WO2001036649A3 - Isolation and characterization of the genomic dna clones of ribosomal protein gene l25 in tobacco - Google Patents

Isolation and characterization of the genomic dna clones of ribosomal protein gene l25 in tobacco Download PDF

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Publication number
WO2001036649A3
WO2001036649A3 PCT/US2000/031716 US0031716W WO0136649A3 WO 2001036649 A3 WO2001036649 A3 WO 2001036649A3 US 0031716 W US0031716 W US 0031716W WO 0136649 A3 WO0136649 A3 WO 0136649A3
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WO
WIPO (PCT)
Prior art keywords
ribosomal protein
tobacco
characterization
isolation
genomic dna
Prior art date
Application number
PCT/US2000/031716
Other languages
French (fr)
Other versions
WO2001036649A2 (en
Inventor
Li-Fang Shi
Johnway Gao
Brian S Hooker
Ziyu Dai
Original Assignee
Battelle Memorial Institute
Shi Li Fang
Johnway Gao
Brian S Hooker
Ziyu Dai
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Battelle Memorial Institute, Shi Li Fang, Johnway Gao, Brian S Hooker, Ziyu Dai filed Critical Battelle Memorial Institute
Priority to AU17769/01A priority Critical patent/AU1776901A/en
Publication of WO2001036649A2 publication Critical patent/WO2001036649A2/en
Publication of WO2001036649A3 publication Critical patent/WO2001036649A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8225—Leaf-specific, e.g. including petioles, stomata
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The promoter of the ribosomal protein gene L25 is operably linked to a structural gene. This chimeric gene is placed in an expression vector. The expression vector containing the chimeric gene is used to transform plants cells and plants. Seeds are obtained from the transformed plants. A product, such as a protein, encoded by the structural gene is isolated from the transformed plant cells and plants.
PCT/US2000/031716 1999-11-19 2000-11-20 Isolation and characterization of the genomic dna clones of ribosomal protein gene l25 in tobacco WO2001036649A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU17769/01A AU1776901A (en) 1999-11-19 2000-11-20 Isolation and characterization of the genomic dna clones of ribosomal protein gene l25 in tobacco

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16725499P 1999-11-19 1999-11-19
US60/167,254 1999-11-19

Publications (2)

Publication Number Publication Date
WO2001036649A2 WO2001036649A2 (en) 2001-05-25
WO2001036649A3 true WO2001036649A3 (en) 2002-01-03

Family

ID=22606594

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/031716 WO2001036649A2 (en) 1999-11-19 2000-11-20 Isolation and characterization of the genomic dna clones of ribosomal protein gene l25 in tobacco

Country Status (2)

Country Link
AU (1) AU1776901A (en)
WO (1) WO2001036649A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998021348A1 (en) * 1996-11-12 1998-05-22 Battelle Memorial Institute Method of producing human growth factors from whole plants or plant cell cultures

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998021348A1 (en) * 1996-11-12 1998-05-22 Battelle Memorial Institute Method of producing human growth factors from whole plants or plant cell cultures

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DAI ZIYU ET AL: "Promoter elements controlling developmental and environmental regulation of a tobacco ribosomal protein gene L34.", PLANT MOLECULAR BIOLOGY, vol. 32, no. 6, 1996, pages 1055 - 1065, XP002168629, ISSN: 0167-4412 *
DATABASE EMBL SEQUENCE LIBRARY 11 June 1993 (1993-06-11), GAO, J. , ET AL.: "Nucleotide and protein sequences of 60S ribosomal protein L17 from tobacco", XP002173792 *
GAO JIANWEI ET AL: "Developmental and environmental regulation of two ribosomal protein genes in tobacco.", PLANT MOLECULAR BIOLOGY, vol. 25, no. 5, 1994, pages 761 - 770, XP002173791, ISSN: 0167-4412 *
JEENINGA RIENK E ET AL: "Rat RL23a ribosomal protein efficiently competes with its Saccharomyces cerevisiae L25 homologue for assembly into 60 S subunits.", JOURNAL OF MOLECULAR BIOLOGY, vol. 263, no. 5, 1996, pages 648 - 656, XP002173790, ISSN: 0022-2836 *

Also Published As

Publication number Publication date
AU1776901A (en) 2001-05-30
WO2001036649A2 (en) 2001-05-25

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