WO2001070240A1 - Utilisation de sels d'alkylammonium comme reactifs de crystallisation de proteine - Google Patents

Utilisation de sels d'alkylammonium comme reactifs de crystallisation de proteine Download PDF

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Publication number
WO2001070240A1
WO2001070240A1 PCT/US2001/008425 US0108425W WO0170240A1 WO 2001070240 A1 WO2001070240 A1 WO 2001070240A1 US 0108425 W US0108425 W US 0108425W WO 0170240 A1 WO0170240 A1 WO 0170240A1
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WIPO (PCT)
Prior art keywords
salt
compound
protein
salt product
alkylammonium
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Application number
PCT/US2001/008425
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English (en)
Inventor
Robert A. Ii Flowers
Gloria E. O. Borgstahl
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The University Of Toledo
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Publication date
Application filed by The University Of Toledo filed Critical The University Of Toledo
Publication of WO2001070240A1 publication Critical patent/WO2001070240A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/28—Compounds containing heavy metals
    • A61K31/295—Iron group metal compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B29/00—Single crystals or homogeneous polycrystalline material with defined structure characterised by the material or by their shape
    • C30B29/54—Organic compounds
    • C30B29/58—Macromolecular compounds
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B7/00—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

Definitions

  • the present invention relates the use of alkylammonium salts in protein crystallography.
  • Ethylammonium nitrate a low-melting organic salt
  • EAN Ethylammonium nitrate
  • EAN has water-like characteristics including a capacity for hydrogen bonding and the promotion of micelle formation by some surfactants (Evans, D.F., Yamauchi, A., Roman, R. & Casassa, E.Z. (1982) J. Colloids Interface Sci. 88, 89-96).
  • the present invention relates to a method for preparing a stable protein solvent and crystallization reagent. At least one alkylammonium salt having hydrophobic and ionic characteristics and having the ability to hydrogen bond is combined with a suitable compound to form a precipitate or crystal of the protein.
  • the alkylammonium salt has the formula
  • Another aspect of the present invention relates to a method for purifying an alkylammonium salt. A suitable amount of an acid is cooled.
  • a suitable amount of an alkyl amine is added to the cooled acid to form an alkylammonium salt product.
  • the salt product is stirred for a suitable length of time over charcoal pellets.
  • the salt product is gravity filtered.
  • the charcoal is rinsed with a suitable alcohol to remove any residual salt product.
  • the salt product is lyophilized for a suitable length of time.
  • the alkylammonium salts are especially useful solvents and crystallization reagents and are especially useful as precipitating agent in protein crystallization.
  • the present invention relates to the use of alkylammonium salts as useful solvents for applications in protein chemistry. According to the present invention, proteins treated with an alkylammonium salt are stable. Alkylammonium salts that are contemplated to be within the scope of the present invention have the structure:
  • EAN which is an organic salt and has ionic character in addition to the hydrophobic character contributed by the ethyl substituent, is used to treat the protein lysozyme. Lysozyme, when treated with neat EAN and heated to 333°K for 6 h, retains 45% activity after such treatment, compared with a 2% retention of activity when treated similarly with DMSO.
  • Ethylamine was purchased from Aldrich.
  • Lysozyme (L-7773) was purchased from Sigma.
  • Nitric acid, sodium acetate and glacial acetic acid were obtained from JT Baker.
  • Lysozyme crystallized from EAN has two crystal forms.
  • EAN was synthesized from nitric acid and ethylamine in the following manner. 150 g of 70% nitric acid was added to a 500 ml round-bottom flask. The acid was stirred on ice to allow it to cool. An excess of 70% ethylamine was added drop wise with stirring to the nitric acid. The EAN was stirred overnight with charcoal pellets and then gravity filtered, rinsing the charcoal with methanol to wash off any residual EAN. The product was then lyophilized for one week. The final product was characterized by 1 H NMR.
  • Hen egg-white lysozyme was dissolved to 50 mg ml "1 in 100 xrxM acetate buffer pH 4.5.
  • Hanging-drop crystallization trays were set up with a 500 ⁇ l reservoir solution that contained 100 m/W acetate buffer pH 4.5-5.6 and 300-500 m EAN.
  • the 10 ⁇ hanging drop contained 5 ⁇ of protein solution and 5 ⁇ of reservoir solution.
  • Monoclinic crystals grew at pH 4.5-4.6 over an EAN concentration range of 300-500 mM.
  • the tetragonal form grows at pH 5.4-5.6 at an EAN concentration of 300-400 mM.
  • Crystals were mounted in a glass capillary and room-temperature X-ray diffraction data were collected to 1.6 A resolution on a Rigaku rotating-anode generator equipped with an R-AXIS IV two image-plate detector system. Diffraction intensities were integrated with DENZO and scaled using SCALEPACK (Otwinowski, Z. and Minor, W. (1997), Methods Enzymol., 276, 307-326).
  • Example 2 Hydrophobic compounds, such as ferrocene which is normally insoluble in water, are found to be soluble in solutions containing as little as 500 mM EAN. EAN has many applications in protein crystallography. Hydrophobic ligands, which are generally difficult to introduce into a protein crystal dissolve in EAN solutions and are more easily delivered into protein crystals grown with EAN. Example 3 Also, some compounds, especially those with exposed hydrophobic regions and that do not respond to traditional precipitating agents, such as peripheral or integral membrane proteins, have improved solubility in the presence of EAN. Additionally, EAN is a useful additive for improving the monodispersity of the compounds with multiple aggregation states and for increasing crystallizability of the compound solution.
  • EAN is a useful additive for improving the monodispersity of the compounds with multiple aggregation states and for increasing crystallizability of the compound solution.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Materials Engineering (AREA)
  • Metallurgy (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un procédé de préparation d'un solvant stable et d'un réactif de cristallisation, consistant à combiner avec la protéine au moins un sel d'alkylammonium possédant des caractéristiques hydrophobes et ioniques ainsi que la faculté de former des liaisons hydrogène avec un composé adéquat, afin de former un précipité de cristaux du composé. Pour purifier le sel d'alkylammonium, on refroidit une quantité adéquate d'un acide, on ajoute une quantité adéquate d'un amine alkyle à l'acide refroidi pour former un sel alkylammonium, on agite le sel pendant un laps de temps adéquat sur des billes de charbon de bois, on filtre le sel par gravité, on rince le charbon de bois à l'aide d'un alcool adéquat pour éliminer le sel résiduel, et on lyophilise le sel pendant un laps de temps adéquat.
PCT/US2001/008425 2000-03-20 2001-03-16 Utilisation de sels d'alkylammonium comme reactifs de crystallisation de proteine WO2001070240A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19085400P 2000-03-20 2000-03-20
US60/190,854 2000-03-20

