WO2001084949A2 - Protein and lipid sources for use in aquafeeds and animal feeds and a process for their preparation - Google Patents
Protein and lipid sources for use in aquafeeds and animal feeds and a process for their preparation Download PDFInfo
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- WO2001084949A2 WO2001084949A2 PCT/CA2001/000663 CA0100663W WO0184949A2 WO 2001084949 A2 WO2001084949 A2 WO 2001084949A2 CA 0100663 W CA0100663 W CA 0100663W WO 0184949 A2 WO0184949 A2 WO 0184949A2
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- oilseed
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- seed
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/001—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
- A23J1/002—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste from animal waste materials
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/142—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by extracting with organic solvents
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/06—Production of fats or fatty oils from raw materials by pressing
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/141—Feedstock
- Y02P20/145—Feedstock the feedstock being materials of biological origin
Definitions
- the present invention relates to a novel process for the production of nutritionally upgraded protein and lipid sources for use in aquafeeds and other animal feeds. More specifically, the present invention relates to a process involving the co-processing of animal offal(s) with oilseed(s); the invention also relates to products produced thereby.
- the invention relates to cold pressed plant oils suitable for organic human ' foods, as well as products for use as components in organic fertilizers, both produced by the process of the invention.
- Feed accounts for on average 35-60% of the operating costs of salmon farms and it represents the largest cost in the culture of other carnivorous aquatic species. Moreover, the protein sources presently used account for about 51 % of the total feed cost and this percentage can be higher than this when increased reliance is placed on imported premium quality fish meals. The latter mainly originate from South America through the processing of whole pelagic fish species like sardines and anchovies and they are used to meet most of the dietary protein needs of farmed Canadian salmon. Accordingly, salmon farming profitability is marginal in Canada.
- aquatic feeds contain high levels of fish meal and oil, which are mostly imported, to produce a protein-rich and sometimes lipid-rich (e.g. salmon diets) aquatic feed.
- protein-rich and sometimes lipid-rich e.g. salmon diets
- fish meal and oil can be very expensive and this will be especially true in the future due to progressively increasing demands that are being placed on the finite global supplies offish meal and oil.
- alternative economical sources of protein and lipid are required.
- One known approach is to use less expensive plant protein sources in aquafeed that have been specially processed so that they are in the form of nutritionally upgraded protein meals, concentrates, and isolates. These may be used either singly or in combination with rendered animal protein ingredients such as poultry-by-product meal.
- each of these protein products such as canola meal, soybean meal, and poultry-by-product meal have been processed (produced) separately and then these protein sources have been blended together in dried and finely ground form in appropriate ratios for a particular aquatic species at the time of diet formulation and preparation.
- U.S. Patent No. 4,418,086 to -Marino et al. discloses the preparation of an animal feed which comprises (a) a proteinaceous matrix, (b) fat or oil, (c) a sulfur source, (d) farinaceous material, (e) a plasticizer and (f) water.
- the method disclosed involves the blending of the ingredients together, introducing the mixture into an extruder and subjecting it to shear forces, mechanical work, heat and pressure such that the product temperature prior to discharge is at least 280 degrees F.
- This patent is concerned with the production of an animal feed with a "meat like texture".
- U.S. Patent No. 3,952,115 to Damico et al. relates to a feed where an amino acid is utilized as an additive to fortify a proteinaceous feed.
- U.S. Patent No.4,973,490 to Holmes discloses the production of animal feed products utilizing rape seed in combination with another plant species.
- U.S. Patent No. 5,773,051 to Kim relates to a process for manufacturing a fish feed which refloats after initially sinking. This document discloses a process including blending conventional fish feed containing fish meal, wheat meal, soybean meal and other substances and compressing the mixture at a constant temperature to produce a molded product. Summary of the Invention
- a first aspect involving the preparation of nutritionally upgraded oilseed meals, which are protein and lipid-rich and have a reduced fibre content, and plant oils from oilseeds for use in fish or other non-human animal diets or human foods.
- This process comprises the steps of: - providing a source of oilseed;
- a process for preparation of nutritionally upgraded oilseed meals which are protein and lipid-rich and have a reduced fibre content, and plant oils from oilseeds for use in fish or other non-human animal diets or human foods comprising the steps of:
- the above-described second aspect can be modified as described herein to provide the third process aspect.
- the modifications involve the preparation of protein concentrates and lipid sources from co-processing of animal offal with oilseed for use in fish or other non-human animal feeds, wherein the cold pressing step of said meat fraction or said mixture obtained from the first aspect above is carried out so as to substantially reduce the particle size of said meat or said mixture and to yield a high value human grade oil and a protein and lipid-rich meal with reduced fibre content.
- the third aspect of the process comprises the further steps of:
- a fourth aspect of the process of the present invention involves the preparation of protein concentrates and lipid sources from the co-processing of animal offal with raw oilseeds for use in fish or other non-human animal diets.
- the fourth process aspect comprises the steps of:
- a process for preparation of protein concentrates and lipid sources from the co-processing of animal offal with dried and then dehulled oilseeds for use in fish or other non-human animal diets comprises the steps of:
- a process for producing a protein concentrate for use in animal and aquafeeds comprising:
- the process may further include the step of extracting said protein and lipid-rich meals with a solvent.
- the protein rich fraction of the second to fifth aspects of the process may also be subjected to a solvent extraction to obtain a protein concentrate.
- the step of stabilizing said plant oils by adding an antioxidant there may also be included the step of cooking said mixture to obtain a cooked mixture prior to said extracting step.
- the heat treatment is a rapid heat treatment.
- the heat treatment may be carried out in one or more stages - for example, a two stage heat treatment can be employed where temperatures range from about 100°C to 115°C, and for treatment times ranging from 1.5 minutes to 30 minutes or more depending on the specific components being treated.
- the oilseed is selected from the group consisting of canola, rape seed, soybeans, sunflower seed, flax seed, mustard seed, cotton seed, hemp and mixtures thereof.
- the oilseed may be selected from the group consisting of canola, rape seed, sunflower seed, flax seed, mustard seed, cotton seed and mixtures thereof.
- the oilseed may also be a commercially available processed ground oilseed meal. In this case, the initial steps involving rapid heat-treatment and cold pressing are deleted.
- the oilseed is sunflower.
- oilseed is selected from the group consisting of canola, soybeans, cotton seed, sunflower, hemp and mixtures thereof.
- the animal offal may be selected from the group consisting offish processing waste, whole fish, fish by-catch, squid offal, whole birds, beef offal, lamb offal and mixtures thereof.
- the animal offal is a fish product or poultry.
- squid offal, poultry offal without feet, and whole birds including chickens, turkeys and others without feathers can be used.
- the fish offal or whole fish utilized include fish species having low levels of chlorinated hydrocarbons and heavy metals such as mercury.
- preferred animal offal is a minced unhydrolyzed animal offal.
- the process may include the step of dehulling the heat-treated seed and the blending step may include adding hot water to the mixture.
- the dehulling step in the first, third and fifth process aspects may be carried out by a mechanical treatment with a gravity screening or air-classification step and may also further include a seed sizing step.
- the oilseed can be treated by suitable techniques to remove the outer mucilage layer of the seed coat before the seed is used; the preferred oilseed used in this embodiment includes flax seed.
