WO2001033279A1 - Cover glass having mounting medium printed thereon and method of producing the same - Google Patents

Cover glass having mounting medium printed thereon and method of producing the same Download PDF

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Publication number
WO2001033279A1
WO2001033279A1 PCT/JP2000/000125 JP0000125W WO0133279A1 WO 2001033279 A1 WO2001033279 A1 WO 2001033279A1 JP 0000125 W JP0000125 W JP 0000125W WO 0133279 A1 WO0133279 A1 WO 0133279A1
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Prior art keywords
cover glass
printed
mounting medium
encapsulant
producing
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PCT/JP2000/000125
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French (fr)
Japanese (ja)
Inventor
Yozo Muto
Original Assignee
Muto Pure Chemicals Co., Ltd
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Application filed by Muto Pure Chemicals Co., Ltd filed Critical Muto Pure Chemicals Co., Ltd
Publication of WO2001033279A1 publication Critical patent/WO2001033279A1/en

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides

Definitions

  • an encapsulant characterized by forming a film of an encapsulant on a cover glass base plate by printing and then cutting the cover glass base plate is printed. And a method for producing the same.
  • the mounting medium is printed on a cover glass base plate, and then the solvent in the mounting medium is evaporated to form a cover glass base plate. It is smooth and transparent and has a thickness of 1 to 500 ym, preferably 5 to 50 m, and its vertical and horizontal lengths are at least smaller than the required dimensions of the cover glass. A film of the encapsulant, each 1 mm shorter, is formed, and then the cover glass base plate is cut at a portion where the encapsulant film is not printed. More specifically, the present invention relates to a canopy printed with an encapsulant, which is characterized in that a cover glass of a predetermined size is cut out, a glass, and a method of manufacturing the same. Background art
  • Specimens used in clinical examinations such as histopathological section specimens and cytological smears, are stained on slide glass and then alcohol, such as ethanol. It is then dehydrated and cleared with a hydrocarbon solvent such as xylene, and then sealed for storage.
  • the encapsulation is performed by dropping the mounting medium onto a slide glass on which the specimen is placed, and then covering it with a cano and a glass.
  • the mounting medium is a viscous solution of a transparent substance dissolved in a solvent.
  • the solvent in the mounting medium should have a narrow gap between the cover glass and the slide glass. It evaporates from before, and finally the specimen will be embedded in a transparent material between the cover glass and the slide glass, and preserved in this state Is performed well.
  • the mounting medium usually contains stabilizers such as antioxidants and ultraviolet absorbers to prevent fading of stained specimens.
  • one to two (or more) drops of the encapsulating agent are dropped onto the cover glass for each sample, and thereafter, the cover glass is applied. They need to be covered, which is complicated.
  • the encapsulant used in the present invention is a viscous liquid in which a transparent substance is dissolved in a solvent.
  • the glass base plate is 400 to 500 mm long, 400 to 500 mm wide, 0.08 to 0.30 mm thick, preferably 0 . 10 — 0.20 mm in size, which is cut and used for the production of cover glass.
  • the amount of the transparent substance contained in the mounting medium is 20 to 80% by weight of the mounting medium, preferably 30 to 70% by weight. .
  • the shaded area in Fig. 1 is the area where the encapsulant is printed, and the cover glass plate is cut along the solid cutting line (the area where the encapsulant is not printed). Let go.
  • the mounting medium is printed in the center, and there is a portion along the edge where the mounting medium is not printed. ing .
  • the encapsulant film formed is smooth and transparent and has a thickness of 1 to 500 ⁇ , preferably 5 to 500 ⁇ .
  • the vertical and horizontal lengths are each at least 1 mm shorter than the required dimensions of the cover glass.
  • the cover glass is formed in the portion.
  • the solvent required for encapsulation is sufficient for the solvent remaining on the slide glass after being taken out of the xylene tank, and additional solvents should be added. There is no need to drip.
  • Marinol (manufactured by Muto Chemical Co., Ltd.) was used as an encapsulant.
  • a plate manufactured by Dow Corning was used as the raw material for the cover glass.
  • the dimensions were 420 mm in length, 420 mm in width, and 0.155 mm in thickness.
  • a canopy of 24 mm in length and 50 mm in width On the cover glass base plate, a canopy of 24 mm in length and 50 mm in width, In order to manufacture the fabric, a total of 120 sheets of the coating (22 mm in length and 48 mm in width) were printed in 8 rows and 15 rows. The vertical and horizontal directions were separated by 2 mm each.

