GB2371547A - Inducible screen for drug discovery - Google Patents
Inducible screen for drug discovery Download PDFInfo
- Publication number
- GB2371547A GB2371547A GB0204762A GB0204762A GB2371547A GB 2371547 A GB2371547 A GB 2371547A GB 0204762 A GB0204762 A GB 0204762A GB 0204762 A GB0204762 A GB 0204762A GB 2371547 A GB2371547 A GB 2371547A
- Authority
- GB
- United Kingdom
- Prior art keywords
- inducible
- screen
- drug discovery
- vol
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000001939 inductive effect Effects 0.000 title description 3
- 238000007876 drug discovery Methods 0.000 title description 2
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 8
- 108091026890 Coding region Proteins 0.000 description 4
- 102000008299 Nitric Oxide Synthase Human genes 0.000 description 4
- 108010021487 Nitric Oxide Synthase Proteins 0.000 description 4
- 108091033319 polynucleotide Proteins 0.000 description 3
- 102000040430 polynucleotide Human genes 0.000 description 3
- 239000002157 polynucleotide Substances 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- UPEZCKBFRMILAV-JNEQICEOSA-N Ecdysone Natural products O=C1[C@H]2[C@@](C)([C@@H]3C([C@@]4(O)[C@@](C)([C@H]([C@H]([C@@H](O)CCC(O)(C)C)C)CC4)CC3)=C1)C[C@H](O)[C@H](O)C2 UPEZCKBFRMILAV-JNEQICEOSA-N 0.000 description 1
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 1
- 108091007960 PI3Ks Proteins 0.000 description 1
- 102000003993 Phosphatidylinositol 3-kinases Human genes 0.000 description 1
- 108090000430 Phosphatidylinositol 3-kinases Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- UPEZCKBFRMILAV-UHFFFAOYSA-N alpha-Ecdysone Natural products C1C(O)C(O)CC2(C)C(CCC3(C(C(C(O)CCC(C)(C)O)C)CCC33O)C)C3=CC(=O)C21 UPEZCKBFRMILAV-UHFFFAOYSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- UPEZCKBFRMILAV-JMZLNJERSA-N ecdysone Chemical compound C1[C@@H](O)[C@@H](O)C[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@@H]([C@H](O)CCC(C)(C)O)C)CC[C@]33O)C)C3=CC(=O)[C@@H]21 UPEZCKBFRMILAV-JMZLNJERSA-N 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
(12) UK Patent Application (19) GO (11) 2 37 1 547 (13) A (43) Date of
Printing by UK Office 31.07.2002 (21) Application No 0204762.9 (51) INTCL7 C12Q 1/68 // A61K 38/00, C07K 14/47, C12N 5/10
(22) Date of Filing 28.07.2000 15/63 (30) Priority Data (52) UK CL (Edition T) (31) 9918077 (32) 30.07.1999 (33) GB C3H HB7E H646 H650 H655 H656 H719
(31) 0016171 (32) 30.06.2000 U1S S1313
(86) International Application Data (56) Documents Cited by ISA PCT/GB00/02932 En 28.07.2000 WO 99/33971 A WO 99/11620 A (87) International Publication Data US 5908756 A US 5840521 A WO01/09375 En 08. 02.2001 US 5759836 A US 5654168 A Cancer Res., Vol.58, 1998, Christodoulopoulos, G. et al., "Potentiation of ehlorambucil cytotoxicrty. ..", (71) Applicant(s) pp.1789-1792.
University College London Int. J. Cancer, Vol.78, 1998, Hosoi, Y. et al., "A (Incorporated in the United Kingdom) phosphatidylinositol 3-kinase ", pp.642 647.
Gower Street, LONDON, WC1E 9BT, Unrted Kingdom AlDS(London), Vol.12, 1998, Del Real, G. et al., "Suppression of HIV-1...", pp.865 872.
(72) Inventor(s) Science, Vol.284, 1999, Daniel, R. et al., "A role for lan George Charles DNA-PK ", pp.644 647.
