WO2001061042A3 - Composite membranes for assaying biomolecules - Google Patents

Composite membranes for assaying biomolecules Download PDF

Info

Publication number
WO2001061042A3
WO2001061042A3 PCT/US2001/004974 US0104974W WO0161042A3 WO 2001061042 A3 WO2001061042 A3 WO 2001061042A3 US 0104974 W US0104974 W US 0104974W WO 0161042 A3 WO0161042 A3 WO 0161042A3
Authority
WO
WIPO (PCT)
Prior art keywords
composite
composite membranes
support
membrane
present
Prior art date
Application number
PCT/US2001/004974
Other languages
French (fr)
Other versions
WO2001061042A2 (en
Inventor
Richard F Salinaro
Isaac Rothman
Thomas C Gsell
Original Assignee
Pall Corp
Richard F Salinaro
Isaac Rothman
Thomas C Gsell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pall Corp, Richard F Salinaro, Isaac Rothman, Thomas C Gsell filed Critical Pall Corp
Priority to CA002399163A priority Critical patent/CA2399163A1/en
Priority to JP2001559878A priority patent/JP2004508533A/en
Priority to AU2001238359A priority patent/AU2001238359A1/en
Priority to EP01910790A priority patent/EP1255997A2/en
Publication of WO2001061042A2 publication Critical patent/WO2001061042A2/en
Publication of WO2001061042A3 publication Critical patent/WO2001061042A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/10—Supported membranes; Membrane supports
    • B01D69/107—Organic support material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/56—Polyamides, e.g. polyester-amides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/02—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/10—Supported membranes; Membrane supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/10—Supported membranes; Membrane supports
    • B01D69/108—Inorganic support material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/0063—Other, e.g. van der Waals forces, hydrogen bonding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00635—Introduction of reactive groups to the surface by reactive plasma treatment
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00641—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being continuous, e.g. porous oxide substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Disclosed are smooth surfaced porous membranes having one or more advantages such as low autofluorescence, thermal-cyclability, especially under humid conditions, and three-dimensional binding capacity. The membrane can be free-standing or, preferably in combination with a support as in a composite membrane. The present invention provides a composite membrane comprising a porous polymer layer disposed on a support. The present invention further provides devices such as microarray devices comprising the composite for the analysis of biomaterials such as nucleic acids.
PCT/US2001/004974 2000-02-18 2001-02-16 Composite membranes for assaying biomolecules WO2001061042A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002399163A CA2399163A1 (en) 2000-02-18 2001-02-16 Composite membranes for assaying biomolecules
JP2001559878A JP2004508533A (en) 2000-02-18 2001-02-16 film
AU2001238359A AU2001238359A1 (en) 2000-02-18 2001-02-16 Membranes
EP01910790A EP1255997A2 (en) 2000-02-18 2001-02-16 Composite membranes for assaying biomolecules

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US18332700P 2000-02-18 2000-02-18
US60/183,327 2000-02-18
US22082500P 2000-07-26 2000-07-26
US60/220,825 2000-07-26

Publications (2)

Publication Number Publication Date
WO2001061042A2 WO2001061042A2 (en) 2001-08-23
WO2001061042A3 true WO2001061042A3 (en) 2002-02-07

Family

ID=26879001

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/004974 WO2001061042A2 (en) 2000-02-18 2001-02-16 Composite membranes for assaying biomolecules

Country Status (5)

