WO2001027321A3 - Method for detecting differences in gene expression of wild type plants and genetically modified plants - Google Patents

Method for detecting differences in gene expression of wild type plants and genetically modified plants Download PDF

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Publication number
WO2001027321A3
WO2001027321A3 PCT/NL2000/000737 NL0000737W WO0127321A3 WO 2001027321 A3 WO2001027321 A3 WO 2001027321A3 NL 0000737 W NL0000737 W NL 0000737W WO 0127321 A3 WO0127321 A3 WO 0127321A3
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WO
WIPO (PCT)
Prior art keywords
wild type
plants
gene expression
plant
newly developed
Prior art date
Application number
PCT/NL2000/000737
Other languages
French (fr)
Other versions
WO2001027321A2 (en
Inventor
Der Krieken Wilhelmus Mari Van
Esther J Kok
Jaap Keijer
Original Assignee
Plant Res Int Bv
Krieken Wilhelmus Maria V D
Esther J Kok
Jaap Keijer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Res Int Bv, Krieken Wilhelmus Maria V D, Esther J Kok, Jaap Keijer filed Critical Plant Res Int Bv
Priority to AU11790/01A priority Critical patent/AU1179001A/en
Publication of WO2001027321A2 publication Critical patent/WO2001027321A2/en
Publication of WO2001027321A3 publication Critical patent/WO2001027321A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method for detecting differences in gene expression between wild type plants and newly developed plants, comprising of: a) providing at least one micro-array which is provided with a number of spots of target material; b) proividing at least one probe which comprises test material of a wild type plant, and a probe which comprises test material of a newly developed plant; c) placing on the micro-array the target material spotted thereon in contact with the probes; d) determining per spot whether hybridization of the probe of the wild type plant with the target material has taken place and determining per spot whether hybridization of the probe of the newly developed plant with the target material has taken place, in order to determine whether there is a difference in gene expression between the wild type plant and the newly developed plant, characterized in that the test material comes from plants which have been subjected to a treatment prior to isolation of the test material. With the method according to the invention it is possible to demonstrate in simple manner to what extent the gene expression of a newly developed plant differs, as a consequence of a worst case treatment, from the corresponding wild type plant.
PCT/NL2000/000737 1999-10-13 2000-10-13 Method for detecting differences in gene expression of wild type plants and genetically modified plants WO2001027321A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU11790/01A AU1179001A (en) 1999-10-13 2000-10-13 Method for detecting differences in gene expression between wild type plants andnewly developed plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL1013286 1999-10-13
NL1013286 1999-10-13

Publications (2)

Publication Number Publication Date
WO2001027321A2 WO2001027321A2 (en) 2001-04-19
WO2001027321A3 true WO2001027321A3 (en) 2001-12-27

Family

ID=19770047

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2000/000737 WO2001027321A2 (en) 1999-10-13 2000-10-13 Method for detecting differences in gene expression of wild type plants and genetically modified plants

Country Status (2)

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AU (1) AU1179001A (en)
WO (1) WO2001027321A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6960439B2 (en) 1999-06-28 2005-11-01 Source Precision Medicine, Inc. Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles
US6964850B2 (en) 2001-11-09 2005-11-15 Source Precision Medicine, Inc. Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000065039A2 (en) * 1999-04-28 2000-11-02 Syngenta Participations Ag Identification of dna control elements responsive to specific stimuli

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000065039A2 (en) * 1999-04-28 2000-11-02 Syngenta Participations Ag Identification of dna control elements responsive to specific stimuli

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DESPREZ T ET AL: "DIFFERENTIAL GENE EXPRESSION IN ARABIDOPSIS MONITORED USING CDNA ARRAYS", PLANT JOURNAL,BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD,GB, vol. 14, no. 5, June 1998 (1998-06-01), pages 643 - 652, XP000960485, ISSN: 0960-7412 *
LAZAROVA G I ET AL.: "Molecular analysis of PHYA in wild-type and phytochrome A-deficient mutants of tomato", THE PLANT JOURNAL, vol. 14, no. 6, 1998, pages 653 - 662, XP002171935 *
RUAN Y ET AL: "TOWARDS ARABIDOPSIS GENOME ANALYSIS: MONITORING EXPRESSION PROFILESOF 1400 GENES USING CDNA MICROARRAYS", PLANT JOURNAL,BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD,GB, vol. 15, no. 6, September 1998 (1998-09-01), pages 821 - 833, XP000960486, ISSN: 0960-7412 *
SCHENA M ET AL: "QUANTITATIVE MONITORING OF GENE EXPRESSION PATTERNS WITH A COMPLEMENTARY DNA MICROARRAY", SCIENCE,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,,US, vol. 270, no. 5235, 20 October 1995 (1995-10-20), pages 467 - 470, XP000644675, ISSN: 0036-8075 *
THOMPSON ANDREW J ET AL: "Molecular and genetic characterization of a novel pleiotropic tomato-ripening mutant", PLANT PHYSIOLOGY,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD,US, vol. 120, no. 2, June 1999 (1999-06-01), pages 383 - 389, XP002157274, ISSN: 0032-0889 *
VAN HAL N L W ET AL: "The application of DNA microarrays in gene expression analysis", JOURNAL OF BIOTECHNOLOGY,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM,NL, vol. 78, no. 3, March 2000 (2000-03-01), pages 271 - 280, XP004194231, ISSN: 0168-1656 *

Also Published As

Publication number Publication date
AU1179001A (en) 2001-04-23
WO2001027321A2 (en) 2001-04-19

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