WO2001020011A1 - Dna construct and its use - Google Patents
Dna construct and its use Download PDFInfo
- Publication number
- WO2001020011A1 WO2001020011A1 PCT/SE2000/001767 SE0001767W WO0120011A1 WO 2001020011 A1 WO2001020011 A1 WO 2001020011A1 SE 0001767 W SE0001767 W SE 0001767W WO 0120011 A1 WO0120011 A1 WO 0120011A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- oilseed plant
- xanthophyll
- dna construct
- peptide
- nucleotide sequence
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
Definitions
- the present invention relates to a new DNA construct for transformation into oilseed plants.
- the DNA construct comprises nucleotide sequences encoding peptides with enzyme activities necessary for the high-level production and esterification of keto group-containing xanthophylls in oilseed plants.
- Carotenoids are produced de novo by plants, fungi, algae and some bacteria. A number of biosynthetic steps are needed for the biological production of the carotenoids. There are two chemically different groups of carotenoids, namely carotenes containing only carbon and hydrogen molecules and xanthophylls containing oxygen in the molecule in addition to carbon and hydrogen.
- the xanthophylls and particularly astaxanthin (3,3'-dihydroxy- ⁇ - ⁇ -carotene-4,4'- dione), are often colored pigments and are used as such or as anti-oxidants.
- Carotenes are biological precursors for the production of the oxygen-containing xanthophylls. There are two types of enzymes responsible for the introduction of hydroxy groups and keto groups into the carotenes, namely hydroxylases and ketolases, respectively.
- keto group-containing xanthophyll astaxanthin which has keto and hydroxy groups, is biosynthetically produced from beta-carotene.
- Large-scale production of xanthophylles from natural sources is at present performed by AstaCarotene AB, Gustavsberg, Sweden, by cultivation of the alga Haematococcus pluvialis for the production of astaxanthin in esterified form.
- Oilseed plants have naturally ⁇ -carotene hydroxylases but lack ⁇ -carotene C-4-oxygenase enzymes or ketolases.
- the present invention provides DNA constructs enabling and promoting production of keto group containing xanthophylls, especially astaxanthin, in oilseed plants, such as rape, sunflower, soybean and mustard.
- the DNA construct is transformed into the oilseed plant cell for expression of a protein or fused protein which has an enzyme activity enabling keto group insertion into a carotene or hydroxy carotene for the biosynthetic production of a keto group containing xanthophyll, such as cantaxanthin ( ⁇ , ⁇ -carotene-4,4'- dione) and/or astaxanthin.
- cantaxanthin ⁇ , ⁇ -carotene-4,4'- dione
- the naturally occurring synthesis of carotenoids involves a number of enzymes, namely 1-D-deoxyxylulose 5-phosphate synthase, isopentenyl pyrophosphate:dimethylallyl pyrophosphate isomerase, geranylgeranyl pyrophosphate synthase, phytoene synthase, phytoene desaturase, zeta-carotene desaturase, lycopene beta- cyclase, ⁇ -carotene hydroxylase, and ⁇ -carotene C-4-oxygenase.
- enzymes namely 1-D-deoxyxylulose 5-phosphate synthase, isopentenyl pyrophosphate:dimethylallyl pyrophosphate isomerase, geranylgeranyl pyrophosphate synthase, phytoene synthase, phytoene desaturase, zeta-carotene desaturase, lycopene beta-
- Genes coding for peptides having these enzymatic activities may be inserted into the DNA construct of the invention, one or several per construct, to promote high-level production in the transgenic oilseed plant. In case only one enzyme coding gene is inserted per plant, two or more plants may be sexually interbred to produce plants containing all the desired enzyme activities.
- the present invention is directed to a DNA construct comprising in the 5' to 3' direction of transcription operably linked a promoter region directing transcription to the seed of an oilseed plant, a nucleotide sequence coding for at least one peptide with enzyme activity necessary for keto group containing xanthophyll production and esterification in an oilseed plant and a transcriptional termination region.
- the DNA construct additionally comprises between the promoter region and the nucleotide sequence coding for at least one peptide with enzyme activity a nucleotide sequence coding for a transit peptide directing the translated fusion polypeptide to the chloroplast of the oilseed plant.
- the DNA construct is preferably such that the promoter is a napin promoter, the peptide with enzyme activity necessary for keto group containing xanthophyll production is selected from the group consisting of peptides with 1-D-deoxyxylulose 5-phosphate synthase, isopentenyl pyrophosphate:dimethylallyl pyrophosphate isomerase, geranylgeranyl pyrophosphate synthase, phytoene synthase, phytoene desaturase, zeta-carotene desaturase, lycopene beta-cyclase, ⁇ -carotene hydroxylase, and ⁇ -carotene C-4-oxygenase activity.
- a nucleotide sequence coding for a peptide with acyl transferase activity may be included in the group.
- the nucleotide sequence coding for a peptide with enzyme activity is a nucleotide sequence coding for a N-terminally truncated ⁇ -carotene C-4-oxygenase gene from the alga Haematococcus pluvialis.
- the present invention is also directed to a transgenic oilseed plant cell comprising the DNA construct of the invention, and preferably the oilseed plant is selected from the group consisting of rape, sunflower, soybean and mustard.
- the invention is additionally directed to transgenic oilseed plant-produced xanthophyll, e.g. canthaxanthin and astaxanthin.
- a preferred aspect of the invention is directed to transgenic oilseed plant- produced astaxanthin esters.
- Fig.l illustrates the nucleotide sequence of the DNA construct comprising the napin promoter, the chloroplast localization signal, the N-terminally truncated ⁇ -carotene C-4-oxygenase gene and the termination sequence, and the deduced amino acid sequences of the transit peptide and the ⁇ -carotene C-4-oxygenase.
- the invention is illustrated by production of astaxanthin in the seed of oilseed rape.
- the astaxanthin produced in the seed of the transgenic plant is extracted as part of the extracted oil.
- transgenic plants are produced having a chimeric DNA construct that is genetically inherited and is able to produce astaxanthin.
- the nucleotide sequence of the chimeric DNA construct consist of four parts of different genetic origin namely: (1) a promoter, (2) a localization signal, (3) a ⁇ -carotene C-4- oxygenase coding region and (4) a termination sequence.
- the napin promoter directs transcription to the seed of oilseed rape (Stalberg et al 1996). This promoter was coupled to a localization signal similar but not identical to a transit peptide (TP) of Rbcsla (Krebbers, 1988) that directs the translated product of a fused gene to the chloroplast.
- TP transit peptide
- the promoter and the TP sequence were ligated to a part of the coding sequence of a ketolase gene BCK (Kajiwara et al. 1995). This enzyme oxygenates ⁇ -carotene to canthaxanthin, (Fraser et al. 1997).
- the chimeric DNA construct was then coupled to a suitable termination sequence, e.g. that of the Agrobacterium tumefaciens nopaline synthase gene (the nos 3' end)(Bevan et al. 1983), as illustrated in Fig.l. Cellular storage of Astaxantin
- an effective esterification of astaxanthin to fatty acids enables storage of the esterified molecules in triacylglycerol containing oleosomes.
- an acyl transferase can be claimed to be of fundamental importance for the process, as is proteins that can mediate transport of different forms of astaxanthin from the chloroplast to the vesicles.
- Napin promoter (GeneBank ACCESSION No. J02798) This promoter sequence, a 1145 base pair fragment including the 5' leader sequence has a unique Hindlll site at the 5' end. The 3' end was synthesized with an additionally 6 nucleotide BamHI site.
- BCK ⁇ -carotene C-4 oxygenase
- Genebank ACCESSION No. D45881 The BCK fragment was amplified by PCR including a 5' Xbal site and was ligated to the TP already described.
- the 5' primer (BCK1) used for PCR is homologous to the BCK sequence from nucleotide 264 and the 3' oligonucleotide (Ax40) ends with a stop codon and was synthesized with a Sad restriction site for cloning.
- the synthesized fragment was fused to the TP as shown in Fig 1.
- Sad 3 primer AX40 5 'CACCGAGCTCCATGACACTCTTGTGCAGA 3 ' Description of SEQ ID NO:l and SEQ ID NO:2
- the SEQ ID NO:l is a nucleotide sequence composed of the following features: Nucleotide No.
- the SEQ ID NO: 2 is a deduced amino acid sequence of the fusion protein of the transit peptide and the peptide with ⁇ -carotene C-4-oxygenase activity.
