GB2371547A - Inducible screen for drug discovery - Google Patents

Inducible screen for drug discovery Download PDF

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Publication number
GB2371547A
GB2371547A GB0204762A GB0204762A GB2371547A GB 2371547 A GB2371547 A GB 2371547A GB 0204762 A GB0204762 A GB 0204762A GB 0204762 A GB0204762 A GB 0204762A GB 2371547 A GB2371547 A GB 2371547A
Authority
GB
United Kingdom
Prior art keywords
inducible
screen
drug discovery
vol
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0204762A
Other versions
GB0204762D0 (en
Inventor
Ian George Charles
Weiming Xu
Lizhi Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University College London
Original Assignee
University College London
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9918077.0A external-priority patent/GB9918077D0/en
Priority claimed from GB0016171A external-priority patent/GB0016171D0/en
Application filed by University College London filed Critical University College London
Publication of GB0204762D0 publication Critical patent/GB0204762D0/en
Publication of GB2371547A publication Critical patent/GB2371547A/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Description

(12) UK Patent Application (19) GO (11) 2 37 1 547 (13) A (43) Date of
Printing by UK Office 31.07.2002 (21) Application No 0204762.9 (51) INTCL7 C12Q 1/68 // A61K 38/00, C07K 14/47, C12N 5/10
(22) Date of Filing 28.07.2000 15/63 (30) Priority Data (52) UK CL (Edition T) (31) 9918077 (32) 30.07.1999 (33) GB C3H HB7E H646 H650 H655 H656 H719
(31) 0016171 (32) 30.06.2000 U1S S1313
(86) International Application Data (56) Documents Cited by ISA PCT/GB00/02932 En 28.07.2000 WO 99/33971 A WO 99/11620 A (87) International Publication Data US 5908756 A US 5840521 A WO01/09375 En 08. 02.2001 US 5759836 A US 5654168 A Cancer Res., Vol.58, 1998, Christodoulopoulos, G. et al., "Potentiation of ehlorambucil cytotoxicrty. ..", (71) Applicant(s) pp.1789-1792.
University College London Int. J. Cancer, Vol.78, 1998, Hosoi, Y. et al., "A (Incorporated in the United Kingdom) phosphatidylinositol 3-kinase ", pp.642 647.
Gower Street, LONDON, WC1E 9BT, Unrted Kingdom AlDS(London), Vol.12, 1998, Del Real, G. et al., "Suppression of HIV-1...", pp.865 872.
(72) Inventor(s) Science, Vol.284, 1999, Daniel, R. et al., "A role for lan George Charles DNA-PK ", pp.644 647.
Weiming Xu Lizhi Liu (56) and (58) continued overleaf (74) continued overleaf (54) Abstract Title Inducible screen for drug discovery (57) A method for identifying a polynucleotide, the expression of which is modulated in the presence of nitric oxide (NO), which method comprises: (i) providing an mRNA or cDNA population from cells which contain a polynucleotide construct, which construct comprises: (a) a promoter operably linked to a coding sequence, wherein the promoter is responsive to ecdysone or an analog thereof and the coding sequence encodes a nitric oxide synthase (NOS) or a functional variant thereof: or (b) a promoter operably linked to one or more tetracycline operator site sequences and a coding sequence in that order, wherein the coding sequence encodes a nitric oxide synthase (NOS) or a functional variant thereof: (ii) providing an mRNA or cDNA population from cells as defined in step (i), said cells having been contacted with cedysone or an analog thereof: and (iii) comparing the populations of steps (i) and (ii), thereby to determine which polynucleotides show modulated expression in the presence of NO.
G) W CD n D
174) Agent and/or Address for Service (56) cant J A Kemp & Co. RN.A.S., Vol.93, 1996, No, et al., "Ecdysone-inducible 14 South Square, Gray's Inn, LONDON, WC1R 5JJ, expression ", pp.3346-3351.
United Kingdom Circulation, Vol.98, 1998, Iwashina, M. et al., "Transfection of inducible nitric oxide...", pp.1212-1218. Circulation, Vol.95, 1997, Kawaguchi, H. et al., "In viva transfection of...", pp.24412447.
Arterio. Thromb. Vasc. Biol., Vol., 17, 1997, Kullo, l. J. et al., "Expression and function of recombinant...", pp.2405-2412 Circ. Res., Vol. 80, 1997, Chen, A. F. Y. et al., "Expression and function of recombinant.. .", pp.327-335 (58) Field of Search by ISA
INT CL7 C12N, C12Q
Online: EPO-lnternal, WPI, PAJ, CHEM ABS, MEDUNE, EMBASE, BIOSIS
GB0204762A 1999-07-30 2000-07-28 Inducible screen for drug discovery Withdrawn GB2371547A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9918077.0A GB9918077D0 (en) 1999-07-30 1999-07-30 Inducible screen for drug discovery
GB0016171A GB0016171D0 (en) 2000-06-30 2000-06-30 Inducible screen for drug discovery
PCT/GB2000/002932 WO2001009375A2 (en) 1999-07-30 2000-07-28 Inducible screen for drug discovery

