Preparation for concentration reduction of allcrgenic bacteria and fungi and for concentration reduction of mite allergens and catty allergens
Technical field of the invention
The invention deals with a preparation for concentration reduction of bacteria, sporulating bacteπa and fungi (moulds) bonded namely through home dust on the surface of fibrous textile.
The invention deals also with a preparation for reduction of mile allergens concentration, the allergens of which are bonded in home dust.
The invention deals further with a preparation for call}' allergen reduction bonded in home dust.
The prior art
It is known, that one from the principal source of allergens in households, offices, schools and other residential and public rooms are the dust particles, on which the allergens are bonded. Λt the recent living conditions the source of home dust arc mostly the carpeting, covering textile, curtains and similar, e.g. fibrous materials, from which the home dust removal can be provided problematically only. The dust particles are the holders of more groups of allergens. The arc mostly bacteria, sporulating bacteria and moulds, further mite allergens and further as very unpleasant, namely in families with children, the catty allergens are manifested.
The concentration reduction of mentioned allergens in textile is achieved partly by mechanic method, that means thorough vacuum cleaning with effective vacuum cleaner, and partly by application of chemical and disinfecting matters. Λ disadvantage of mechanical removing is its low effectiveness, the disadvantage of chemical way is the usual application of wet method or aerosol and the residuals C used chemically piυduccd matters in the piυccssed textile, which is undesirable also Irom point ol \ ιeu of the health.
The task of the invention is to achieve a prepaiation. which would have a high effectiveness against the principal alleigen gioups existing in textile with the home dusl In the same time it should be unexceptionable in the case, when l esidu l quantities occui m the piocessed textile aflei pioecssing It is also dcsii able, that the piepai ation has to be cheap and its application not demanding
The aim of the invention
hoi dissension of the specific teiminolog used in dilleicnt countπes in the bianch of alleigology and also iiom ti anslalion i casons we
e in the f ollow ing text the definitions of some fuithei used teims allei gen - a substance invoking in an oiganism, mostly in human oiganism. an inadequate defensive leaction, alleigic leaction - inadequate defensive leaction of the oiganism to exli aneous substance - the alleigen, allergenic - capable to invoke an alleigic leaction, alleigenily - ability to invoke an alleigic leaction, allergicid - capable to destioy oi bond oi by othei way paialyze the alleigens
The task lntioduced in the pievious text, e g the ci cation ol a piepai tion able to reduce the alleigenetic influence of allei gens existing in the home dust, is f ulfilled b\ thiee inventions, which aie the piepaiation foi allci genih leduclion of thi ee pπncipal alleigens subsistent in households I hese inventions fulfill the demand ol unit) , as thci e is going on one and same invention idea, similai pi inciplc and on the use ol inventions as allei gicid pieparation
The lin ention is a piepai ation lot conccnti atio icduclion of bactei ia. spoiulat g bactei ia and moulds bonded namel} thi ough dust on the sui lace of fibi ous and woven matenals, namely caipets, cui tams and covci ing textile, w hci e the essence of which is in it, that 100 mass units contains 80 to 100 mass units ol zeolite coins ol a
1 lie pi epai atiυn could contain up to 2 mass units ul an υlhei ininual I i om th aluminosilicatc giυup ol a com size Iiom 30 to 400 μm
The higher effect of the preparation according to the invention is the ability to bond to its surface allergens of bacterial, sporulating bacterial and mould type. The quantity of allergens in fibrous textile is after application of the preparation and after beating or vacuum cleaning of the textile considerably reduced.
Λn invention is also the preparation for concentration reduction of mite allergens bonded in home dust, the essence of which is in it, that 100 mass units contains al least 80 mass units of zeolite corn mixture up to a size of 1000 μm.
The preparation for 100 mass units can contain from 90 to 98 mass units of zeolite corn mixture with size up to 1 000 μm and up to 1 0 mass units of additional mixture of corn size up to 800 μm. which is formed from 6.50 to 8.00 mass units of silica Si02, from 0.90 to 2.00 mass units of aluminium oxide ΛfO ?. from 0.20 to 0.40 mass units of sodium oxide Na20 and/or from 0.30 to 0.45 mass units of potassium oxide K20, whereas it contains up to 0.04 mass units of chemically bonded water.
The preparation for 100 mass units can also contain 90 to 98 mass units of mixture from zeolite corns of size up to 1000 μm and from up to 10 mass units of pumice-stone corns of size up to 800 μm.
The higher effect of the preparation according to the invention is the ability to bond to its surface mite allergens. The quantity of allergens in fibrous textile is after application of the preparation and alter beating or vacuum cleaning of the textile considerably reduced.
An invention is also the preparation for concentration reduction of call) allergens bonded in home dust, the essence of which is in it. that 100 mass units contains 20 to 100 mass units of zeolite corn mixture up to a size of 1 00 μm.
