WO2001009305A3 - Purine metabolism genes in plants - Google Patents

Purine metabolism genes in plants Download PDF

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Publication number
WO2001009305A3
WO2001009305A3 PCT/US2000/021009 US0021009W WO0109305A3 WO 2001009305 A3 WO2001009305 A3 WO 2001009305A3 US 0021009 W US0021009 W US 0021009W WO 0109305 A3 WO0109305 A3 WO 0109305A3
Authority
WO
WIPO (PCT)
Prior art keywords
deaminase
nucleic acid
adenosine deaminase
acid fragment
plants
Prior art date
Application number
PCT/US2000/021009
Other languages
French (fr)
Other versions
WO2001009305A2 (en
Inventor
Timothy Caspar
Saverio Carl Falco
Hajime Sakai
Zude Weng
Xu Hu
Original Assignee
Du Pont
Pioneer Hi Bred Int
Timothy Caspar
Saverio Carl Falco
Hajime Sakai
Zude Weng
Xu Hu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Pioneer Hi Bred Int, Timothy Caspar, Saverio Carl Falco, Hajime Sakai, Zude Weng, Xu Hu filed Critical Du Pont
Priority to EP00952383A priority Critical patent/EP1200568A2/en
Priority to JP2001514097A priority patent/JP2003506036A/en
Priority to CA002377064A priority patent/CA2377064A1/en
Priority to AU65092/00A priority patent/AU6509200A/en
Publication of WO2001009305A2 publication Critical patent/WO2001009305A2/en
Publication of WO2001009305A3 publication Critical patent/WO2001009305A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/78Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

This invention relates to an isolated nucleic acid fragment encoding an AMP deaminase or adenosine deaminase, a transformed host cell comprising the nucleic acid fragment, and a transgenic plant comprising the nucleic acid fragment. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of an AMP or adenosine deaminase, and a chimeric gene comprising the isolated fragment in sense or antisense orientation. This invention further relates to a method for altering expression level of AMP deaminase or adenosine deaminase in a transformed host cell, and a method for evaluating a compound that affects the activity of an AMP deaminase or an adenosine deaminase.
PCT/US2000/021009 1999-07-30 2000-07-28 Purine metabolism genes in plants WO2001009305A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00952383A EP1200568A2 (en) 1999-07-30 2000-07-28 Purine metabolism genes in plants
JP2001514097A JP2003506036A (en) 1999-07-30 2000-07-28 Purine metabolism genes in plants
CA002377064A CA2377064A1 (en) 1999-07-30 2000-07-28 Purine metabolism genes in plants
AU65092/00A AU6509200A (en) 1999-07-30 2000-07-28 Purine metabolism genes in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14647399P 1999-07-30 1999-07-30
US60/146,473 1999-07-30

Publications (2)

Publication Number Publication Date
WO2001009305A2 WO2001009305A2 (en) 2001-02-08
WO2001009305A3 true WO2001009305A3 (en) 2001-09-13

Family

ID=22517521

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/021009 WO2001009305A2 (en) 1999-07-30 2000-07-28 Purine metabolism genes in plants

Country Status (5)

Country Link
EP (1) EP1200568A2 (en)
JP (1) JP2003506036A (en)
AU (1) AU6509200A (en)
CA (1) CA2377064A1 (en)
WO (1) WO2001009305A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10035084A1 (en) * 2000-07-17 2002-02-07 Aventis Cropscience Gmbh Nucleic acid molecule encoding a plant AMP deaminase
CN1950501B (en) 2004-04-28 2012-06-13 天野酶株式会社 AMP deaminase originating in streptomyces and utilization thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DANCER ET AL: "Adenosine-5'-phosphate deaminase", PLANT PHYSIOLOGY,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD,US, vol. 114, 1997, pages 119 - 129, XP002110870, ISSN: 0032-0889 *
DATABASE EMBL 12 June 1999 (1999-06-12), BLEWITT ET AL.: "ESTs from developing cotton fiber", XP002150378 *
DATABASE NCBI 14 May 1999 (1999-05-14), ROUNSLEY ET AL.: "AMP deaminase homolog F16M14.21 - Arabidopsis thaliana", XP002150377 *

Also Published As

Publication number Publication date
WO2001009305A2 (en) 2001-02-08
JP2003506036A (en) 2003-02-18
EP1200568A2 (en) 2002-05-02
AU6509200A (en) 2001-02-19
CA2377064A1 (en) 2001-02-08

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