WO2001079851A8 - NON-INVASIVE PRENATAL DIAGNOSIS USING CD45-ve FETAL CELLS - Google Patents

NON-INVASIVE PRENATAL DIAGNOSIS USING CD45-ve FETAL CELLS

Info

Publication number
WO2001079851A8
WO2001079851A8 PCT/GB2001/001676 GB0101676W WO0179851A8 WO 2001079851 A8 WO2001079851 A8 WO 2001079851A8 GB 0101676 W GB0101676 W GB 0101676W WO 0179851 A8 WO0179851 A8 WO 0179851A8
Authority
WO
WIPO (PCT)
Prior art keywords
cells
fetal
testing
fetal cells
prenatal diagnosis
Prior art date
Application number
PCT/GB2001/001676
Other languages
French (fr)
Other versions
WO2001079851A1 (en
Inventor
Nicholas Maxwell Fisk
Cesare Campagnoli
Phillip Robert Bennett
Irene Anne Graham Roberts
Original Assignee
Imp College Innovations Ltd
Nicholas Maxwell Fisk
Cesare Campagnoli
Phillip Robert Bennett
Irene Anne Graham Roberts
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imp College Innovations Ltd, Nicholas Maxwell Fisk, Cesare Campagnoli, Phillip Robert Bennett, Irene Anne Graham Roberts filed Critical Imp College Innovations Ltd
Priority to AU2001248550A priority Critical patent/AU2001248550A1/en
Publication of WO2001079851A1 publication Critical patent/WO2001079851A1/en
Publication of WO2001079851A8 publication Critical patent/WO2001079851A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4712—Muscle proteins, e.g. myosin, actin, protein
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4742—Keratin; Cytokeratin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/70542—CD106
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70589—CD45
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/745—Assays involving non-enzymic blood coagulation factors
    • G01N2333/755—Factors VIII, e.g. factor VIII C [AHF], factor VIII Ag [VWF]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Rheumatology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

CD45-ve fetal cells without a maternal equivalent can be purified and expanded from maternal blood in the first trimester, thereby providing abundant fetal material for genetic testing by a non-invasive route. Advantageously, the cells are adherent and CD14?-ve, CD34-ve, CD68-ve, vWF-ve¿, myoglobin-ve, collagen-I-ve, cytokeratin?-ve, CD44+ve, CD105+ve, CD106+ve¿, prolyl-4-hydroxylase+ve, fibronectin+ve, α-smooth muscle actin+ve, and laminin+ve. A process for preparing cells is disclosed, comprising the steps of (a) extracting a blood sample from a pregnant female, and (b) enriching nucleated CD45-ve fetal cells therein. A process for prenatal testing of a fetus for a genetic disorder comprises the further step of (c) testing the enriched nucleated CD45-ve fetal cell.
PCT/GB2001/001676 2000-04-13 2001-04-12 NON-INVASIVE PRENATAL DIAGNOSIS USING CD45-ve FETAL CELLS WO2001079851A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001248550A AU2001248550A1 (en) 2000-04-13 2001-04-12 Non-invasive prenatal diagnosis using cd45-ve fetal cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0009179A GB0009179D0 (en) 2000-04-13 2000-04-13 Non-invasive prenatal diagnosis
GB0009179.3 2000-04-13

Publications (2)

Publication Number Publication Date
WO2001079851A1 WO2001079851A1 (en) 2001-10-25
WO2001079851A8 true WO2001079851A8 (en) 2002-01-24

Family

ID=9889886

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/001676 WO2001079851A1 (en) 2000-04-13 2001-04-12 NON-INVASIVE PRENATAL DIAGNOSIS USING CD45-ve FETAL CELLS

Country Status (3)

Country Link
AU (1) AU2001248550A1 (en)
GB (1) GB0009179D0 (en)
WO (1) WO2001079851A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9163282B2 (en) 2010-05-18 2015-10-20 Natera, Inc. Methods for non-invasive prenatal ploidy calling

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2445204C (en) 2002-10-16 2014-08-12 Streck Laboratories, Inc. Method and device for collecting and preserving cells for analysis
GB0509500D0 (en) * 2005-05-10 2005-06-15 Revealcyte Method of fetal cell enrichment
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
AU2009279734A1 (en) 2008-08-04 2010-02-11 Natera, Inc. Methods for allele calling and ploidy calling
DK2857527T3 (en) * 2009-01-07 2019-02-25 Arcedi Biotech Aps Enrichment and identification of fetal cells in maternal blood and ligands for such use
US11634747B2 (en) 2009-01-21 2023-04-25 Streck Llc Preservation of fetal nucleic acids in maternal plasma
DE202010018569U1 (en) 2009-02-18 2017-09-26 Streck Inc. Preservation of cell-free nucleic acids
EP2854056A3 (en) 2009-09-30 2015-06-03 Natera, Inc. Methods for non-invasive pre-natal ploidy calling
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
EP2854058A3 (en) 2010-05-18 2015-10-28 Natera, Inc. Methods for non-invasive pre-natal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
PL2638176T3 (en) * 2010-11-09 2018-01-31 Arcedi Biotech Aps Enrichment and identification of fetal cells in maternal blood and ligands for such use
BR112013016193B1 (en) 2010-12-22 2019-10-22 Natera Inc ex vivo method to determine if an alleged father is the biological father of a unborn baby in a pregnant woman and report
US9956281B2 (en) 2011-05-04 2018-05-01 Streck, Inc. Inactivated virus compositions and methods of preparing such compositions
EP3024323B1 (en) 2013-07-24 2022-10-19 Streck, Inc. Compositions and methods for stabilizing circulating tumor cells
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
EP3561075A1 (en) 2014-04-21 2019-10-30 Natera, Inc. Detecting mutations in tumour biopsies and cell-free samples
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
US20170145475A1 (en) 2015-11-20 2017-05-25 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
WO2018022991A1 (en) 2016-07-29 2018-02-01 Streck, Inc. Suspension composition for hematology analysis control
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US11525159B2 (en) 2018-07-03 2022-12-13 Natera, Inc. Methods for detection of donor-derived cell-free DNA
EP4242652A3 (en) 2019-06-07 2023-12-06 Arcedi Biotech ApS Isolation of fetal cells using facs

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993008269A1 (en) * 1991-10-23 1993-04-29 Cellpro, Incorporated A method for enriching fetal progenitor cells from maternal blood
WO1994025873A1 (en) * 1993-04-23 1994-11-10 Cellpro, Incorporated Methods for enriching fetal progenitor cells from maternal blood
EP0778899A4 (en) * 1994-09-20 2001-08-29 Miltenyi Biotech Inc Enrichment of fetal cells from maternal blood

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9163282B2 (en) 2010-05-18 2015-10-20 Natera, Inc. Methods for non-invasive prenatal ploidy calling

Also Published As

Publication number Publication date
GB0009179D0 (en) 2000-05-31
WO2001079851A1 (en) 2001-10-25
AU2001248550A1 (en) 2001-10-30

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