- oilseed selected from canola, soybeans, sunflower seed, hemp or delinted cotton seed or mixtures thereof is used, due to their global availability, cost, and/or high quality of protein and/or lipid.
- the cooking step may be performed at a temperature of from about 90°C to about 93°C and may further include the step of adding an antioxidant and/or a palatability enhancer to the cooked mixture.
- the antioxidant may be selected from the group consisting of ethoxyquin (santoquin), butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), tertiary butyl hydroquinone, natural antioxidants and mixtures thereof.
- BHA butylated hydroxyanisole
- BHT butylated hydroxytoluene
- tertiary butyl hydroquinone natural antioxidants and mixtures thereof.
- One or more of the foregoing antioxidants are also added to the dried protein concentrate, and the animal feed grade lipid fraction.
- the amount of antioxidant utilized is from about 200 ppm to about 250 ppm whereas the latter is supplemented with about 250 ppm to about 500 ppm antioxidant(s).
- combinations of BHA and ethoxyquin or ethyoxquin alone at highest level is used.
- the palatability enhancer may be.selected from the group consisting of natural and synthetic products based on krill, euphausiids and derivatives thereof, squid, FinnstimTM and mixtures thereof.
- other ingredients such as enzymes, fillers, as well as other sources of lipid of plant or animal origin and other protein sources such as heat-treated field peas or lupins may be added to the composition of the mixture.
- the oilseed and the animal offal in ' the second to fifth process aspects are mixed together in a ratio of about 10:90 to about 90: 10 by weight.
- Preferred ratios in these aspects, as well as in the sixth process aspect is from about 25:75 to about 75:25 by weight or from about 60:40 to about 40:60 by weight.
- the amount of oilseed present in the mixture depends upon the sources of oilseed and animal offal actually used. This amount also depends on respective attendant concentrations of protein and lipid, as well as costs.
- the oilseed is present in a range of about 5% to about 78% by weight. In preferred embodiments, the oilseed is present in the amount of about 22% to about 78% by weight, and in more preferred embodiments, the range is of about 40% to about 60% by weight.
- the mixture is further pressed and/or centrifuged using respectively either a screw press equipped with perforated screens, an expeller equipped with flat steel bars set edgewise around the periphery and spaced to allow the fluids to flow between the bars, a decanter centrifuge or any combination of these.
- a screw press equipped with perforated screens
- an expeller equipped with flat steel bars set edgewise around the periphery and spaced to allow the fluids to flow between the bars
- a decanter centrifuge or any combination of these.
- fluids generally comprised of water that contains some soluble protein and water soluble antinutritional factors stemming from the oilseed such as glucosinolates, phenolic compounds and unwanted sugars including oligosaccharides (raffinose and stachyose).
- Animal feed grade plant oil that is enriched with fatty acids from the animal offal lipid is also removed.
- the drying step in the second to sixth process aspects may be performed at a temperature of between about 70° C to about 85° C.
- the separation step may be carried out in a screw press, expeller press or decanter centrifuge, or any combination thereof.
- the stickwater fraction obtained after separation may be further condensed to yield condensed solubles.
- Preferred embodiments in these process aspects further comprise, if desired, the step of stabilizing said condensed solubles with an inorganic acid.
- the step of incubating said mixture in the presence of one or more enzymes prior to the cooking step may further be included.
- Preferred enzymes used in this embodiment include the enzyme phytase.
- a palatability enhancer When a palatability enhancer is utilized, it may be selected from conventional products based on krill, euphausiids, and/or squid or other like palatability enhancers such as FinnstimTM or the like.
- the palatabiity enhancers may be added to the dried protein concentrates in amounts ranging from about 1 % to about 3% by weight.
- the cooking step in the second to sixth process aspects is carried out using a heat exchanger or through direct steam injection coupled with batch processor.
- the process may further comprise, if desired, the initial step of deboning said animal offal to produced deboned animal offal and bones.
- the cold pressing step in any of the first, third, fifth or sixth process aspects should be carried out at a temperature not exceeding 85°C, desirably below about 70°C.
- the source of the oil seed utilized is most desirably a commercially available particulate processed oil seed meal, which has not been previously subjected to initial rapid heat treatment or cold processing.
- the extraction step in the second to sixth process aspects may be carried out at least twice; preferably the solvent used is or includes hexane.
- An optional feature of various processes described above which involve processing of oilseed prior to co-processing it with animal offal, can utilize the addition of hot water (from about 37°C to about 55°C) to ground oilseed, followed by adjustment of the pH to a value of from about 5.5 to about 6.0 using an inorganic acid such as sulphuric acid; this treatment being carried out in the presence of an enzyme such as the enzyme phytase.
- the various processes of the present invention can be economically and readily carried out using conventional equipment. Such processes will provide cost effective products which can be used in place of or added to other known products in order to achieve additional sources of the desired ingredients for use in fish or other non-human animal diets or human foods.
- the use of inexpensive fish wastes and other animal offal in the various processes of the present invention is a positive way to deal with waste streams rather than considering them as a liability.
- the first product aspect relates to a protein source having from about 40% to about 80% protein, desirably from about 55% to about 77% protein calculated on a lipid-free dry weight basis, said source being adapted for use in animal and aquafeeds and comprising an admixture of treated oilseed protein and animal offal whereby said admixture is characterized by at least one of the following:
- the first product aspect of the invention may also have a reduced content of heat-labile and antinutritional components of at least 80% calculated on a lipid-free dry weight basis.
- This product may further comprise if desired, an antioxidant which may be selected from the group consisting of ethoxyquin (santoquin), butylated hydroxyanisole, butylated hydroxytoluene, tertiary butyl hydroxyquinone, natural antioxidants and mixtures thereof.
- the amount of antioxidant utilized will range depending on the components; generally speaking, this will be from about 200 ppm to about 250 ppm in the protein concentrate, and the animal feed grade lipid fraction resulting from the production of the concentrate may be supplemented with about 250 ppm to about 500 ppm antioxidants. In preferred embodiments, a combination of BHA and ethoxyquin or ethoxyquin alone at highest levels is used.
- the above product invention also comprises enrichment of at least one amino acid selected from the group consisting of arginine, histidine, isoleucine, leucine, lysine, methionine, cystine, phenylalanine, tyrosine, threonine, tryptophan, and valine.
- Preferred amino acids altered in this product are selected from lysine, methionine or cystine.
- This product also comprises enrichment of at least one mineral selected from the group consisting of calcium, phosphorus, magnesium, sodium, potassium, copper and zinc.
- Preferred minerals altered in this product are selected from calcium, phosphorus, sodium, zinc or mixtures thereof.
- This first product comprises enrichment of at least one n-3 highly saturated fatty acid; this is preferably at least one fatty acid selected from eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3) if said source of animal offal is fish.
- the heat-labile and water soluble antinutritional components in the first product are most desirably selected from glucosinates, phenolic compounds including sinapine, chlorogenic acid, oligosaccharides, trypsin inhibitor, saponins and isoflavones or mixtures thereof.
- the digestibility of the first product of the invention is about at least 89% for Atlantic salmon in sea water (fecal settling columns or the Guelph System of fecal collection was used). This percentage may vary and is desirably as high as possible, e.g., in the order of from about 92% to about 100%.
