WO2001066779A3 - L'arabitol ou le ribitol, marqueurs par selection positive - Google Patents

L'arabitol ou le ribitol, marqueurs par selection positive Download PDF

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Publication number
WO2001066779A3
WO2001066779A3 PCT/US2001/007474 US0107474W WO0166779A3 WO 2001066779 A3 WO2001066779 A3 WO 2001066779A3 US 0107474 W US0107474 W US 0107474W WO 0166779 A3 WO0166779 A3 WO 0166779A3
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WO
WIPO (PCT)
Prior art keywords
arabitol
cells
ribitol
disclosed
selection methods
Prior art date
Application number
PCT/US2001/007474
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English (en)
Other versions
WO2001066779A2 (fr
Inventor
Wayne Parrott
Peter Lafayette
Patrick Kane
Original Assignee
Univ Of Georgia Res Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Of Georgia Res Foundation filed Critical Univ Of Georgia Res Foundation
Priority to AU2001240117A priority Critical patent/AU2001240117A1/en
Publication of WO2001066779A2 publication Critical patent/WO2001066779A2/fr
Publication of WO2001066779A3 publication Critical patent/WO2001066779A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers

Abstract

L'invention porte sur de nouveaux procédés et matériaux de sélection de cellules transgéniques et en particulier sur des méthodes de sélection positive consistant à conférer à certaines cellules la capacité de métaboliser certains composés dont de préférence l'arabitol, le ribitol, la raffinose, le saccharose, le mannitol ou leurs combinaisons. On peut de ce fait sélectionner des cellules transformées en les soumettant simplement à un milieu contenant de tels composés. Ladite invention, qui atténue les désavantages et problèmes des méthodes de sélection négative, tels que la destruction inutile des cellules transformées, et la dispersion dans l'environnement de gènes potentiellement nuisibles (par exemple gènes résistants aux antibiotiques ou aux herbicide), porte en outre sur de nouvelles séquences nucléotidiques relatives à l'opéron du E. Coli rtl et au gène de l'arabitol déshydrogénase, et sur des séquences d'acides aminés relatives à leurs produits géniques.
PCT/US2001/007474 2000-03-08 2001-03-08 L'arabitol ou le ribitol, marqueurs par selection positive WO2001066779A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001240117A AU2001240117A1 (en) 2000-03-08 2001-03-08 Arabitol or ribitol as positive selectable markers

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18829100P 2000-03-08 2000-03-08
US60/188,291 2000-03-08
US60/255,595 2000-12-14

Publications (2)

Publication Number Publication Date
WO2001066779A2 WO2001066779A2 (fr) 2001-09-13
WO2001066779A3 true WO2001066779A3 (fr) 2003-01-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/007474 WO2001066779A2 (fr) 2000-03-08 2001-03-08 L'arabitol ou le ribitol, marqueurs par selection positive

Country Status (2)

Country Link
AU (1) AU2001240117A1 (fr)
WO (1) WO2001066779A2 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994020627A1 (fr) * 1993-03-02 1994-09-15 Sandoz Ltd. Selection positive a base de mannose ou de xylose
WO1997044448A1 (fr) * 1996-05-17 1997-11-27 Pioneer Hi-Bred International, Inc. Elements promoteurs conferant une expression genique dans les racines de maniere preferentielle.

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994020627A1 (fr) * 1993-03-02 1994-09-15 Sandoz Ltd. Selection positive a base de mannose ou de xylose
WO1997044448A1 (fr) * 1996-05-17 1997-11-27 Pioneer Hi-Bred International, Inc. Elements promoteurs conferant une expression genique dans les racines de maniere preferentielle.

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
BARTKUS J M ET AL: "CONSTRUCTION OF AN IMPROVED D-ARABINOSE PATHWAY IN ESCHERICHIA COLI K-12", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 165, no. 3, March 1986 (1986-03-01), pages 704 - 709, XP001051850, ISSN: 0021-9193 *
BRUNKER P ET AL: "Structure and function of the genes involved in mannitol, arabitol and glucitol utilization from Pseudomonas fluorescens DSM50106", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 206, no. 1, 5 January 1998 (1998-01-05), pages 117 - 126, XP004102880, ISSN: 0378-1119 *
HEUEL H ET AL: "Genes for D-arabinitol and ribitol catabolism from Klebsiella pneumoniae.", MICROBIOLOGY (READING), vol. 144, no. 6, June 1998 (1998-06-01), pages 1631 - 1639, XP002200661, ISSN: 1350-0872 *
HEUEL HANSJOACHIM ET AL: "Substrate recognition domains as revealed by active hybrids between the D-arabinitol and ribitol transporters from Klebsiella pneumoniae.", JOURNAL OF BACTERIOLOGY, vol. 179, no. 19, 1997, pages 6014 - 6019, XP002200662, ISSN: 0021-9193 *
LAFAYETTE P R ET AL: "A non-antibiotic marker for amplification of plant transformation vectors in E. coli.", PLANT CELL REPORTS, vol. 20, no. 4, June 2001 (2001-06-01), pages 338 - 342, XP002200660, ISSN: 0721-7714 *
LINK C D ET AL: "GENOTYPIC EXCLUSION: A NOVEL RELATIONSHIP BETWEEN THE RIBITOL-ARABITOL AND GALACTITOL GENES OF E. COLI", MOLECULAR AND GENERAL GENETICS, SPRINGER VERLAG, BERLIN,, DE, vol. 189, 1983, pages 337 - 339, XP001051839, ISSN: 0026-8925 *
LINK C D ET AL: "INVERTED REPEATS SURROUND THE RIBITOL-ARABITOL GENES OF E.COLI C", NATURE, vol. 298, 1 July 1982 (1982-07-01), pages 94 - 96, XP001042180, ISSN: 0547-051X *
LOVINY T ET AL: "RIBITOL DEHYDROGENASE OF KLEBSIELLA-AEROGENES SEQUENCE OF THE STRUCTURAL GENE", BIOCHEMICAL JOURNAL, vol. 230, no. 3, 1985, pages 579 - 586, XP008003567, ISSN: 0264-6021 *
POSTMA P W ET AL: "PHOSPHOENOLPYRUVATE: CARBOHYDRATE PHOSPHOTRANSFERASE SYSTEMS OF BACTERIA", MICROBIOLOGICAL REVIEWS, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC, US, vol. 57, no. 3, September 1993 (1993-09-01), pages 543 - 594, XP001051826, ISSN: 0146-0749 *
SCANGOS G A ET AL: "RIBITOL AND D-ARABITOL CATABOLISM IN ESCHERICHIA COLI", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 134, no. 2, May 1978 (1978-05-01), pages 492 - 500, XP001051858, ISSN: 0021-9193 *
TRIMBUR D E ET AL: "ISOLATION AND CHARACTERIZATION OF ESCHERICHIA COLI MUTANTS ABLE TO UTILIZE THE NOVEL PENTOSE L-RIBOSE", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 173, no. 8, 1991, pages 2459 - 2464, XP002056778, ISSN: 0021-9193 *
WONG B ET AL: "D-ARABITOL METABOLISM IN CANDIDA ALBICANS: STUDIES OF THE BIOSYNTHETIC PATHWAY AND THE GENE THAT ENCODES NAD-DEPENDENT D-ARABITOL DEHYDROGENASE", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 175, no. 19, 1 October 1993 (1993-10-01), pages 6314 - 6320, XP002059303, ISSN: 0021-9193 *

Also Published As

Publication number Publication date
WO2001066779A2 (fr) 2001-09-13
AU2001240117A1 (en) 2001-09-17

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