WO2001092489A3 - Gene desaturase palmitate - Google Patents

Gene desaturase palmitate Download PDF

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Publication number
WO2001092489A3
WO2001092489A3 PCT/US2001/017219 US0117219W WO0192489A3 WO 2001092489 A3 WO2001092489 A3 WO 2001092489A3 US 0117219 W US0117219 W US 0117219W WO 0192489 A3 WO0192489 A3 WO 0192489A3
Authority
WO
WIPO (PCT)
Prior art keywords
palmitate
disclosed
desaturase
desaturase gene
enzymes
Prior art date
Application number
PCT/US2001/017219
Other languages
English (en)
Other versions
WO2001092489A2 (fr
Inventor
John A Browse
Jennifer L Watts
Original Assignee
Univ Washington
John A Browse
Jennifer L Watts
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Washington, John A Browse, Jennifer L Watts filed Critical Univ Washington
Priority to AU2001263473A priority Critical patent/AU2001263473A1/en
Publication of WO2001092489A2 publication Critical patent/WO2001092489A2/fr
Publication of WO2001092489A3 publication Critical patent/WO2001092489A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

L'invention concerne des enzymes désaturases, et plus spécialement des Δ9 désaturases animales, ainsi que l'utilisation des ces enzymes pour modifier la saturation des acides gras, notamment la saturation des acides gras de graines oléagineuses. L'invention concerne plus particulièrement l'enzyme désaturase palmitate FAT-5. L'invention concerne également des séquences d'acides nucléiques codant pour les Δ9 désaturases animales, y compris des séquences codant pour FAT-5.
PCT/US2001/017219 2000-05-26 2001-05-25 Gene desaturase palmitate WO2001092489A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001263473A AU2001263473A1 (en) 2000-05-26 2001-05-25 Palmitate desaturase gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20769900P 2000-05-26 2000-05-26
US60/207,699 2000-05-26

Publications (2)

Publication Number Publication Date
WO2001092489A2 WO2001092489A2 (fr) 2001-12-06
WO2001092489A3 true WO2001092489A3 (fr) 2002-05-10

Family

ID=22771632

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/017219 WO2001092489A2 (fr) 2000-05-26 2001-05-25 Gene desaturase palmitate

Country Status (2)

Country Link
AU (1) AU2001263473A1 (fr)
WO (1) WO2001092489A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8853432B2 (en) 2004-04-22 2014-10-07 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cell

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10208812A1 (de) * 2002-03-01 2003-09-11 Basf Plant Science Gmbh Verfahren zur Herstellung von ungesättigten Fettsäuren
US7700833B2 (en) 2002-03-01 2010-04-20 Cornell University Process for the production of unsaturated fatty acids
CA2563875C (fr) 2004-04-22 2015-06-30 Commonwealth Scientific And Industrial Research Organisation Synthese d'acides gras polyinsatures a chaine longue par des cellules de recombinaison
FI118339B (fi) 2004-09-21 2007-10-15 Ab Enzymes Oy Uusi lakkaasientsyymi ja sen käyttö
RU2009111266A (ru) 2006-08-29 2010-10-10 Коммонвелт Сайентифик энд Индастриал Рисерч Организейшн (AU) Синтез жирных кислот
EP2358882B1 (fr) 2008-11-18 2017-07-26 Commonwealth Scientific and Industrial Research Organisation Enzymes et méthodes de production d'acides gras oméga-3
EP2861059B1 (fr) 2012-06-15 2017-05-03 Commonwealth Scientific and Industrial Research Organisation Production d'acides gras polyinsaturés à chaîne longue dans des cellules végétales
NZ721036A (en) 2013-12-18 2023-07-28 Grains Res & Dev Corp Lipid comprising long chain polyunsaturated fatty acids
CN105219789B (zh) 2014-06-27 2023-04-07 联邦科学技术研究组织 包含二十二碳五烯酸的提取的植物脂质

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999033958A2 (fr) * 1997-12-23 1999-07-08 University Of Bristol Desaturase
WO1999050430A2 (fr) * 1998-03-30 1999-10-07 Dow Agrosciences Llc Modification d'acide gras dans les plantes par expression d'une aspergillus nidulans delta-9 coa desaturase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999033958A2 (fr) * 1997-12-23 1999-07-08 University Of Bristol Desaturase
WO1999050430A2 (fr) * 1998-03-30 1999-10-07 Dow Agrosciences Llc Modification d'acide gras dans les plantes par expression d'une aspergillus nidulans delta-9 coa desaturase

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DATABASE EM_INV EBI, Hinxton, UK; 11 September 1997 (1997-09-11), WILSON R ET AL.: "2.2. Mb of contiguous nucleotide sequence from chromosome III of C. elegans", XP002191666 *
DATABASE EM_INV EBI, Hinxton, UK; 6 May 1997 (1997-05-06), LENNARD N: "Genome sequence of the nematode C. elegans: a platform for investigating biology", XP002191665 *
DATABASE EM_INV EBI, Hinxton, UK; 6 November 1996 (1996-11-06), BASHAMV M: "Genome sequence of the nematode C. elegans: a platform for investigating biology", XP002191664 *
DATABASE EM_INV EBI, Hinxton, UK; 7 August 2000 (2000-08-07), WATTS J L AND BROWSE J, XP002191670 *
DATABASE EM_INV EBI, Hinxton, UK; 7 August 2000 (2000-08-07), WATTS J L AND BROWSE J, XP002191671 *
DATABASE EM_INV EBI, Hinxton, UK; 7 August 2000 (2000-08-07), WATTS J L AND BROWSE J, XP002191672 *
GRAYBURN WS ET AL: "Fatty acid alteration by a delta 9 desaturase in transgenic tobacco tissue", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 10, 1 June 1992 (1992-06-01), pages 675 - 678, XP002089852, ISSN: 0733-222X *
SCIENCE, vol. 282, 1998, pages 2012 - 2018 *
WATTS JENNIFER L ET AL: "A palmitoyl-CoA-specific DELTA9 fatty acid desaturase from Caenorhabditis elegans.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 272, no. 1, 27 May 2000 (2000-05-27), pages 263 - 269, XP002191663, ISSN: 0006-291X *
WILSON R ET AL., NATURE, vol. 368, 1994, pages 32 - 38 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8853432B2 (en) 2004-04-22 2014-10-07 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cell

Also Published As

Publication number Publication date
WO2001092489A2 (fr) 2001-12-06
AU2001263473A1 (en) 2001-12-11

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