- oilseed in the first product of the invention is selected from the group consisting of canola, rapeseed, soybeans, sunflower seed, flax seed, mustard seed, cotton seed, hemp and mixtures thereof.
- the oilseed may be heat-treated.
- the animal offal in the first product is most desirably selected from the group consisting of whole fish, fish by-catch, fish processing waste, squid offal, whole birds, beef offal, lamb offal and mixtures thereof.
- the protein and lipid contents of the first product are present in an amount within the range (respectively) of about 50% to about 77% calculated on a lipid-free dry weight basis and less than about 10% by weight if the step involving organic solvent extraction has been employed.
- phytate-reduced protein concentrates can be produced.
- the process involves an additional step consisting of adding hot water (temperature of about 37°C to about 55°C) to the ground oilseed in the presence of the enzyme phytase.
- hot water temperature of about 37°C to about 55°C
- the moisture content of the ground oilseed should be raised to about 80% or more and the pH should be about 5.5 to about 6.0 by addition of an inorganic acid, such as sulphuric acid.
- the mixture is then incubated for about 30 minutes and not more than 240 minutes, before being mixed with the animal offal.
- the protein source of this first product finds particular use for animal and fish feeds to cost effectively and extensively replace high nutritive value protein sources such as premium quality fish meal, or conventionally processed oilseed meals that have lower nutritional value.
- the advantage of the above products according to the present invention is that they may be produced in a very economical manner by co-processing sources of protein that heretofore have been processed separately without the attendant benefits of enhancing the nutritive value of the oilseed protein fraction through protein and mineral complementation from the animal offal and by concurrent reduction of the concentrations of heat-labile and water soluble antinutritional factors as well as phytic acid if the optional initial step of phytase pretreatment of the oilseed is adopted.
- These protein products provide significant advantages to animal and fish feed manufacturers which in addition to the economic savings, also provide highly desirable and digestible proteins that have excellent amino acid profiles relative to the essential amino acid needs of commercially important animals and fish.
- a second aspect of the invention relates to another product which is an edible organic oil comprising an oilseed oil, said organic oil having been obtained by cold pressing oilseed in which the cold pressing was carried out at temperatures below 85 ° C, said oil having minimal lipid oxidation products and a peroxide value of less than about 2 milliequivalents per kg following oilseed processing.
- the oilseed providing the oil of the second product is preferably selected from canola, rape seed, sunflower* seed, flax seed, mustard seed, cotton seed and mixtures thereof. In more preferred embodiments, the oilseed is heat-treated.
- the edible organic oils of this aspect of the present invention provide highly nutritional products which can be used for human consumption. Such oils may be packaged and distributed per se or may be incorporated into various types of foods or food compositions where edible oils are required or utilized.
- a further advantage of such organic oils is that they have not been subjected to any organic solvent or other processing steps that would reduce their concentrations of natural anti ⁇ xidarits. Moreover, they are generated under conditions that minimize lipid peroxidation and the products that result from the process. They are highly desired by health conscious people who are concerned with ingesting vegetable oils close to their natural state. Hence, these oils command a premium price in the market place.
- a third product ' aspect relates to an animal feed grade oil for use in animal and aquafeeds comprising an admixture of treated oilseed oil and animal offal, said admixture having an enriched n-3 highly unsaturated fatty acid content (20:5n-3 + 22:6n-3) relative to non-treated oilseed oil if the animal offal used is fish or poultry that have been fed diets comprising adequate concentrations of one or more fish products.
- the oilseed oil of the third product is desirably oil derived from seeds selected from canola, soybeans, sunflower seed, flax seed, hemp and mixtures thereof.
- the oil utilized may be derived from oilseed that has been heat-treated.
- Preferred oilseeds in this embodiment is oil derived from canola seed since the product may further comprise an enriched monounsaturated fatty acid content (18:1 n-9) relative to non-treated oilseed oil.
- the feed grade oils of this aspect of the present invention will find utility in animal and fish foods; they have the advantage that they can be produced in a very efficient and economic manner and they provide highly nutritional sources of enriched unsaturated fatty acid contents.
- the latter lipid sources are highly desirable particularly for use in fish feeds to partially replace premium quality fish oil that may be expensive and difficult to obtain. This is specially true if the plant oil fraction has been enriched with n-3 highly unsaturated fatty acids from the fish offal fraction.
- These oils can be utilized individually or, if desired, combined with other known and conventional oils at the time of feed manufacture.
- a fourth product aspect relates to a constituent for an organic fertilizer comprising at least one of canola, sunflower, soybean, mustard seed, cotton seed and hemp hulls, said hulls being dried hulls and containing protein and lipid.
- the hulls are heat-treated hulls.
- the fourth product can be used in combination with other conventional fertilizer components such as sawdust.
- fertilizers have the advantage of a readily available source of nitrogen.
- the hulls will act as soil conditioner and carrier for nutrients, these being delivered to the soil on a sustained basis.
- the fourth product of the invention will facilitate soil irrigation and water retention in soils. This feature is particularly important in times of drought.
- a fifth product aspect relates to a composition of condensed solubles for use as constituents in organic fertilizers comprising an admixture of treated oilseed and animal offal whereby said admixture has an enriched soluble nitrogen content, water soluble carbohydrate content, water soluble antinutritional component content and mineral content.
- the original hull fraction may be directed for use in ruminant diets either as is or pretreated with carbohydrases.
- the original hull fraction is used in the production of organic fertilizers where it serves as a carrier medium that is completely broken down enzymatically during aerobic or anaerobic decomposition processes.
- the oilseed in the fifth product is selected from canola, rape seed, soybeans, sunflower seed, flax seed, mustard seed, cotton seed, hemp and mixtures thereof.
- the oilseed may be heat-treated.
- the animal offal in the fifth product of the invention is selected from fish processing waste, whole fish, fish by-catch, squid offal, whole birds, beef offal, lamb offal and mixtures thereof.
- the water soluble antinutritional component in the fifth product is selected from glucosinates, phenolic compounds including sinapine, chlorogenicacid, oligosaccharides, saponins or isoflavones.
- the soluble carbohydrate is selected from rrionosaccharides, disaccharides and oligosaccharides.
- the mineral in the fifth product is selected from calcium, phosphorus, magnesium, sodium, potassium, copper, iron and zinc.
- This fifth product is enriched with soluble nitrogen, phosphorus, potassium, as well as organic nutrients. As a constituent for organic fertilizers this product contributes to upgrade the quality of the fertilizer. It should stimulate plant growth, specially the root structure of plants.
- the condensed soluble products of the fifth aspect of the present invention may be utilized with other fertilizer components to provide enhanced fertilizers.
- the condensed solubles can be incorporated into known fertilizers or, if desired, could be marketed as additives per se to known fertilizers.
- a sixth product which relates to a protein and lipid-rich oilseed meal suitable for use in fish and non-human animal diets.
- This product comprises a heat-treated dehulled oilseed, said oilseed being substantially free of flaxseed, mustard seed, rapeseed and cotton seed, said meal having:
- the sixth product of the present invention may further comprise at least one of trypsin inhibitor, glucosinolates, sinapine, chlorogenic acid and mixtures thereof.