Country Link
EP (1) EP1255997A2 (en)
JP (1) JP2004508533A (en)
AU (1) AU2001238359A1 (en)
CA (1) CA2399163A1 (en)
WO (1) WO2001061042A2 (en)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6890483B2 (en) 2000-07-05 2005-05-10 Cuno Incorporated Non-luminescent substrate
EP1297340A2 (en) 2000-07-05 2003-04-02 Cuno Incorporated Low fluorescence nylon/glass composites for microdagnostics
WO2003046508A2 (en) 2001-11-09 2003-06-05 Biomicroarrays, Inc. High surface area substrates for microarrays and methods to make same
EP1490175A2 (en) * 2001-12-10 2004-12-29 Sartorius Ag Microarray device
EP1520037A4 (en) * 2002-02-27 2006-06-07 Miragene Inc Improved substrate chemistry for protein immobilization on a rigid support
JP4678516B2 (en) * 2002-08-16 2011-04-27 アヴァントラ バイオサイエンスィズ コーポレーション Substrates for material separation, reaction, and microscopic analysis
JP2009270963A (en) * 2008-05-08 2009-11-19 Toppan Printing Co Ltd Electrophoretic cassette
CN105611994B (en) * 2013-09-30 2019-06-21 东丽株式会社 Composite semipermeable membrane and its manufacturing method
US10294267B2 (en) 2013-12-04 2019-05-21 Pall Corporation Membrane with surface channels
US9616395B2 (en) 2014-05-30 2017-04-11 Pall Corportaion Membrane comprising self-assembled block copolymer and process for producing the same by spray coating (Ic)
US9593219B2 (en) 2014-05-30 2017-03-14 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by spin coating (IIa)
US9598543B2 (en) 2014-05-30 2017-03-21 Pall Corporation Self-assembled structure and membrane comprising block copolymer and process for producing the same by spin coating (VIa)
US9604181B2 (en) 2014-05-30 2017-03-28 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by spray coating (IIc)
US9592477B2 (en) 2014-05-30 2017-03-14 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by hybrid casting (Ib)
US9593218B2 (en) 2014-05-30 2017-03-14 Pall Corporation Self-assembled structure and membrane comprising block copolymer and process for producing the same by spin coating (IIIa)
US9469733B2 (en) 2014-05-30 2016-10-18 Pall Corporation Self-assembled structure and membrane comprising block copolymer and process for producing the same by spin coating (IVa)
US9169361B1 (en) 2014-05-30 2015-10-27 Pall Corporation Self-assembling polymers—VI
US9193835B1 (en) 2014-05-30 2015-11-24 Pall Corporation Self-assembling polymers—IV
US9593217B2 (en) 2014-05-30 2017-03-14 Pall Corporation Self-assembled structure and membrane comprising block copolymer and process for producing the same by spin coating (Va)
US9765171B2 (en) 2014-05-30 2017-09-19 Pall Corporation Self-assembling polymers—V
US9328206B2 (en) 2014-05-30 2016-05-03 Pall Corporation Self-assembling polymers—III
US9592476B2 (en) 2014-05-30 2017-03-14 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by hybrid casting (IIb)
US9441078B2 (en) 2014-05-30 2016-09-13 Pall Corporation Self-assembling polymers—I
US9162189B1 (en) 2014-05-30 2015-10-20 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by spin coating (Ia)
US9163122B1 (en) 2014-05-30 2015-10-20 Pall Corporation Self-assembling polymers—II
US9962662B2 (en) 2014-06-30 2018-05-08 Pall Corporation Fluorinated polymer and use thereof in the preparation of hydrophilic membranes (vi)
US9254466B2 (en) 2014-06-30 2016-02-09 Pall Corporation Crosslinked cellulosic membranes
US9303133B2 (en) 2014-06-30 2016-04-05 Pall Corporation Hydrophilic membranes and method of preparation thereof (IV)
US9394407B2 (en) 2014-06-30 2016-07-19 Pall Corporation Hydrophilic block copolymers and membranes prepared therefrom (I)
US9309367B2 (en) 2014-06-30 2016-04-12 Pall Corporation Membranes comprising cellulosic material and hydrophilic block copolymer (V)
US9718924B2 (en) 2014-06-30 2017-08-01 Pall Corporation Hydrophilic block copolymers and membranes prepared therefrom (II)
US9260569B2 (en) 2014-06-30 2016-02-16 Pall Corporation Hydrophilic block copolymers and method of preparation thereof (III)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0294105A2 (en) * 1987-06-05 1988-12-07 Pall Corporation Non-fluorescing, non-reflective polyamide for use in diagnostic testing
US5062691A (en) * 1989-10-27 1991-11-05 Minnesota Mining And Manufacturing Company Liquid crystal device with grey scale
EP0605828A1 (en) * 1993-01-04 1994-07-13 Becton, Dickinson and Company Flow-through hybridization assay for oligonucleotide sequences
EP0614987A1 (en) * 1993-02-26 1994-09-14 Becton, Dickinson and Company Flow-through membrane nucleic acid hybridization assay

