WO2001094375A1 - Canal potassique a grande conductance dependant du calcium utilise comme modulateur des effets et de la consommation d'alcool - Google Patents

Canal potassique a grande conductance dependant du calcium utilise comme modulateur des effets et de la consommation d'alcool Download PDF

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Publication number
WO2001094375A1
WO2001094375A1 PCT/US2001/017812 US0117812W WO0194375A1 WO 2001094375 A1 WO2001094375 A1 WO 2001094375A1 US 0117812 W US0117812 W US 0117812W WO 0194375 A1 WO0194375 A1 WO 0194375A1
Authority
WO
WIPO (PCT)
Prior art keywords
ethanol
channel
test
activation
effect
Prior art date
Application number
PCT/US2001/017812
Other languages
English (en)
Inventor
Steven L. Mcintire
Andrew G. Davies
Hongkyun Kim
Original Assignee
Mcintire Steven L
Davies Andrew G
Hongkyun Kim
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mcintire Steven L, Davies Andrew G, Hongkyun Kim filed Critical Mcintire Steven L
Priority to AU2001266653A priority Critical patent/AU2001266653A1/en
Priority to US10/297,269 priority patent/US20030211547A1/en
Publication of WO2001094375A1 publication Critical patent/WO2001094375A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Definitions

  • Substances that alter ethanol-induced activation of BK channels without affecting normal BK channel function are preferred. Such substances might also be identified using a cell culture system or yeast that express the subunit of BK channel of interest. If such BK channel also has a ⁇ subunit associated with it, then the cultured cells or yeast or oocytes could express both subunits. Cells expressing the proteins could be exposed to the substances to be tested in combination with ethanol. Additionally, channel activation will result in an efflux of potassium, which can be measured by means of a potassium-sensitive dye such as PBFI (Mevwis et al., Biophys. J.
  • PBFI potassium-sensitive dye

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La présente invention concerne l'identification du canal BK comme cible de médicaments qui modulent les effets de l'alcool éthylique et la consommation d'alcool éthylique. L'invention concerne également l'utilisation de modulateurs du canal BK dans le but de moduler la consommation d'alcool et les effets de l'alcool.
PCT/US2001/017812 2000-06-02 2001-06-01 Canal potassique a grande conductance dependant du calcium utilise comme modulateur des effets et de la consommation d'alcool WO2001094375A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001266653A AU2001266653A1 (en) 2000-06-02 2001-06-01 Large conductance calcium-dependent potassium channel as modulator of alcohol effects and consumption
US10/297,269 US20030211547A1 (en) 2001-06-01 2001-06-01 Large conductance calcium-dependent potassium channel as modulator of alcohol effects and consumption

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20943200P 2000-06-02 2000-06-02
US60/209,432 2000-06-02

Publications (1)

Publication Number Publication Date
WO2001094375A1 true WO2001094375A1 (fr) 2001-12-13

Family

ID=22778729

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/017812 WO2001094375A1 (fr) 2000-06-02 2001-06-01 Canal potassique a grande conductance dependant du calcium utilise comme modulateur des effets et de la consommation d'alcool

Country Status (2)

Country Link
AU (1) AU2001266653A1 (fr)
WO (1) WO2001094375A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1400805A1 (fr) * 2002-09-19 2004-03-24 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Appareil de mesure et méthode pour le criblage des cellules immobilisées sur des électrodes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DOPICO ET AL.: "Ethanol increases the activity of Ca++-dependent K+ (mslo) channels: Functional interaction with cytosolic Ca++", JPET, vol. 284, no. 1, 1997, pages 258 - 268, XP002947015 *
JAKAB ET AL.: "Ethanol activates maxi Ca2+-activated K+ channels of clonal pituitary (GH3) cells", J. MEMBR. BIOL., vol. 157, 1997, pages 237 - 245, XP002947016 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1400805A1 (fr) * 2002-09-19 2004-03-24 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Appareil de mesure et méthode pour le criblage des cellules immobilisées sur des électrodes
US7326539B2 (en) 2002-09-19 2008-02-05 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Measurement device and method for screening with cells immobilized on electrodes

Also Published As

Publication number Publication date
AU2001266653A1 (en) 2001-12-17

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