WO2001061036A3 - A method of mapping restriction endonuclease cleavage sites - Google Patents

A method of mapping restriction endonuclease cleavage sites Download PDF

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Publication number
WO2001061036A3
WO2001061036A3 PCT/GB2001/000718 GB0100718W WO0161036A3 WO 2001061036 A3 WO2001061036 A3 WO 2001061036A3 GB 0100718 W GB0100718 W GB 0100718W WO 0161036 A3 WO0161036 A3 WO 0161036A3
Authority
WO
WIPO (PCT)
Prior art keywords
cleavage sites
restriction endonuclease
endonuclease cleavage
mapping restriction
overhang
Prior art date
Application number
PCT/GB2001/000718
Other languages
French (fr)
Other versions
WO2001061036A8 (en
WO2001061036A2 (en
Inventor
Preben Lexow
Original Assignee
Complete Genomics As
Preben Lexow
Towler Philip Dean
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NO20000792A external-priority patent/NO20000792D0/en
Application filed by Complete Genomics As, Preben Lexow, Towler Philip Dean filed Critical Complete Genomics As
Priority to AU2001232140A priority Critical patent/AU2001232140A1/en
Priority claimed from NO20012863A external-priority patent/NO20012863D0/en
Priority claimed from NO20012864A external-priority patent/NO20012864D0/en
Publication of WO2001061036A2 publication Critical patent/WO2001061036A2/en
Publication of WO2001061036A3 publication Critical patent/WO2001061036A3/en
Publication of WO2001061036A8 publication Critical patent/WO2001061036A8/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention provides a two-step sorting procedure where it is possible to scan the overhanging single-stranded ends of nucleic acid fragments quickly and efficiently using solid supports, such as microarrays. Use is made of two different sets of degenerate overhang-adaptors in this regard. The invention also provides new methods and strategies inter alia for collecting information about sequences and cleavage sites that are between the cleavage sites that have generated an overhang pair. An effective method of producing the restriction map, making it easier to create multiple maps, is also described.
PCT/GB2001/000718 2000-02-17 2001-02-19 A method of mapping restriction endonuclease cleavage sites WO2001061036A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001232140A AU2001232140A1 (en) 2000-02-17 2001-02-19 A method of mapping restriction endonuclease cleavage sites

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
NO20000792 2000-02-17
NO20000792A NO20000792D0 (en) 2000-02-17 2000-02-17 Method of mapping restriction sites
NO20012864 2000-02-21
NO20012863 2000-02-27
NO20012863A NO20012863D0 (en) 2001-06-08 2001-06-08 Method of detecting overhangs
NO20012864A NO20012864D0 (en) 2001-06-08 2001-06-08 Method of mapping restriction sites

Publications (3)

Publication Number Publication Date
WO2001061036A2 WO2001061036A2 (en) 2001-08-23
WO2001061036A3 true WO2001061036A3 (en) 2002-09-12
WO2001061036A8 WO2001061036A8 (en) 2004-04-15

Family

ID=27353349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/000718 WO2001061036A2 (en) 2000-02-17 2001-02-19 A method of mapping restriction endonuclease cleavage sites

Country Status (2)

Country Link
AU (1) AU2001232140A1 (en)
WO (1) WO2001061036A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
EP1314783B1 (en) 2001-11-22 2008-11-19 Sloning BioTechnology GmbH Nucleic acid linkers and their use in gene synthesis
GB0308851D0 (en) * 2003-04-16 2003-05-21 Lingvitae As Method
GB0308852D0 (en) * 2003-04-16 2003-05-21 Lingvitae As Method
DK1557464T3 (en) 2004-01-23 2011-01-24 Sloning Biotechnology Gmbh Enzymatic preparation of nucleic acid molecules
WO2005118877A2 (en) 2004-06-02 2005-12-15 Vicus Bioscience, Llc Producing, cataloging and classifying sequence tags
CN100413978C (en) * 2004-12-16 2008-08-27 上海交通大学 Method for detecting sequence of double chain DNA based on procedure of DNA automaton
GB0524069D0 (en) * 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
US9115352B2 (en) 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
CN110012671B (en) * 2016-09-06 2024-05-28 合成Dna技术公司 Normalization of NGS library concentrations
WO2024112803A2 (en) * 2022-11-22 2024-05-30 Yale University Methods and kits for microscopic imaging

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998010095A1 (en) * 1996-09-05 1998-03-12 Brax Genomics Limited Characterising dna
US5728524A (en) * 1992-07-13 1998-03-17 Medical Research Counsil Process for categorizing nucleotide sequence populations
WO1999000519A1 (en) * 1997-06-27 1999-01-07 Lynx Therapeutics, Inc. Method of mapping restriction sites in polynucleotides
US5858656A (en) * 1990-04-06 1999-01-12 Queen's University Of Kingston Indexing linkers
WO1999028505A1 (en) * 1997-12-03 1999-06-10 Curagen Corporation Methods and devices for measuring differential gene expression

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5858656A (en) * 1990-04-06 1999-01-12 Queen's University Of Kingston Indexing linkers
US5728524A (en) * 1992-07-13 1998-03-17 Medical Research Counsil Process for categorizing nucleotide sequence populations
WO1998010095A1 (en) * 1996-09-05 1998-03-12 Brax Genomics Limited Characterising dna
WO1999000519A1 (en) * 1997-06-27 1999-01-07 Lynx Therapeutics, Inc. Method of mapping restriction sites in polynucleotides
WO1999028505A1 (en) * 1997-12-03 1999-06-10 Curagen Corporation Methods and devices for measuring differential gene expression

Also Published As

Publication number Publication date
AU2001232140A1 (en) 2001-08-27
WO2001061036A8 (en) 2004-04-15
WO2001061036A2 (en) 2001-08-23

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