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

A cover glass adapted for easy, efficient enclosure of a clinical test sample. A method of producing a cover glass of predetermined size having a mounting medium printed thereon, comprising the steps of forming a film of a mounting medium on a cover glass blank by printing, and cutting the cover glass blank along the region where the mounting medium is not printed. Efficient, uniform printing makes it possible to produce a cover glass of stabilized performance having a mounting medium printed thereon.

Description

明 細 書 封入剤 を 印刷 し た カ ノ、'一 ガラ ス 及びその製造方法 技術分野  Description Printed encapsulant printed on cano, glass, and manufacturing method
本発明は、封入剤の被膜を 印刷 に よ り カ バー ガラ ス 素板上に形成さ せ 、 然 る 後に該カ バー ガラ ス 素板を切断す る 事を 特徴 と す る 封入剤 を 印刷 し た カ ノ ー ガラ ス 及びその製造方法に関す る 。  According to the present invention, an encapsulant characterized by forming a film of an encapsulant on a cover glass base plate by printing and then cutting the cover glass base plate is printed. And a method for producing the same.
更 に詳 し く は 、 封入剤 を カ バ ー ガラ ス 素板上 に 印刷 し 、 次 いで封入剤 中 の溶媒 を 蒸発 さ せ る 事 に よ り 、 カ バ一 ガラ ス 用 素板上 に 、 平滑且つ透 明で厚さ 1 ~ 5 0 0 y m 、 好 ま し く は 5 〜 5 0 m で 、 縦及び横の長 さ は 、 カ バ 一 ガラ ス の所定の寸法よ り 、 少な く と も 各 々 1 m m 短 い 、 封入 剤の被膜 を 形成さ せ 、 然 る 後 に 、 該カ バ ー ガラ ス 用 素板 を 、 封入剤の被 膜 が印刷 さ れて い な い部分で切断す る 事に よ り 、 所定の寸法の カ バ ー ガ ラ ス を 切 り 出 す こ と を特徴 と す る 封入剤 を 印刷 し た カ ノ、' 一 ガラ ス 及びそ の製造方法に関 す る 。 背景技術  In more detail, the mounting medium is printed on a cover glass base plate, and then the solvent in the mounting medium is evaporated to form a cover glass base plate. It is smooth and transparent and has a thickness of 1 to 500 ym, preferably 5 to 50 m, and its vertical and horizontal lengths are at least smaller than the required dimensions of the cover glass. A film of the encapsulant, each 1 mm shorter, is formed, and then the cover glass base plate is cut at a portion where the encapsulant film is not printed. More specifically, the present invention relates to a canopy printed with an encapsulant, which is characterized in that a cover glass of a predetermined size is cut out, a glass, and a method of manufacturing the same. Background art
病理組織切片標本、 細胞診塗抹標本等、 臨床検査 に於 い て用 い ら れ る 標本は、 ス ラ イ ドガラ ス 上で染色 さ れた後、 エ タ ノ 一 ル等のア ル コ ー ル 、 次 い で キ シ レ ン等の炭化水素溶媒で脱水透徹 さ れ 、 次 い で保存の為 、 封 入 さ れ る 。  Specimens used in clinical examinations, such as histopathological section specimens and cytological smears, are stained on slide glass and then alcohol, such as ethanol. It is then dehydrated and cleared with a hydrocarbon solvent such as xylene, and then sealed for storage.
封入は 、 封入剤 を標本 を のせ た ス ラ イ ド ガラ ス の上に滴下 し 、 次 い で カ ノ、' 一 ガラ ス を かぶせ る 事に よ っ て行われる 。  The encapsulation is performed by dropping the mounting medium onto a slide glass on which the specimen is placed, and then covering it with a cano and a glass.
通常 、 封入剤 は透明な物質を 、 溶媒に溶解 し た粘稠な溶液であ る 。 Usually, the mounting medium is a viscous solution of a transparent substance dissolved in a solvent.
封入剤 中 の溶媒は、 封入後 、 カ バ 一 ガラ ス と ス ラ イ ド ガラ ス の狭 い隙 間 か ら 蒸発 し 、 最終旳 に はカ バ ー ガ ラ ス と ス ラ イ ド ガラ ス の間の 、 透明 な物質 中 に 、 標本 が埋め込 ま れ る 事 にな り 、 こ の状態で保存が良好 に行 われ る 。 After mounting, the solvent in the mounting medium should have a narrow gap between the cover glass and the slide glass. It evaporates from before, and finally the specimen will be embedded in a transparent material between the cover glass and the slide glass, and preserved in this state Is performed well.
