WO2001066773A3 - Nucleic acids that code for oxidosqualene cyclases - Google Patents

Nucleic acids that code for oxidosqualene cyclases Download PDF

Info

Publication number
WO2001066773A3
WO2001066773A3 PCT/US2001/007611 US0107611W WO0166773A3 WO 2001066773 A3 WO2001066773 A3 WO 2001066773A3 US 0107611 W US0107611 W US 0107611W WO 0166773 A3 WO0166773 A3 WO 0166773A3
Authority
WO
WIPO (PCT)
Prior art keywords
code
nucleic acids
oxidosqualene cyclases
oxidosqualene
relates
Prior art date
Application number
PCT/US2001/007611
Other languages
French (fr)
Other versions
WO2001066773A2 (en
Inventor
Gregory T Bryan
Brian Mcgonigle
Carl A Maxwell
Susan M Potter
Der-Chyan Hwang
Original Assignee
Du Pont
Gregory T Bryan
Brian Mcgonigle
Carl A Maxwell
Susan M Potter
Der-Chyan Hwang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Gregory T Bryan, Brian Mcgonigle, Carl A Maxwell, Susan M Potter, Der-Chyan Hwang filed Critical Du Pont
Priority to EP01920274A priority Critical patent/EP1352069A2/en
Priority to US10/221,074 priority patent/US7135624B2/en
Priority to CA002401708A priority patent/CA2401708A1/en
Priority to AU2001247348A priority patent/AU2001247348A1/en
Priority to BR0109258-8A priority patent/BR0109258A/en
Publication of WO2001066773A2 publication Critical patent/WO2001066773A2/en
Publication of WO2001066773A3 publication Critical patent/WO2001066773A3/en
Priority to US11/500,769 priority patent/US20060277636A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/14—Vegetable proteins
    • A23J3/16—Vegetable proteins from soybean
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid

Abstract

This invention relates to an isolated nucleic acid fragment encoding an oxidosqualene cyclase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the isolated polynucleotides of the invention, in sense or antisense orientation, operably linked to a suitable regulatory sequence.
PCT/US2001/007611 2000-03-09 2001-03-09 Nucleic acids that code for oxidosqualene cyclases WO2001066773A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP01920274A EP1352069A2 (en) 2000-03-09 2001-03-09 Nucleic acids that code for oxidosqualene cyclases
US10/221,074 US7135624B2 (en) 2001-03-09 2001-03-09 Enzymes involved in triterpene synthesis
CA002401708A CA2401708A1 (en) 2000-03-09 2001-03-09 Enzymes involved in triterpene synthesis
AU2001247348A AU2001247348A1 (en) 2000-03-09 2001-03-09 Nucleic acids that code for oxidosqualene cyclases
BR0109258-8A BR0109258A (en) 2000-03-09 2001-03-09 Enzymes involved in triterpene synthesis
US11/500,769 US20060277636A1 (en) 2000-03-09 2006-08-08 Enzymes involved in triterpene synthesis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18805400P 2000-03-09 2000-03-09
US60/188,054 2000-03-09

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/500,769 Division US20060277636A1 (en) 2000-03-09 2006-08-08 Enzymes involved in triterpene synthesis

Publications (2)

Publication Number Publication Date
WO2001066773A2 WO2001066773A2 (en) 2001-09-13
WO2001066773A3 true WO2001066773A3 (en) 2003-08-07

Family

ID=22691610

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/007611 WO2001066773A2 (en) 2000-03-09 2001-03-09 Nucleic acids that code for oxidosqualene cyclases

Country Status (5)

Country Link
EP (1) EP1352069A2 (en)
AU (1) AU2001247348A1 (en)
BR (1) BR0109258A (en)
CA (1) CA2401708A1 (en)
WO (1) WO2001066773A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040002105A1 (en) * 2002-05-04 2004-01-01 The Samuel Roberts Noble Foundation Methods of identifying genes for the manipulation of triterpene saponins
US20040010818A1 (en) * 2002-05-09 2004-01-15 Mcgonigle Brian Transgenic plants with a suppressed triterpene level
EP1721983B1 (en) * 2004-02-25 2011-01-05 Meiji Seika Kaisha Ltd. Triterpene hydroxylase
WO2007056347A1 (en) * 2005-11-07 2007-05-18 E. I. Du Pont De Nemours And Company Compositions with increased phytosterol levels obtained from plants with decreased triterpene saponin levels
CN109182244B (en) * 2018-08-01 2021-11-02 天津大学 Method for improving biomass of bacillus natto under high-temperature condition
EP3650542A1 (en) * 2018-11-08 2020-05-13 Fraunhofer Gesellschaft zur Förderung der Angewand Method to obtain low triterpene/triterpenoid-containing natural rubber latex

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995030009A2 (en) * 1994-04-29 1995-11-09 Gatsby Charitable Foundation Identification, production and use of saponin glyclosyl hydrolases

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995030009A2 (en) * 1994-04-29 1995-11-09 Gatsby Charitable Foundation Identification, production and use of saponin glyclosyl hydrolases

