WO2001046214A3 - Compound comprising a nucleic acid moiety and an organo-silane moiety - Google Patents

Compound comprising a nucleic acid moiety and an organo-silane moiety Download PDF

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Publication number
WO2001046214A3
WO2001046214A3 PCT/EP2000/013100 EP0013100W WO0146214A3 WO 2001046214 A3 WO2001046214 A3 WO 2001046214A3 EP 0013100 W EP0013100 W EP 0013100W WO 0146214 A3 WO0146214 A3 WO 0146214A3
Authority
WO
WIPO (PCT)
Prior art keywords
moiety
organo
compound
silane
nucleic acid
Prior art date
Application number
PCT/EP2000/013100
Other languages
French (fr)
Other versions
WO2001046214A2 (en
Inventor
Martin Huber
Wolfgang Schmidt
Manfred Mueller
Reinhard Hiller
Original Assignee
Lion Bioscience Ag
Martin Huber
Wolfgang Schmidt
Manfred Mueller
Reinhard Hiller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP99125484A external-priority patent/EP1111068A1/en
Priority claimed from EP99125485A external-priority patent/EP1110967A1/en
Application filed by Lion Bioscience Ag, Martin Huber, Wolfgang Schmidt, Manfred Mueller, Reinhard Hiller filed Critical Lion Bioscience Ag
Priority to EP00991249A priority Critical patent/EP1250344A2/en
Priority to AU31631/01A priority patent/AU3163101A/en
Publication of WO2001046214A2 publication Critical patent/WO2001046214A2/en
Publication of WO2001046214A3 publication Critical patent/WO2001046214A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F7/00—Compounds containing elements of Groups 4 or 14 of the Periodic Table
    • C07F7/02—Silicon compounds
    • C07F7/08—Compounds having one or more C—Si linkages
    • C07F7/18—Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
    • C07F7/1804—Compounds having Si-O-C linkages
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Structural Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention concerns a compound comprising a biomolecule moiety and an organo-silane moiety, as well as a process for the synthesis thereof. The invention also concerns a support comprising the biomolecule moiety with the organo-silane moiety, wherein the biomolecule moiety is attached to the support through the organo-silane moiety. The invention also concerns a process for a nucleic acid synthesis reaction making use of the biomolecule moiety with the organo-silane moiety as well as uses of the novel compound. The invention in addition concerns a kit comprising the compound comprising a biomolecule moiety and an organo-silane moiety.
PCT/EP2000/013100 1999-12-21 2000-12-21 Compound comprising a nucleic acid moiety and an organo-silane moiety WO2001046214A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00991249A EP1250344A2 (en) 1999-12-21 2000-12-21 Compound comprising a nucleic acid moiety and an organo-silane moiety
AU31631/01A AU3163101A (en) 1999-12-21 2000-12-21 Compound comprising a nucleic acid moiety and an organo-silane moiety

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
EP99125484A EP1111068A1 (en) 1999-12-21 1999-12-21 Branched compound for use in nucleic acid detection and analysis reactions
EP99125484.8 1999-12-21
EP99125485A EP1110967A1 (en) 1999-12-21 1999-12-21 Compound comprising a biomolecule moiety and an organo-silane moiety
EP99125485.5 1999-12-21
US21120900P 2000-06-13 2000-06-13
US21121700P 2000-06-13 2000-06-13
US60/211,217 2000-06-13
US60/211,209 2000-06-13

Publications (2)

Publication Number Publication Date
WO2001046214A2 WO2001046214A2 (en) 2001-06-28
WO2001046214A3 true WO2001046214A3 (en) 2001-12-27

Family

ID=56290094

Family Applications (3)

Application Number Title Priority Date Filing Date
PCT/EP2000/013098 WO2001046464A1 (en) 1999-12-21 2000-12-21 Branched compound for use in nucleic acid detection and analysis reactions
PCT/EP2000/013099 WO2001046213A2 (en) 1999-12-21 2000-12-21 Compound comprising a peptide moiety and an organo-silane moiety.
PCT/EP2000/013100 WO2001046214A2 (en) 1999-12-21 2000-12-21 Compound comprising a nucleic acid moiety and an organo-silane moiety

Family Applications Before (2)

Application Number Title Priority Date Filing Date
PCT/EP2000/013098 WO2001046464A1 (en) 1999-12-21 2000-12-21 Branched compound for use in nucleic acid detection and analysis reactions
PCT/EP2000/013099 WO2001046213A2 (en) 1999-12-21 2000-12-21 Compound comprising a peptide moiety and an organo-silane moiety.

Country Status (3)

Country Link
EP (3) EP1250344A2 (en)
AU (3) AU2170901A (en)
WO (3) WO2001046464A1 (en)

Families Citing this family (20)

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AU2005241112B2 (en) * 2001-07-13 2009-07-30 Nanosphere, Inc. Method for preparing substrates having immobilized molecules and substrates
EP1509336B1 (en) 2002-05-20 2011-01-19 Danisco US Inc. Synthesis of inorganic structures using templation and catalysis by self assembled repeat protein polymers
US7361731B2 (en) 2002-05-20 2008-04-22 Genencor International, Inc. Peptide derivatives, and their use for the synthesis of silicon-based composite materials
US7534621B2 (en) 2003-02-07 2009-05-19 Canon Kabuhsiki Kashia Method of producing probe medium and method of immobilizing probe using probe medium
US20050142094A1 (en) 2003-03-12 2005-06-30 Manoj Kumar Use of repeat sequence protein polymers in personal care compositions
US7297678B2 (en) 2003-03-12 2007-11-20 Genencor International, Inc. Use of repeat sequence protein polymers in personal care compositions
JP4629046B2 (en) 2003-05-14 2011-02-09 ダニスコ・ユーエス・インコーポレーテッド Repeat sequence protein polymer active agent conjugates, methods and uses
CA2524710C (en) 2003-05-14 2012-07-31 Dow Corning Corporation Controlled release of active agents utilizing repeat sequence protein polymers
WO2005065321A2 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US7940746B2 (en) 2004-08-24 2011-05-10 Comcast Cable Holdings, Llc Method and system for locating a voice over internet protocol (VoIP) device connected to a network
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
WO2013072408A1 (en) * 2011-11-15 2013-05-23 Swetree Technologies Ab Surface-functionalized cellulosic fibres, method of manufacture thereof and uses thereof
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc Sequential sequencing
CN105849264B (en) 2013-11-13 2019-09-27 纽亘技术公司 For identifying the composition and method that repeat sequencing reading
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CA2957633A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system

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WO1996004404A1 (en) * 1994-08-03 1996-02-15 Mosaic Technologies, Inc. Method and apparatus for performing amplification of nucleic acid on supports
WO1999011820A1 (en) * 1997-09-02 1999-03-11 Isis Pharmaceuticals, Inc. Compositions and methods for the identification and quantitation of deletion sequence oligonucleotides in synthetic oligonucleotide preparations
WO1999051773A1 (en) * 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
WO1999057323A1 (en) * 1998-05-04 1999-11-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
DE19825899A1 (en) * 1998-06-10 1999-12-16 Memorec Medical Molecular Rese Immobilized oligo- or polynucleotide product useful for identifying and quantifying polynucleotides

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WO1999011820A1 (en) * 1997-09-02 1999-03-11 Isis Pharmaceuticals, Inc. Compositions and methods for the identification and quantitation of deletion sequence oligonucleotides in synthetic oligonucleotide preparations
WO1999051773A1 (en) * 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
WO1999057323A1 (en) * 1998-05-04 1999-11-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
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Also Published As

Publication number Publication date
WO2001046213A3 (en) 2002-05-10
WO2001046464A1 (en) 2001-06-28
EP1244812A1 (en) 2002-10-02
AU2170901A (en) 2001-07-03
AU2843201A (en) 2001-07-03
EP1250344A2 (en) 2002-10-23
EP1252172A2 (en) 2002-10-30
AU3163101A (en) 2001-07-03
WO2001046213A2 (en) 2001-06-28
WO2001046214A2 (en) 2001-06-28

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