封入剤 に は 、 通常染色 し た標本の退色 を 防止す る 為 に酸化防止剤 、 紫 外線吸収剤等の安定剤 が添加 さ れて い る 。  The mounting medium usually contains stabilizers such as antioxidants and ultraviolet absorbers to prevent fading of stained specimens.
こ の よ う に 、 封入 に際 し て は 、 封入剤を 1 検体毎 に カ バ 一 ガ ラ ス 上 に 1 ~ 2 滴 （ 又 は それ以上 ） 滴下 し 、 然 る 後 、 カ バ 一 ガ ラ ス を かぶせ る 必要 があ り 、 繁雑で あ る 。 As described above, at the time of encapsulation, one to two (or more) drops of the encapsulating agent are dropped onto the cover glass for each sample, and thereafter, the cover glass is applied. They need to be covered, which is complicated.
又 、 滴下 し た封入剤 が 、 カ バ ー ガラ ス 上 に均一 に広 げ る 為 に は 、 か な り 、 熟練 し た技術が必要で あ る 。  Also, in order for the dropped encapsulant to spread evenly on the cover glass, a considerably skilled technique is required.
更 に 、 封入剤 中 に含 ま れ る 溶媒は 、 最終的 に は蒸発 し て 、 空気中 に放 出 さ れ る 為 、 労働衛生上 も 問題があ っ た 。 Furthermore, since the solvent contained in the mounting medium eventually evaporates and is released into the air, there has been a problem in occupational health.
本発明 は、 こ れ ら の問題 を解決す る 為 に行われた も ので あ る 。 発明の 開示 The present invention has been made to solve these problems. Disclosure of the invention
本発明 に於 い て用 い ら れ る 封入剤 は、 透明 な物質 を 、 溶媒 に溶解 し た 粘稠な 液体で あ る 。  The encapsulant used in the present invention is a viscous liquid in which a transparent substance is dissolved in a solvent.
カ ノ、' 一 ガラ ス 用 素板は縦 4 0 0 〜 5 0 0 m m 、横 4 0 0 〜 5 0 0 m m 、 厚 さ 0 . 0 8 ~ 0 . 3 0 m m 、 好 ま し く は 0 . 1 0 — 0 . 2 0 m m の寸 法の も の で あ り 、 こ れ を 切断 し て カ バー ガラ ス の製造に供す る も ので あ る 。  Kano, the glass base plate is 400 to 500 mm long, 400 to 500 mm wide, 0.08 to 0.30 mm thick, preferably 0 . 10 — 0.20 mm in size, which is cut and used for the production of cover glass.
高性能の 印刷機 を 用 い る 事 に よ っ て、 効率良 く 大量の封入剤 を 印刷 し た カ バ 一 ガラ ス を製造す る 事が可能であ る 。  By using a high-performance printing machine, it is possible to efficiently produce a cover glass on which a large amount of mounting medium is printed.
該封入剤 中 に含 ま れ る 透明物質の量は 、 該封入剤の 2 0 ~ 8 0 重量パ 一 セ ン ト 、 好 ま し く は 3 0 ~ 7 0 重量パ ー セ ン ト であ る 。  The amount of the transparent substance contained in the mounting medium is 20 to 80% by weight of the mounting medium, preferably 30 to 70% by weight. .
封入剤 中 の溶媒 を蒸発 さ せ る 為 に は、 常温 、 又 は常温以上の温度で 、 送風乾燥等の方法 を 用 い る 事 が 出来 る 。 図面の簡単な説明 In order to evaporate the solvent in the mounting medium, at room temperature or at a temperature above room temperature, Methods such as blast drying can be used. BRIEF DESCRIPTION OF THE FIGURES
第 1 図の斜線部 は封入剤 が 印刷 さ れて い る 部分で 、 実線の切断線 （ 封 入剤 が印刷 さ れて い な い部分 ） に沿 っ て カ バ ー ガラ ス 素板 を 切 り 放す 。  The shaded area in Fig. 1 is the area where the encapsulant is printed, and the cover glass plate is cut along the solid cutting line (the area where the encapsulant is not printed). Let go.
第 2 図の封入剤 を 印刷 し た カ バ 一 ガラ ス に は 、 封入剤 が 中央部 に 印刷 さ れて お り 、 縁 に 沿 っ て封入剤 が印刷 さ れて い な い部分 が残 っ て い る 。 発明 を 実施す る た めの最良の形態  In the cover glass on which the mounting medium is printed as shown in Fig. 2, the mounting medium is printed in the center, and there is a portion along the edge where the mounting medium is not printed. ing . BEST MODE FOR CARRYING OUT THE INVENTION
