WO2001075176A3 - Methods and systems for molecular fingerprinting - Google Patents

Methods and systems for molecular fingerprinting Download PDF

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Publication number
WO2001075176A3
WO2001075176A3 PCT/US2001/010881 US0110881W WO0175176A3 WO 2001075176 A3 WO2001075176 A3 WO 2001075176A3 US 0110881 W US0110881 W US 0110881W WO 0175176 A3 WO0175176 A3 WO 0175176A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
fingerprinting
molecules
systems
methods
Prior art date
Application number
PCT/US2001/010881
Other languages
French (fr)
Other versions
WO2001075176A2 (en
Inventor
Stephen R Quake
Hou-Pu Chou
Original Assignee
California Inst Of Techn
Stephen R Quake
Hou-Pu Chou
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Inst Of Techn, Stephen R Quake, Hou-Pu Chou filed Critical California Inst Of Techn
Priority to AU2001249820A priority Critical patent/AU2001249820A1/en
Publication of WO2001075176A2 publication Critical patent/WO2001075176A2/en
Publication of WO2001075176A3 publication Critical patent/WO2001075176A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention relates in general to a method for molecular fingerprinting. The method can be used for forensic identification (e.g. DNA fingerprinting, especially by VNTR), bacteria typing, and human/animal pathogen diagnosis. More particularly, molecules such as polynucleotides (e.g. DNA) can be assessed or sorted by size in a microfabricated device that analyzes the polynucleotides according to restriction fragment length polymorphism. In a microfabricated device according to the invention, DNA fragments or other molecules can be rapidly and accurately typed using relatively small samples, by measuring for example the signal of an optically-detectable (e.g., fluorescent) reporter associated with the polynucleotide fragments.
PCT/US2001/010881 2000-04-04 2001-04-04 Methods and systems for molecular fingerprinting WO2001075176A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001249820A AU2001249820A1 (en) 2000-04-04 2001-04-04 Methods and systems for molecular fingerprinting

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19442200P 2000-04-04 2000-04-04
US60/194,422 2000-04-04

Publications (2)

Publication Number Publication Date
WO2001075176A2 WO2001075176A2 (en) 2001-10-11
WO2001075176A3 true WO2001075176A3 (en) 2003-08-07

Family

ID=22717542

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/010881 WO2001075176A2 (en) 2000-04-04 2001-04-04 Methods and systems for molecular fingerprinting

Country Status (2)

Country Link
AU (1) AU2001249820A1 (en)
WO (1) WO2001075176A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9365822B2 (en) 1997-12-31 2016-06-14 Xy, Llc System and method for sorting cells

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2822983C (en) 2000-11-29 2017-05-09 Xy, Llc System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
KR100459106B1 (en) * 2002-03-21 2004-12-03 한국해양연구원 Identification of an organism by use of the intron dna sequence of the clock gene as dna fingerprints
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DK2305173T3 (en) 2003-03-28 2016-08-22 Inguran Llc Apparatus and method for providing sorted particles
CN101533005B (en) * 2009-04-14 2012-10-10 北京大学 Microflow distribution device, manufacturing method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995033853A1 (en) * 1993-10-20 1995-12-14 University Of Tennessee Research Corporation Fingerprinting of nucleic acids, products and methods
EP0778351A2 (en) * 1995-11-30 1997-06-11 Hitachi, Ltd. Method of analysis or assay for polynucleotides and analyzer or instrument for polynucleotides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995033853A1 (en) * 1993-10-20 1995-12-14 University Of Tennessee Research Corporation Fingerprinting of nucleic acids, products and methods
EP0778351A2 (en) * 1995-11-30 1997-06-11 Hitachi, Ltd. Method of analysis or assay for polynucleotides and analyzer or instrument for polynucleotides

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHOU H P ET AL: "A microfabricated device for sizing and sorting DNA molecules.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. UNITED STATES 5 JAN 1999, vol. 96, no. 1, 5 January 1999 (1999-01-05), pages 11 - 13, XP002217376, ISSN: 0027-8424 *
JU L Y ET AL: "Application of silver staining to the rapid typing of the polymorphism of HLA-DQ alleles by enzymatic amplification and allele-specific restriction fragment length polymorphism.", ELECTROPHORESIS. GERMANY APR 1991, vol. 12, no. 4, April 1991 (1991-04-01), pages 270 - 273, XP008009406, ISSN: 0173-0835 *
MURRAY V ET AL: "Detection of polymorphisms using thermal cycling with a single oligonucleotide on a DNA sequencing gel", HUMAN MUTATION, vol. 2, 1993, pages 118 - 122, XP008009397 *
ROUHI M: "SIZING, SORTING DNA ONE PIECE AT A TIME", CHEMICAL AND ENGINEERING NEWS, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 77, no. 2, 11 January 1999 (1999-01-11), pages 5 - 6, XP000789114, ISSN: 0009-2347 *
TATARI Z ET AL: "HLA-Cw allele analysis by PCR-restriction fragment length polymorphism: study of known and additional alleles.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. UNITED STATES 12 SEP 1995, vol. 92, no. 19, 12 September 1995 (1995-09-12), pages 8803 - 8807, XP002217375, ISSN: 0027-8424 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9365822B2 (en) 1997-12-31 2016-06-14 Xy, Llc System and method for sorting cells
US9422523B2 (en) 1997-12-31 2016-08-23 Xy, Llc System and method for sorting cells

Also Published As

Publication number Publication date
WO2001075176A2 (en) 2001-10-11
AU2001249820A1 (en) 2001-10-15

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