WO2001031040A3 - Wirts-vektor-systeme zur überproduktion von thermolabilen enzymen psychrophiler organismen - Google Patents

Wirts-vektor-systeme zur überproduktion von thermolabilen enzymen psychrophiler organismen Download PDF

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Publication number
WO2001031040A3
WO2001031040A3 PCT/EP2000/010593 EP0010593W WO0131040A3 WO 2001031040 A3 WO2001031040 A3 WO 2001031040A3 EP 0010593 W EP0010593 W EP 0010593W WO 0131040 A3 WO0131040 A3 WO 0131040A3
Authority
WO
WIPO (PCT)
Prior art keywords
over
host
order
vector systems
enzymes originating
Prior art date
Application number
PCT/EP2000/010593
Other languages
English (en)
French (fr)
Other versions
WO2001031040A2 (de
WO2001031040A9 (de
Inventor
Thomas Schweder
Tanja Kann
Original Assignee
Thomas Schweder
Tanja Kann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thomas Schweder, Tanja Kann filed Critical Thomas Schweder
Priority to EP00984943A priority Critical patent/EP1224307A2/de
Publication of WO2001031040A2 publication Critical patent/WO2001031040A2/de
Publication of WO2001031040A3 publication Critical patent/WO2001031040A3/de
Publication of WO2001031040A9 publication Critical patent/WO2001031040A9/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Die Erfindung betrifft Systeme zur temperaturregulierten Genexpression, insbesondere zur Überexpression von kälteangepassten, thermolabilen Proteinen aus psychrophilen Organismen.
PCT/EP2000/010593 1999-10-27 2000-10-27 Wirts-vektor-systeme zur überproduktion von thermolabilen enzymen psychrophiler organismen WO2001031040A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP00984943A EP1224307A2 (de) 1999-10-27 2000-10-27 Wirts-vektor-systeme zur überproduktion von thermolabilen enzymen psychrophiler organismen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19951765.7 1999-10-27
DE1999151765 DE19951765A1 (de) 1999-10-27 1999-10-27 Wirts-Vektor-Systeme zur Überproduktion von thermolabilen Enzymen psychrophiler Organismen

Publications (3)

Publication Number Publication Date
WO2001031040A2 WO2001031040A2 (de) 2001-05-03
WO2001031040A3 true WO2001031040A3 (de) 2001-11-08
WO2001031040A9 WO2001031040A9 (de) 2002-09-06

Family

ID=7927061

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/010593 WO2001031040A2 (de) 1999-10-27 2000-10-27 Wirts-vektor-systeme zur überproduktion von thermolabilen enzymen psychrophiler organismen

Country Status (3)

Country Link
EP (1) EP1224307A2 (de)
DE (1) DE19951765A1 (de)
WO (1) WO2001031040A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10334811B4 (de) * 2003-07-30 2007-04-05 Ernst-Moritz-Arndt-Universität Kälte-induzierbares Expressionssystem
JP4336184B2 (ja) * 2003-11-07 2009-09-30 花王株式会社 組換え微生物

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992019718A1 (en) * 1991-05-03 1992-11-12 Smithkline Beecham Corporation LOW TEMPERATURE-REGULATED PROMOTERS IN $i(E. COLI)
EP0691406A1 (de) * 1983-07-15 1996-01-10 Bio-Technology General Corporation Verfahren zur Herstellung von Superoxid dismutase durch rekombinante DNS Technologie
WO1996003521A1 (en) * 1994-07-21 1996-02-08 Yissum Research And Development Co. Vectors and transformed host cells for recombinant protein production at reduced temperatures
WO1998027220A1 (en) * 1996-12-19 1998-06-25 University Of Medicine And Dentistry Of New Jersey Method and constructs for inhibiting protein expression in bacteria

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0567575B1 (de) * 1991-01-14 1999-10-13 New York University Cytokin-induziertes protein, tsg-6, seine dna und verwendung
JP3469902B2 (ja) * 1995-12-27 2003-11-25 日本たばこ産業株式会社 低温誘導性プロモーター配列
WO1998042854A1 (en) * 1997-03-27 1998-10-01 The Board Of Trustees Of The Leland Stanford Junior University Functional genomic screen for rna regulatory sequences and interacting molecules
WO1999023216A2 (en) * 1997-11-03 1999-05-14 The Arizona Board Of Regents On Behalf Of The University Of Arizona Hyperthermic inducible expression vectors for gene therapy and methods of use thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0691406A1 (de) * 1983-07-15 1996-01-10 Bio-Technology General Corporation Verfahren zur Herstellung von Superoxid dismutase durch rekombinante DNS Technologie
WO1992019718A1 (en) * 1991-05-03 1992-11-12 Smithkline Beecham Corporation LOW TEMPERATURE-REGULATED PROMOTERS IN $i(E. COLI)
WO1996003521A1 (en) * 1994-07-21 1996-02-08 Yissum Research And Development Co. Vectors and transformed host cells for recombinant protein production at reduced temperatures
WO1998027220A1 (en) * 1996-12-19 1998-06-25 University Of Medicine And Dentistry Of New Jersey Method and constructs for inhibiting protein expression in bacteria

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BENTLEY W E ET AL: "Antisense RNA for manipulating cellular stresses in E. coli.", ABSTRACTS OF PAPERS AMERICAN CHEMICAL SOCIETY, vol. 213, no. 1-3, 1997, 213th National Meeting of the American Chemical Society;San Francisco, California, USA; April 13-17, 1997, pages BIOT 178, XP000926553, ISSN: 0065-7727 *

Also Published As

Publication number Publication date
WO2001031040A2 (de) 2001-05-03
DE19951765A1 (de) 2001-05-03
EP1224307A2 (de) 2002-07-24
WO2001031040A9 (de) 2002-09-06

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