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- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
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- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU76936/00A AU7693600A (en) | 1999-09-17 | 2000-09-13 | Dna construct and its use |
CA002382444A CA2382444A1 (en) | 1999-09-17 | 2000-09-13 | Dna construct and its use |
JP2001523782A JP2003509057A (en) | 1999-09-17 | 2000-09-13 | DNA constructs and uses thereof |
EP00966616A EP1212444A1 (en) | 1999-09-17 | 2000-09-13 | Dna construct and its use |
NO20021305A NO20021305L (en) | 1999-09-17 | 2002-03-15 | DNA construction and its use |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9903336-7 | 1999-09-17 | ||
SE9903336A SE9903336D0 (en) | 1999-09-17 | 1999-09-17 | DNA construct and its use |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2001020011A1 true WO2001020011A1 (en) | 2001-03-22 |
Family
ID=20417035
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/SE2000/001767 WO2001020011A1 (en) | 1999-09-17 | 2000-09-13 | Dna construct and its use |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP1212444A1 (en) |
JP (1) | JP2003509057A (en) |
AU (1) | AU7693600A (en) |
CA (1) | CA2382444A1 (en) |
NO (1) | NO20021305L (en) |
SE (1) | SE9903336D0 (en) |
WO (1) | WO2001020011A1 (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004101797A1 (en) * | 2003-05-15 | 2004-11-25 | Icon Genetics Ag | Process of producing a plastid-targeted protein in plant cells |
EP1867327A1 (en) | 2006-06-16 | 2007-12-19 | Yamaha Hatsudoki Kabushiki Kaisha | Astaxanthin and esters thereof for protecting neurocytes and treating Parkinson's disease |
US7385123B2 (en) | 2002-08-20 | 2008-06-10 | Sungene Gmbh & Co. Kgaa | Process for preparing ketocarotenoids in genetically modified organisms |
US7541168B2 (en) | 2000-07-18 | 2009-06-02 | National Research Council Of Canada | Recombinant cyclopentanone monooxygenase [cpmo] |
US8691555B2 (en) | 2006-09-28 | 2014-04-08 | Dsm Ip Assests B.V. | Production of carotenoids in oleaginous yeast and fungi |
US9096909B2 (en) | 2009-07-23 | 2015-08-04 | Chromatin, Inc. | Sorghum centromere sequences and minichromosomes |
US9909130B2 (en) | 2005-03-18 | 2018-03-06 | Dsm Ip Assets B.V. | Production of carotenoids in oleaginous yeast and fungi |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
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WO1996013149A1 (en) * | 1994-10-28 | 1996-05-09 | Amoco Corporation | Enhanced carotenoid accumulation in storage organs of genetically engineered plants |
WO1998006862A1 (en) * | 1996-08-09 | 1998-02-19 | Calgene Llc | Methods for producing carotenoid compounds and speciality oils in plant seeds |
WO1998018910A1 (en) * | 1995-11-24 | 1998-05-07 | Yissum Research And Development Company Of The Hebrew University Of Jerusalem | Nucleic acid sequence encoding beta-c-4-oxygenase from haematococcus pluvialis for the biosynthesis of astaxanthin |
WO1998037874A1 (en) * | 1997-02-27 | 1998-09-03 | Astacarotene Ab | Oral preparation for the prophylactic and therapeutic treatment of helicobacter sp. infection |
WO1999007867A1 (en) * | 1997-08-08 | 1999-02-18 | Calgene Llc | Methods for producing carotenoid compounds, and speciality oils in plant seeds |
WO1999030701A1 (en) * | 1997-12-16 | 1999-06-24 | Astacarotene Ab | Method of the prophylactic treatment of mastitis |
-
1999
- 1999-09-17 SE SE9903336A patent/SE9903336D0/en unknown
-
2000
- 2000-09-13 AU AU76936/00A patent/AU7693600A/en not_active Abandoned
- 2000-09-13 EP EP00966616A patent/EP1212444A1/en not_active Withdrawn
- 2000-09-13 WO PCT/SE2000/001767 patent/WO2001020011A1/en not_active Application Discontinuation
- 2000-09-13 JP JP2001523782A patent/JP2003509057A/en active Pending
- 2000-09-13 CA CA002382444A patent/CA2382444A1/en not_active Abandoned
-
2002
- 2002-03-15 NO NO20021305A patent/NO20021305L/en not_active Application Discontinuation
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
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WO1996013149A1 (en) * | 1994-10-28 | 1996-05-09 | Amoco Corporation | Enhanced carotenoid accumulation in storage organs of genetically engineered plants |
WO1998018910A1 (en) * | 1995-11-24 | 1998-05-07 | Yissum Research And Development Company Of The Hebrew University Of Jerusalem | Nucleic acid sequence encoding beta-c-4-oxygenase from haematococcus pluvialis for the biosynthesis of astaxanthin |
WO1998006862A1 (en) * | 1996-08-09 | 1998-02-19 | Calgene Llc | Methods for producing carotenoid compounds and speciality oils in plant seeds |
WO1998037874A1 (en) * | 1997-02-27 | 1998-09-03 | Astacarotene Ab | Oral preparation for the prophylactic and therapeutic treatment of helicobacter sp. infection |
WO1999007867A1 (en) * | 1997-08-08 | 1999-02-18 | Calgene Llc | Methods for producing carotenoid compounds, and speciality oils in plant seeds |
WO1999030701A1 (en) * | 1997-12-16 | 1999-06-24 | Astacarotene Ab | Method of the prophylactic treatment of mastitis |
Non-Patent Citations (8)
Title |
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BISWAL B. ET AL.: "Carotenoid catabolism during leaf senescence and its control by light", JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B, vol. 30, 1995, pages 3 - 13, XP002908967 * |
DATABASE GENBANK [online] 10 September 1999 (1999-09-10), LINDEN H.: "Haematococcus pluvialis carotenoid hydroxylase mRNA", XP002908964, accession no. EMBL Database accession no. AF162276 * |
DATABASE GENBANK [online] 18 August 1998 (1998-08-18), SUN Z. ET AL.: "Haematococcus pluvialis isopentenyl pyrophosphate:dimethylallyl pyrophosphate isomerase (ipiHp1) mRNA", XP002908961, accession no. EMBL Database accession no. AF082325 * |
DATABASE GENBANK [online] 18 August 1998 (1998-08-18), SUN Z. ET AL.: "Haematococcus pluvialis isopentenyl pyrophosphate:dimethylallyl pyrophosphate isomerase (ipiHp2) mRNA", XP002908963, accession no. EMBL Database accession no. AF082326 * |
DATABASE GENBANK [online] 2 June 1998 (1998-06-02), HARKER M. ET AL.: "H.pluvialis mRNA for phyteone desaturase", XP002908960, accession no. EMBL Database accession no. X86783 * |
DATABASE GENBANK [online] 29 December 1995 (1995-12-29), KAJIWAREA S.: "Haematococcus pluvialis mRNA for beta-carotene ketolase", XP002908959, accession no. EMBL Database accession no. D45881 * |
DATABASE GENBANK [online] 30 November 1997 (1997-11-30), HARKER M. ET AL.: "H.pluvialis mRNA for beta-carotene C-4 oxygenase", XP002908958, accession no. EMBL Database accession no. X86782 * |
SUSAN BUDAVARI ET AL.: "The Merck Index", 1996, MERCK & CO., INC., NJ, XP002970607 * |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7541168B2 (en) | 2000-07-18 | 2009-06-02 | National Research Council Of Canada | Recombinant cyclopentanone monooxygenase [cpmo] |
US7385123B2 (en) | 2002-08-20 | 2008-06-10 | Sungene Gmbh & Co. Kgaa | Process for preparing ketocarotenoids in genetically modified organisms |
WO2004101797A1 (en) * | 2003-05-15 | 2004-11-25 | Icon Genetics Ag | Process of producing a plastid-targeted protein in plant cells |
AU2004238991B2 (en) * | 2003-05-15 | 2010-04-22 | Icon Genetics Gmbh | Process of producing a plastid-targeted protein in plant cells |
US9909130B2 (en) | 2005-03-18 | 2018-03-06 | Dsm Ip Assets B.V. | Production of carotenoids in oleaginous yeast and fungi |
EP1867327A1 (en) | 2006-06-16 | 2007-12-19 | Yamaha Hatsudoki Kabushiki Kaisha | Astaxanthin and esters thereof for protecting neurocytes and treating Parkinson's disease |
US8691555B2 (en) | 2006-09-28 | 2014-04-08 | Dsm Ip Assests B.V. | Production of carotenoids in oleaginous yeast and fungi |
US9297031B2 (en) | 2006-09-28 | 2016-03-29 | Dsm Ip Assets B.V. | Production of carotenoids in oleaginous yeast and fungi |
US9096909B2 (en) | 2009-07-23 | 2015-08-04 | Chromatin, Inc. | Sorghum centromere sequences and minichromosomes |
Also Published As
Publication number | Publication date |
---|---|
NO20021305D0 (en) | 2002-03-15 |
NO20021305L (en) | 2002-05-15 |
CA2382444A1 (en) | 2001-03-22 |
AU7693600A (en) | 2001-04-17 |
SE9903336D0 (en) | 1999-09-17 |
EP1212444A1 (en) | 2002-06-12 |
JP2003509057A (en) | 2003-03-11 |
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