Publications (2)

Publication Number Publication Date
GB0204762D0 GB0204762D0 (en) 2002-04-17
GB2371547A true GB2371547A (en) 2002-07-31

Family

ID=26244580

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0204762A Withdrawn GB2371547A (en) 1999-07-30 2000-07-28 Inducible screen for drug discovery

Country Status (5)

Country Link
EP (1) EP1203098A2 (en)
AU (1) AU6454600A (en)
CA (1) CA2380323A1 (en)
GB (1) GB2371547A (en)
WO (1) WO2001009375A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004017950A2 (en) * 2002-08-22 2004-03-04 Piramed Limited Phosphadidylinositol 3,5-biphosphate inhibitors as anti-viral agents

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654168A (en) * 1994-07-01 1997-08-05 Basf Aktiengesellschaft Tetracycline-inducible transcriptional activator and tetracycline-regulated transcription units
US5759836A (en) * 1995-03-27 1998-06-02 Hospital For Joint Diseases Osteoarthritis-associated inducable isoform of nitric oxide synthetase
WO1998026066A1 (en) * 1996-12-09 1998-06-18 Ariad Gene Therapeutics, Inc. Expression of proteins for treating asthma via ligand mediated activation of their encoding genes
US5840521A (en) * 1991-02-26 1998-11-24 Imperial Chemical Industriles Plc Expression vector containing an inducible selection gene system
WO1999011620A1 (en) * 1997-08-28 1999-03-11 Pfizer Products Inc. Branched alkoxy-subsituted 2-aminopyridines as nos inhibitors
US5908756A (en) * 1996-08-30 1999-06-01 Johns Hopkins University Protein inhibitor of neuronal nitric oxide synthase
WO1999033971A1 (en) * 1997-12-31 1999-07-08 Medical College Of Georgia Research Institute, Inc. Oligomers that bind to ku protein
US5922927A (en) * 1993-06-14 1999-07-13 Basf Aktiengesellschaft Methods for producing tetracycline-regulated transgenic mice

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5840521A (en) * 1991-02-26 1998-11-24 Imperial Chemical Industriles Plc Expression vector containing an inducible selection gene system
US5922927A (en) * 1993-06-14 1999-07-13 Basf Aktiengesellschaft Methods for producing tetracycline-regulated transgenic mice
US5654168A (en) * 1994-07-01 1997-08-05 Basf Aktiengesellschaft Tetracycline-inducible transcriptional activator and tetracycline-regulated transcription units
US5759836A (en) * 1995-03-27 1998-06-02 Hospital For Joint Diseases Osteoarthritis-associated inducable isoform of nitric oxide synthetase
US5908756A (en) * 1996-08-30 1999-06-01 Johns Hopkins University Protein inhibitor of neuronal nitric oxide synthase
WO1998026066A1 (en) * 1996-12-09 1998-06-18 Ariad Gene Therapeutics, Inc. Expression of proteins for treating asthma via ligand mediated activation of their encoding genes
WO1999011620A1 (en) * 1997-08-28 1999-03-11 Pfizer Products Inc. Branched alkoxy-subsituted 2-aminopyridines as nos inhibitors
WO1999033971A1 (en) * 1997-12-31 1999-07-08 Medical College Of Georgia Research Institute, Inc. Oligomers that bind to ku protein

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
AIDS(London), Vol.12, 1998, Del Real, G. et al., "Suppression of HIV-1...", pp.865-872. *
Arterio. Thromb. Vasc. Biol., Vol., 17, 1997, Kullo, I. J. et al., "Expression and function of recombinant...", pp.2405-2412 *
Cancer Res., Vol.58, 1998, Christodoulopoulos, G. et al., "Potentiation of chlorambucil cytotoxicity...", pp.1789-1792. *
Circ. Res., Vol.80, 1997, Chen, A. F. Y. et al., "Expression and function of recombinant...", pp.327-335 *
Circulation, Vol.95, 1997, Kawaguchi, H. et al., "In vivo transfection of...", pp.2441-2447. *
Circulation, Vol.98, 1998, Iwashina, M. et al., "Transfection of inducible nitric oxide...", pp.1212-1218. *
Int. J. Cancer, Vol.78, 1998, Hosoi, Y. et al., "A phosphatidylinositol 3-kinase...", pp.642-647. *
P.N.A.S., Vol.93, 1996, No, et al., "Ecdysone-inducible expression...", pp.3346-3351. *
Science, Vol.284, 1999, Daniel, R. et al., "A role for DNA-PK...", pp.644-647. *

Also Published As

Publication number Publication date
GB0204762D0 (en) 2002-04-17
CA2380323A1 (en) 2001-02-08
AU6454600A (en) 2001-02-19
WO2001009375A3 (en) 2001-05-25
WO2001009375A2 (en) 2001-02-08
EP1203098A2 (en) 2002-05-08

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