Weiming Xu Lizhi Liu (56) and (58) continued overleaf (74) continued overleaf (54) Abstract Title Inducible screen for drug discovery (57) A method for identifying a polynucleotide, the expression of which is modulated in the presence of nitric oxide (NO), which method comprises: (i) providing an mRNA or cDNA population from cells which contain a polynucleotide construct, which construct comprises: (a) a promoter operably linked to a coding sequence, wherein the promoter is responsive to ecdysone or an analog thereof and the coding sequence encodes a nitric oxide synthase (NOS) or a functional variant thereof: or (b) a promoter operably linked to one or more tetracycline operator site sequences and a coding sequence in that order, wherein the coding sequence encodes a nitric oxide synthase (NOS) or a functional variant thereof: (ii) providing an mRNA or cDNA population from cells as defined in step (i), said cells having been contacted with cedysone or an analog thereof: and (iii) comparing the populations of steps (i) and (ii), thereby to determine which polynucleotides show modulated expression in the presence of NO.
G) W CD n D
174) Agent and/or Address for Service (56) cant J A Kemp & Co. RN.A.S., Vol.93, 1996, No, et al., "Ecdysone-inducible 14 South Square, Gray's Inn, LONDON, WC1R 5JJ, expression ", pp.3346-3351.
United Kingdom Circulation, Vol.98, 1998, Iwashina, M. et al., "Transfection of inducible nitric oxide...", pp.1212-1218. Circulation, Vol.95, 1997, Kawaguchi, H. et al., "In viva transfection of...", pp.24412447.
Arterio. Thromb. Vasc. Biol., Vol., 17, 1997, Kullo, l. J. et al., "Expression and function of recombinant...", pp.2405-2412 Circ. Res., Vol. 80, 1997, Chen, A. F. Y. et al., "Expression and function of recombinant.. .", pp.327-335 (58) Field of Search by ISA
INT CL7 C12N, C12Q
Online: EPO-lnternal, WPI, PAJ, CHEM ABS, MEDUNE, EMBASE, BIOSIS
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9918077.0A GB9918077D0 (en) | 1999-07-30 | 1999-07-30 | Inducible screen for drug discovery |
GB0016171A GB0016171D0 (en) | 2000-06-30 | 2000-06-30 | Inducible screen for drug discovery |
PCT/GB2000/002932 WO2001009375A2 (en) | 1999-07-30 | 2000-07-28 | Inducible screen for drug discovery |
Publications (2)
Publication Number | Publication Date |
---|---|
GB0204762D0 GB0204762D0 (en) | 2002-04-17 |
GB2371547A true GB2371547A (en) | 2002-07-31 |
Family
ID=26244580
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB0204762A Withdrawn GB2371547A (en) | 1999-07-30 | 2000-07-28 | Inducible screen for drug discovery |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1203098A2 (en) |
AU (1) | AU6454600A (en) |
CA (1) | CA2380323A1 (en) |
GB (1) | GB2371547A (en) |
WO (1) | WO2001009375A2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004017950A2 (en) * | 2002-08-22 | 2004-03-04 | Piramed Limited | Phosphadidylinositol 3,5-biphosphate inhibitors as anti-viral agents |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5654168A (en) * | 1994-07-01 | 1997-08-05 | Basf Aktiengesellschaft | Tetracycline-inducible transcriptional activator and tetracycline-regulated transcription units |
US5759836A (en) * | 1995-03-27 | 1998-06-02 | Hospital For Joint Diseases | Osteoarthritis-associated inducable isoform of nitric oxide synthetase |
WO1998026066A1 (en) * | 1996-12-09 | 1998-06-18 | Ariad Gene Therapeutics, Inc. | Expression of proteins for treating asthma via ligand mediated activation of their encoding genes |
