WO2001096594A3 - Testsystem zur bestimmung der aktivität von cyclo-nukleotid-abhängigen proteinkinasen und vasp-phosphatasen - Google Patents

Testsystem zur bestimmung der aktivität von cyclo-nukleotid-abhängigen proteinkinasen und vasp-phosphatasen Download PDF

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Publication number
WO2001096594A3
WO2001096594A3 PCT/EP2001/006621 EP0106621W WO0196594A3 WO 2001096594 A3 WO2001096594 A3 WO 2001096594A3 EP 0106621 W EP0106621 W EP 0106621W WO 0196594 A3 WO0196594 A3 WO 0196594A3
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WO
WIPO (PCT)
Prior art keywords
vasp
activity
appropriate
cnpk
cyclo
Prior art date
Application number
PCT/EP2001/006621
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English (en)
French (fr)
Other versions
WO2001096594A2 (de
Inventor
Peter Drueckes
Thomas Jarchau
Ulrich Walter
Benjamin Bader
Original Assignee
Vasopharm Biotech Gmbh
Peter Drueckes
Thomas Jarchau
Ulrich Walter
Benjamin Bader
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vasopharm Biotech Gmbh, Peter Drueckes, Thomas Jarchau, Ulrich Walter, Benjamin Bader filed Critical Vasopharm Biotech Gmbh
Priority to EP01938257A priority Critical patent/EP1294926A2/de
Priority to AU2001263956A priority patent/AU2001263956A1/en
Publication of WO2001096594A2 publication Critical patent/WO2001096594A2/de
Publication of WO2001096594A3 publication Critical patent/WO2001096594A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Beschrieben wird ein HTS-geeignetes Testsystem zum Nachweis der Aktivität von cyclo-Nucleotid-abhängigen Proteinkinasen (cNPK) enthaltend a) mindestens eine Testverbindung, b) mindestens ein geeignetes cNPK-Substrat, c) mindestens eine zu inkubierende Zusammensetzung enthaltend cNPK und ATP, ggf. Phosphorylierungsreaktionsstopper, und d) ein geeignetes Detektionssytem zur Quantifizierung der Phosphorylierung des cNPK-Substrats. Ferner wird ein HTS-geeignetes Testsystem zum Nachweis der Aktivität von VASP-Phosphatasen beschrieben enthaltend e) mindestens eine Testverbindung, f) mindestens ein geeignetes VASP-Phosphatase-Substrat, g) mindestens eine zu inkubierende Zusammensetzung enthaltend VASP-Phosphatase, und h) ein geeignetes Detektionssystem zur Quantifizierung der Dephosphorylierung des VASP-Phosphatase-Substrats. Die beschriebenen Testsysteme lassen sich zum Auffinden von Verbindungen, die die Aktivität einer cNPK oder einer VASP-Phosphatase modulieren, aus chemischen oder natürlichen Substanzbibliotheken einsetzen.
PCT/EP2001/006621 2000-06-14 2001-06-12 Testsystem zur bestimmung der aktivität von cyclo-nukleotid-abhängigen proteinkinasen und vasp-phosphatasen WO2001096594A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01938257A EP1294926A2 (de) 2000-06-14 2001-06-12 Testsystem zur bestimmung der aktivität von cyclo-nukleotid-abhängigen proteinkinasen und vasp-phosphatasen
AU2001263956A AU2001263956A1 (en) 2000-06-14 2001-06-12 Test system for determining the activity of cyclo-nucleotide-dependent protein kinases and vasp phosphatases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10029210.0 2000-06-14
DE10029210A DE10029210A1 (de) 2000-06-14 2000-06-14 Testsystem sowie dessen Verwendung

Publications (2)

Publication Number Publication Date
WO2001096594A2 WO2001096594A2 (de) 2001-12-20
WO2001096594A3 true WO2001096594A3 (de) 2002-05-16

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/006621 WO2001096594A2 (de) 2000-06-14 2001-06-12 Testsystem zur bestimmung der aktivität von cyclo-nukleotid-abhängigen proteinkinasen und vasp-phosphatasen

Country Status (5)

Country Link
US (1) US20030166005A1 (de)
EP (1) EP1294926A2 (de)
AU (1) AU2001263956A1 (de)
DE (1) DE10029210A1 (de)
WO (1) WO2001096594A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10058596A1 (de) * 2000-11-25 2002-06-06 Aventis Pharma Gmbh Verfahren zum Screening von chemischen Verbindungen zur Modulierung der Wechselwirkung einer EVH1-Domäne oder eines Proteins mit einer EVH1-Domäne mit einer EVH1-Bindedomäne oder einem Protein mit einer EVH1-Bindedomäne sowie ein Verfahren zum Nachweis besagter Wechselwirkung
CA2445420A1 (en) * 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
US20070264678A1 (en) 2005-10-28 2007-11-15 Invitrogen Corporation Kinase and ubiquination assays
EP3091359A1 (de) * 2015-05-08 2016-11-09 Leibniz - Institut für Analytische Wissenschaften - ISAS - E.V. Verfahren zur identifizierung von markerproteinen zur diagnose und risikostratifizierung von störungen der blutgerinnung

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999009199A2 (en) * 1997-08-20 1999-02-25 University Of Medicine And Dentistry Of New Jersey Elongation factor-2 kinase (ef-2 kinase) and methods of use therefor
WO1999024473A1 (en) * 1997-11-07 1999-05-20 Aventis Pharma Deutschland Gmbh Antibodies against phosphorylated vasp (vasodilator-stimulated phosphoprotein), hybridoma cells for their preparation, and their use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999009199A2 (en) * 1997-08-20 1999-02-25 University Of Medicine And Dentistry Of New Jersey Elongation factor-2 kinase (ef-2 kinase) and methods of use therefor
WO1999024473A1 (en) * 1997-11-07 1999-05-20 Aventis Pharma Deutschland Gmbh Antibodies against phosphorylated vasp (vasodilator-stimulated phosphoprotein), hybridoma cells for their preparation, and their use

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ABEL ET AL: "Monoclonal antibodies against the focal adhesion protein VASP revealing epitopes involved in the interaction with two VASP binding proteins and VASP phosphorylation", EUROPEAN JOURNAL OF CELL BIOLOGY, WISSENSCHAFLICHE VERLAGSGESELLSCHAFT, STUTTGART,, DE, vol. 69, 1996, pages 39, XP002097463, ISSN: 0171-9335 *
BUTT ET AL: "cAMP- and cGMP-dependent protein kinase phosphorylation sites o the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 269, no. 20, 20 May 1994 (1994-05-20), pages 14509 - 14517, XP002097404, ISSN: 0021-9258 *
DOSTMANN WOLFGANG R G ET AL: "Delineation of selective cyclic GMP-dependent protein kinase Ialpha substrate and inhibitor peptides based on combinatorial peptide libraries on paper.", PHARMACOLOGY & THERAPEUTICS, vol. 82, no. 2-3, May 1999 (1999-05-01), pages 373 - 387, XP001066204, ISSN: 0163-7258 *
JARCHAU T ET AL: "Purification and assays of vasodilator - stimulated phosphoprotein", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 298, 1998, pages 103 - 113, XP002097408, ISSN: 0076-6879 *
SMOLENSKI ET AL: "Analysis and regulation of vasodilator - stimulated phosphoprotein serine 239 phosphorylation in vitro and in intact cells using a phosphospecific monoclonal antibody", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 273, no. 32, 7 August 1998 (1998-08-07), pages 20029 - 20035, XP002097407, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2001096594A2 (de) 2001-12-20
EP1294926A2 (de) 2003-03-26
DE10029210A1 (de) 2002-01-31
US20030166005A1 (en) 2003-09-04
AU2001263956A1 (en) 2001-12-24

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