- the trypsin inhibitor is in an amount of up to about 8000 units/g on a lipid-free dry weight basis
- the glucosinolates are in an amount of up to about 20 ⁇ moles/g of total glucosinolates on a lipid-free dry weight basis
- the sinapine is in an amount of up to about 2.1 % on a lipid-free dry weight basis
- the chlorogenic acid is in an amount of up to about 3 % on a lipid-free dry weight basis.
- the oilseed in this sixth product may be partially or totally dehulled.
- the protein and lipid rich meals of the sixth product of the present invention can be produced in a very economical manner and will find utility in fish and animal feeds requiring high_ protein and lipid rich meal with reduced concentrations of fibre and heat-labile antinutritional factors. . Their utility will depend on various factors such as the species of animal or fish and their respective requirements for protein and energy, etc. As described previously with respect to other animal and fish feed sources, the products of this aspect of the invention can be incorporated into the feeds of animal and fish as replacements for conventionally processed oilseed meals and oils, and fish meals and oils. Due to the protein and lipid rich content of such products, a beneficial result will be obtained in the increased digestible energy content of diets for such animals and fish.
- the protein concentration can also be increased in the preceding meals through removal of lipid by solvent extraction which increases their utility as components in low energy diets for animals and fish.
- a protein concentrate containing an admixture of a co-processed oilseed and unhydrolyzed animal offal, said concentrate being suitable for use in fish and non-human animal diets, said oilseed comprising a heat-treated dehulled oilseed substantially free of flaxseed, mustard seed, rapeseed and cotton seed, said protein concentrate having:
- the seventh product may further comprise at least one of trypsin inhibitor, glucosinolates, sinapine, chlorogenic acid and mixtures thereof.
- the trypsin inhibitor is in an amount of up to about 2500 units/g on a lipid-free dry weight basis;
- the glucosinolates are in an amount of up to 4.0 ⁇ moles/g of total glucosinolates on a lipid-free dry weight basis;
- sinapine is in an amount of up to about 1.2% on a lipid-free dry weight basis;
- the chlorogenic acid is in an amount of up to about 1.7% on a lipid-free dry weight basis.
- oilseed in this seventh product may be partially or totally dehulled if desired.
- the high digestible protein content, moderate content of highly digestible lipid, reduced fibre content and substantially reduced heat-label and water soluble antinutritional factor content of the seventh product make them suitable as major replacements for fish meal and other conventional sources of protein used in fish and non-human animal diets.
- Their enriched content of at least some of the essential amino acids and minerals, together with their economical cost of production will make such products highly desirable as feed stuff commodities throughout the world.
- an animal feed grade oil comprising oil derived from an admixture of a co- processed oilseed and unhydrolyzed animal offal, said oil being substantially free of flaxseed oil, mustard seed oil, rapeseed oil and cotton seed oil, said animal feed grade oil having:
- the oilseed in this eighth product may be a raw oilseed or a heat-treated oilseed.
- the animal offal is a fish product and the product further comprises (20:5n-3+22:6n-3).
- the eighth product has a generally high content of n-3 highly unsaturated fatty acids compared to the oil from the initial oilseed used if the source of animal offal is fish and hence it is desirable for use in both fish and animal diets.
- the additional benefits of this type of product include ease of production, economical attributes, readily available sources of natural products for obtaining the oil, and its adaptability to incorporation into existing animal diets, as well as its utility as a separate dietary component.
- a ninth product aspect of the present invention relates to an edible organic oil comprising oil of cold pressed heat-treated oilseed, said oil being substantially free of flaxseed oil, mustard seed oil, rapeseed oil and cotton seed oil, said organic oil comprising:
- the ninth product may further comprise up to about 22% of total fatty acids as (n-3) fatty acids.
- the oilseed in the ninth product may be undehulled, partially dehulled or totally dehulled if desired.
- This ninth product is a very cost-effective organic oil for the increasing organic human food industry. As described above with respect to the second product embodiment of this invention, the oil of this embodiment will find utility in various types of food products or as a separate product in and of itself.
- particularly preferred embodiments are those where the animal feed grade oil is an oil derived from raw oilseed; likewise, in another embodiment, the edible organic oils may be derived from raw oilseed.
- the solvent used for extracting the mixture obtained from co-processing of oilseed and animal offal includes hexane or other compatible solvents used in the animal feed or human food industry.
- the ash content in the protein concentrates can be regulated as desired by controlling the concentration of bone in the animal offal.
- the ash can be controlled by using a deboning step to obtain offal with the desired bone content.
- Bones in wet or dry form of different types of offal can be utilized, with varying degrees of bone coarseness.
- the ash content can thus be controlled by controlling the amount of bone added to the mixture of oilseed and animal offal.
- dehulled when using dehulled seeds, the term "dehulled" is intended to mean seeds which have substantially all of their hulls removed. However, in many cases, partially dehulled seeds can be employed as otherwise noted herein, and to this end, dehulled seeds are those which have had at least 55% of their hulls removed.
- the term "unhydrolyzed" in describing the animal offal refers to animal offal which has less than about 20% by weight of hydrolyzed content, desirably less than 5% and most desirably no hydrolysis whatsoever (fresh, unspoiled). In most preferred embodiments, the amount of hydrolyzed content is as close as possible to 0% in order to best achieve the highest nutritive value in the products that are formed.
- the animal offal is preferably in a particulate form such as that which would be obtained by processing procedures resulting in minced offal.
- Well known techniques in the offal processing art can be employed to obtain such minced offal.
- Figure 1 is a schematic representation of the process according to the present invention.
- Figure 1 a schematic representation of the co-processing of animal offal(s) with oilseed(s) to yield cold pressed oil indicated as product 1; hulls from dehulled oilseed meats indicated as product 2; nutritionally upgraded oilseed meal produced from heat treated, dehulled and cold pressed oilseed indicated as product 3; animal- feed grade oil indicated as product 4; condensed solubles indicated as product 5; and high nutritive value protein concentrate indicated as product 6.
- Other products of the invention are obtained by further processing the above-mentioned products as will be described in greater detail hereinafter.
- undehulled oilseed (A) is used in the process.
- Other embodiments involve dehulled seed (B) and raw seed.
- Dehulled seed is preferred when it is desired to feed monogastric species such as fish and poultry, and the preferred raw seed used in this embodiment includes canola, sunflower, or delinted cottonseed.
- Fresh poultry offal (heads and viscera minus feet) was also used for some trials that involved co-processing the offal with partially dehulled animal feed grade sunflower seed (designated as batch 2 hereinafter).
- the offal was obtained from West Coast Reduction Ltd., Vancouver, BC and was stored for one night at -20°C under cover before being handled as described above for the herring.
- oilseeds The four oilseeds that have been tested successfully in this project include Goliath canola seed (Cloutier Agra Seeds Inc., Winnipeg, MB), soybeans (InfraReady Products Ltd. , Saskatoon SK), sunflower (completely dehulled confectionary grade seed obtained from North West Grain, St. Hilaire, MN, USA (batch 1) and undehulled animal feed grade seed obtained from Cargill Incorporated, Wayzata, MN, USA; batch 2), and devitalized hemp seed (Seedtec/Terramax, Qu'Appelle, SK sterilized by InfraReady Products Ltd., Saskatoon SK).
