WO2001038513A3 - Shuffling of agrobacterium and viral genes, plasmids and genomes for improved plant transformation - Google Patents

Shuffling of agrobacterium and viral genes, plasmids and genomes for improved plant transformation Download PDF

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Publication number
WO2001038513A3
WO2001038513A3 PCT/US2000/032298 US0032298W WO0138513A3 WO 2001038513 A3 WO2001038513 A3 WO 2001038513A3 US 0032298 W US0032298 W US 0032298W WO 0138513 A3 WO0138513 A3 WO 0138513A3
Authority
WO
WIPO (PCT)
Prior art keywords
vectors
agrobacterium
plant
evolved
plasmids
Prior art date
Application number
PCT/US2000/032298
Other languages
French (fr)
Other versions
WO2001038513A2 (en
Inventor
Linda A Castle
Michael Lassner
Kevin Mcbride
James English
Original Assignee
Maxygen Inc
Linda A Castle
Michael Lassner
Kevin Mcbride
James English
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc, Linda A Castle, Michael Lassner, Kevin Mcbride, James English filed Critical Maxygen Inc
Priority to AU18004/01A priority Critical patent/AU1800401A/en
Publication of WO2001038513A2 publication Critical patent/WO2001038513A2/en
Publication of WO2001038513A3 publication Critical patent/WO2001038513A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Abstract

Methods for evolving plant vectors with improved characteristics by recursive recombination are provided. Plant vectors that are RNA or DNA polynucleotides, conjugated-DNA polynucleotides, and plasmids are provided, as are vectors that are agrobacterium strains and plant viruses. Agrobacterium vectors that have evolved such desired properties as broad host range, increased transformation efficiency, insert precision, targeted insertion, and targeting of T-DNA sequences to the chloroplast are provided. Agrobacterium strains, which are amenable to transforming a broad range of host species using simple transformation techniques such as vacuum infiltration or direct infection in planta, are provided. Plant virus vectors are provided that have evolved desired properties, including: rapid systemic spread, reduction of symptoms, and increased protein expression. Use of the evolved vectors to produce transgenic plants is provided. Methods and vectors for producing proteins in transgenic plants and for conferring pathogen-derived resistance are provided.
PCT/US2000/032298 1999-11-23 2000-11-22 Shuffling of agrobacterium and viral genes, plasmids and genomes for improved plant transformation WO2001038513A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU18004/01A AU1800401A (en) 1999-11-23 2000-11-22 Shuffling of agrobacterium and viral genes, plasmids and genomes for improved plant transformation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US16745299P 1999-11-23 1999-11-23
US60/167,452 1999-11-23
US20223300P 2000-05-05 2000-05-05
US60/202,233 2000-05-05

Publications (2)

Publication Number Publication Date
WO2001038513A2 WO2001038513A2 (en) 2001-05-31
WO2001038513A3 true WO2001038513A3 (en) 2002-01-03

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/032298 WO2001038513A2 (en) 1999-11-23 2000-11-22 Shuffling of agrobacterium and viral genes, plasmids and genomes for improved plant transformation

Country Status (2)

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AU (1) AU1800401A (en)
WO (1) WO2001038513A2 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0509460B8 (en) 2004-04-30 2022-06-28 Dow Agrosciences Llc herbicide resistance genes
ES2843401T3 (en) 2005-10-28 2021-07-16 Dow Agrosciences Llc New herbicide resistance genes
EP3260542A1 (en) * 2016-06-20 2017-12-27 Algentech Protein production in plant cells
CN108739402A (en) * 2018-06-24 2018-11-06 合肥扬扬农业科技有限公司 A method of improving corn hybrid seed production efficiency
CN110904144B (en) * 2019-09-18 2022-03-22 四川农业大学 Method for inducing and generating dactulis glomerata hairy roots by utilizing agrobacterium rhizogenes
CN113177973A (en) * 2021-05-24 2021-07-27 四川奇力韦创新科技有限公司 Multi-data fusion processing system and method
CN117286178B (en) * 2023-11-23 2024-03-19 三亚中国农业科学院国家南繁研究院 Simplified construction method of double-component viral vector and related application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998013487A1 (en) * 1996-09-27 1998-04-02 Maxygen, Inc. Methods for optimization of gene therapy by recursive sequence shuffling and selection
WO1999023236A1 (en) * 1997-10-31 1999-05-14 Diversa Corporation Method of dna shuffling
WO1999036516A2 (en) * 1998-01-16 1999-07-22 Biosource Technologies, Inc. Method of determining the function of nucleotide sequences and the proteins they encode by transfecting the same into a host
WO2001007641A2 (en) * 1999-07-21 2001-02-01 Large Scale Biology Corporation Method for expressing a library of nucleic acid sequence variants and selecting desired traits

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998013487A1 (en) * 1996-09-27 1998-04-02 Maxygen, Inc. Methods for optimization of gene therapy by recursive sequence shuffling and selection
WO1999023236A1 (en) * 1997-10-31 1999-05-14 Diversa Corporation Method of dna shuffling
WO1999036516A2 (en) * 1998-01-16 1999-07-22 Biosource Technologies, Inc. Method of determining the function of nucleotide sequences and the proteins they encode by transfecting the same into a host
WO2001007641A2 (en) * 1999-07-21 2001-02-01 Large Scale Biology Corporation Method for expressing a library of nucleic acid sequence variants and selecting desired traits

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LEEMANS J ET AL: "SITE SPECIFIC MUTAGENESIS OF AGROBACTERIUM-TUMEFACIENS TI PLASMIDS AND TRANSFER OF GENES TO PLANT CELLS", JOURNAL OF MOLECULAR AND APPLIED GENETICS, vol. 1, no. 2, 1981, pages 149 - 164, XP001004471, ISSN: 0271-6801 *

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WO2001038513A2 (en) 2001-05-31
AU1800401A (en) 2001-06-04

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