The preparation for 100 mass units can contain up lo 80 mass units of additional mixture of corns of an other mineral from the aluminosilicate group with corn size up to 800 μm.
The hiepaialion aecoiding lo invention can foi a gi v en e;r,e lin 100 inav- iniif, contain from 90 lo 98 mass units of zeolite corn mixture with size up to 1 00 μm and up
to 10 mass units of additional mixture of corn size up to 800 μm, which is formed from 6.50 to 8.00 mass units of silica Si0 . from 0.90 to 2.00 mass units of aluminium oxide A1203. from 0.20 to 0.40 mass units of sodium oxide Na2 and/or from 0.30 to 0.45 mass units of potassium oxide K2 , whereas it contains up to 0.04 mass units of chemically bonded water.
The preparation for 100 mass units can also contain 90 to 98 mass units of mixture from zeolite corns of size up to 1000 μm and from up lo 1 0 mass units of pumice-stone corns of size up to 800 μm.
The higher effect of the preparation according to the invention is the ability to bond to its surface catty allergens. The quantity of allergens in fibrous textile is alter application of the preparation and after beating or vacuum cleaning of the textile considerably reduced.
Examples of embodiment of invention.
Example 1 :
The surface of a carpet has been sprinkled with the preparation composed from a mixture of zeolite corns of size from 50 μm to 400 μm in an amount of 1 0 g for each square meter for checking of the effect of the basic component to the bacteria, sporulating bacteria and moulds. Circa after 1 5 minutes of actuating the preparation has been sucked off from the carpet.
Λs it has been determined by microscopic analysis of the carpet surface, and the carpet fibers respectively, and this before the application of preparation and after 1 5 minutes of actuation, the allergenetic material originally embedded in the carpet has been bonded with high efficiency on the corns of the preparation. The final stale of the carpet milieu has unambiguously proven the high effectiveness of the preparation.
The effectiveness of the preparation to microorganisms usually present in home dust has been further independently checked from the ecological point of view .
In icgaid lo the considciably dilleicnt miciooiganism concentiation in dilleienl inteπors the checking of the clfcctivcncss has been piovided with laboiatoi) expeiimcnts on samples of home dust ol following types
■
- with noimal legimc of dust (household no 1 m the table),
- with mcicased fiequency of vacuum-cleaning (household no 2),
- in an office
Wheie CPB = the total nu bei of bacteiia CPSB = the total numbei of spoiulalmg bactei ia CPP = the total numbei of moulds healed = application of 1.65 g ol piepaiation to 1 g ol dust nd not detected, e g less then 50 miciooiganisms one giam ol dust
I he miciobiological analysis ol the dust and the dust with the piepaiation it has been pioven. that a consideiable pai t ol mici ooiganisms has lemamed bonded lo the pai tides of the piepaiation and has been not iclcased to the solution
Example 2.
Wilh a piepai aliυn composed Iiom 85 nuv,
ol a inrdm c ol Λ OI II. ol IΛ
Iiom 80 μm lo 900 μm and 1 5 mass units ol silica gel I he pi epaiation has b en
sprinkled on the carper surface in an amount 5 to 1 0 g for each square meter. Circa after 20 minutes of actuating the preparation has been sucked off from the carpet. A similar effectiveness at bonding of the microorganisms to the preparation as in the example no. 1 has been proven.
Example 3 :
The preparations according to previous examples have been completed with eucalyptus oil as aromatic substance so, that the aromatic substance formed 0. 1 mass units from the total. The effects of the preparation remained unchanged.
The examples of embodiment of the invention no. 1 to 3 p n es the allergicid effects of the preparation to bacteria, sporulating bacteria and moulds bonded in home dust.
Example 4:
A preparation in powdered form with particles of 0.2 lo 0.4 mm in size, which has been composed from 92 mass units of zeolite corns with 50 μm to 1000 μm in size and from 8 mass units of pumice stone corns with size up to 800 μm, has been gently dispersed on a part of a carpet and cushioned furniture in an amount of 5 g/m\ where on the surface of which the dead bodies of mites of type Dli nnatophagoide.s pteronyssinus. including their excrements and the disintegration products of their dead bodies had been previously applied. After one hour exposition of the preparation the presence of allergens of mites in dust from treated and not treated surfaces has been tested with an Λcarex lest, applied to 0.3 g of finer dust particles from the tested surfaces. With such Acarc.x test the quantity of mile allergens in the dust is determined using the following evaluation: level "0" = negative finding level " 1 " = slightly positive finding, e.g. slight pollution with miles level "2" = middle positive finding, e.g. middle pollution with mites level "3" = heavy positive finding, e.g. heavy pollution with mites
The above determined levels of the test results correspond to the guanine quantity dissolved in a liquid composed from 3.7% part of potassium hydroxide KOI I solution, from 72.3% part of melhanol CI I3OI I and from 24%) part of water. The Level " 1 " then corresponds to the quantity 0.06 mg quanine/ml of the solution and the level "3" corresponds to the quantity 0.6 mg guanine/ml of solution.
From the above stated is evident, that in the dust collected from the surface of carpets and cushioning treated with the preparation, there is after vacuum cleaning substantially more bonded mite allergens present, which is proving the efficiency of the preparation.
Example 5:
The dust samples have been enriched with dead bodies of mites of type
Dhermalυphagoides pleronyssiniis in a ratio 1 g of dust to 0.1 g of mites. To such samples the preparation according the invention in a quantity 1 .65 g to 1 . 1 g of the sample has been added. Whereas 100 mass units of the preparation in this example contained: 90 mass units of zeolite corns of size from 50 to 1000 μm and
10 mass units of additional mixture with corns of size up to 800 μm, which contained: 7.8 mass units of silica Si02, 1 .5 mass units of aluminium oxide AI2O3, 0.4 mass units of sodium oxide Na20,
0.26 mass units of potassium oxide K2O and 0.04 mass units of chemically bonded water.
The above mentioned samples have been three time tested with laboratory Acarcx tests, and this both before separation of the preparation and after its separation with wet method. After separation the quantity of quaninc has been determined, firstly in the applied preparation according to invention and secondly in the moslh undissolved pail of tested dust and also in the remained dissolved part of the dust. 1 he collected results are given in the following table:
From the table is evident, that the most part of guanine, e.g. the not desired components with allergenetic effect, is once more bonded to the preparation created according to the invention.
Example 6:
In this example the preparation has been composed from 96 mass units of zeolite corns of 50 μm to 1000 μm in size and the additional mixture has been composed from 4 mass units of pumice stone corns of size up to 800 μm.
Both the practical and the laboratory results have been thoroughly consistent with the result, which had been demonstrated in the previous two examples.
Example 7:
The composition of the preparation has been basically the same with the composition according to the previous two examples, with difference, that the individual mixtures have been completed with up to 1 mass unit of aromatic substance, in the given example with eucalyptus oil. The addition of aromatic substance had not negative influence to the effectiveness of the preparation. The eucalyptus oil can be replaced by a lot of other aromatic substances, some from them had been tested with satisfactory results, that means without negative influence to the preparation effectiveness.
The examples 4 to 7 of embodiment of the invention illustrate the allergicid effect of the preparation according to invention to mite allergens existing in the home dust.
Example 8:
A preparation in powdered form with particles of 0.2 to 1.0 mm in size, which has been composed from 30 mass units of zeolite corns with 50 μm to 1000 μm in size and from 70 mass units of pumice stone corns of 50 up to 800 μm in size, has been in a quantity of 5 g gently homogenized in a grinding mortar with 1 gram of fresh epithelium and piles of home cat. After homogenization the piles and epithelium
e been separated from the particles of the preparation with sieving through a sieve with eyes of 1.0 mm in size. The organic impurities which had been not bonded onto the particles of preparation has been removed with a gentle air stream. To the particles of preparation with bonded organic impurities originated from the cat piles and epithelium a 100 ml quantity of buffer from Na
2Cθ . NaHCOi at pH 9.4 has been added. The wet extraction has been provided at 4°C during 24 hours. The contact of amorphous form of albumin component from the alkaline wet extraction with the surface of aluminosilicates has been checked with microscopic observation.
Example 9:
A preparation in powdered form with particles of 0.2 to 0.4 mm in size, which has been composed from 92 mass units of zeolite corns with 50 μm to 1000 μm in size and from 8 mass units of aluminosilicate corns of other kind of 50 up to 800 μm in size, has been in a quantity of 5 g gently homogenized in a grinding mortar with 1 gram of fresh epithelium and piles of home cat. After homogenizalion the piles and epithelium have been separated from the particles of the preparation with sieving through a sieve with eyes of 1.0 mm in size. The organic impurities which had been not bonded onto the particles of preparation has been removed with a gentle air stream. To the particles of preparation with bonded organic impurities originated from the cat piles and epithelium a 100 ml quantity of buffer from Na2C03, NaHC03 at pH 9.4 has been added. The wet extraction has been provided at 4°C during 24 hours.
The contact and bonding of amorphous form of albumin component from the alkaline wet extraction with the surface of aluminosilicates has been once more checked with microscopic observation, where it was found that even the mentioned less zeolite portion in the preparation decreases the catty allergens concentration.
It should be mentioned for complexity, that the origin of the albumin component bonded on the zeolite particles of the preparation had been provided with a method, which was not object of this invention.
Industrial applicability
The preparation according to the invention can be industrially produced for use in households and in public areas.