本発明 に於 い て 、 封入剤 中 の溶媒 を 蒸発 さ せ た後 、 形成 さ れる 封入剤 の被膜は 、 平滑且つ透明 で 、 厚さ 1 ~ 5 0 0 μ ηη 、 好 ま し く は 5 ~ 5 0 m で 、 縦及 び横の長 さ は 、 カ バ 一 ガラ ス の所定の寸法 よ り 、 各 々 少 な く と も 1 m m 短 く な つ て い る 。  In the present invention, after evaporating the solvent in the encapsulant, the encapsulant film formed is smooth and transparent and has a thickness of 1 to 500 μηη, preferably 5 to 500 μηη. At 50 m, the vertical and horizontal lengths are each at least 1 mm shorter than the required dimensions of the cover glass.
従 っ て 、 該封入剤被膜の間 に は 、 少な く と も 2 m m の 間隔の封入剤 が 印刷 さ れて い な い 部分 が存在す る ので 、 該部分 に於 い て カ バ ー ガラ ス 素 板を 切断す る 事 に よ り 、 所定の寸法の カ バ ー ガラ ス を切 り 出 す事 が 出来 る 。  Accordingly, since there is a portion where the encapsulant is not printed at least at a distance of 2 mm between the encapsulant coatings, the cover glass is formed in the portion. By cutting the blank, it is possible to cut out a cover glass of a predetermined size.
な お 、 封入時 に 必要 な溶媒は 、 キ シ レ ン槽 か ら 取 り 上 げ た後の ス ラ イ ド ガラ ス 上 に残存す る 溶媒で 充分で あ り 、 更 に 溶媒等を 追加 し て滴下す る 必要はな い 。  The solvent required for encapsulation is sufficient for the solvent remaining on the slide glass after being taken out of the xylene tank, and additional solvents should be added. There is no need to drip.
以下に本発明 を 実施例 を 用 い て詳細 に説明す る が 、 こ の実施例 に 限定 さ れる も ので はな い 。  Hereinafter, the present invention will be described in detail with reference to Examples, but it should not be construed that the invention is limited thereto.
封入剤 と し て 、 マ リ ノ ー ル （ 武藤化学株式会社製 ） を 用 い た 。  Marinol (manufactured by Muto Chemical Co., Ltd.) was used as an encapsulant.
カ バ ー ガラ ス 用 素板 と して は、 ダ ウ · コ 一 ニ ン グ社製の も の を 用 い た 。 寸法は、 縦 4 2 0 m m 、 横 4 2 0 m m 、 厚さ 0 . 1 5 5 m m であ っ た 。  As the raw material for the cover glass, a plate manufactured by Dow Corning was used. The dimensions were 420 mm in length, 420 mm in width, and 0.155 mm in thickness.
該カ バ 一 ガ ラ ス 用 素板の上 に 、 縦 2 4 m m 、 横 5 0 m m の カ ノ、' ー ガラ ス を 製造す る た め に被膜 （ 縱 2 2 m m 、 横 4 8 m m ) を 、 縦 8 列 、 横 1 5 列 、 計 1 2 0 枚 印刷 し た 。 縦方 向 、 横方 向 に各 々 2 m m ずつ の 間隔 を あ けた 。 On the cover glass base plate, a canopy of 24 mm in length and 50 mm in width, In order to manufacture the fabric, a total of 120 sheets of the coating (22 mm in length and 48 mm in width) were printed in 8 rows and 15 rows. The vertical and horizontal directions were separated by 2 mm each.
送風乾燥機等 を 用 い て 、 被膜を 十分に乾燥 さ せ た後、 被膜が印刷 さ れ て い な い部分で切断 し 、 所定の寸法の封入剤 を 印刷 し た 力 バ 一 ガラ ス 1 2 0 枚 を製造 し た 。  After using a blow dryer to dry the film sufficiently, cut off the area where the film is not printed, and print the encapsulant with the specified dimensions. 0 pieces were manufactured.
こ の よ う に し て製造 し た封入剤 を 印刷 し た カ ノ 一 ガラ ス を 用 い て 、 手 作業及び 自 動封入機に よ っ て 、 封入を行 っ た 。  Using a canopy glass on which the encapsulant produced in this way was printed, encapsulation was performed manually and by an automatic encapsulation machine.
いずれの場合 も 効率旳で 、 且つ 良好な結果を得た 。 産業上の利用可能性 In each case, efficiency was good and good results were obtained. Industrial applicability
封入剤 を 印刷 し た カ バ ー ガラ ス を 製造す る 際に 、 印刷 し た封入剤の被 膜の間 に 、 封入剤の 印刷 さ れて い な い部分 を 存在さ せ 、 こ の部分で カ バ — ガラ ス 素板 を 切断す る 事 に よ り 、 所定の寸法の封入剤 を 印刷 し た カ バ — ガラ ス を製造す る 事が出来る のであ る 。  When manufacturing a cover glass on which the encapsulant is printed, there is an unprinted part of the encapsulant between the coating films of the encapsulant that has been printed. By cutting the cover glass, it is possible to manufacture a cover glass on which a predetermined size of the encapsulant is printed.

