WO2001025469A3 - Procede de preparation de steroides modifies par fermentation de levures - Google Patents

Procede de preparation de steroides modifies par fermentation de levures Download PDF

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Publication number
WO2001025469A3
WO2001025469A3 PCT/FR2000/002753 FR0002753W WO0125469A3 WO 2001025469 A3 WO2001025469 A3 WO 2001025469A3 FR 0002753 W FR0002753 W FR 0002753W WO 0125469 A3 WO0125469 A3 WO 0125469A3
Authority
WO
WIPO (PCT)
Prior art keywords
modified
steroids
yeast fermentation
hydroxylated
yeasts
Prior art date
Application number
PCT/FR2000/002753
Other languages
English (en)
Other versions
WO2001025469A2 (fr
Inventor
Gilles Cauet
Eric Degryse
Pedro Vico
Richard Lathe
Original Assignee
Transgene Sa
Gilles Cauet
Eric Degryse
Pedro Vico
Richard Lathe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgene Sa, Gilles Cauet, Eric Degryse, Pedro Vico, Richard Lathe filed Critical Transgene Sa
Priority to JP2001528620A priority Critical patent/JP2004500340A/ja
Priority to AU76726/00A priority patent/AU780186B2/en
Priority to EP00966279A priority patent/EP1218516A2/fr
Priority to CA002386419A priority patent/CA2386419A1/fr
Priority to US10/089,803 priority patent/US7033779B1/en
Publication of WO2001025469A2 publication Critical patent/WO2001025469A2/fr
Publication of WO2001025469A3 publication Critical patent/WO2001025469A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Steroid Compounds (AREA)

Abstract

La présente invention concerne un procédé de production de stéroïde hydroxylé et/ou acétylé comprenant les étapes selon lesquelles: on cultive des levures sur un milieu comprenant au moins un précurseur de tels stéroïdes hydroxylés et/ou acétylés, puis on isole les stéroïdes hydroxylés et/ou acétylés du milieu après bioconversion, ledit procédé étant caractérisé en ce que lesdites levures sont des levures transformées de façon à exprimer le produit du gène Cyp7b. L'invention concerne aussi les souches de levures modifiées.
PCT/FR2000/002753 1999-10-05 2000-10-04 Procede de preparation de steroides modifies par fermentation de levures WO2001025469A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2001528620A JP2004500340A (ja) 1999-10-05 2000-10-04 酵母発酵による修飾ステロイド類の製造方法
AU76726/00A AU780186B2 (en) 1999-10-05 2000-10-04 Method for preparing steroids modified by yeast fermentation
EP00966279A EP1218516A2 (fr) 1999-10-05 2000-10-04 Procede de preparation de steroides modifies par fermentation de levures
CA002386419A CA2386419A1 (fr) 1999-10-05 2000-10-04 Procede de preparation de steroides modifies par fermentation de levures
US10/089,803 US7033779B1 (en) 1999-10-05 2000-10-04 Method for preparing steroids modified by yeast fermentation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR99/12410 1999-10-05
FR9912410A FR2799206A1 (fr) 1999-10-05 1999-10-05 Procede de production de steroides modifies par fermentation de levures

Publications (2)

Publication Number Publication Date
WO2001025469A2 WO2001025469A2 (fr) 2001-04-12
WO2001025469A3 true WO2001025469A3 (fr) 2002-02-28

Family

ID=9550591

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2000/002753 WO2001025469A2 (fr) 1999-10-05 2000-10-04 Procede de preparation de steroides modifies par fermentation de levures

Country Status (7)

Country Link
US (1) US7033779B1 (fr)
EP (1) EP1218516A2 (fr)
JP (1) JP2004500340A (fr)
AU (1) AU780186B2 (fr)
CA (1) CA2386419A1 (fr)
FR (1) FR2799206A1 (fr)
WO (1) WO2001025469A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2812884B1 (fr) 2000-08-08 2002-11-08 Aventis Pharma Sa Levures modifiees et utilisations, notamment pour la production de derives steroidiens
FR2820145B1 (fr) 2001-01-31 2004-01-23 Aventis Pharma Sa Souche de levure produisant des steroides de facon autonome

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996012810A1 (fr) * 1994-10-19 1996-05-02 British Technology Group Proteines associees a l'hippocampe, leur codage par sequences adn et utilisation de ces proteines
WO1997037664A2 (fr) * 1996-04-09 1997-10-16 British Technology Group Ltd. Utilisation de steroides a substitution 7 alpha pour traiter les troubles neuropsychiatriques, immunitaires ou endocriniens
WO1999040203A1 (fr) * 1998-02-05 1999-08-12 Hoechst Marion Roussel Souches de levure ayant le gene atf2 interrompu et leurs applications

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996012810A1 (fr) * 1994-10-19 1996-05-02 British Technology Group Proteines associees a l'hippocampe, leur codage par sequences adn et utilisation de ces proteines
WO1997037664A2 (fr) * 1996-04-09 1997-10-16 British Technology Group Ltd. Utilisation de steroides a substitution 7 alpha pour traiter les troubles neuropsychiatriques, immunitaires ou endocriniens
WO1999040203A1 (fr) * 1998-02-05 1999-08-12 Hoechst Marion Roussel Souches de levure ayant le gene atf2 interrompu et leurs applications

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ATHENSTAEDT K ET AL.: "1-Acyldihydroxyacetone-phosphate reductase (Ayr1p) of the yeast Saccharomyces cerevisiae encoded by the open reading frame YIL124w is a major component of lipid particles", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 1, 7 January 2000 (2000-01-07), pages 235 - 240, XP002144501 *
IWASAKI M ET AL.: "Site-directed mutagenesis of mouse steroid 7-alpha-hydroxylase (cytochrome P-450-7alpha): Role of residue-209 in determining steroid-cytochrome P-450 interaction.", BIOCHEMICAL JOURNAL, vol. 291, no. 2, 1993, pages 569 - 573, XP000990764 *
KOSHCHEYENKO K A ET AL.: "Immobilization of living microbial cells and their application for steroid transformations.", ENZYME AND MICROBIAL TECHNOLOGY, vol. 5, no. 1, 1983, pages 14 - 21, XP000990744 *
NAGATA K ET AL.: "Rat testosterone 7alpha-hydroxylase. Isolation, sequence, and expression of cDNA and its developmental regulation and induction by 3-methylcholanthrene.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 262, no. 6, 25 February 1987 (1987-02-25), pages 2787 - 2793, XP002163977 *
ROSE K A ET AL.: "Cyp7b, a novel brain cytochrome P450, catalyzes the synthesis of neurosteroids 7.alpha.-hydroxy dehydroepiandrosterone and 7.alpha.-hydroxy pregnenolone", PROC. NAT'L. ACAD. SCI. USA, vol. 94, no. 10, 13 May 1997 (1997-05-13), pages 4925 - 4930, XP002163976 *
See also references of EP1218516A2 *
WARD O P ET AL.: "Reductive biotransformations of organic compounds by cells or enzymes of yeast;", ENZYME AND MICROBIAL TECHNOLOGY, vol. 12, no. 7, 1990, pages 482 - 493, XP000990784 *

Also Published As

Publication number Publication date
EP1218516A2 (fr) 2002-07-03
US7033779B1 (en) 2006-04-25
FR2799206A1 (fr) 2001-04-06
WO2001025469A2 (fr) 2001-04-12
CA2386419A1 (fr) 2001-04-12
JP2004500340A (ja) 2004-01-08
AU7672600A (en) 2001-05-10
AU780186B2 (en) 2005-03-03

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