WO2001049856A3 - Drosophila enzymes, encoding nucleic acids and methods of use - Google Patents

Drosophila enzymes, encoding nucleic acids and methods of use Download PDF

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Publication number
WO2001049856A3
WO2001049856A3 PCT/US2001/000360 US0100360W WO0149856A3 WO 2001049856 A3 WO2001049856 A3 WO 2001049856A3 US 0100360 W US0100360 W US 0100360W WO 0149856 A3 WO0149856 A3 WO 0149856A3
Authority
WO
WIPO (PCT)
Prior art keywords
subject
protein
nucleic acids
methods
encoding nucleic
Prior art date
Application number
PCT/US2001/000360
Other languages
French (fr)
Other versions
WO2001049856A2 (en
Inventor
Thomas J Stout
Kevin Patrick Keegan
Allen James Ebens Jr
Original Assignee
Genoptera Llc
Thomas J Stout
Kevin Patrick Keegan
Allen James Ebens Jr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genoptera Llc, Thomas J Stout, Kevin Patrick Keegan, Allen James Ebens Jr filed Critical Genoptera Llc
Priority to AU32741/01A priority Critical patent/AU3274101A/en
Publication of WO2001049856A2 publication Critical patent/WO2001049856A2/en
Publication of WO2001049856A3 publication Critical patent/WO2001049856A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0335—Genetically modified worms
    • A01K67/0336—Genetically modified Nematodes, e.g. Caenorhabditis elegans
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0337—Genetically modified Arthropods
    • A01K67/0339—Genetically modified insects, e.g. Drosophila melanogaster, medfly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention provides isolated invertebrate enzyme-encoding nucleic acids, and proteins encoded thereby, including lysine-ketoglutarate reductase/saccharopine dehydrogenase (LKR/SDH) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). The subject nucleic acid and protein can be used to genetically modify metazoan invertebrate organisms, such as insects and worms, or cultured cells, resulting in expression or mis-expression of a subject protein. The genetically modified organisms or cells can be used in screening assays to identify candidate compounds which are potential pesticidal agents or therapeutics that interact with a subject protein. They can also be used in methods for studying activity of a subject protein and identifying other genes that modulate the function of, or interact with, a subject gene.
PCT/US2001/000360 2000-01-06 2001-01-05 Drosophila enzymes, encoding nucleic acids and methods of use WO2001049856A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU32741/01A AU3274101A (en) 2000-01-06 2001-01-05 Nucleic acids and polypeptides of invertebrate enzymes and methods of use

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US17497300P 2000-01-06 2000-01-06
US60/174,973 2000-01-06
US18584400P 2000-02-29 2000-02-29
US60/185,844 2000-02-29
US19118900P 2000-03-22 2000-03-22
US60/191,189 2000-03-22

Publications (2)

Publication Number Publication Date
WO2001049856A2 WO2001049856A2 (en) 2001-07-12
WO2001049856A3 true WO2001049856A3 (en) 2002-07-18

Family

ID=27390480

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/000360 WO2001049856A2 (en) 2000-01-06 2001-01-05 Drosophila enzymes, encoding nucleic acids and methods of use

Country Status (2)

Country Link
AU (1) AU3274101A (en)
WO (1) WO2001049856A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9096843B2 (en) 2009-12-01 2015-08-04 Novo Nordisk A/S Peptidyl α-hydroxyglycine α-amidating lyases

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008046A (en) * 1991-10-04 1999-12-28 Ophidian Pharmaceuticals, Inc. Drug and pesticide screening

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008046A (en) * 1991-10-04 1999-12-28 Ophidian Pharmaceuticals, Inc. Drug and pesticide screening

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 23 December 1996 (1996-12-23), XP002181819 *
DATABASE EMBL 24 March 1999 (1999-03-24), XP002181817 *
DATABASE EMBL 24 March 1999 (1999-03-24), XP002181818 *

Also Published As

Publication number Publication date
WO2001049856A2 (en) 2001-07-12
AU3274101A (en) 2001-07-16

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