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0294105A2 (en) * 1987-06-05 1988-12-07 Pall Corporation Non-fluorescing, non-reflective polyamide for use in diagnostic testing
US5062691A (en) * 1989-10-27 1991-11-05 Minnesota Mining And Manufacturing Company Liquid crystal device with grey scale
EP0605828A1 (en) * 1993-01-04 1994-07-13 Becton, Dickinson and Company Flow-through hybridization assay for oligonucleotide sequences
EP0614987A1 (en) * 1993-02-26 1994-09-14 Becton, Dickinson and Company Flow-through membrane nucleic acid hybridization assay

Also Published As

Publication number Publication date
CA2399163A1 (en) 2001-08-23
AU2001238359A1 (en) 2001-08-27
EP1255997A2 (en) 2002-11-13
JP2004508533A (en) 2004-03-18
WO2001061042A2 (en) 2001-08-23

Similar Documents

Publication Publication Date Title
WO2001061042A3 (en) Composite membranes for assaying biomolecules
WO2001091873A3 (en) Polysulfonamide matrices
WO2008153828A3 (en) Method for preparing a porous inorganic coating on a porous support using certain pore formers
WO2007038040A3 (en) Solid polymer electrolyte and process for making same
WO2003046144A3 (en) Microscale immobilization of molecules using a hydrogel and methods of use thereof
AU2003260009A1 (en) Porous composite membrane and method for making the same
EP1265080A3 (en) Porous optical materials
WO2002096979A3 (en) Functionalized porous materials and applications in medical devices
AU2002326633A1 (en) Separation column having a photopolymerized sol-gel component and associated methods
AU7261098A (en) Process for making surface modified porous membrane with perfluorocarbon copolymer
AU6380001A (en) Porous ferro- or ferrimagnetic glass particles for isolating molecules
CA2226485A1 (en) Porous composite sheet and process for the production thereof
GB0901597D0 (en) Mixed matrix membranes with small pore molecular sieves and methods for making and using the membranes
WO2005102503A8 (en) Pervaporation composite membranes
WO2003049677A3 (en) Mesoporous permeation layers for use on active electronic matrix devices
WO2000024796A8 (en) Novel ion-conducting materials suitable for use in electrochemical applications and methods related thereto
ATE273111T1 (en) WOOD IMPREGNATED WITH FURAN POLYMER, METHOD FOR THE PRODUCTION AND USES THEREOF
WO2003087398A3 (en) Electrochemical detector systems
WO2001007891A3 (en) Miniaturized cell array methods and apparatus for cell-based screening
AU2003244872A1 (en) Porous molecularly imprinted polymer membranes
WO2002002585A3 (en) Low fluorescence nylon/glass composites for microdiagnostics
ZA9711668B (en) Solid electrolyte membrane with mechanically-enhancing constituent and porous catalytically enhancing constituents.
CA2322009A1 (en) Novel chromatographic device
ZA200709962B (en) P-tert-butylcalix[6]arenes with triacidic functions at 2, 4 and 6 supported liquid membranes support materials comprising the same and use thereof
AU2002364120A1 (en) Sensor for the use in testing biological chemical or environmental samples

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2399163

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2001910790

Country of ref document: EP

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 559878

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 10204224

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2001910790

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 2001910790

Country of ref document: EP