AU2001281911A1 - Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current - Google Patents
Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical currentInfo
- Publication number
- AU2001281911A1 AU2001281911A1 AU2001281911A AU2001281911A AU2001281911A1 AU 2001281911 A1 AU2001281911 A1 AU 2001281911A1 AU 2001281911 A AU2001281911 A AU 2001281911A AU 2001281911 A AU2001281911 A AU 2001281911A AU 2001281911 A1 AU2001281911 A1 AU 2001281911A1
- Authority
- AU
- Australia
- Prior art keywords
- nucleus
- nucleic acids
- biologically active
- electrical current
- eukaryotic cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004940 Nucleus Anatomy 0.000 title 1
- 210000003527 eukaryotic cell Anatomy 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10031179A DE10031179A1 (en) | 2000-06-27 | 2000-06-27 | Introducing active molecules into the nucleus of eukaryotic cells, useful for transfection and gene therapy, by applying controlled electrical pulse |
DE10031179.2 | 2000-06-27 | ||
PCT/EP2001/007348 WO2002000871A2 (en) | 2000-06-27 | 2001-06-27 | Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current |
Publications (2)
Publication Number | Publication Date |
---|---|
AU2001281911A1 true AU2001281911A1 (en) | 2002-03-28 |
AU2001281911B2 AU2001281911B2 (en) | 2005-03-10 |
Family
ID=7646911
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU8191101A Pending AU8191101A (en) | 2000-06-27 | 2001-06-27 | Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current |
AU2001281911A Expired AU2001281911B2 (en) | 2000-06-27 | 2001-06-27 | Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU8191101A Pending AU8191101A (en) | 2000-06-27 | 2001-06-27 | Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current |
Country Status (11)
Country | Link |
---|---|
US (1) | US20040014220A1 (en) |
EP (1) | EP1297119B9 (en) |
JP (1) | JP4794111B2 (en) |
CN (1) | CN100381176C (en) |
AT (1) | ATE282090T1 (en) |
AU (2) | AU8191101A (en) |
CA (1) | CA2414542C (en) |
DE (2) | DE10031179A1 (en) |
ES (1) | ES2232653T3 (en) |
IL (1) | IL153660A0 (en) |
WO (1) | WO2002000871A2 (en) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE50201240D1 (en) | 2001-04-23 | 2004-11-11 | Amaxa Gmbh | BUFFER SOLUTION FOR ELECTROPORATION AND METHOD COMPREHENSIVE USE |
WO2004029620A2 (en) * | 2002-09-26 | 2004-04-08 | Novartis Ag | Screening method involving transfected primary t cells |
US7598071B2 (en) * | 2004-07-09 | 2009-10-06 | The United States Of America As Represented By The Department Of Health And Human Services | Infectious clone of human parvovirus B19 and methods |
EP1741778B1 (en) * | 2005-07-07 | 2007-12-26 | Amaxa AG | Procedure for the treatment of small volumes with electric current |
US8129187B2 (en) | 2005-12-13 | 2012-03-06 | Kyoto University | Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2 |
EP2208786B1 (en) | 2005-12-13 | 2018-08-01 | Kyoto University | Nuclear reprogramming factor |
US8278104B2 (en) | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
DE102007005909A1 (en) | 2007-02-01 | 2008-08-14 | Amaxa Ag | Method for controlling quality of container filled with liquid, involves carrying out distinction between defective and error free container by determination of capacity of condenser |
US9213999B2 (en) | 2007-06-15 | 2015-12-15 | Kyoto University | Providing iPSCs to a customer |
JP2008307007A (en) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth |
CA2695522C (en) | 2008-05-02 | 2019-01-15 | Kyoto University | Method of nuclear reprogramming |
EP2208778A1 (en) | 2009-01-20 | 2010-07-21 | Lonza Cologne AG | Method and device for electric processing of reaction areas |
