WO2001048182A3 - Amino acid inducible expression system - Google Patents

Amino acid inducible expression system Download PDF

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Publication number
WO2001048182A3
WO2001048182A3 PCT/EP2000/012887 EP0012887W WO0148182A3 WO 2001048182 A3 WO2001048182 A3 WO 2001048182A3 EP 0012887 W EP0012887 W EP 0012887W WO 0148182 A3 WO0148182 A3 WO 0148182A3
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
expression system
inducible expression
acid inducible
serine
Prior art date
Application number
PCT/EP2000/012887
Other languages
French (fr)
Other versions
WO2001048182A2 (en
Inventor
Steen Holmberg
Samuelsen Camilla Ottendahl
Danny Francois Eve Huylebroeck
Jacques E F J G Remacle
Original Assignee
Vlaams Interuniv Inst Biotech
Steen Holmberg
Samuelsen Camilla Ottendahl
Danny Francois Eve Huylebroeck
Jacques E F J G Remacle
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vlaams Interuniv Inst Biotech, Steen Holmberg, Samuelsen Camilla Ottendahl, Danny Francois Eve Huylebroeck, Jacques E F J G Remacle filed Critical Vlaams Interuniv Inst Biotech
Priority to AU30136/01A priority Critical patent/AU3013601A/en
Publication of WO2001048182A2 publication Critical patent/WO2001048182A2/en
Publication of WO2001048182A3 publication Critical patent/WO2001048182A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

Abstract

The invention relates to an amino acid inducible expression system for eukaryotic cells, especially mammalian cells. In particular, a serine and/or threonine inducible system is described, by which a more than 100-fold induction can be obtained.
PCT/EP2000/012887 1999-12-28 2000-12-18 Amino acid inducible expression system WO2001048182A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU30136/01A AU3013601A (en) 1999-12-28 2000-12-18 Amino acid inducible expression system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99126058.9 1999-12-28
EP99126058 1999-12-28

Publications (2)

Publication Number Publication Date
WO2001048182A2 WO2001048182A2 (en) 2001-07-05
WO2001048182A3 true WO2001048182A3 (en) 2002-05-10

Family

ID=8239753

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/012887 WO2001048182A2 (en) 1999-12-28 2000-12-18 Amino acid inducible expression system

Country Status (2)

Country Link
AU (1) AU3013601A (en)
WO (1) WO2001048182A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2982277B1 (en) * 2011-11-08 2015-08-21 Agronomique Inst Nat Rech INDUCIBLE EXPRESSION CASSETTE AND USES THEREOF

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5503991A (en) * 1985-04-15 1996-04-02 Gist-Brocades Vectors for use in filamentous fungi

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5503991A (en) * 1985-04-15 1996-04-02 Gist-Brocades Vectors for use in filamentous fungi
US5503991C1 (en) * 1985-04-15 2001-05-15 Gist Brocades Nv Vectors for use in filamentous fungi

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BORNAES C. ET AL.: "A regulatory element in the CHA1 promoter which confers inducibility by serine and threonine on Saccharomyces cerevisiae genes.", MOLECULAR AND CELLULAR BIOLOGY, vol. 13, no. 12, 1993, pages 7604 - 7611, XP001010475, ISSN: 0270-7306 *
GOSSEN M. ET AL.: "CONTROL OF GENE ACTIVITY IN HIGHER EUKARYOTIC CELLS BY PROKARYOTIC REGULATORY ELEMENTS", TRENDS IN BIOCHEMICAL SCIENCES, vol. 18, no. 12, 1 December 1993 (1993-12-01), pages 471 - 475, XP002021071, ISSN: 0968-0004 *
RIVERA V. M. ET AL.: "A HUMANIZED SYSTEM FOR PHARMACOLOGIC CONTROL OF GENE EXPRESSION", NATURE MEDICINE, vol. 2, no. 9, 1 September 1996 (1996-09-01), pages 1028 - 1032, XP002018162, ISSN: 1078-8956 *

Also Published As

Publication number Publication date
AU3013601A (en) 2001-07-09
WO2001048182A2 (en) 2001-07-05

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