WO2001060997A3 - Male tissue-specific regulatory region and method of using same - Google Patents

Male tissue-specific regulatory region and method of using same Download PDF

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Publication number
WO2001060997A3
WO2001060997A3 PCT/US2001/004527 US0104527W WO0160997A3 WO 2001060997 A3 WO2001060997 A3 WO 2001060997A3 US 0104527 W US0104527 W US 0104527W WO 0160997 A3 WO0160997 A3 WO 0160997A3
Authority
WO
WIPO (PCT)
Prior art keywords
regulatory region
male tissue
tissue
male
relates
Prior art date
Application number
PCT/US2001/004527
Other languages
French (fr)
Other versions
WO2001060997A2 (en
Inventor
Marc C Albertsen
Timothy W Fox
Carl W Garnaat
Gary Huffman
Timmy L Kendall
Original Assignee
Pioneer Hi Bred Int
Marc C Albertsen
Timothy W Fox
Carl W Garnaat
Gary Huffman
Timmy L Kendall
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int, Marc C Albertsen, Timothy W Fox, Carl W Garnaat, Gary Huffman, Timmy L Kendall filed Critical Pioneer Hi Bred Int
Priority to AU2001238177A priority Critical patent/AU2001238177B8/en
Priority to HU0301053A priority patent/HUP0301053A3/en
Priority to EP01910583A priority patent/EP1255825A2/en
Priority to AU3817701A priority patent/AU3817701A/en
Priority to NZ520704A priority patent/NZ520704A/en
Priority to CA2392722A priority patent/CA2392722C/en
Publication of WO2001060997A2 publication Critical patent/WO2001060997A2/en
Publication of WO2001060997A3 publication Critical patent/WO2001060997A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to an isolated nucleic acid sequence encoding the Ms45 male tissue-preferred regulatory region. In one aspect this invention relates to the use of this male tissue-preferred regulatory region in mediating fertility. An example of such use is the production of hybrid seed such as in a male sterility system. The Ms45 male tissue-preferred regulatory region can be operably linked with exogenous genes, such as those encoding cytotoxins, complementary nucleotidic units and inhibitory molecules. This invention also relates to plant cells, plant tissue and differentiated plants which contain the regulatory region in this invention.
PCT/US2001/004527 1998-06-19 2001-02-13 Male tissue-specific regulatory region and method of using same WO2001060997A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2001238177A AU2001238177B8 (en) 1998-06-19 2001-02-13 Male-tissue Specific Regulatory Region and Method of Using Same
HU0301053A HUP0301053A3 (en) 2000-02-15 2001-02-13 Male tissue-specific regulatory region and method of using same
EP01910583A EP1255825A2 (en) 2000-02-15 2001-02-13 Male tissue-specific regulatory region and method of using same
AU3817701A AU3817701A (en) 2000-02-15 2001-02-13 Male tissue-preferred regulatory region and method of using same
NZ520704A NZ520704A (en) 2000-02-15 2001-02-13 Male tissue-preferred regulatory region and method of using same
CA2392722A CA2392722C (en) 2000-02-15 2001-02-13 Male tissue-preferred regulatory region and method of using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50448700A 2000-02-15 2000-02-15
US09/504,487 2000-02-15

Publications (2)

Publication Number Publication Date
WO2001060997A2 WO2001060997A2 (en) 2001-08-23
WO2001060997A3 true WO2001060997A3 (en) 2002-03-07

Family

ID=24006485

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/004527 WO2001060997A2 (en) 1998-06-19 2001-02-13 Male tissue-specific regulatory region and method of using same

Country Status (6)

Country Link
EP (1) EP1255825A2 (en)
AU (1) AU3817701A (en)
CA (1) CA2392722C (en)
HU (1) HUP0301053A3 (en)
NZ (1) NZ520704A (en)
WO (1) WO2001060997A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070169227A1 (en) * 2003-12-16 2007-07-19 Pioneer Hi-Bred International Inc. Dominant Gene Suppression Transgenes and Methods of Using Same
EA201491670A1 (en) * 2012-03-13 2015-07-30 Пайонир Хай-Бред Интернэшнл, Инк. GENETIC REDUCTION OF MALE REPRODUCTIVE FUNCTION IN PLANTS
EP2825656A1 (en) 2012-03-13 2015-01-21 Pioneer Hi-Bred International Inc. Genetic reduction of male fertility in plants
MX2014011043A (en) 2012-03-13 2015-06-02 Pioneer Hi Bred Int Genetic reduction of male fertility in plants.
US9803214B2 (en) 2013-03-12 2017-10-31 Pioneer Hi-Bred International, Inc. Breeding pair of wheat plants comprising an MS45 promoter inverted repeat that confers male sterility and a construct that restores fertility

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996017945A1 (en) * 1994-12-08 1996-06-13 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
WO1996040925A2 (en) * 1995-06-07 1996-12-19 Pioneer Hi-Bred International, Inc. Regulatory elements conferring tapetum specificity
WO1998059061A1 (en) * 1997-06-23 1998-12-30 Pioneer Hi-Bred International, Inc. Male tissue-preferred regulatory region and method of using same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996017945A1 (en) * 1994-12-08 1996-06-13 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
WO1996040925A2 (en) * 1995-06-07 1996-12-19 Pioneer Hi-Bred International, Inc. Regulatory elements conferring tapetum specificity
WO1998059061A1 (en) * 1997-06-23 1998-12-30 Pioneer Hi-Bred International, Inc. Male tissue-preferred regulatory region and method of using same

Also Published As

Publication number Publication date
WO2001060997A2 (en) 2001-08-23
CA2392722C (en) 2010-05-11
AU3817701A (en) 2001-08-27
HUP0301053A3 (en) 2004-10-28
CA2392722A1 (en) 2001-08-23
NZ520704A (en) 2005-05-27
EP1255825A2 (en) 2002-11-13
HUP0301053A2 (en) 2003-08-28

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