WO2001009375A3 - Inducible screen for drug discovery - Google Patents

Inducible screen for drug discovery Download PDF

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Publication number
WO2001009375A3
WO2001009375A3 PCT/GB2000/002932 GB0002932W WO0109375A3 WO 2001009375 A3 WO2001009375 A3 WO 2001009375A3 GB 0002932 W GB0002932 W GB 0002932W WO 0109375 A3 WO0109375 A3 WO 0109375A3
Authority
WO
WIPO (PCT)
Prior art keywords
coding sequence
cells
nitric oxide
inducible
screen
Prior art date
Application number
PCT/GB2000/002932
Other languages
French (fr)
Other versions
WO2001009375A2 (en
Inventor
Ian George Charles
Weiming Xu
Lizhi Liu
Original Assignee
Univ London
Ian George Charles
Weiming Xu
Lizhi Liu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9918077.0A external-priority patent/GB9918077D0/en
Priority claimed from GB0016171A external-priority patent/GB0016171D0/en
Application filed by Univ London, Ian George Charles, Weiming Xu, Lizhi Liu filed Critical Univ London
Priority to EP00951686A priority Critical patent/EP1203098A2/en
Priority to AU64546/00A priority patent/AU6454600A/en
Priority to GB0204762A priority patent/GB2371547A/en
Priority to CA002380323A priority patent/CA2380323A1/en
Publication of WO2001009375A2 publication Critical patent/WO2001009375A2/en
Publication of WO2001009375A3 publication Critical patent/WO2001009375A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for identifying a polynucleotide, the expression of which is modulated in the presence of nitric oxide (NO), which method comprises: (i) providing an mRNA or cDNA population from cells which contain a polynucleotide construct, which construct comprises: (a) a promoter operably linked to a coding sequence, wherein the promoter is responsive to ecdysone or an analog thereof and the coding sequence encodes a nitric oxide synthase (NOS) or a functional variant thereof; or (b) a promoter operably linked to one or more tetracycline operator site sequences and a coding sequence in that order, wherein the coding sequence encodes a nitric oxide synthase (NOS) or a functional variant thereof; (ii) providing an mRNA or cDNA population from cells as defined in step (i), said cells having been contacted with ecdysone or an analog thereof; and (iii) comparing the populations of steps (i) and (ii), thereby to determine which polynucleotides show modulated expression in the presence of NO.
PCT/GB2000/002932 1999-07-30 2000-07-28 Inducible screen for drug discovery WO2001009375A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00951686A EP1203098A2 (en) 1999-07-30 2000-07-28 Inducible screen for drug discovery
AU64546/00A AU6454600A (en) 1999-07-30 2000-07-28 Inducible screen for drug discovery
GB0204762A GB2371547A (en) 1999-07-30 2000-07-28 Inducible screen for drug discovery
CA002380323A CA2380323A1 (en) 1999-07-30 2000-07-28 Inducible screen for drug discovery

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB9918077.0 1999-07-30
GBGB9918077.0A GB9918077D0 (en) 1999-07-30 1999-07-30 Inducible screen for drug discovery
GB0016171A GB0016171D0 (en) 2000-06-30 2000-06-30 Inducible screen for drug discovery
GB0016171.1 2000-06-30

Publications (2)

Publication Number Publication Date
WO2001009375A2 WO2001009375A2 (en) 2001-02-08
WO2001009375A3 true WO2001009375A3 (en) 2001-05-25

Family

ID=26244580

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/002932 WO2001009375A2 (en) 1999-07-30 2000-07-28 Inducible screen for drug discovery

Country Status (5)

Country Link
EP (1) EP1203098A2 (en)
AU (1) AU6454600A (en)
CA (1) CA2380323A1 (en)
GB (1) GB2371547A (en)
WO (1) WO2001009375A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004017950A2 (en) * 2002-08-22 2004-03-04 Piramed Limited Phosphadidylinositol 3,5-biphosphate inhibitors as anti-viral agents

