WO2001033193A3 - Automated plant extraction method and arrays for high throughput screening - Google Patents

Automated plant extraction method and arrays for high throughput screening Download PDF

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Publication number
WO2001033193A3
WO2001033193A3 PCT/US2000/030195 US0030195W WO0133193A3 WO 2001033193 A3 WO2001033193 A3 WO 2001033193A3 US 0030195 W US0030195 W US 0030195W WO 0133193 A3 WO0133193 A3 WO 0133193A3
Authority
WO
WIPO (PCT)
Prior art keywords
solvent
extracts
source material
biochemicals
automated
Prior art date
Application number
PCT/US2000/030195
Other languages
French (fr)
Other versions
WO2001033193A2 (en
WO2001033193A9 (en
Inventor
Gary Eldridge
Marilyn Ghanem
Lu Zeng
Original Assignee
Sequoia Sciences Inc
Gary Eldridge
Marilyn Ghanem
Lu Zeng
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sequoia Sciences Inc, Gary Eldridge, Marilyn Ghanem, Lu Zeng filed Critical Sequoia Sciences Inc
Priority to AU14538/01A priority Critical patent/AU1453801A/en
Publication of WO2001033193A2 publication Critical patent/WO2001033193A2/en
Publication of WO2001033193A3 publication Critical patent/WO2001033193A3/en
Publication of WO2001033193A9 publication Critical patent/WO2001033193A9/en
Priority to US11/358,815 priority patent/US7367933B2/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/18—Devices for withdrawing samples in the liquid or fluent state with provision for splitting samples into portions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00695—Synthesis control routines, e.g. using computer programs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/007—Simulation or vitual synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B01J2219/00707—Processes involving means for analysing and characterising the products separated from the reactor apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/0074—Biological products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N2001/021—Correlating sampling sites with geographical information, e.g. GPS
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N2030/009—Extraction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/062—Preparation extracting sample from raw material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/24—Automatic injection systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/466—Flow patterns using more than one column with separation columns in parallel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/80—Fraction collectors
    • G01N30/82—Automatic means therefor

Abstract

An automated and validated method for preparing plant extracts for use in the high throughput screening of biological assays uses a robotic sample processor for the aspiration and dispensing of solvent extracts and a fully automated solid phase extraction apparatus to produce column fractionated plant extracts. The method comprises the steps of: (a) thoroughly extracting a biological source material with at least one solvent to produce at least one solvent extract containing the potentially bio-active biochemicals of the source material; (b) removing from the solvent extracts interferences, comprising non-selectively bio-active compounds that interfere with biological assays; (c) subjecting the solvent extracts to chromatography by an automated system for handling samples; (d) detecting the potentially selectively bio-active biochemicals of the source material in the eluent; and (e) collecting the biochemicals in isolated fractions having standardized concentrations under control by the automated system with associated data.
PCT/US2000/030195 1999-11-02 2000-11-02 Automated plant extraction method and arrays for high throughput screening WO2001033193A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU14538/01A AU1453801A (en) 1999-11-02 2000-11-02 Automated plant extraction method and arrays for high throughput screening
US11/358,815 US7367933B2 (en) 1999-11-02 2006-02-21 Screening of chemical compounds purified from biological sources

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US16307099P 1999-11-02 1999-11-02
US60/163,070 1999-11-02
US18987200P 2000-03-16 2000-03-16
US60/189,872 2000-03-16
US20963600P 2000-06-06 2000-06-06
US60/209,636 2000-06-06

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/115,741 Continuation-In-Part US20030044846A1 (en) 1999-09-03 2002-04-03 Screening of chemical compounds purified from biological sources

Publications (3)

Publication Number Publication Date
WO2001033193A2 WO2001033193A2 (en) 2001-05-10
WO2001033193A3 true WO2001033193A3 (en) 2001-10-18
WO2001033193A9 WO2001033193A9 (en) 2002-05-30

Family

ID=27388833

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/030195 WO2001033193A2 (en) 1999-11-02 2000-11-02 Automated plant extraction method and arrays for high throughput screening

Country Status (2)

Country Link
AU (1) AU1453801A (en)
WO (1) WO2001033193A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030044846A1 (en) 2001-04-03 2003-03-06 Gary Eldridge Screening of chemical compounds purified from biological sources
DE60117637D1 (en) * 2001-06-05 2006-05-04 Advanced Gene Technology Corp Chip with plant extracts on the surface
KR20040010776A (en) * 2001-06-27 2004-01-31 유니젠 파아마슈티컬스,인크. Method for generating, screening and dereplicating natural product libraries for the discovery of therapeutic agents
GB0205184D0 (en) * 2002-03-06 2002-04-17 Molecularnature Ltd Process for scavenging phytochemicals
US6855343B2 (en) * 2002-05-21 2005-02-15 Advanced Gene Technology, Corp Method to ascertain the quality of herbs
US8305582B2 (en) 2009-09-01 2012-11-06 Alltech Associates, Inc. Methods and apparatus for analyzing samples and collecting sample fractions
CN107941979B (en) * 2017-11-13 2022-07-26 舟山市食品药品检验检测研究院 Method for detecting content of cholesterol oxide in aquatic product
CN110907568A (en) * 2019-12-17 2020-03-24 大连工业大学 Method for extracting free fatty acid from solid or semisolid food by using matrix solid phase dispersion

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5886155A (en) * 1997-06-18 1999-03-23 Bioresources International Inc. Purification of miraculin glycoprotein using tandem hydrophobic interaction chromatography
WO1999020291A2 (en) * 1997-10-23 1999-04-29 Pharmaprint, Inc. Pharmaceutical grade ginkgo biloba

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5886155A (en) * 1997-06-18 1999-03-23 Bioresources International Inc. Purification of miraculin glycoprotein using tandem hydrophobic interaction chromatography
WO1999020291A2 (en) * 1997-10-23 1999-04-29 Pharmaprint, Inc. Pharmaceutical grade ginkgo biloba

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CARDELLINA II J H ET AL: "A chemical screening strategy for the dereplication and prioritization of Hiv-inhibitory aqueous natural products extracts", JOURNAL OF NATURAL PRODUCTS, vol. 56, no. 7, July 1993 (1993-07-01), pages 1123 - 1129, XP002901550 *
CLAESON P ET AL: "Fractionation Protocol for the Isolation of Polypeptides from Plant Biomass", J NAT PROD, vol. 61, no. 1, January 1998 (1998-01-01), pages 77 - 81, XP002901549 *
PHILLIPSON J D: "Radioligand-receptor Binding Assays in the Search for Bioactive Principles from Plants", J PHARM PHARMACOL; BRITISH PHARMACEUTICAL CONF, vol. 51, no. 5, May 1999 (1999-05-01), pages 493 - 503, XP002901551 *

Also Published As

Publication number Publication date
WO2001033193A2 (en) 2001-05-10
WO2001033193A9 (en) 2002-05-30
AU1453801A (en) 2001-05-14

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