WO2001007911A3 - Nouveau procede d'isolement perfectionne d'une population cellulaire cible - Google Patents

Nouveau procede d'isolement perfectionne d'une population cellulaire cible Download PDF

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Publication number
WO2001007911A3
WO2001007911A3 PCT/DK2000/000408 DK0000408W WO0107911A3 WO 2001007911 A3 WO2001007911 A3 WO 2001007911A3 DK 0000408 W DK0000408 W DK 0000408W WO 0107911 A3 WO0107911 A3 WO 0107911A3
Authority
WO
WIPO (PCT)
Prior art keywords
target cell
cell population
cells
sample
novel method
Prior art date
Application number
PCT/DK2000/000408
Other languages
English (en)
Other versions
WO2001007911A2 (fr
Inventor
Britta Christensen
Steen Koelvraa
John Philip
Original Assignee
Britta Christensen
Steen Koelvraa
John Philip
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Britta Christensen, Steen Koelvraa, John Philip filed Critical Britta Christensen
Priority to AU59671/00A priority Critical patent/AU5967100A/en
Publication of WO2001007911A2 publication Critical patent/WO2001007911A2/fr
Publication of WO2001007911A3 publication Critical patent/WO2001007911A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/505—Erythropoietin [EPO]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70582—CD71

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé d'isolement d'au moins une population cellulaire cible, à partir d'un échantillon liquide de cellules. Ce procédé consiste à établir un écoulement de l'échantillon sur une surface revêtue d'anticorps spécifiques de la population cellulaire cible, et à permettre à l'échantillon de cellules et à la surface d'être en contact physique, ce qui contribue à la liaison des cellules provenant d'au moins une population cellulaire cible mammifère avec les anticorps présents sur la surface. Le procédé consiste ensuite à enlever de la surface l'échantillon liquide comprenant les cellules non liées, et à identifier les cellules cibles restant sur la surface. L'invention concerne en outre l'utilisation d'un tel procédé, ainsi qu'un système d'isolement de populations cellulaires cibles à partir d'un échantillon liquide de cellules.
PCT/DK2000/000408 1999-07-21 2000-07-18 Nouveau procede d'isolement perfectionne d'une population cellulaire cible WO2001007911A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU59671/00A AU5967100A (en) 1999-07-21 2000-07-18 A novel method for the improved isolation of a target cell population

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199901052 1999-07-21
DKPA199901052 1999-07-21

Publications (2)

Publication Number Publication Date
WO2001007911A2 WO2001007911A2 (fr) 2001-02-01
WO2001007911A3 true WO2001007911A3 (fr) 2001-11-08

Family

ID=8100444

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2000/000408 WO2001007911A2 (fr) 1999-07-21 2000-07-18 Nouveau procede d'isolement perfectionne d'une population cellulaire cible

Country Status (2)

Country Link
AU (1) AU5967100A (fr)
WO (1) WO2001007911A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3082861A1 (fr) * 2017-12-01 2019-06-06 General Electric Company Procedes d'enrichissement et d'isolement de cellules

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990006509A1 (fr) * 1988-12-06 1990-06-14 Flinders Technologies Pty. Ltd. Procede servant a isoler des cellules f×tales du sang maternel en vue d'un diagnostic prenatal
WO1993023754A1 (fr) * 1992-05-16 1993-11-25 Dorothee Ahlert Procede de diagnostic prenatal d'anomalies genetiques
US5437987A (en) * 1992-09-25 1995-08-01 The Board Of Trustees Of The Leland Stanford Junior University Triple gradient process with antibody panning to recover nucleated fetal cells from maternal blood
EP0701130A2 (fr) * 1989-06-29 1996-03-13 Applied Immunesciences, Inc. Appareil et procédé de saisie et de récupération de cellules

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990006509A1 (fr) * 1988-12-06 1990-06-14 Flinders Technologies Pty. Ltd. Procede servant a isoler des cellules f×tales du sang maternel en vue d'un diagnostic prenatal
EP0701130A2 (fr) * 1989-06-29 1996-03-13 Applied Immunesciences, Inc. Appareil et procédé de saisie et de récupération de cellules
WO1993023754A1 (fr) * 1992-05-16 1993-11-25 Dorothee Ahlert Procede de diagnostic prenatal d'anomalies genetiques
US5437987A (en) * 1992-09-25 1995-08-01 The Board Of Trustees Of The Leland Stanford Junior University Triple gradient process with antibody panning to recover nucleated fetal cells from maternal blood

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WYSOCKI L J ET AL: "Panning" for lymphocytes: a method for cell selection", PROC. NATL. ACAD. SCI. USA, vol. 75, no. 6, June 1978 (1978-06-01), pages 2844 - 2848, XP002901409 *

Also Published As

Publication number Publication date
WO2001007911A2 (fr) 2001-02-01
AU5967100A (en) 2001-02-13

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