- Goliath canola seed (Cloutier Agra Seeds Inc., Winnipeg, MB)
- soybeans InfraReady Products Ltd. , Saskatoon SK
- sunflower completely dehulled confectionary grade seed obtained from North West Grain, St. Hilaire, MN, USA (batch 1) and undehulle
- an initial heat treatment was performed.
- the process involved subjecting the whole seeds to infrared energy so that the seed temperature reached 110-115°C for 90 seconds. Subsequently, the micronized seeds were held for 20-30 min, depending upon the seed source, in an insulated tank where temperatures ranged from 100-110°C (residual cooking conditions).
- inactivated enzymes such as myrosinase in canola and trypsin inhibitors in soya as well as peroxidase and cyanogenic glucosides. Further, they ensured devitalization of viable germ tissue in hemp, improved starch digestibility, and destroyed or reduced the concentrations of heat labile antinutritional factors other than those mentioned above.
- Sunflower seeds (batches 1 and 2) were not micronized before co-processing with animal offal but the batch 1 seeds were dried to £ 10% moisture to ensure proper seed storage and facilitate dehulling. Thus, only non-micronized dehulled sunflower seeds were tested in this study.
- Micronized canola, soya, hemp and flax and non-micronized animal feed-grade sunflower were dehulled.
- the process involved seed sizing, impact dehulling (Forsberg model 15-D impact huller), screening and air classification (Forsberg model 4800-18 screener and screen-aire).
- the oilseeds (micronized or raw), except soya and micronized dehulled hemp were cold-pressed at a temperature not exceeding 85°C, using a Canadian designed and manufactured laboratory scale Gusta cold press (1 HP Model 11 , Gusta Cold Press, St. Andrews, Manitoba, Canada). This served to remove some (dehulled seeds) or a significant proportion (undehulled seeds) of the residual oil (organic human food grade oil) and concomitantly reduced the particle size of the oilseed before it was co-processed with minced animal offal in various proportions (improved the efficiency of the subsequent aqueous extraction of the water soluble antinutritional factors and oligosaccharides present in the oilseed).
- the particle size was further reduced, using a modified crumbier (model 706S, W.W. Grinder Corp., Wichita, Kansas). This machine was equipped after modification with dual motorized corrugated rolls. One of these had a fixed speed whereas the speed of the other could be varied. For the purpose of this investigation, the variable speed roller was adjusted to rotate much faster than the fixed speed roller to achieve a shearing action.
- oilseeds that had been micronized or dried as described in Example 3 or in raw form and either cold pressed or ground as described in Example 5 were first combined in various proportions.
- the usual percentages of offal to oilseed were 75:25; 50:50; or 25:75 (w/w).
- the mixture obtained from co-processing of animal offal and oilseed was cooked for about 27 min at 90-93°C in the steam jacketed cooker section of a pilot-scale fish meal machine (Chemical Research Organization, Esbjerg, Denmark), that was equipped with a heated auger (it is notworthy that the cooking step could have also been performed by using a heat exchanger with a positive displacement pump or through direct steam injection coupled with processor).
- the cooking step was undertaken to: (1 ) minimize the loss of soluble protein through protein denaturation, (2) destroy or reduce the concentration of heat labile antinutritional factors present in the oilseed (especially important when processing non-micronized seeds and micronized soya), (3) liberate the bound cellular water and lipid in the offal and the oilseed, and (4) subject the oilseed to aqueous washing to facilitate removal of the water soluble antinutritional factors originating from this source.
- EXAMPLE 9 Drying step In one preferred embodiment, further drying of the protein products was necessary to reduce their moisture content. The drying was performed for about 30 min to reduce their moisture content to less than 10%. This was accomplished using a custom designed vertical stack (stainless steel mesh trays) pellet cooler that was equipped with two electric base heaters and a top mounted variable speed fan. The temperature of the upward drawn air was maintained between 70°C and 80°C during the process. All protein and lipid sources stemming from the above process, including the cold-pressed oils were further stabilized with santoquin (ethoxyquin). In a more preferred embodiment, specially in the case of the dried protein products, 100 mg of santoquin were added per kg of product in a marine oil carrier (1 g/kg).
- each of the products was vacuum packaged in oxygen impermeable bags and stored at -20°C pending chemical analysis or their evaluation in a digestibility trial (see below).
- 500 mg of santoquin were added per kg and then each lipid source was stored at -5°C in 1 L black plastic bottles.
- the press liquid was separated into water and lipid fractions using an Alpha de Laval batch dairy centrifuge (Centrifuges Unlimited Inc., Calgary, Alberta). Then, the water fraction was condensed to about one third of its original volume using a steam jacketed bowl cooker.
- EXAMPLE 11 Preparation of protein concentrates Protein concentrates that are mostly based on protein from canola, soya, sunflower and hemp were prepared by hexane extracting the products that originated from the coprocessing of 1 :1 combinations of whole herring and each of the preceding oilseeds.
- 200 g of each of the four protein products were extracted four times with hexane (5: 1 v/w). During each extraction the mixture was held for 30 min (stirred once after 15 min) before being filtered through Whatman No.1 filter paper in a Buchner funnel. Following hexane extraction, each protein product was placed on a tray that was lined with aluminum foil and then it was air-dried overnight. Then, each product was placed in the pellet cooler described in Example 9, where it was dried at about 70-80°C for 15 min to remove any residual traces of hexane.
- the in vivo availability (digestibility) of protein in some of the test protein sources that were prepared by co-processing various proportions of whole herring with canola, soya, sunflower and hemp was determined using Atlantic salmon in sea water as the test animal. Two experiments were conducted and the experimental conditions for each are provided in the table 1 below, wherein the flow rate of the oxygenated, filtered, ambient sea water was 6 - 8 L/min, feeding frequency was twice daily, ration was maximum (fish fed to satiation), and the photoperiod was natural. Table 1.
- Menhaden oil stabilized 3/ 122.4
- Vitamin C monophosphate (42%) 3.38
- the vitamin supplement provided the following amounts/kg of diet on an air-dry basis: vitamin A acetate, 4731 IU; cholecalciferol (D 3 ), 2271 IU; DL- ⁇ - tocopheryl acetate (E), 284 IU; menadione, 17.0 mg; D-calcium pantothenate, 159.3 mg; pyridoxine HCI, 46.6 mg; riboflavin, 56.8 mg; niacin, 283.8 mg; folicacid, 14.2 mg; thiamine mononitrate, 53.0 mg; biotin, 1.42 mg; cyanocobalamin (B 12 ), 0.085 mg; inositol, 378.5 mg.
- the mineral supplement provided the following (mg/kg diet on an air-dry basis): manganese (as MnS0 4 ⁇ H 2 0), 71.0; zinc (as ZnS0 4 • 7H 2 0), 85.2; cobalt (as CoCI 2 - 6H 2 0), 2.84; copper (as CuS0 4 • 5H 2 0), 6.62; iron (as FeS0 4 • 7H 2 0), 94.6; iodine (as KI0 3 and Kl, 1 : 1 ), 9.46; fluorine (as NaF), 4.73; selenium (as Na 2 Se0 3 ), 0.19; sodium (as NaCI), 1419; magnesium (as MgS0 4 • 7H 2 0), 378; potassium (as K 2 S0 4 and K 2 C0 3 , 1 :1), 1419.