WO2012098260A1 (en) | 2011-01-21 | 2012-07-26 | Axiogenesis Ag | A non-viral system for the generation of induced pluripotent stem (ips) cells |
US10093706B2 (en) | 2017-01-30 | 2018-10-09 | Indiana University Research And Technology Corporation | Dominant positive hnRNP-E1 polypeptide compositions and methods |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4849355A (en) * | 1985-01-08 | 1989-07-18 | Wong Tai Kin | Method of transferring genes into cells |
US4750100A (en) * | 1986-06-06 | 1988-06-07 | Bio-Rad Laboratories | Transfection high voltage controller |
US5098843A (en) * | 1987-06-04 | 1992-03-24 | Calvin Noel M | Apparatus for the high efficiency transformation of living cells |
US5128257A (en) * | 1987-08-31 | 1992-07-07 | Baer Bradford W | Electroporation apparatus and process |
CA1327174C (en) * | 1988-10-05 | 1994-02-22 | Youssef Mouneimne | Electro-insertion of proteins into animal cell membranes |
BE1004328A3 (en) * | 1990-05-16 | 1992-11-03 | Scient Equipment Design & Dev | METHOD AND DEVICE FOR LIVING CELLS permeabilization. |
CA2019758C (en) * | 1990-06-25 | 2001-09-04 | Kevin L. Firth | Improved electroporation device and method |
AU8856091A (en) * | 1990-09-27 | 1992-04-28 | United States of America, as represented by the Secretary, U.S. Department of Commerce, The | Method of electroporation using bipolar oscillating electric fields |
US5273525A (en) * | 1992-08-13 | 1993-12-28 | Btx Inc. | Injection and electroporation apparatus for drug and gene delivery |
US5627023A (en) * | 1993-03-29 | 1997-05-06 | Duke University | Suppressor of HIV replication and transcription |
US6103084A (en) * | 1995-06-06 | 2000-08-15 | Eppendorf Netheler-Hinz Gmbh | Apparatus for electroporation |
US5869326A (en) * | 1996-09-09 | 1999-02-09 | Genetronics, Inc. | Electroporation employing user-configured pulsing scheme |
EP0866123B1 (en) * | 1997-03-21 | 2005-03-16 | Eppendorf Ag | Method and circuit device for electropermeation or electroporation of living cells |
SE9704076D0 (en) * | 1997-11-06 | 1997-11-06 | Holdingbolaget Vid Goeteborgs | Method for permeabilization of cell structures and use thereof |
WO1999036563A1 (en) * | 1998-01-14 | 1999-07-22 | Emed Corporation | Electrically mediated cellular expression |
US6632672B2 (en) * | 1998-08-19 | 2003-10-14 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for genomic modification |
US6150148A (en) * | 1998-10-21 | 2000-11-21 | Genetronics, Inc. | Electroporation apparatus for control of temperature during the process |
EP2105124A1 (en) * | 2008-03-26 | 2009-09-30 | Bayer MaterialScience AG | Sunscreen compositions |
-
2000
- 2000-06-27 DE DE10031179A patent/DE10031179A1/en not_active Ceased
-
2001
- 2001-06-27 AU AU8191101A patent/AU8191101A/en active Pending
- 2001-06-27 CA CA2414542A patent/CA2414542C/en not_active Expired - Lifetime
- 2001-06-27 ES ES01960408T patent/ES2232653T3/en not_active Expired - Lifetime
- 2001-06-27 IL IL15366001A patent/IL153660A0/en unknown
- 2001-06-27 EP EP01960408A patent/EP1297119B9/en not_active Expired - Lifetime
- 2001-06-27 CN CNB018119557A patent/CN100381176C/en not_active Expired - Lifetime
- 2001-06-27 AU AU2001281911A patent/AU2001281911B2/en not_active Expired
- 2001-06-27 JP JP2002506187A patent/JP4794111B2/en not_active Expired - Lifetime
- 2001-06-27 WO PCT/EP2001/007348 patent/WO2002000871A2/en active IP Right Grant
- 2001-06-27 US US10/312,244 patent/US20040014220A1/en not_active Abandoned
- 2001-06-27 AT AT01960408T patent/ATE282090T1/en active
- 2001-06-27 DE DE50104496T patent/DE50104496D1/en not_active Expired - Lifetime
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