WO2001021270A3 - Methods for producing recombinant proteins - Google Patents

Methods for producing recombinant proteins Download PDF

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Publication number
WO2001021270A3
WO2001021270A3 PCT/US2000/026005 US0026005W WO0121270A3 WO 2001021270 A3 WO2001021270 A3 WO 2001021270A3 US 0026005 W US0026005 W US 0026005W WO 0121270 A3 WO0121270 A3 WO 0121270A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
recombinant proteins
plant tissue
producing recombinant
recovering
Prior art date
Application number
PCT/US2000/026005
Other languages
French (fr)
Other versions
WO2001021270A2 (en
Inventor
Zivko Nikolov
Original Assignee
Prodigene Inc
Zivko Nikolov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prodigene Inc, Zivko Nikolov filed Critical Prodigene Inc
Priority to AU77090/00A priority Critical patent/AU7709000A/en
Priority to CA002384828A priority patent/CA2384828A1/en
Priority to EP00966802A priority patent/EP1272508A2/en
Publication of WO2001021270A2 publication Critical patent/WO2001021270A2/en
Publication of WO2001021270A3 publication Critical patent/WO2001021270A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Methods are provided for producing and recovering recombinant proteins from plant tissue. The methods of the invention find use in the commercial processing of grains, particularly in the wet-milling of corn kernels. The methods involve steeping plant tissue and recovering the recombinant proteins in steep water. The methods additionally involve optimizing nucleic acid constructs and plants for the recovery of recombinant proteins from plant tissue.
PCT/US2000/026005 1999-09-21 2000-09-21 Methods for producing recombinant proteins WO2001021270A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU77090/00A AU7709000A (en) 1999-09-21 2000-09-21 Methods for producing recombinant proteins
CA002384828A CA2384828A1 (en) 1999-09-21 2000-09-21 Methods for producing recombinant proteins
EP00966802A EP1272508A2 (en) 1999-09-21 2000-09-21 Methods for producing recombinant proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15492399P 1999-09-21 1999-09-21
US60/154,923 1999-09-21

Publications (2)

Publication Number Publication Date
WO2001021270A2 WO2001021270A2 (en) 2001-03-29
WO2001021270A3 true WO2001021270A3 (en) 2001-05-10

Family

ID=22553395

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/026005 WO2001021270A2 (en) 1999-09-21 2000-09-21 Methods for producing recombinant proteins

Country Status (4)

Country Link
EP (1) EP1272508A2 (en)
AU (1) AU7709000A (en)
CA (1) CA2384828A1 (en)
WO (1) WO2001021270A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003523721A (en) 1998-12-31 2003-08-12 カイロン コーポレイション Polynucleotides encoding antigenic HIVC-type polypeptides, polypeptides, and uses thereof
US7211659B2 (en) 2001-07-05 2007-05-01 Chiron Corporation Polynucleotides encoding antigenic HIV type C polypeptides, polypeptides and uses thereof
US20090137022A1 (en) * 2005-03-08 2009-05-28 Protech Research Pty Ltd Extracting and purifying beta 1,4-xylanase
MX2007014674A (en) * 2005-05-24 2008-03-07 Avestha Gengraine Tech Pvt Ltd A process comprising codon optimization for the production of recombinant activated human protein c for the treatment of sepsis.
CN106047885B (en) * 2015-09-14 2019-07-05 中国科学院烟台海岸带研究所 A kind of jerusalem artichoke anti insect gene and its expression vector establishment methods and applications
KR102086624B1 (en) * 2018-02-26 2020-03-10 중앙대학교 산학협력단 Transgenic tobacco for high expression of brazzein and method for producing thereof
KR102086625B1 (en) * 2018-08-24 2020-03-09 중앙대학교 산학협력단 Production method of brazzein from transgenic tobacco for high expression of brazzein
CN109479704B (en) * 2018-12-19 2020-12-25 上海市农业科学院 Method for saving premature flat peach embryo

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0321004A1 (en) * 1987-11-17 1989-06-21 Dorr-Oliver Incorporated A process for steeping cereals with a new enzyme preparation
WO1994008027A1 (en) * 1992-09-28 1994-04-14 Midwest Research Institute Fermentation of cellulose and hemicellulose in corn fiber and distillers dried grains with solubles to ethanol
WO1997004123A1 (en) * 1995-07-19 1997-02-06 Gel Tech Group Inc. Collagen compound production in plants
WO1997017453A1 (en) * 1995-11-06 1997-05-15 Prodigene Commercial production of aprotinin in plants

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0321004A1 (en) * 1987-11-17 1989-06-21 Dorr-Oliver Incorporated A process for steeping cereals with a new enzyme preparation
WO1994008027A1 (en) * 1992-09-28 1994-04-14 Midwest Research Institute Fermentation of cellulose and hemicellulose in corn fiber and distillers dried grains with solubles to ethanol
WO1997004123A1 (en) * 1995-07-19 1997-02-06 Gel Tech Group Inc. Collagen compound production in plants
WO1997017453A1 (en) * 1995-11-06 1997-05-15 Prodigene Commercial production of aprotinin in plants

Also Published As

Publication number Publication date
WO2001021270A2 (en) 2001-03-29
AU7709000A (en) 2001-04-24
CA2384828A1 (en) 2001-03-29
EP1272508A2 (en) 2003-01-08

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