- the reference and experimental diets (mixture of reference and test diet) and lyophilized fecal samples were analyzed for levels of moisture, protein and chromic oxide at the DFO, West Vancouver Laboratory (WVL) using the procedures described below. Subsequently, the digestibility coefficients for protein were determined for each diet according to Cho etal. (1985. Finfish nutrition in Asia: methodological approaches to research and development. IDRC Ottawa, Ont., 154p.). Then, the digestibility coefficients for each of the protein products themselves were calculated according to Forster (1999. Aquaculture Nutrition 5: 143-145).
- Examples 13 to 16 outlined hereinafter give the results of chemical analyses performed on products obtained in accordance with the process of the invention from: canola and canola-based products, sunflower and sunflower-based products, soya and soya-based products, as well as hemp and hemp-based products.
- the chemical analyses were performed according to the following methods:
- Concentrations of protein, moisture, and ash in the protein sources and products that were prepared as well as in all test diets, and fecal samples were determined at the Department of Fisheries and Oceans, West Vancouver Laboratory (DFO-WVL) using the procedures described by Higgs et al. (1979. in_J.E. Halver, and K. Tiews, eds. Finfish Nutrition and Fishfeed Technology, Vol. 2. Heenemann Verlagsgesellschaft MbH., Berlin, pp. 191-218).
- the fatty acid compositions of the cold pressed oils and animal feed grade oils stemming from the press liquids were determined at the same laboratory using the procedures of Silver etal. (1993. In SfJ. Kaushik and P. Luquet, eds. Fish nutrition in practice. IV th International Symposium on Fish Nutrition and Feeding, INRA, Paris, pp. 459-468).
- chromic acid concentrations in diets and lyophilized fecal samples were determined at the DFO-WVL using the methods of Fenton and Fenton (1979. Can. J. Anim. Sci., 59: 631-634).
- glucosinolate compounds Concentrations of glucosinolate compounds (total of all the different types of glucosinolates) present in canola and canola- based products were measured by Dr. Phil Raney, of Agriculture & Agri-Food Canada, Saskatoon, SK according to the methods of Daun and McGregor (1981. Glucosinolate Analysis of Rapeseed (Canola). Method of the Canadian Grain Commission Revised Edn. Grain Research Laboratory, Canadian Grain Commission, Winnipeg, Manitoba, Canada).
- soy isoflavones namely, daidzein, glycitein, genistein, and saponins were conducted by Dr. Chung-Ja C. Jackson, of the Guelph Center for Functional Foods, University of Guelph Laboratory Services and have been reported here as the total for the preceding compounds (the methodology in each case is the subject of a patent application and hence has not been published).
- EXAMPLE 13 Results obtained for canola and canola-based products Table 3 outlined below gives the percentages of extensively dehulled and partially dehulled Goliath canola seed and of hulls in relation to seed size after dehulling by Forsberg Incorporated, Thief River Falls, MN.
- dehulled canola The extensively dehulled canola as identified visually by the lack of hulls in the material was used in the tests reported below (referred to as dehulled canola)
- the partially dehulled canola could be subjected to further dehulling, directed into ruminant diets, and/or mixed at a low proportion with animal offal and then co-processed to create a nutritionally upgraded protein source for monogastrics.
- the hulls contained little visible evidence of canola meats and had low density.
- Table 4 gives the percentages of presscake and oil obtained after cold pressing raw, undehulled and micronized, dehulled Goliath canola seed using a laboratory scale Gusta press. Table 4.
- Table 5 sets out the initial ratios of water from endogenous and exogenous sources to oilseed lipid-free dry matter content and percentage yields (air-dry product, moisture- free product, and lipid-free dry weight product) from the co-processing of different blends of whole herring (WH) with dehulled, micronized (DC) and undehulled raw Goliath canola seed (URC).
- WH whole herring
- DC dehulled, micronized
- URC undehulled raw Goliath canola seed
- Protein product 1 Initial ratio of hot Air-dry Moisture- Lipid-free water to oilseed product free product dry product lipid-free dry (%) (%) (%) (%) matter (w/w)
- WH, DC, and URC refer to initial percentages of these products in the herring/canola seed blends (canola seed was cold pressed to remove a significant portion of the oil and reduce the particle size of the starting material before blending with herring and santoquin; 0.1 g/kg of mixed product before water addition) before their coprocessing using cooking temperatures of 90-93°C and drying temperatures of 77-83°C.
- proximate constituents including crude fibre (CF) as well as phytic acid (PA), total glucosinolates (TG), and sinapine in whole herring (WH), dehulled micronized cold pressed Goliath canola (DC), undehulled raw cold pressed Goliath canola (URC), and six protein products produced by the co-processing of different proportions of WH with either DC or URC (expressed on a dry weight basis, DWB or lipid-free dry weight basis, LFDWB) are provided.
- DC dehulled micronized cold pressed Goliath canola
- URC undehulled raw cold pressed Goliath canola
- LFDWB lipid-free dry weight basis
- composition of a seventh protein product that was produced by hexane extraction of WH50DC50 is also shown (WH50DC50-hexane) together with the apparent protein digestibility coefficients for some of the products (Atlantic salmon in sea water used as the test animal) is also provided.
- Table 7 provides the concentrations of essential amino acids (% of protein) and selected minerals ( ⁇ g/g of lipid-free dry matter) in whole herring (WH), micronized, dehulled, cold pressed Goliath canola (DC), undehulled, raw cold pressed Goliath canola (URC), and six protein products produced by the co-processing of different propotions of WH with either DC or URC.
- the amino acid and mineral concentrations in a seventh protein product, produced by hexane extraction of WH50DC50 are also shown (WH50DC50- hexane).
- Table 8 sets out the percentages of selected fatty acids and of saturated, unsaturated, (n-6), (n-3) and n-3 highly unsaturated fatfy acids (n-3 HUFA; 20:5 (n-3) + 22:6 (n-3)) in whole herring (WH), undehulled raw cold pressed Goliath canola (URC), and the press lipids resulting from th e co-processing of different proportions of WH with DC or URC.
- WH whole herring
- URC undehulled raw cold pressed Goliath canola
- EXAMPLE 14 Results obtained for sunflower and sunflower-based products In Table 9, initial ratios of water from endogenous and exogenous sources to oilseed lipid-free dry matter and percentage yields (air-dry product, moisture-free product, and lipid-free dry weight product) from the co-processing of different blends of whole herring (WH) or poultry offal (PO) with dehulled, raw sunflower seed, batch 1 (DRSF.,) or batch 2 (DRSF 2 ) are provided.
- WH whole herring
- PO poultry offal
- Numbers following WH, DRSF and PO refer to initial percentages of these products in the herring/sunflower seed and poultry/sunflower seed blends (sunflower seed was cold pressed to remove a significant portion of the oil and reduce the particle size of the starting material before blending with herring or poultry and santoquin; 0.1 g/kg of mixed product before water addition) before their co-processing using cooking temperatures of 90-93°C and drying temperatures of 77-83°C.
