WO2001016144A3 - Plant derived calcium-dependent calmodulin-binding polypeptides and polynucleotides encoding same - Google Patents

Plant derived calcium-dependent calmodulin-binding polypeptides and polynucleotides encoding same Download PDF

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Publication number
WO2001016144A3
WO2001016144A3 PCT/IL2000/000511 IL0000511W WO0116144A3 WO 2001016144 A3 WO2001016144 A3 WO 2001016144A3 IL 0000511 W IL0000511 W IL 0000511W WO 0116144 A3 WO0116144 A3 WO 0116144A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotides encoding
binding polypeptides
plant derived
encoding same
derived calcium
Prior art date
Application number
PCT/IL2000/000511
Other languages
French (fr)
Other versions
WO2001016144A2 (en
Inventor
Hillel Fromm
Tzahi Arazi
Original Assignee
Yeda Res & Dev
Hillel Fromm
Tzahi Arazi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeda Res & Dev, Hillel Fromm, Tzahi Arazi filed Critical Yeda Res & Dev
Priority to AU67225/00A priority Critical patent/AU6722500A/en
Publication of WO2001016144A2 publication Critical patent/WO2001016144A2/en
Publication of WO2001016144A3 publication Critical patent/WO2001016144A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8259—Phytoremediation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance

Abstract

A method of modulating a tolerance of a plant to a metal cation is disclosed. The method is effected by overexpressing in the plant a recombinant protein which modulates uptake and concentration of the metal cation within the plant cells.
PCT/IL2000/000511 1999-08-29 2000-08-27 Plant derived calcium-dependent calmodulin-binding polypeptides and polynucleotides encoding same WO2001016144A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU67225/00A AU6722500A (en) 1999-08-29 2000-08-27 Plant derived calcium-dependent calmodulin-binding polypeptides and polynucleotides encoding same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL13163399A IL131633A0 (en) 1999-08-29 1999-08-29 Plant derived calcium-dependent calmodulin-binding polypeptides and polynucleotides encoding same
IL131633 1999-08-29

Publications (2)

Publication Number Publication Date
WO2001016144A2 WO2001016144A2 (en) 2001-03-08
WO2001016144A3 true WO2001016144A3 (en) 2001-10-18

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2000/000511 WO2001016144A2 (en) 1999-08-29 2000-08-27 Plant derived calcium-dependent calmodulin-binding polypeptides and polynucleotides encoding same

Country Status (3)

Country Link
AU (1) AU6722500A (en)
IL (1) IL131633A0 (en)
WO (1) WO2001016144A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6730832B1 (en) 2001-09-10 2004-05-04 Luis Mayan Dominguez High threonine producing lines of Nicotiana tobacum and methods for producing
US8716571B2 (en) 2011-09-21 2014-05-06 Reynolds Technologies, Inc. Tobacco having reduced amounts of amino acids and methods for producing such lines
US9137958B2 (en) 2012-02-08 2015-09-22 Reynolds Technologies, Inc. Tobacco having altered amounts of environmental contaminants
CN104470353B (en) * 2012-02-08 2018-02-13 雷诺兹科技股份有限公司 The tobacco of environmental contaminants with variable and the method for manufacturing this strain

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5608145A (en) * 1994-08-23 1997-03-04 The Regents Of The University Of California High-affinity potassium uptake transporter from higher plants
WO1997035968A1 (en) * 1996-03-28 1997-10-02 Washington State University Research Foundation Compositions and methods for production of male-sterile plants
US5846821A (en) * 1996-05-29 1998-12-08 Trustees Of Dartmouth College Metal-regulated transporters and uses therefor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5608145A (en) * 1994-08-23 1997-03-04 The Regents Of The University Of California High-affinity potassium uptake transporter from higher plants
WO1997035968A1 (en) * 1996-03-28 1997-10-02 Washington State University Research Foundation Compositions and methods for production of male-sterile plants
US5846821A (en) * 1996-05-29 1998-12-08 Trustees Of Dartmouth College Metal-regulated transporters and uses therefor

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
APSE ET AL.: "Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in arabidopsis", SCIENCE, vol. 285, 20 August 1999 (1999-08-20), pages 1256 - 1258, XP002940319 *
ARAZI ET AL., ALTMAN ET AL. EDS.: "Characterization of a novel family of calmodulin-binding plasma membrane channel-like proteins", 1999, PLANT BIOTECHNOLOGY AND IN VITRO BIOLOGY IN THE 21ST CENTURY, XP002940323 *
ARAZI ET AL.: "A tobacco plasma membrane calmodulin-binding transporter confers Ni2+ tolerance and Pb2+ hypersensitivity in transgenic plants", PLANT J., vol. 20, no. 2, October 1999 (1999-10-01), pages 171 - 182, XP002940324 *
ARAZI ET AL.: "Plant channel-like calcium/calmodulin-binding protein containing a putative cGMP binding site: Mapping of the CaM binding domain, subcellular localization and expression in transgenic plants", PLANT PHYSIOL., vol. 114, no. 3, SUPPL., August 1997 (1997-08-01), pages 197, XP002940318 *
DATABASE GENBANK [online] July 1998 (1998-07-01), ARAZI ET AL.: "Nicotiana tabacum cyclic nucleotide-gated calmodulin-binding ion channel (CBP4) mRNA, complete cds", XP002940321, Database accession no. AF079872 *
DATABASE GENBANK [online] July 1998 (1998-07-01), ARAZI ET AL.: "Nicotiana tabacum cyclic nucleotide-gated calmodulin-binding ion channel (CBP7) mRNA, complete cds", XP002940320, Database accession no. AF079871 *
KOHLER ET AL.: "Characterization of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in arabidopsis thaliana", PLANT J., vol. 18, no. 1, April 1999 (1999-04-01), pages 97 - 104, XP002940322 *
PLANT J., vol. 20, no. 2, 1999, pages 171 - 182 *

Also Published As

Publication number Publication date
IL131633A0 (en) 2001-01-28
AU6722500A (en) 2001-03-26
WO2001016144A2 (en) 2001-03-08

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