WO2001079543A3 - Assay for identification of target rna binding compounds - Google Patents

Assay for identification of target rna binding compounds Download PDF

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Publication number
WO2001079543A3
WO2001079543A3 PCT/GB2001/001778 GB0101778W WO0179543A3 WO 2001079543 A3 WO2001079543 A3 WO 2001079543A3 GB 0101778 W GB0101778 W GB 0101778W WO 0179543 A3 WO0179543 A3 WO 0179543A3
Authority
WO
WIPO (PCT)
Prior art keywords
target rna
assay
identification
rna
rna binding
Prior art date
Application number
PCT/GB2001/001778
Other languages
French (fr)
Other versions
WO2001079543A2 (en
Inventor
Alastair Iain Hamilton Murchie
Georg Friedrich Lentzen
Original Assignee
Ribotargets Ltd
Alastair Iain Hamilton Murchie
Georg Friedrich Lentzen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0009772A external-priority patent/GB0009772D0/en
Application filed by Ribotargets Ltd, Alastair Iain Hamilton Murchie, Georg Friedrich Lentzen filed Critical Ribotargets Ltd
Priority to EP01969049A priority Critical patent/EP1343910A2/en
Priority to AU93367/01A priority patent/AU9336701A/en
Publication of WO2001079543A2 publication Critical patent/WO2001079543A2/en
Publication of WO2001079543A3 publication Critical patent/WO2001079543A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for determining whether a test compound binds to a target RNA, the method comprising the steps of: (a) contacting the test compound with the target RNA and a RNA modifying enzyme; and (b) detecting the modification of the target RNA by the enzyme and comparing the amount of modification detected to that of a standard. Inhibition of enzymatic modification of the RNA target indicates that the test compound can bind the target RNA and can thus exhibit antibiotic activity.
PCT/GB2001/001778 2000-04-19 2001-04-19 Assay for identification of target rna binding compounds WO2001079543A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01969049A EP1343910A2 (en) 2000-04-19 2001-04-19 Assay for identification of a test compound
AU93367/01A AU9336701A (en) 2000-04-19 2001-04-19 Assay for identification of a test compound

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US19817900P 2000-04-19 2000-04-19
US60/198,179 2000-04-19
GB0009772A GB0009772D0 (en) 2000-04-19 2000-04-19 Assay
GB0009772.5 2000-04-19

Publications (2)

Publication Number Publication Date
WO2001079543A2 WO2001079543A2 (en) 2001-10-25
WO2001079543A3 true WO2001079543A3 (en) 2003-05-30

Family

ID=26244139

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/001778 WO2001079543A2 (en) 2000-04-19 2001-04-19 Assay for identification of target rna binding compounds

Country Status (4)

Country Link
EP (1) EP1343910A2 (en)
AU (1) AU9336701A (en)
GB (1) GB2364386A (en)
WO (1) WO2001079543A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1263986A4 (en) * 2000-02-25 2005-02-23 Tao Biosciences Llc Platform for the discovery of the bacterial genes involved in rna modification
CN108474042B (en) * 2014-10-17 2022-02-11 弗雷德哈钦森癌症研究中心 Reagent component, kit and method for evaluating helicobacter pylori infection
CN111265441A (en) * 2020-03-13 2020-06-12 佛山市奥姿美生物科技有限公司 Composition capable of enhancing skin night repair and regeneration capacity and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996035811A1 (en) * 1995-05-12 1996-11-14 President And Fellows Of Harvard College Methods and kits for rna binding compounds
WO1997009342A1 (en) * 1995-09-08 1997-03-13 Scriptgen Pharmaceuticals, Inc. Screen for compounds with affinity for rna

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996035811A1 (en) * 1995-05-12 1996-11-14 President And Fellows Of Harvard College Methods and kits for rna binding compounds
WO1997009342A1 (en) * 1995-09-08 1997-03-13 Scriptgen Pharmaceuticals, Inc. Screen for compounds with affinity for rna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CUNDLIFFE E ET AL: "RIBOSE METHYLATION AND RESISTANCE TO THIOSTREPTON", NATURE (LONDON), vol. 278, no. 5707, 1979, pages 859 - 861, XP002197419, ISSN: 0028-0836 *

Also Published As

Publication number Publication date
GB2364386A (en) 2002-01-23
EP1343910A2 (en) 2003-09-17
WO2001079543A2 (en) 2001-10-25
GB0109647D0 (en) 2001-06-13
AU9336701A (en) 2001-10-30

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