- Table 10 gives the concentrations of proximate constituents including crude fibre (CF), phytic acid (PA), trypsin inhibitor activity (Tl), urease activity (UA) and chlorogenic acid (CA) content in whole herring (WH), poultry offal (PO), dehulled, raw cold pressed sunflower, batch 1 (DRSF.,), and five protein products produced by the co-processing of different proportions of WH or PO with either DRSF ! or dehulled, raw cold pressed sunflower, batch 2 (DRSF 2 ) (expressed on a dry weight basis, DWB or lipid-free dry weight basis, LFDWB).
- CF crude fibre
- PA phytic acid
- Tl trypsin inhibitor activity
- U urease activity
- CA chlorogenic acid
- composition of a sixth protein product that was produced by hexane extraction of WH50DRSF.,50 is also shown (WH ⁇ ODRSF ⁇ O-hexane) together with the apparent protein digestibility coefficients for some of the products (Atlantic salmon in sea water used as the test animal).
- Table 10 The composition of a sixth protein product that was produced by hexane extraction of WH50DRSF.,50 is also shown (WH ⁇ ODRSF ⁇ O-hexane) together with the apparent protein digestibility coefficients for some of the products (Atlantic salmon in sea water used as the test animal).
- Table 11 gives the concentrations of essential amino acids (% of protein) and selected minerals ( ⁇ g/g of lipid-free dry matter) in whole herring (WH), poultry offal (PO), dehulled, raw, cold pressed sunflower, batch 1 (DRSF.,), and four protein products produced by the co-processing of different proportions of WH or PO with either DRSF ! or DRSF 2 .
- the concentrations in a fifth protein product, produced by hexane extraction of WH50DRSF 1 50, is also shown (WH50DRSF 1 50-hexane).
- WH, DSY and URSY refer to initial percentages of these products in the herring/soya blends (soya seed was ground to reduce the particle size of the starting material before blending with herring and santoquin; 0.1 g/kg of mixed product before water addition) prior to their co-processing using cooking temperatures of 90-93 °C and drying temperatures of 77-83 °C.
- Table 14 shows the concentrations of proximate constituents including crude fibre (CF) as well as phytic acid (PA), total saponins, total isoflavones (TIF), urease activity (UA), and trypsin inhibitor activity (Tl) in whole herring (WH), dehulled, micronized, , soya (DSY), undehulled, raw soya (URSY), and six protein products produced by the coprocessing of different proportions of WH with either DSY or URSY (expressed on a dry weight basis, DWB or lipid-free dry weight basis, LFDWB).
- composition of a seventh protein product that was produced by hexane extraction of WH50DSY50 is also shown (WH50DSY50-hexane) together with the apparent protein digestibility coefficients for some of the products (Atlantic salmon in sea water used as the test animal).
- Table 14 The composition of a seventh protein product that was produced by hexane extraction of WH50DSY50 is also shown (WH50DSY50-hexane) together with the apparent protein digestibility coefficients for some of the products (Atlantic salmon in sea water used as the test animal).
- Table 15 provides concentrations of essential amino acids (% of protein) and selected minerals ( ⁇ g/g of lipid-free dry matter) in whole herring (WH), dehulled, micronized, soya (DSY), and three protein products produced by the co-processing of different proportions of WH with DSY.
- Table 16 provides the percentages of selected fatty acids and of saturated, unsaturated, (n-6), (n-3) and n-3 highly unsaturated fatty acids (n-3 HUFA; 20:5 (n-3) + 22:6 (n-3)) in whole herring (WH), micronized, dehulled, soya (DSY), undehulled, raw soya (URSY), and the press lipids resulting from the co-processing of different proportions of WH with DSY or URSY.
- Example 16 Results obtained for hemp and hemp-based products.
- WH, DHP and UHP refer to initial percentages of these products in the herring/hemp blends (UHP seed was cold pressed to remove a significant portion of the oil and to reduce the particle size of the starting material before blending with herring and santoquin; 0.1 g/kg of mixed product before water addition) prior to their coprocessing using cooking temperatures of 90-93°C and drying temperatures of 77-83 °C.
- Table 18 gives the concentrations of proximate constituents including crude fibre (CF) as well as phytic acid (PA) in whole herring (WH), dehulled, sterilized hemp (DHP), cold pressed undehulled, sterilized hemp (UHP), and six protein products produced by the co-processing of different proportions of WH with either DHP or UHP (expressed on a dry weight basis, DWB or lipid-free dry weight basis, LFDWB).
- the composition of a seventh protein product that was produced by hexane extraction of WH50DHP50 is also shown (WH50DHP50-hexane) together with the apparent protein digestibility coefficients for some of the products (Atlantic salmon in sea water used as the test animal).
- Table 18 gives the concentrations of proximate constituents including crude fibre (CF) as well as phytic acid (PA) in whole herring (WH), dehulled, sterilized hemp (DHP), cold pressed undehulled, sterilized hemp (UHP
- Table 19 shows the concentrations of essential amino acids (% of protein) and selected minerals ( ⁇ g/g of lipid-free dry matter) in whole herring (WH), dehulled, sterilized hemp (DHP), and three protein products produced by the co-processing of different proportions of WH with DHP. or UHP.
- Table 20 sets out the percentages of selected fatty acids and of saturated, unsaturated, (n-6), (n-3) and n-3 highly unsaturated fatty acids (n-3 HUFA; 20:5 (n-3) + 22:6 (n-3)) in whole herring (WH), dehulled, sterilized hemp (DHP), undehulled, sterilized hemp (UHP), and the press lipids resulting from the co-processing of different proportions of WH with DHP or UHP.
- the oilseed-based protein products contained high concentrations of protein that was highly bioavailable to salmon (generally 89% to 100% of the protein was noted to be digestible in Atlantic salmon held " in sea water depending upon the source and percentage of the oilseed in the initial mixture of offal and oilseed and the pretreatment of the latter and the offal before their co-processing). Moreover, these protein products had significantly reduced concentrations of all heat labile and water soluble antinutritional factors except phytic acid relative to their respective initial levels in the oilseeds. Phytic acid was concentrated during the co-processing of offal with oilseed and the extent depended upon its initial " concentration in the oilseed used in the process.
- the fatty acid compositions of the animal feed grade lipid sources produced by the process largely reflected the fatty acid compositions and lipid levels contributed by the different proportions of the animal offal and oilseed used initially in the process. This provides considerable scope to produce specially designed lipid sources that are tailored to meet the fatty acid needs of various animal species.
- oilseeds before they are blended with animal offal yielded high quality economically valuable human food grade oils whose fatty acid compositions can be varied, depending upon market requirements and the selection of the oilseed or combination of oilseeds that are used in cold pressing.
- the high value of the cold pressed oils which can be generated in greater quantities wen undehulled seeds rather than dehulled seeds are cold pressed will contribute to the overall economic viability of the co-processing of animal offals with oilseeds.
- oilseeds to inactivate enzymes like the protease inhibitors in soya and destruct heat labile antinutritional components coupled with the dehulling of oilseeds yield protein and lipid-rich products that potential can be used directly in high energy feeds such as those destined for aquatic species like salmon (salmon grower diets frequently contain 25-35% lipid on an air-dry basis and they are produced by extrusion processing technology).
