WO2001066717A3 - Gene targeting method - Google Patents

Gene targeting method Download PDF

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Publication number
WO2001066717A3
WO2001066717A3 PCT/US2001/007051 US0107051W WO0166717A3 WO 2001066717 A3 WO2001066717 A3 WO 2001066717A3 US 0107051 W US0107051 W US 0107051W WO 0166717 A3 WO0166717 A3 WO 0166717A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene targeting
gene
targeting
transformable
organisms
Prior art date
Application number
PCT/US2001/007051
Other languages
French (fr)
Other versions
WO2001066717A8 (en
WO2001066717A9 (en
WO2001066717A2 (en
Inventor
Kent G Golic
Yikang S Rong
Gary N Drews
Original Assignee
Univ Utah
Kent G Golic
Yikang S Rong
Gary N Drews
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Utah, Kent G Golic, Yikang S Rong, Gary N Drews filed Critical Univ Utah
Priority to CA002401677A priority Critical patent/CA2401677A1/en
Priority to EP01920209A priority patent/EP1259600A2/en
Priority to AU4728501A priority patent/AU4728501A/en
Publication of WO2001066717A2 publication Critical patent/WO2001066717A2/en
Publication of WO2001066717A3 publication Critical patent/WO2001066717A3/en
Publication of WO2001066717A8 publication Critical patent/WO2001066717A8/en
Publication of WO2001066717A9 publication Critical patent/WO2001066717A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to a method of gene targeting in a transformable host organism, and compositions useful for carrying out the method. The method of gene targeting provides improvement over previous gene targeting methods since it is generally applicable over a wide variety of transformable organisms. It provides time savings in producing organisms with specific gene modifications, and it does not require a pluripotential cell line. The targeting method of the invention exploits the endogenous cellular process of homologous recombination to implement gene targeting at essentially any known gene.
PCT/US2001/007051 2000-03-03 2001-03-01 Gene targeting method WO2001066717A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002401677A CA2401677A1 (en) 2000-03-03 2001-03-01 Gene targeting method
EP01920209A EP1259600A2 (en) 2000-03-03 2001-03-02 Gene targeting method
AU4728501A AU4728501A (en) 2000-03-03 2001-03-02 Gene targeting method

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US18722000P 2000-03-03 2000-03-03
US60/187,220 2000-03-03
US18867200P 2000-03-13 2000-03-13
US60/188,672 2000-03-13
US25868200P 2000-12-28 2000-12-28
US60/258,682 2000-12-28

Publications (4)

Publication Number Publication Date
WO2001066717A2 WO2001066717A2 (en) 2001-09-13
WO2001066717A3 true WO2001066717A3 (en) 2002-03-14
WO2001066717A8 WO2001066717A8 (en) 2002-04-18
WO2001066717A9 WO2001066717A9 (en) 2003-01-16

Family

ID=27392215

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/007051 WO2001066717A2 (en) 2000-03-03 2001-03-01 Gene targeting method

Country Status (4)

Country Link
EP (1) EP1259600A2 (en)
AU (1) AU4728501A (en)
CA (1) CA2401677A1 (en)
WO (1) WO2001066717A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0126251D0 (en) * 2001-11-01 2002-01-02 Imp College Innovations Ltd Methods
WO2003087341A2 (en) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
EP1572919A4 (en) * 2002-04-30 2006-03-29 Tosk Inc Methods and compositions for use in homologous recombination
US7164056B2 (en) 2002-05-03 2007-01-16 Pioneer Hi-Bred International, Inc. Gene targeting using replicating DNA molecules
EP2806025B1 (en) 2002-09-05 2019-04-03 California Institute of Technology Use of zinc finger nucleases to stimulate gene targeting
DE10251918A1 (en) * 2002-11-08 2004-05-19 Horn, Carsten, Dipl.-Biochem. Dr. Inheritable integration of DNA into germ-line invertebrate cells, useful for large-scale production of transgenic insects, includes post-transformational removal of mobilizable elements to increase stability
US7700356B2 (en) 2002-11-08 2010-04-20 The United States Of America As Represented By The Secretary Of Agriculture System for gene targeting and producing stable genomic transgene insertions
EP1445320A1 (en) * 2003-02-05 2004-08-11 ARTEMIS Pharmaceuticals GmbH Automated gene-targeting using non-toxic detectable markers
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
EP2361984A1 (en) 2003-10-09 2011-08-31 E. I. du Pont de Nemours and Company Gene silencing by using modified micro-RNA molecules
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
EP2664673A3 (en) 2006-10-05 2014-02-19 E. I. du Pont de Nemours and Company Maize microRNA sequences
US20150240253A1 (en) 2012-08-30 2015-08-27 E. I. Du Pont De Nemours And Company Long intergenic non-coding rnas in maize
CN113073140B (en) * 2021-04-22 2022-02-22 宁波大学 PCR detection primer group and method for simultaneously identifying 7 meat-derived foods

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997047758A1 (en) * 1996-06-14 1997-12-18 Massachusetts Institute Of Technology Methods and compositions for transforming cells
EP0957165A2 (en) * 1997-12-01 1999-11-17 Roche Diagnostics GmbH Optimizing cells for endogenous gene activation
WO2000009755A2 (en) * 1998-08-12 2000-02-24 Pangene Corporation Domain specific gene evolution
WO2000028008A1 (en) * 1998-11-10 2000-05-18 Maxygen, Inc. Modified ribulose 1,5-bisphosphate carboxylase/oxygenase
WO2000063365A1 (en) * 1999-04-21 2000-10-26 Pangene Corporation Locked nucleic acid hybrids and methods of use
WO2001005961A1 (en) * 1999-07-14 2001-01-25 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997047758A1 (en) * 1996-06-14 1997-12-18 Massachusetts Institute Of Technology Methods and compositions for transforming cells
EP0957165A2 (en) * 1997-12-01 1999-11-17 Roche Diagnostics GmbH Optimizing cells for endogenous gene activation
WO2000009755A2 (en) * 1998-08-12 2000-02-24 Pangene Corporation Domain specific gene evolution
WO2000028008A1 (en) * 1998-11-10 2000-05-18 Maxygen, Inc. Modified ribulose 1,5-bisphosphate carboxylase/oxygenase
WO2000063365A1 (en) * 1999-04-21 2000-10-26 Pangene Corporation Locked nucleic acid hybrids and methods of use
WO2001005961A1 (en) * 1999-07-14 2001-01-25 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same

Also Published As

Publication number Publication date
WO2001066717A8 (en) 2002-04-18
CA2401677A1 (en) 2001-09-13
EP1259600A2 (en) 2002-11-27
WO2001066717A9 (en) 2003-01-16
AU4728501A (en) 2001-09-17
WO2001066717A2 (en) 2001-09-13

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