WO2001025417A3 - Dna library and its use in methods of selecting and designing polypeptides - Google Patents

Dna library and its use in methods of selecting and designing polypeptides Download PDF

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Publication number
WO2001025417A3
WO2001025417A3 PCT/GB2000/003765 GB0003765W WO0125417A3 WO 2001025417 A3 WO2001025417 A3 WO 2001025417A3 GB 0003765 W GB0003765 W GB 0003765W WO 0125417 A3 WO0125417 A3 WO 0125417A3
Authority
WO
WIPO (PCT)
Prior art keywords
library
sequences
selecting
methods
sub
Prior art date
Application number
PCT/GB2000/003765
Other languages
French (fr)
Other versions
WO2001025417A2 (en
Inventor
Yen Choo
Aaron Klug
Original Assignee
Gendaq Ltd
Yen Choo
Aaron Klug
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9923327.2A external-priority patent/GB9923327D0/en
Priority claimed from GB0011068A external-priority patent/GB0011068D0/en
Priority claimed from GB0013106A external-priority patent/GB0013106D0/en
Application filed by Gendaq Ltd, Yen Choo, Aaron Klug filed Critical Gendaq Ltd
Priority to EP00964464A priority Critical patent/EP1230355A2/en
Priority to AU75398/00A priority patent/AU7539800A/en
Priority to CA002382541A priority patent/CA2382541A1/en
Publication of WO2001025417A2 publication Critical patent/WO2001025417A2/en
Priority to AU2001252428A priority patent/AU2001252428A1/en
Priority to PCT/GB2001/002017 priority patent/WO2001085780A2/en
Publication of WO2001025417A3 publication Critical patent/WO2001025417A3/en
Priority to US10/276,608 priority patent/US20040039175A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A library is provided of DNA sequences consisting of 4N sequences, where N is greater than or equal to three, each sequence varying from the other sequences by comprising a different one of the 4N possible permutations of a DNA sequence of length N, wherein the library of DNA sequences is immobilised on a solid substrate. Preferably, each sequence occupies a discrete position, and preferably, the library is arranged in two or more sub-libraries, preferably 4N sub-libraries, wherein for any one sub-library one base in the DNA sequence of length N is defined and the other N-1 bases are randomised. The library may be used in screening methods to identify and characterise zinc fingers having specificity for particular nucleotide sequences.
PCT/GB2000/003765 1999-10-01 2000-10-02 Dna library and its use in methods of selecting and designing polypeptides WO2001025417A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP00964464A EP1230355A2 (en) 1999-10-01 2000-10-02 Dna library and its use in methods of selecting and designing polypeptides
AU75398/00A AU7539800A (en) 1999-10-01 2000-10-02 Dna library
CA002382541A CA2382541A1 (en) 1999-10-01 2000-10-02 Dna library and its use in methods of selecting and designing polypeptides
AU2001252428A AU2001252428A1 (en) 2000-05-08 2001-05-08 Nucleic acid binding polypeptides
PCT/GB2001/002017 WO2001085780A2 (en) 2000-05-08 2001-05-08 Nucleic acid binding polypeptides
US10/276,608 US20040039175A1 (en) 2000-05-08 2002-11-07 Modulation of viral gene expression by engineered zinc finger proteins

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB9923327.2 1999-10-01
GBGB9923327.2A GB9923327D0 (en) 1999-10-01 1999-10-01 DNA library
GB0011068.4 2000-05-08
GB0011068A GB0011068D0 (en) 2000-05-08 2000-05-08 Molecules
GB0013106A GB0013106D0 (en) 2000-05-30 2000-05-30 Molecules
GB0013106.0 2000-05-30

Publications (2)

Publication Number Publication Date
WO2001025417A2 WO2001025417A2 (en) 2001-04-12
WO2001025417A3 true WO2001025417A3 (en) 2001-11-15

Family

ID=27255701

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/003765 WO2001025417A2 (en) 1999-10-01 2000-10-02 Dna library and its use in methods of selecting and designing polypeptides

Country Status (4)

Country Link
EP (1) EP1230355A2 (en)
AU (1) AU7539800A (en)
CA (1) CA2382541A1 (en)
WO (1) WO2001025417A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE513040T1 (en) * 2001-08-17 2011-07-15 Toolgen Inc ZINC FINGER DOMAIN LIBRARIES
EP1498486A4 (en) * 2002-03-29 2005-08-03 Prec System Science Co Ltd Nucleic acid library and protein library
CA2561565C (en) * 2004-04-08 2013-11-26 Sangamo Biosciences, Inc. Methods for repression of phospholamban gene and modulating cardiac contractility
WO2007136840A2 (en) * 2006-05-20 2007-11-29 Codon Devices, Inc. Nucleic acid library design and assembly
SG11202107720UA (en) 2019-01-31 2021-08-30 Agency Science Tech & Res Cnx/erp57 inhibitor for use in the treatment or prevention of cancer

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693463A (en) * 1991-06-27 1997-12-02 Genelabs Technologies, Inc. Method of ordering sequence binding preferences of a DNA-binding molecule
US6007988A (en) * 1994-08-20 1999-12-28 Medical Research Council Binding proteins for recognition of DNA

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693463A (en) * 1991-06-27 1997-12-02 Genelabs Technologies, Inc. Method of ordering sequence binding preferences of a DNA-binding molecule
US6007988A (en) * 1994-08-20 1999-12-28 Medical Research Council Binding proteins for recognition of DNA

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHOO YEN ET AL: "Selection of DNA binding sites for zinc fingers using rationally randomized DNA reveals coded interactions.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 91, no. 23, 1994, 1994, pages 11168 - 11172, XP002075339, ISSN: 0027-8424 *
ISALAN MARK ET AL: "Comprehensive DNA recognition through concerted interactions from adjacent zinc fingers.", BIOCHEMISTRY, vol. 37, no. 35, pages 12026 - 12033, XP002171310, ISSN: 0006-2960 *
JAMIESON ANDREW C ET AL: "A zinc finger directory for high-affinity DNA recognition.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 93, no. 23, 1996, 1996, pages 12834 - 12839, XP002167946, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
WO2001025417A2 (en) 2001-04-12
AU7539800A (en) 2001-05-10
EP1230355A2 (en) 2002-08-14
CA2382541A1 (en) 2001-04-12

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