WO2001036679A3 - METHODS FOR GENERATING SINGLE STRANDED cDNA FRAGMENTS - Google Patents

METHODS FOR GENERATING SINGLE STRANDED cDNA FRAGMENTS Download PDF

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Publication number
WO2001036679A3
WO2001036679A3 PCT/US2000/031096 US0031096W WO0136679A3 WO 2001036679 A3 WO2001036679 A3 WO 2001036679A3 US 0031096 W US0031096 W US 0031096W WO 0136679 A3 WO0136679 A3 WO 0136679A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
single stranded
stranded cdna
cdna fragments
generating single
Prior art date
Application number
PCT/US2000/031096
Other languages
French (fr)
Other versions
WO2001036679A2 (en
Inventor
John G Hartwell
Glenn D Hoke
Original Assignee
John G Hartwell
Glenn D Hoke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by John G Hartwell, Glenn D Hoke filed Critical John G Hartwell
Priority to AU16017/01A priority Critical patent/AU1601701A/en
Publication of WO2001036679A2 publication Critical patent/WO2001036679A2/en
Publication of WO2001036679A3 publication Critical patent/WO2001036679A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

New and improved methods are provided for generating amplified DNA fragments from limited quantities of longer DNAs. The methods are robust and reliable, and can be used to provide gene fragments for use in methods of analyzing gene expression patterns.
PCT/US2000/031096 1999-11-15 2000-11-14 METHODS FOR GENERATING SINGLE STRANDED cDNA FRAGMENTS WO2001036679A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU16017/01A AU1601701A (en) 1999-11-15 2000-11-14 Methods for generating single stranded cdna fragments

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16493299P 1999-11-15 1999-11-15
US60/164,932 1999-11-15

Publications (2)

Publication Number Publication Date
WO2001036679A2 WO2001036679A2 (en) 2001-05-25
WO2001036679A3 true WO2001036679A3 (en) 2001-11-22

Family

ID=22596706

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/031096 WO2001036679A2 (en) 1999-11-15 2000-11-14 METHODS FOR GENERATING SINGLE STRANDED cDNA FRAGMENTS

Country Status (2)

Country Link
AU (1) AU1601701A (en)
WO (1) WO2001036679A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691594B2 (en) 1996-04-25 2014-04-08 Bioarray Solutions, Ltd. Method of making a microbead array with attached biomolecules
US8691754B2 (en) 2003-09-22 2014-04-08 Bioarray Solutions, Ltd. Microparticles with enhanced covalent binding capacity and their uses
US9147037B2 (en) 2004-08-02 2015-09-29 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
US9251583B2 (en) 2002-11-15 2016-02-02 Bioarray Solutions, Ltd. Analysis, secure access to, and transmission of array images
US9436088B2 (en) 2001-06-21 2016-09-06 Bioarray Solutions, Ltd. Un-supported polymeric film with embedded microbeads

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
KR20040068122A (en) 2001-10-15 2004-07-30 바이오어레이 솔루션스 리미티드 Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection
NZ547492A (en) 2003-10-28 2009-12-24 Bioarray Solutions Ltd Optimization of gene expression analysis using immobilized capture probes of different lengths and densities
ES2533876T3 (en) * 2003-10-29 2015-04-15 Bioarray Solutions Ltd Multiplexed nucleic acid analysis by double stranded DNA fragmentation

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991005065A1 (en) * 1989-10-02 1991-04-18 Stratagene An improved method of dna sequencing
US5418149A (en) * 1990-07-24 1995-05-23 Hoffmann-La Roche Inc. Reduction of non-specific amplification glycosylase using DUTP and DNA uracil
WO1995033070A1 (en) * 1994-05-26 1995-12-07 New York Medical College Detection of halogenated precursors incorporated into dna
WO1996040902A1 (en) * 1995-06-07 1996-12-19 Trevigen, Inc. Nucleic acid repair enzyme methods for point mutation detection and in vitro mutagenesis
WO1996040901A1 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Template and primer based synthesis of enzymatically cleavable oligonucleotides
WO1999039001A2 (en) * 1998-02-02 1999-08-05 Amersham Pharmacia Biotech Ab Nucleic acid analysis method
WO1999047536A2 (en) * 1998-03-19 1999-09-23 Annette Bernauer Method for conducting the synthesis of nucleic acid molecules
WO1999053087A2 (en) * 1998-04-08 1999-10-21 Europäisches Laboratorium für Molekularbiologie (EMBL) 5'-modified nucleotides and the application thereof in molecular biology and medicine
DE19856796A1 (en) * 1998-12-09 2000-06-15 Biochip Technologies Gmbh Cleavage of chemically synthesized oligo- / polynucleotides at a predetermined location
US6090553A (en) * 1997-10-29 2000-07-18 Beckman Coulter, Inc. Use of uracil-DNA glycosylase in genetic analysis
WO2000065088A2 (en) * 1999-04-26 2000-11-02 Amersham Pharmacia Biotech Ab Primers for identifying typing or classifying nucleic acids

