Method and apparatus for separating a composite liquid into at least two components and for determining the yield of at least one component
US8173027B2 (en) 2006-09-06 2012-05-08 Terumo Bct, Inc. Method of separating a composite liquid into at least two components
US8236184B2 (en) 2007-05-14 2012-08-07 Terumo Bct, Inc. Method for separating a composite liquid into at least two components
US8287742B2 (en) 2006-12-20 2012-10-16 Terumo Bct, Inc. Method for separating a composite liquid into at least two components
US9079194B2 (en) 2010-07-19 2015-07-14 Terumo Bct, Inc. Centrifuge for processing blood and blood components
US9248446B2 (en) 2013-02-18 2016-02-02 Terumo Bct, Inc. System for blood separation with a separation chamber having an internal gravity valve

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US5547591A (en) * 1993-06-01 1996-08-20 Asahi Medical Co., Ltd. Method for separating a blood material into blood components by centrifugation, and centrifugal apparatus

Cited By (32)

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Publication number Priority date Publication date Assignee Title
US6605223B2 (en) 2000-06-20 2003-08-12 Medicept, Inc. Blood component preparation (BCP) device and method of use thereof
US6994790B2 (en) 2002-02-01 2006-02-07 Gambro, Inc. Whole blood collection and processing method
WO2003086640A1 (fr) * 2002-04-12 2003-10-23 Gambro, Inc. Separation de fluides au moyen d'une centrifugeuse et d'une pompe a galets
US7582049B2 (en) 2002-04-12 2009-09-01 Caridianbct, Inc. Fluid separation devices, systems and/or methods using a centrifuge and roller pump
US7033512B2 (en) 2002-04-12 2006-04-25 Gambro, Inc Fluid separation devices, systems and/or methods using a centrifuge and roller pump
US7708889B2 (en) 2002-04-16 2010-05-04 Caridianbct, Inc. Blood component processing system method
US7413665B2 (en) 2002-04-16 2008-08-19 Gambro Bct, Inc. Methods and apparatus for blood component separation
WO2003089027A2 (fr) * 2002-04-16 2003-10-30 Gambro, Inc. Systeme, appareil et procede de traitement des composants sanguins
US7648452B2 (en) 2002-04-16 2010-01-19 CardianBCT, Inc. Apparatus for blood component separation
US7166217B2 (en) 2002-04-16 2007-01-23 Gambro Inc Methods and apparatuses for blood component separation
WO2003089027A3 (fr) * 2002-04-16 2004-02-12 Gambro Inc Systeme, appareil et procede de traitement des composants sanguins
US7396451B2 (en) 2002-04-16 2008-07-08 Gambo Bci, Inc. Methods and apparatus for blood component separation
JP2005536293A (ja) * 2002-08-23 2005-12-02 ガンブロ　　インコーポレーテッド 血液成分分離のための方法および装置
WO2004018021A2 (fr) * 2002-08-23 2004-03-04 Gambro, Inc. Methodes et dispositifs de separation de constituants sanguins
WO2004018021A3 (fr) * 2002-08-23 2004-10-14 Gambro Inc Methodes et dispositifs de separation de constituants sanguins
JP4800617B2 (ja) * 2002-08-23 2011-10-26 カリディアンビーシーティー、インコーポレーテッド 血液成分分離のための方法および装置
US7347932B2 (en) 2003-08-25 2008-03-25 Gambro Bct, Inc. Apparatus and method for separating a volume of composite liquid into at least two components
US7648639B2 (en) 2003-08-25 2010-01-19 CaridianBCT, Inc Method for separating a volume of composite liquid into at least two components
US8277406B2 (en) 2004-12-28 2012-10-02 Terumo Bct, Inc. Method for separating a volume of whole blood into at least three components
US7833185B2 (en) 2004-12-28 2010-11-16 Caridianbct, Inc. Apparatus for separating a volume of whole blood into at least three components
US8057377B2 (en) 2005-08-22 2011-11-15 CaridianBCT, Inc Apparatus and method for separating a composite liquid into at least two components
US7981019B2 (en) 2005-08-22 2011-07-19 Caridianbct, Inc. Apparatus and method for separating a composite liquid into at least two components
US7819793B2 (en) 2006-06-07 2010-10-26 Caridianbct, Inc. Apparatus for separating a composite liquid into at least two components
US8173027B2 (en) 2006-09-06 2012-05-08 Terumo Bct, Inc. Method of separating a composite liquid into at least two components
US8287742B2 (en) 2006-12-20 2012-10-16 Terumo Bct, Inc. Method for separating a composite liquid into at least two components
US7803101B2 (en) 2007-01-30 2010-09-28 Ortho-Clinical Diagnostics, Inc. Random access multi-disc centrifuge
US8236184B2 (en) 2007-05-14 2012-08-07 Terumo Bct, Inc. Method for separating a composite liquid into at least two components
US8120760B2 (en) 2008-07-31 2012-02-21 Caridianbct, Inc. Method and apparatus for separating a composite liquid into at least two components and for determining the yield of at least one component
WO2011071643A1 (fr) * 2009-12-08 2011-06-16 Caridianbct, Inc. Appareil de traitement du sang à unités multiples avec des compartiments progressivement centrés
CN102655895A (zh) * 2009-12-08 2012-09-05 泰尔茂比司特公司 具有逐渐集中的室的多单元血液处理器
US9079194B2 (en) 2010-07-19 2015-07-14 Terumo Bct, Inc. Centrifuge for processing blood and blood components
US9248446B2 (en) 2013-02-18 2016-02-02 Terumo Bct, Inc. System for blood separation with a separation chamber having an internal gravity valve

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