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759836A (en) * 1995-03-27 1998-06-02 Hospital For Joint Diseases Osteoarthritis-associated inducable isoform of nitric oxide synthetase
WO1998026066A1 (en) * 1996-12-09 1998-06-18 Ariad Gene Therapeutics, Inc. Expression of proteins for treating asthma via ligand mediated activation of their encoding genes
WO1999011620A1 (en) * 1997-08-28 1999-03-11 Pfizer Products Inc. Branched alkoxy-subsituted 2-aminopyridines as nos inhibitors
US5908756A (en) * 1996-08-30 1999-06-01 Johns Hopkins University Protein inhibitor of neuronal nitric oxide synthase
WO1999033971A1 (en) * 1997-12-31 1999-07-08 Medical College Of Georgia Research Institute, Inc. Oligomers that bind to ku protein

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE263838T1 (en) * 1991-02-26 2004-04-15 Astrazeneca Ab VECTOR
US5654168A (en) * 1994-07-01 1997-08-05 Basf Aktiengesellschaft Tetracycline-inducible transcriptional activator and tetracycline-regulated transcription units
WO1994029442A2 (en) * 1993-06-14 1994-12-22 Basf Aktiengesellschaft Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759836A (en) * 1995-03-27 1998-06-02 Hospital For Joint Diseases Osteoarthritis-associated inducable isoform of nitric oxide synthetase
US5908756A (en) * 1996-08-30 1999-06-01 Johns Hopkins University Protein inhibitor of neuronal nitric oxide synthase
WO1998026066A1 (en) * 1996-12-09 1998-06-18 Ariad Gene Therapeutics, Inc. Expression of proteins for treating asthma via ligand mediated activation of their encoding genes
WO1999011620A1 (en) * 1997-08-28 1999-03-11 Pfizer Products Inc. Branched alkoxy-subsituted 2-aminopyridines as nos inhibitors
WO1999033971A1 (en) * 1997-12-31 1999-07-08 Medical College Of Georgia Research Institute, Inc. Oligomers that bind to ku protein

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
AIDS (LONDON), vol. 12, no. 8, 28 May 1998 (1998-05-28), pages 865 - 872, ISSN: 0269-9370 *
CHRISTODOULOPOULOS GARYFALLIA ET AL: "Potentiation of chlorambucil cytotoxicity in B-cell chronic lymphocytic leukemia by inhibition of DNA-dependent protein kinase activity using wortmannin.", CANCER RESEARCH, vol. 58, no. 9, 1 May 1998 (1998-05-01), pages 1789 - 1792, XP002161828, ISSN: 0008-5472 *
DANIEL RENE ET AL: "A role for DNA-PK in retroviral DNA integration.", SCIENCE (WASHINGTON D C), vol. 284, no. 5414, 23 April 1999 (1999-04-23), pages 644 - 647, XP002161829, ISSN: 0036-8075 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 23 November 1998 (1998-11-23), HOSOI YOSHIO ET AL: "A phosphatidylinositol 3-kinase inhibitor wortmannin induces radioresistant DNA synthesis and sensitizes cells to bleomycin and ionizing radiation.", XP002161830, Database accession no. PREV199900006904 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 28 May 1998 (1998-05-28), DEL REAL GUSTAVO ET AL: "Suppression of HIV-1 infection in linomide-treated SCID-hu-PBL mice.", XP002161831, Database accession no. PREV199800315674 *
INTERNATIONAL JOURNAL OF CANCER, vol. 78, no. 5, 23 November 1998 (1998-11-23), pages 642 - 647, ISSN: 0020-7136 *
NO ET AL: "ECDYSONE-INDUCIBLE EXPRESSION IN MAMMALIAN CELLS AND TRANSGENIC MICE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, INDIA. SECTION A, PHYSICAL SCIENCES,IN,NATIONAL ACADEMY OF SCIENCE, ALLAHABAD, vol. 93, 1996, pages 3346 - 3351, XP002136440, ISSN: 0369-8203 *

Also Published As

Publication number Publication date
GB0204762D0 (en) 2002-04-17
CA2380323A1 (en) 2001-02-08
AU6454600A (en) 2001-02-19
WO2001009375A2 (en) 2001-02-08
EP1203098A2 (en) 2002-05-08
GB2371547A (en) 2002-07-31

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