Publications (1)

Publication Number Publication Date
WO2001070240A1 true WO2001070240A1 (fr) 2001-09-27

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PCT/US2001/008425 WO2001070240A1 (fr) 2000-03-20 2001-03-16 Utilisation de sels d'alkylammonium comme reactifs de crystallisation de proteine

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112469825A (zh) * 2018-09-11 2021-03-09 诺维信公司 用于饲料组合物的稳定颗粒

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4197315A (en) * 1977-08-30 1980-04-08 Mcneil Laboratories, Inc. (2-Amino-2-oxoethoxy)acetic acid compounds, compositions and methods
US4377654A (en) * 1981-11-16 1983-03-22 Polaroid Corporation Polymerization processes and polymer compositions
US5296588A (en) * 1991-12-31 1994-03-22 Lever Brothers Company, Division Of Conopco, Inc. Process of preparing N-substituted aldonamides
US5416170A (en) * 1992-05-27 1995-05-16 Agency Of Defense Development Conductive polymeric composite with high electrical activation density and method for preparing the same
US5492723A (en) * 1990-05-22 1996-02-20 Sepragen Corporation Adsorbent medium
US6024855A (en) * 1997-08-15 2000-02-15 Sachem, Inc. Electrosynthesis of hydroxylammonium salts and hydroxylamine using a mediator

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4197315A (en) * 1977-08-30 1980-04-08 Mcneil Laboratories, Inc. (2-Amino-2-oxoethoxy)acetic acid compounds, compositions and methods
US4377654A (en) * 1981-11-16 1983-03-22 Polaroid Corporation Polymerization processes and polymer compositions
US5492723A (en) * 1990-05-22 1996-02-20 Sepragen Corporation Adsorbent medium
US5296588A (en) * 1991-12-31 1994-03-22 Lever Brothers Company, Division Of Conopco, Inc. Process of preparing N-substituted aldonamides
US5416170A (en) * 1992-05-27 1995-05-16 Agency Of Defense Development Conductive polymeric composite with high electrical activation density and method for preparing the same
US6024855A (en) * 1997-08-15 2000-02-15 Sachem, Inc. Electrosynthesis of hydroxylammonium salts and hydroxylamine using a mediator

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE CAPLUS [online] CAS, (COLUMBUS, OHIO, USA); BENHLIMA ET AL.: "Electrochemistry of fused ethylammonium nitrate at 298 K: determination of a redox potential scale", XP002943699, Database accession no. 1998:129903 *
J. CHIM. PHYS., vol. 95, no. 1, 1998, pages 25 - 44 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112469825A (zh) * 2018-09-11 2021-03-09 诺维信公司 用于饲料组合物的稳定颗粒

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