WO2001007639A3 - Selectable marker gene - Google Patents

Selectable marker gene Download PDF

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Publication number
WO2001007639A3
WO2001007639A3 PCT/GB2000/002673 GB0002673W WO0107639A3 WO 2001007639 A3 WO2001007639 A3 WO 2001007639A3 GB 0002673 W GB0002673 W GB 0002673W WO 0107639 A3 WO0107639 A3 WO 0107639A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
selectable marker
marker gene
agent
glyoxalase
Prior art date
Application number
PCT/GB2000/002673
Other languages
French (fr)
Other versions
WO2001007639A2 (en
Inventor
Mark Skipsey
Robert Edwards
Original Assignee
Syngenta Ltd
Mark Skipsey
Robert Edwards
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Ltd, Mark Skipsey, Robert Edwards filed Critical Syngenta Ltd
Priority to AU61672/00A priority Critical patent/AU6167200A/en
Publication of WO2001007639A2 publication Critical patent/WO2001007639A2/en
Publication of WO2001007639A3 publication Critical patent/WO2001007639A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A method of selecting transformed cells comprising stably transforming the genome of the cell with a nucleic acid encoding a glyoxalase I protein and subjecting the cell to a selective agent comprising a normally toxic agent which is toxic to untransformed cells but is capable of being degraded by the glyoxalase thereby eliminating the toxicity of the agent to the transformed cell.
PCT/GB2000/002673 1999-07-27 2000-07-12 Selectable marker gene WO2001007639A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU61672/00A AU6167200A (en) 1999-07-27 2000-07-12 Selectable marker gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9917643.0A GB9917643D0 (en) 1999-07-27 1999-07-27 Selectable marker gene
GB9917643.0 1999-07-27

Publications (2)

Publication Number Publication Date
WO2001007639A2 WO2001007639A2 (en) 2001-02-01
WO2001007639A3 true WO2001007639A3 (en) 2001-08-09

Family

ID=10858038

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/002673 WO2001007639A2 (en) 1999-07-27 2000-07-12 Selectable marker gene

Country Status (3)

Country Link
AU (1) AU6167200A (en)
GB (1) GB9917643D0 (en)
WO (1) WO2001007639A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2562259B1 (en) 2004-12-21 2016-07-06 Monsanto Technology LLC Transgenic plants with enhanced agronomic traits
CN103305635B (en) * 2013-06-18 2015-02-25 福建农林大学 Method for molecularly detecting sorghum mosaic virus infecting sugarcane

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0568571A (en) * 1991-08-13 1993-03-23 Sagami Chem Res Center Dna capable of coding human-derived glyoxalase i

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0568571A (en) * 1991-08-13 1993-03-23 Sagami Chem Res Center Dna capable of coding human-derived glyoxalase i

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CLUGSTON S L ET AL: "Isolation and sequencing of a gene coding for glyoxalase I activity from Salmonella typhimurium and comparison with other glyoxalase I sequences", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES,GB,ELSEVIER SCIENCE PUBLISHERS, BARKING, vol. 186, no. 1, 20 February 1997 (1997-02-20), pages 103 - 111, XP004054887, ISSN: 0378-1119 *
DATABASE EMBL SEQUENCE LIBRARY 7 January 1999 (1999-01-07), SKIPSEY, M., ET AL.: "cloning and characterization of soybean glyoxylase I - unpublished", XP002154136 *
DATABASE WPI Section Ch Week 199316, Derwent World Patents Index; Class B04, AN 1993-130643, XP002154137 *
ESPARTERO JOAQUIN ET AL: "Molecular characterization of glyoxalase-I from a higher plant: Upregulation by stress.", PLANT MOLECULAR BIOLOGY, vol. 29, no. 6, 1995, pages 1223 - 1233, XP002154134, ISSN: 0167-4412 *
SKIPSEY MARK ET AL: "Cloning and characterization of glyoxalase I from soybean.", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS., vol. 374, no. 2, 15 February 2000 (2000-02-15), pages 261 - 268, XP002154135, ISSN: 0003-9861 *
VEENA ET AL: "Glyoxalase I from Brassica juncea: Molecular cloning, regulation and its over-expression confer tolerance in transgenic tobacco under stress.", PLANT JOURNAL, vol. 17, no. 4, February 1999 (1999-02-01), pages 385 - 395, XP002154133, ISSN: 0960-7412 *

Also Published As

Publication number Publication date
GB9917643D0 (en) 1999-09-29
AU6167200A (en) 2001-02-13
WO2001007639A2 (en) 2001-02-01

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