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991005065A1 (en) * 1989-10-02 1991-04-18 Stratagene An improved method of dna sequencing
US5418149A (en) * 1990-07-24 1995-05-23 Hoffmann-La Roche Inc. Reduction of non-specific amplification glycosylase using DUTP and DNA uracil
WO1995033070A1 (en) * 1994-05-26 1995-12-07 New York Medical College Detection of halogenated precursors incorporated into dna
WO1996040902A1 (en) * 1995-06-07 1996-12-19 Trevigen, Inc. Nucleic acid repair enzyme methods for point mutation detection and in vitro mutagenesis
WO1996040901A1 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Template and primer based synthesis of enzymatically cleavable oligonucleotides
US6090553A (en) * 1997-10-29 2000-07-18 Beckman Coulter, Inc. Use of uracil-DNA glycosylase in genetic analysis
WO1999039001A2 (en) * 1998-02-02 1999-08-05 Amersham Pharmacia Biotech Ab Nucleic acid analysis method
WO1999047536A2 (en) * 1998-03-19 1999-09-23 Annette Bernauer Method for conducting the synthesis of nucleic acid molecules
WO1999053087A2 (en) * 1998-04-08 1999-10-21 Europäisches Laboratorium für Molekularbiologie (EMBL) 5'-modified nucleotides and the application thereof in molecular biology and medicine
DE19856796A1 (en) * 1998-12-09 2000-06-15 Biochip Technologies Gmbh Cleavage of chemically synthesized oligo- / polynucleotides at a predetermined location
WO2000065088A2 (en) * 1999-04-26 2000-11-02 Amersham Pharmacia Biotech Ab Primers for identifying typing or classifying nucleic acids

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
D'HAM C ET AL: "Excision of 5,6-dihydoxy-5,6-dihydrothymine, 5,6-dihydrothymine, and 5-hydroxycytosin from defined sequence oligonucleotides by Escherichia coli endonuclease III and fpg proteins: Kinetic and mechanistic aspects", BIOCHEMISTRY,AMERICAN CHEMICAL SOCIETY. EASTON, PA,US, vol. 38, 1999, pages 3335 - 3344, XP002164194, ISSN: 0006-2960 *
VAUGHAN PATRICK ET AL: "A novel process for mutation detection using uracil DNA-glycosylase.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 3, 1 February 1998 (1998-02-01), pages 810 - 815, XP002167705, ISSN: 0305-1048 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691594B2 (en) 1996-04-25 2014-04-08 Bioarray Solutions, Ltd. Method of making a microbead array with attached biomolecules
US9400259B2 (en) 1996-04-25 2016-07-26 Bioarray Solutions, Ltd. Method of making a microbead array with attached biomolecules
US9436088B2 (en) 2001-06-21 2016-09-06 Bioarray Solutions, Ltd. Un-supported polymeric film with embedded microbeads
US9251583B2 (en) 2002-11-15 2016-02-02 Bioarray Solutions, Ltd. Analysis, secure access to, and transmission of array images
US8691754B2 (en) 2003-09-22 2014-04-08 Bioarray Solutions, Ltd. Microparticles with enhanced covalent binding capacity and their uses
US9147037B2 (en) 2004-08-02 2015-09-29 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification

Also Published As

Publication number Publication date
WO2001036679A2 (en) 2001-05-25
AU1601701A (en) 2001-05-30

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