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
BACH T J ET AL.: "Cloning of cDNAs or genes encoding enzymes of sterol biosynthesis from plants and other eukaryotes: heterologous expression and complementation analysis of mutations for functional characterization.", PROGRESS IN LIPID RESEARCH, vol. 36, no. 2/3, 1997, pages 197 - 226, XP001031154 *
DATABASE EM_PL EMBL; 5 October 1998 (1998-10-05), KUSHIRO T: "Panax ginseng OSCPNY1 mRNA for beta-Amyrin Synthase, complete cds", XP002191889 *
DATABASE SCISEARCH STN; 1994, TSUKAMOTO C ET AL.: "Genetic improvement of saponin components in soybean", XP002193758 *
HAYASHI H ET AL.: "Molecular cloning and characterization of a cDNA for Glycyrrhiza glabra cycloartenol synthase.", BIOLOGICAL & PHARMACEUTICAL BULLETIN, vol. 23, no. 2, February 2000 (2000-02-01), pages 231 - 234, XP002191881, ISSN: 0918-6158 *
KIKUCHI A ET AL.: "Inheritance and characterization of a null allele for group A acetyl saponins found in a mutant soybean ( Glycine max (L.) Merrill.", BREEDING SCIENCE, vol. 49, no. 3, 1999, pages 167 - 171, XP008001169 *
KUSHIRO T ET AL.: "beta-Amyrin synthase: Cloning of oxidosqualene cyclase that catalyzes the formation of the most popular triterpene among higher plants.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 256, no. 1, August 1998 (1998-08-01), pages 238 - 244, XP002191879 *
LUSAS E W ET AL.: "Soy protein products: Processing and use.", JOURNAL OF NUTRITION, vol. 125, no. 3 SUPPL., 1995, First International Symposium on the Role of Soy in Preventing and Treating Chronic Disease;Mesa, Arizona, USA; February 20-23, 1994, pages 573S - 580S, XP001064997, ISSN: 0022-3166 *
SHIBUYA M ET AL.: "Two branches of the lupeol synthase gene in the molecular evolution of plant oxidosqualene cyclases.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 266, no. 1, November 1999 (1999-11-01), pages 302 - 307, XP002191880, ISSN: 0014-2956 *
TSUKAMOTO C ET AL.: "Factors affecting isoflavone content in soybean seeds: changes in isoflavones, saponins, and composition of fatty acids at different temperatures during seed development", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 43, no. 5, 1995, pages 1184 - 1192, XP002193757, ISSN: 0021-8561 *
TSUKAMOTO C ET AL.: "Genetic and chemical polymorphisms of saponins in soybean seed.", PHYTOCHEMISTRY (OXFORD), vol. 34, no. 5, 1993, pages 1351 - 1356, XP001066501, ISSN: 0031-9422 *
TSUKAMOTO C ET AL.: "Genetic improvement of saponin components in soybean", ACS SYMPOSIUM SERIES, vol. 546, 1994, pages 372 - 379 *
YOSHIKI Y ET AL.: "Relationship between chemical structures and biological activities of triterpenoid saponins from soybean.", BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 62, no. 12, December 1998 (1998-12-01), pages 2291 - 2299, XP002191885, ISSN: 0916-8451 *

Also Published As

Publication number Publication date
WO2001066773A2 (en) 2001-09-13
EP1352069A2 (en) 2003-10-15
CA2401708A1 (en) 2001-09-13
AU2001247348A1 (en) 2001-09-17
BR0109258A (en) 2003-06-03

Similar Documents

Publication Publication Date Title
WO2001023575A3 (en) Wuschel (wus) gene homologs
WO2004001012A3 (en) Plant defensins
WO2001004147A3 (en) Plant inositol polyphosphate phosphatase homologs
WO2002029022A3 (en) Chlorophyllases
WO2002044390A3 (en) Floral development genes
WO2000004166A3 (en) Plant cellulose synthases
WO2000014207A3 (en) Plant farnesyltransferases
WO2001066773A3 (en) Nucleic acids that code for oxidosqualene cyclases
WO2000068405A3 (en) Plant defensins
WO2000004162A3 (en) Genes encoding sulfate assimilation proteins
WO2007022195A3 (en) Nitrate transport components
WO2002077158A3 (en) Root specific, stimulant inducible promoter and its use
WO2002038612A3 (en) Canine and feline tumour-associated antigen 5t4
WO1999053068A3 (en) Sucrose transporters from plants
WO2000005353A3 (en) Chorismate synthase from plants
WO2000075340A3 (en) Magnesium chelatase
WO2000028036A3 (en) Disease resistance factors
WO2000060087A3 (en) Cyclin dependent kinase inhibitor genes from plants
WO2000078958A3 (en) Scorpion toxins from buthotus judaicus
WO2001049840A3 (en) Spf1-related transcription factors
WO2000032777A3 (en) Scorpion toxins
WO2000003026A3 (en) Transcription coactivators
WO2000024772A3 (en) Scorpion toxins
WO2003014372A3 (en) Phytochelatin synthase
WO2001046393A3 (en) Aspartate kinase

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2401708

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 10221074

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2001920274

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2001247348

Country of ref document: AU

WWP Wipo information: published in national office

Ref document number: 2001920274

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2001920274

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP