WO2001075128A3 - Verfahren zur herstellung von leguminosen mit erhöhtem proteingehalt bei verlängerter samenfüllungsdauer - Google Patents

Verfahren zur herstellung von leguminosen mit erhöhtem proteingehalt bei verlängerter samenfüllungsdauer Download PDF

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Publication number
WO2001075128A3
WO2001075128A3 PCT/DE2001/001208 DE0101208W WO0175128A3 WO 2001075128 A3 WO2001075128 A3 WO 2001075128A3 DE 0101208 W DE0101208 W DE 0101208W WO 0175128 A3 WO0175128 A3 WO 0175128A3
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WIPO (PCT)
Prior art keywords
protein content
seed
increased protein
plants
production
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PCT/DE2001/001208
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English (en)
French (fr)
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WO2001075128A2 (de
Inventor
Hans Weber
Isolde Saalbach
Martin Giersberg
Petra Hoffmeister
Original Assignee
Inst Pflanzengenetik & Kultur
Hans Weber
Isolde Saalbach
Martin Giersberg
Petra Hoffmeister
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Inst Pflanzengenetik & Kultur, Hans Weber, Isolde Saalbach, Martin Giersberg, Petra Hoffmeister filed Critical Inst Pflanzengenetik & Kultur
Priority to EP01940107A priority Critical patent/EP1268831A2/de
Priority to AU73820/01A priority patent/AU7382001A/en
Publication of WO2001075128A2 publication Critical patent/WO2001075128A2/de
Publication of WO2001075128A3 publication Critical patent/WO2001075128A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Herstellung von Leguminosen mit erhöhtem Proteingehalt im Samen bei verlängerter Samenfüllungsdauer durch Einbringen von rekombinanten DNA-Molekülen. In die Pflanze werden mittels eines Transformationssystems die rekombinanten DNA-Moleküle eingebracht, wobei sie eine pflanzeneigene DNA-Sequenz umfassen, die in Pflanzen zur Expression gebracht wird und deren Genprodukt im Samen ein Protein mit der enzymatischen Aktivität einer ADP-Glucose Pyrophosphorylase (AGP) und/oder einer plastidären Phosphoglucomutase (pPGM) hemmt sowie ggf. die regulatorische Sequenz eines samenspezifischen Promotors in Leguminosen. Des weiteren wird getrennt mindestens ein Selektionsmarkergen übertragen, welches anschliessend wieder entfernt wird. Es werden die Pflanzen selektiert, die einen erhöhten Proteingehalt und eine längere Samenfüllungsdauer aufweisen.
PCT/DE2001/001208 2000-03-31 2001-03-23 Verfahren zur herstellung von leguminosen mit erhöhtem proteingehalt bei verlängerter samenfüllungsdauer WO2001075128A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01940107A EP1268831A2 (de) 2000-03-31 2001-03-23 Verfahren zur herstellung von leguminosen mit erhöhtem proteingehalt bei verlängerter samenfüllungsdauer
AU73820/01A AU7382001A (en) 2000-03-31 2001-03-23 Method for the production of leguminous plants with increased protein content and longer seed filling duration

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10015989.3 2000-03-31
DE10015989 2000-03-31

Publications (2)

Publication Number Publication Date
WO2001075128A2 WO2001075128A2 (de) 2001-10-11
WO2001075128A3 true WO2001075128A3 (de) 2002-04-04

Family

ID=7637081

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/001208 WO2001075128A2 (de) 2000-03-31 2001-03-23 Verfahren zur herstellung von leguminosen mit erhöhtem proteingehalt bei verlängerter samenfüllungsdauer

Country Status (5)

Country Link
EP (1) EP1268831A2 (de)
AR (1) AR027750A1 (de)
AU (1) AU7382001A (de)
DE (1) DE10115762A1 (de)
WO (1) WO2001075128A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7323560B2 (en) 2000-07-17 2008-01-29 E.I. Du Pont De Nemours And Company Plastidic phosphoglucomutase genes
US7250557B2 (en) 2000-07-17 2007-07-31 E. I. Du Pont De Nemours And Company Plastidic phosphoglucomutase genes

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5457274A (en) * 1992-05-15 1995-10-10 Research Corporation Technologies Pink-flowered, high protein soybean plants and methods for their production
WO1996013595A1 (de) * 1994-10-31 1996-05-09 Hoechst Schering Agrevo Gmbh Verfahren zur veränderung des blühverhaltens bei pflanzen
WO1998001574A1 (en) * 1996-07-09 1998-01-15 Unilever Plc Method for increasing sucrose content of plants
US5773693A (en) * 1993-07-23 1998-06-30 Dnap Plant Technology Corporation Pea ADP-glucose pyrophosphorylase subunit genes and their uses
WO1999015004A1 (en) * 1997-09-19 1999-04-01 Commonwealth Scientific And Industrial Research Organisation Method for altering storage organ composition
WO1999029161A1 (en) * 1997-12-08 1999-06-17 Seminis Vegetable Seeds, Inc. A starchless variety of pisum sativum having elevated levels of sucrose
WO1999036551A1 (en) * 1998-01-15 1999-07-22 E.I. Du Pont De Nemours And Company Plant phosphoglucomutase homologs
WO1999053068A2 (en) * 1998-04-09 1999-10-21 E.I. Du Pont De Nemours And Company Sucrose transporters from plants
WO2000043529A1 (en) * 1999-01-19 2000-07-27 Unilever Plc Method of increasing the water soluble antioxidant content in mechanically harvested peas

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5457274A (en) * 1992-05-15 1995-10-10 Research Corporation Technologies Pink-flowered, high protein soybean plants and methods for their production
US5773693A (en) * 1993-07-23 1998-06-30 Dnap Plant Technology Corporation Pea ADP-glucose pyrophosphorylase subunit genes and their uses
WO1996013595A1 (de) * 1994-10-31 1996-05-09 Hoechst Schering Agrevo Gmbh Verfahren zur veränderung des blühverhaltens bei pflanzen
WO1998001574A1 (en) * 1996-07-09 1998-01-15 Unilever Plc Method for increasing sucrose content of plants
WO1999015004A1 (en) * 1997-09-19 1999-04-01 Commonwealth Scientific And Industrial Research Organisation Method for altering storage organ composition
WO1999029161A1 (en) * 1997-12-08 1999-06-17 Seminis Vegetable Seeds, Inc. A starchless variety of pisum sativum having elevated levels of sucrose
WO1999036551A1 (en) * 1998-01-15 1999-07-22 E.I. Du Pont De Nemours And Company Plant phosphoglucomutase homologs
WO1999053068A2 (en) * 1998-04-09 1999-10-21 E.I. Du Pont De Nemours And Company Sucrose transporters from plants
WO2000043529A1 (en) * 1999-01-19 2000-07-27 Unilever Plc Method of increasing the water soluble antioxidant content in mechanically harvested peas

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
CASEY ROD ET AL: "The effect of modifying carbohydrate metabolism on seed protein gene expression in peas.", JOURNAL OF PLANT PHYSIOLOGY, vol. 152, no. 6, June 1998 (1998-06-01), pages 636 - 640, XP001024456, ISSN: 0176-1617 *
CROP SCIENCE, vol. 24, no. 3, 1984, pages 587 - 592, ISSN: 0011-183X *
CROP SCIENCE, vol. 31, no. 6, 1991, pages 1600 - 1604, ISSN: 0011-183X *
CROP SCIENCE, vol. 34, no. 1, 1994, pages 131 - 134, ISSN: 0011-183X *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1984, EGLI D B ET AL: "GENOTYPIC VARIATION FOR DURATION OF SEEDFILL IN SOYBEAN GLYCINE-MAX", XP002178052, Database accession no. PREV198478064443 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1991, HANSON W D: "SEED PROTEIN CONTENT AND DELIVERY OF ASSIMILATES TO SOYBEAN SEED EMBRYOS", XP002178050, Database accession no. PREV199293036315 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1994, HANSON W D ET AL: "Control for rate of seed development and seed yield potential in soybean.", XP002178051, Database accession no. PREV199497166164 *
KOMARI T ET AL: "VECTORS CARRYING TWO SEPARATE T-DNAS FOR CO-TRANSFORMATION OF HIGHER PLANTS MEDIATED BY AGROBACTERIUM TUMEFACIENS AND SEGREGATION OF TRANSFORMANTS FREE FROM SELECTION MARKERS", PLANT JOURNAL, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 10, no. 1, 1996, pages 165 - 174, XP002912847, ISSN: 0960-7412 *
PEREZ M D ET AL: "Seed protein content and composition of near-isogenic and induced mutant pea lines.", SEED SCIENCE RESEARCH, vol. 3, no. 3, 1993, pages 187 - 194, XP001024455, ISSN: 0960-2585 *
WEBER HANS ET AL: "Antisense-inhibition of ADP-glucose pyrophosphorylase in developing seeds of Vicia narbonensis moderately decreases starch but increases protein content and affects seed maturation.", PLANT JOURNAL, vol. 24, no. 1, October 2000 (2000-10-01), pages 33 - 43, XP002178049, ISSN: 0960-7412 *

Also Published As

Publication number Publication date
AU7382001A (en) 2001-10-15
AR027750A1 (es) 2003-04-09
DE10115762A1 (de) 2001-12-06
EP1268831A2 (de) 2003-01-02
WO2001075128A2 (de) 2001-10-11

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