WO2001062967A3 - A method that compares genomic sequences - Google Patents

A method that compares genomic sequences Download PDF

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Publication number
WO2001062967A3
WO2001062967A3 PCT/IL2001/000151 IL0100151W WO0162967A3 WO 2001062967 A3 WO2001062967 A3 WO 2001062967A3 IL 0100151 W IL0100151 W IL 0100151W WO 0162967 A3 WO0162967 A3 WO 0162967A3
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WO
WIPO (PCT)
Prior art keywords
primers
match
conserved
gene family
dna
Prior art date
Application number
PCT/IL2001/000151
Other languages
French (fr)
Other versions
WO2001062967A2 (en
Inventor
Ben-Zion Vider
Nurit Katzir
Original Assignee
Genena Ltd
Agricultural Res Org
Ben-Zion Vider
Nurit Katzir
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genena Ltd, Agricultural Res Org, Ben-Zion Vider, Nurit Katzir filed Critical Genena Ltd
Priority to AU2001234048A priority Critical patent/AU2001234048A1/en
Publication of WO2001062967A2 publication Critical patent/WO2001062967A2/en
Publication of WO2001062967A3 publication Critical patent/WO2001062967A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Described herein is a PCR method of DNA fingerprinting using primers that match conserved regions of a gene family. In this manner the resulting PCR products represents genomic DNA fragments that are parts of genes. The DNA fingerprints can be those of any source, plant, animal and human. The method uses a plurality of sense primers that match a first conserved region in the gene family, and a plurality of anti-sense primers that match another conserved region in the gene family. By several modifications enabling increased spectrum of variability one set of conserved primers can be replaced by primers that match the sequences of microsatellite repeat, or cis-acting regulatory region or any other random primer. The DNA fingerprinting system can be linked to these genes expression patterns in various organs and in various biological situations.
PCT/IL2001/000151 2000-02-22 2001-02-19 A method that compares genomic sequences WO2001062967A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001234048A AU2001234048A1 (en) 2000-02-22 2001-02-19 A method that compares genomic sequences

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
IL134660 2000-02-22
IL13466000 2000-02-22
IL13712400 2000-07-02
IL137124 2000-07-02
IL13795900 2000-08-20
IL137959 2000-08-20

Publications (2)

Publication Number Publication Date
WO2001062967A2 WO2001062967A2 (en) 2001-08-30
WO2001062967A3 true WO2001062967A3 (en) 2002-08-01

Family

ID=27271923

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2001/000151 WO2001062967A2 (en) 2000-02-22 2001-02-19 A method that compares genomic sequences

Country Status (2)

Country Link
AU (1) AU2001234048A1 (en)
WO (1) WO2001062967A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110172525A (en) * 2019-06-26 2019-08-27 广西壮族自治区林业科学研究院 Forest difference expression gene SSR primer sets and polymorphism SSR marker development approach

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876932A (en) * 1995-05-19 1999-03-02 Max-Planc-Gesellschaft Zur Forderung Der Wissenschaften E V. Berlin Method for gene expression analysis
WO1999034016A2 (en) * 1997-12-29 1999-07-08 Genena Ltd. A method for identifying and characterizing cells and tissues
FR2779154A1 (en) * 1998-05-27 1999-12-03 Bio Merieux PROCESS FOR AMPLIFYING AT LEAST ONE PARTICULAR NUCLEOTIDE SEQUENCE AND PRIMERS FOR IMPLEMENTATION

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876932A (en) * 1995-05-19 1999-03-02 Max-Planc-Gesellschaft Zur Forderung Der Wissenschaften E V. Berlin Method for gene expression analysis
WO1999034016A2 (en) * 1997-12-29 1999-07-08 Genena Ltd. A method for identifying and characterizing cells and tissues
FR2779154A1 (en) * 1998-05-27 1999-12-03 Bio Merieux PROCESS FOR AMPLIFYING AT LEAST ONE PARTICULAR NUCLEOTIDE SEQUENCE AND PRIMERS FOR IMPLEMENTATION

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LOPEZ-NIETO C E AND NIGAM S K: "Selective amplificationof protein-coding regions of large sets of genes using statistically designed primer sets", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 14, 1 July 1996 (1996-07-01), pages 857 - 861, XP002090066, ISSN: 1087-0156 *
STONE B AND WHARTON W: "TARGETED RNA FINGERPRINTING: THE CLONING OF DIFFERENTIALLY-EXPRESSED cDNA FRAGMENTS ENRICHED FOR MEMBERS OF THE ZINC FINGER GENE FAMILY", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 13, no. 22, 1994, pages 2612 - 2618, XP002076945, ISSN: 0305-1048 *
VIDER B ET AL: "Human colorectal carcinogenesis is associated with deregulation of homeobox gene expression", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 232, no. 3, March 1997 (1997-03-01), pages 742 - 748, XP002104685, ISSN: 0006-291X *

Also Published As

Publication number Publication date
AU2001234048A1 (en) 2001-09-03
WO2001062967A2 (en) 2001-08-30

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