Claims

請 求 の 範 囲 封入剤 を カ バ ー ガラ ス 素板 に 印刷 し 、 次 い で封入剤 中の溶媒 を蒸発 さ せ る 事 に よ り 、 カ バ 一 ガラ ス 用素板上に 、 平滑且つ透明で 、 厚 さ 1 〜 5 0 0 m , 好 ま し く は 5 〜 5 0 / m で 、 縦及 び横の長 さ は 、 力 パ ー ガ ラ ス の所定の寸法 よ り 、 少な く と も 各 々 1 m m 短 い 、 封入剤の被膜を 形成 さ せ 、 然 る 後 に 、 該カ バ 一 ガラ ス 用 素板を 、 封入剤の被膜 が印刷 さ れて い な い 部分で切断す る 事 に よ り 、 所定の寸法の カ バ ー ガラ ス を 切 り 出 す事 を特徴 と す る 封入剤 を 印刷 し た カ バ一 ガラ ス及びその製造方法。 Scope of the request The encapsulant is printed on the cover glass base plate, and then the solvent in the encapsulant is evaporated to provide a smooth and smooth surface on the cover glass base plate. It is transparent and has a thickness of 1 ~ 500m, preferably 5 ~ 50 / m, and its vertical and horizontal lengths are at least less than the specified dimensions of the force glass. The encapsulant film is formed 1 mm shorter each time, and then the cover glass base plate is cut at a portion where the encapsulant film is not printed. A cover glass on which an encapsulant is printed, characterized in that a cover glass having a predetermined size is cut out, and a method of manufacturing the cover glass.
PCT/JP2000/000125 1999-10-27 2000-01-13 Cover glass having mounting medium printed thereon and method of producing the same WO2001033279A1 (en)

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JP34374099A JP2001125006A (en) 1999-10-27 1999-10-27 Cover glass obtained by printing sealing agent and manufacture thereof
JP11/343740 1999-10-27

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AU2005316930B2 (en) * 2004-12-17 2009-06-18 Ventana Medical Systems, Inc. Methods and compositions for a microemulsion-based tissue treatment
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Publication number Priority date Publication date Assignee Title
US3498860A (en) * 1966-08-29 1970-03-03 John E P Pickett Process of mounting precoated cover glass for microscope slides
US4302480A (en) * 1978-06-16 1981-11-24 Merck Patent Gesellschaft Mit Beschrankter Haftung Thin cover sheet for use in microscopic staining and a process for its production
JPS61254916A (en) * 1985-05-07 1986-11-12 Mitsubishi Rayon Co Ltd Cover glass for microscope
US4853262A (en) * 1985-08-13 1989-08-01 Fuji Photo Film Co., Ltd. Cover film for microscopy
JPS6318317A (en) * 1986-07-11 1988-01-26 Kogeisha:Kk Manufacture of preparation
JPH04165321A (en) * 1990-04-28 1992-06-11 Michirou Shibazaki Slide glass and cover glass and manufacture thereof
JPH063231A (en) * 1992-06-18 1994-01-11 Toppan Printing Co Ltd Preparation and manufacture thereof
JPH07113731A (en) * 1993-10-15 1995-05-02 Mutou Kagaku Yakuhin Kk Plate for preparing preparation

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