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Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
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AU5811301A AU5811301A (en) | 2000-05-09 | 2001-05-08 | Protein and lipid sources for use in aquafeeds and animal feeds and a process for their preparation |
EP01931283A EP1280874A2 (en) | 2000-05-09 | 2001-05-08 | Protein and lipid sources for use in aquafeeds and animal feeds and a process for their preparation |
AU2001258113A AU2001258113B2 (en) | 2000-05-09 | 2001-05-08 | Protein and lipid sources for use in aquafeeds and animal feeds and a process for their preparation |
CA002408551A CA2408551C (en) | 2000-05-09 | 2001-05-08 | Protein and lipid sources for use in aquafeeds and animal feeds and a process for their preparation |
US10/076,499 US6955831B2 (en) | 2000-05-09 | 2002-02-19 | Protein and lipid sources for use in aquafeeds and animal feeds and a process for their preparation |
US11/206,143 US20060051489A1 (en) | 2000-05-09 | 2005-08-18 | Protein and lipid sources for use in aquafeeds and animal feeds and a process for their preparation |
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US56672800A | 2000-05-09 | 2000-05-09 | |
US09/566,728 | 2000-05-09 | ||
CA002335745A CA2335745A1 (en) | 2001-02-13 | 2001-02-13 | Process for preparing nutritionally upgraded canola products |
CA2,335,745 | 2001-02-13 |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
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EP1346647A1 (en) * | 2002-03-22 | 2003-09-24 | Peter Brauchl | Fish feed for salmonids |
WO2004112493A1 (en) * | 2003-06-20 | 2004-12-29 | Burcon Nutrascience (Mb) Corp. | Oil seed meal preparation |
JP2007524687A (en) * | 2004-02-17 | 2007-08-30 | バーコン ニュートラサイエンス (エムビー) コーポレイション | Preparation of canola protein isolate and its use in aquaculture |
WO2012016307A1 (en) | 2010-08-02 | 2012-02-09 | Granol Industria Comércio E Exportaçao Sa | "process for purification of vegetable oils upon withdrawal of solids by centri fugat ion in the miscella stage |
WO2012168390A1 (en) * | 2011-06-09 | 2012-12-13 | Erapoil As | Process for the production of plant oil |
US9464309B2 (en) | 2002-07-29 | 2016-10-11 | Zymtech Holding Ag | Methods for recovering peptides/amino acids and oil/fat from one or more protein-containing raw materials, and products produced by the methods |
CN107334678A (en) * | 2017-07-18 | 2017-11-10 | 安徽省华银茶油有限公司 | A kind of method that antibacterial wet tissue is prepared using camellia seed dregs of rice extract |
CN110438198A (en) * | 2019-09-05 | 2019-11-12 | 湖南农业大学 | The ramee variety and organ screening technique of antibiotic are prepared based on grass meal extract |
EP3440943A4 (en) * | 2016-04-04 | 2019-11-20 | Ajinomoto Co., Inc. | Feed for aquatic organisms |
Families Citing this family (2)
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CN102754679B (en) * | 2012-08-02 | 2014-07-30 | 中国水产科学研究院渔业机械仪器研究所 | Method for preparing peeled Antarctic Krill |
CN115181590B (en) * | 2022-07-29 | 2023-06-13 | 重庆科技学院 | Biomass double-circulation gasification decarburization reaction system in graded decoupling mode |
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JPH0662352B2 (en) * | 1987-04-20 | 1994-08-17 | 焼津ミ−ル協業組合 | Method for producing organic fertilizer |
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2001
- 2001-05-08 EP EP01931283A patent/EP1280874A2/en not_active Withdrawn
- 2001-05-08 AU AU2001258113A patent/AU2001258113B2/en not_active Ceased
- 2001-05-08 AU AU5811301A patent/AU5811301A/en active Pending
- 2001-05-08 WO PCT/CA2001/000663 patent/WO2001084949A2/en active IP Right Grant
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DE3927118A1 (en) * | 1989-08-17 | 1991-02-21 | Hungaromix Agrarfejlesztoe Kft | Protein feed concentrate rich in animal and plant aminoacid(s) - opt. enriched with lactic acid, prepd. :from animal waste and legumes, using process heat to destroy trypsin inhibitor |
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Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1346647A1 (en) * | 2002-03-22 | 2003-09-24 | Peter Brauchl | Fish feed for salmonids |
US9464309B2 (en) | 2002-07-29 | 2016-10-11 | Zymtech Holding Ag | Methods for recovering peptides/amino acids and oil/fat from one or more protein-containing raw materials, and products produced by the methods |
US7645468B2 (en) | 2003-06-20 | 2010-01-12 | Burcon Nutrascience (Mb) Corp. | Oil seed meal preparation |
WO2004112493A1 (en) * | 2003-06-20 | 2004-12-29 | Burcon Nutrascience (Mb) Corp. | Oil seed meal preparation |
JP2007508001A (en) * | 2003-06-20 | 2007-04-05 | バーコン ニュートラサイエンス (エムビー) コーポレイション | Oilseed meal preparation |
AU2004248870B2 (en) * | 2003-06-20 | 2010-08-19 | Burcon Nutrascience (Mb) Corp. | Oil seed meal preparation |
CN1835683B (en) * | 2003-06-20 | 2012-06-27 | 伯康营养科学(Mb)公司 | Oil seed meal preparation |
KR101160579B1 (en) * | 2003-06-20 | 2012-06-28 | 버콘 뉴트라사이언스 (엠비) 코포레이션 | Oil seed meal preparation |
JP2007524687A (en) * | 2004-02-17 | 2007-08-30 | バーコン ニュートラサイエンス (エムビー) コーポレイション | Preparation of canola protein isolate and its use in aquaculture |
WO2012016307A1 (en) | 2010-08-02 | 2012-02-09 | Granol Industria Comércio E Exportaçao Sa | "process for purification of vegetable oils upon withdrawal of solids by centri fugat ion in the miscella stage |
EP2600954A1 (en) * | 2010-08-02 | 2013-06-12 | Granol Industria Comércio E Exportaçao SA | "process for purification of vegetable oils upon withdrawal of solids by centri fugat ion in the miscella stage |
EP2600954A4 (en) * | 2010-08-02 | 2014-04-02 | Granol Ind Com E Exportacao Sa | "process for purification of vegetable oils upon withdrawal of solids by centri fugat ion in the miscella stage |
WO2012168390A1 (en) * | 2011-06-09 | 2012-12-13 | Erapoil As | Process for the production of plant oil |
EP3440943A4 (en) * | 2016-04-04 | 2019-11-20 | Ajinomoto Co., Inc. | Feed for aquatic organisms |
CN107334678A (en) * | 2017-07-18 | 2017-11-10 | 安徽省华银茶油有限公司 | A kind of method that antibacterial wet tissue is prepared using camellia seed dregs of rice extract |
CN110438198A (en) * | 2019-09-05 | 2019-11-12 | 湖南农业大学 | The ramee variety and organ screening technique of antibiotic are prepared based on grass meal extract |
CN110438198B (en) * | 2019-09-05 | 2023-07-21 | 湖南农业大学 | Ramie variety and organ screening method for preparing antibiotics based on grass meal extract |
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WO2001084949A3 (en) | 2002-05-02 |
AU5811301A (en) | 2001-11-20 |
AU2001258113B2 (en) | 2006-01-12 |
EP1280874A2 (en) | 2003-02-05 |
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