US5840521A (en) * | 1991-02-26 | 1998-11-24 | Imperial Chemical Industriles Plc | Expression vector containing an inducible selection gene system |
WO1999011620A1 (en) * | 1997-08-28 | 1999-03-11 | Pfizer Products Inc. | Branched alkoxy-subsituted 2-aminopyridines as nos inhibitors |
US5908756A (en) * | 1996-08-30 | 1999-06-01 | Johns Hopkins University | Protein inhibitor of neuronal nitric oxide synthase |
WO1999033971A1 (en) * | 1997-12-31 | 1999-07-08 | Medical College Of Georgia Research Institute, Inc. | Oligomers that bind to ku protein |
US5922927A (en) * | 1993-06-14 | 1999-07-13 | Basf Aktiengesellschaft | Methods for producing tetracycline-regulated transgenic mice |
-
2000
- 2000-07-28 WO PCT/GB2000/002932 patent/WO2001009375A2/en not_active Application Discontinuation
- 2000-07-28 AU AU64546/00A patent/AU6454600A/en not_active Abandoned
- 2000-07-28 CA CA002380323A patent/CA2380323A1/en not_active Abandoned
- 2000-07-28 EP EP00951686A patent/EP1203098A2/en not_active Withdrawn
- 2000-07-28 GB GB0204762A patent/GB2371547A/en not_active Withdrawn
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5840521A (en) * | 1991-02-26 | 1998-11-24 | Imperial Chemical Industriles Plc | Expression vector containing an inducible selection gene system |
US5922927A (en) * | 1993-06-14 | 1999-07-13 | Basf Aktiengesellschaft | Methods for producing tetracycline-regulated transgenic mice |
US5654168A (en) * | 1994-07-01 | 1997-08-05 | Basf Aktiengesellschaft | Tetracycline-inducible transcriptional activator and tetracycline-regulated transcription units |
US5759836A (en) * | 1995-03-27 | 1998-06-02 | Hospital For Joint Diseases | Osteoarthritis-associated inducable isoform of nitric oxide synthetase |
US5908756A (en) * | 1996-08-30 | 1999-06-01 | Johns Hopkins University | Protein inhibitor of neuronal nitric oxide synthase |
WO1998026066A1 (en) * | 1996-12-09 | 1998-06-18 | Ariad Gene Therapeutics, Inc. | Expression of proteins for treating asthma via ligand mediated activation of their encoding genes |
WO1999011620A1 (en) * | 1997-08-28 | 1999-03-11 | Pfizer Products Inc. | Branched alkoxy-subsituted 2-aminopyridines as nos inhibitors |
WO1999033971A1 (en) * | 1997-12-31 | 1999-07-08 | Medical College Of Georgia Research Institute, Inc. | Oligomers that bind to ku protein |
Non-Patent Citations (9)
Title |
---|
AIDS(London), Vol.12, 1998, Del Real, G. et al., "Suppression of HIV-1...", pp.865-872. * |
Arterio. Thromb. Vasc. Biol., Vol., 17, 1997, Kullo, I. J. et al., "Expression and function of recombinant...", pp.2405-2412 * |
Cancer Res., Vol.58, 1998, Christodoulopoulos, G. et al., "Potentiation of chlorambucil cytotoxicity...", pp.1789-1792. * |
Circ. Res., Vol.80, 1997, Chen, A. F. Y. et al., "Expression and function of recombinant...", pp.327-335 * |
Circulation, Vol.95, 1997, Kawaguchi, H. et al., "In vivo transfection of...", pp.2441-2447. * |
Circulation, Vol.98, 1998, Iwashina, M. et al., "Transfection of inducible nitric oxide...", pp.1212-1218. * |
Int. J. Cancer, Vol.78, 1998, Hosoi, Y. et al., "A phosphatidylinositol 3-kinase...", pp.642-647. * |
P.N.A.S., Vol.93, 1996, No, et al., "Ecdysone-inducible expression...", pp.3346-3351. * |
Science, Vol.284, 1999, Daniel, R. et al., "A role for DNA-PK...", pp.644-647. * |
Also Published As
Publication number | Publication date |
---|---|
GB0204762D0 (en) | 2002-04-17 |
CA2380323A1 (en) | 2001-02-08 |
AU6454600A (en) | 2001-02-19 |
WO2001009375A3 (en) | 2001-05-25 |
WO2001009375A2 (en) | 2001-02-08 |
EP1203098A2 (en) | 2002-05-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |