AU2001281911A1 - Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current - Google Patents

Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current

Info

Publication number
AU2001281911A1
AU2001281911A1 AU2001281911A AU2001281911A AU2001281911A1 AU 2001281911 A1 AU2001281911 A1 AU 2001281911A1 AU 2001281911 A AU2001281911 A AU 2001281911A AU 2001281911 A AU2001281911 A AU 2001281911A AU 2001281911 A1 AU2001281911 A1 AU 2001281911A1
Authority
AU
Australia
Prior art keywords
nucleus
nucleic acids
biologically active
electrical current
eukaryotic cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU2001281911A
Other versions
AU2001281911B2 (en
Inventor
Ludger Altrogge
Rainer Christine
Marion Gremse
Dietmar Lenz
Sabine Poppenborg
Gudula Riemen
Kirsten Rothmann
Gregor Siebenkotten
Corinna Thiel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza Cologne GmbH
Original Assignee
Lonza Cologne GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10031179A external-priority patent/DE10031179A1/en
Application filed by Lonza Cologne GmbH filed Critical Lonza Cologne GmbH
Publication of AU2001281911A1 publication Critical patent/AU2001281911A1/en
Application granted granted Critical
Publication of AU2001281911B2 publication Critical patent/AU2001281911B2/en
Assigned to AMAXA AG reassignment AMAXA AG Request for Assignment Assignors: AMAXA GMBH
Assigned to LONZA COLOGNE AG reassignment LONZA COLOGNE AG Request to Amend Deed and Register Assignors: AMAXA AG
Assigned to LONZA COLOGNE AG reassignment LONZA COLOGNE AG Request to Amend Deed and Register Assignors: AMAXA AG
Assigned to LONZA COLOGNE GMBH reassignment LONZA COLOGNE GMBH Request to Amend Deed and Register Assignors: LONZA COLOGNE AG
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

AU2001281911A 2000-06-27 2001-06-27 Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current Expired AU2001281911B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10031179A DE10031179A1 (en) 2000-06-27 2000-06-27 Introducing active molecules into the nucleus of eukaryotic cells, useful for transfection and gene therapy, by applying controlled electrical pulse
DE10031179.2 2000-06-27
PCT/EP2001/007348 WO2002000871A2 (en) 2000-06-27 2001-06-27 Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current

Publications (2)

Publication Number Publication Date
AU2001281911A1 true AU2001281911A1 (en) 2002-03-28
AU2001281911B2 AU2001281911B2 (en) 2005-03-10

Family

ID=7646911

Family Applications (2)

Application Number Title Priority Date Filing Date
AU8191101A Pending AU8191101A (en) 2000-06-27 2001-06-27 Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current
AU2001281911A Expired AU2001281911B2 (en) 2000-06-27 2001-06-27 Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AU8191101A Pending AU8191101A (en) 2000-06-27 2001-06-27 Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current

Country Status (11)

Country Link
US (1) US20040014220A1 (en)
EP (1) EP1297119B9 (en)
JP (1) JP4794111B2 (en)
CN (1) CN100381176C (en)
AT (1) ATE282090T1 (en)
AU (2) AU8191101A (en)
CA (1) CA2414542C (en)
DE (2) DE10031179A1 (en)
ES (1) ES2232653T3 (en)
IL (1) IL153660A0 (en)
WO (1) WO2002000871A2 (en)

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DE50201240D1 (en) 2001-04-23 2004-11-11 Amaxa Gmbh BUFFER SOLUTION FOR ELECTROPORATION AND METHOD COMPREHENSIVE USE
WO2004029620A2 (en) * 2002-09-26 2004-04-08 Novartis Ag Screening method involving transfected primary t cells
US7598071B2 (en) * 2004-07-09 2009-10-06 The United States Of America As Represented By The Department Of Health And Human Services Infectious clone of human parvovirus B19 and methods
EP1741778B1 (en) * 2005-07-07 2007-12-26 Amaxa AG Procedure for the treatment of small volumes with electric current
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
EP2208786B1 (en) 2005-12-13 2018-08-01 Kyoto University Nuclear reprogramming factor
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
DE102007005909A1 (en) 2007-02-01 2008-08-14 Amaxa Ag Method for controlling quality of container filled with liquid, involves carrying out distinction between defective and error free container by determination of capacity of condenser
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
JP2008307007A (en) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
CA2695522C (en) 2008-05-02 2019-01-15 Kyoto University Method of nuclear reprogramming
EP2208778A1 (en) 2009-01-20 2010-07-21 Lonza Cologne AG Method and device for electric processing of reaction areas
WO2012098260A1 (en) 2011-01-21 2012-07-26 Axiogenesis Ag A non-viral system for the generation of induced pluripotent stem (ips) cells
US10093706B2 (en) 2017-01-30 2018-10-09 Indiana University Research And Technology Corporation Dominant positive hnRNP-E1 polypeptide compositions and methods

Family Cites Families (18)

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Publication number Priority date Publication date Assignee Title
US4849355A (en) * 1985-01-08 1989-07-18 Wong Tai Kin Method of transferring genes into cells
US4750100A (en) * 1986-06-06 1988-06-07 Bio-Rad Laboratories Transfection high voltage controller
US5098843A (en) * 1987-06-04 1992-03-24 Calvin Noel M Apparatus for the high efficiency transformation of living cells
US5128257A (en) * 1987-08-31 1992-07-07 Baer Bradford W Electroporation apparatus and process
CA1327174C (en) * 1988-10-05 1994-02-22 Youssef Mouneimne Electro-insertion of proteins into animal cell membranes
BE1004328A3 (en) * 1990-05-16 1992-11-03 Scient Equipment Design & Dev METHOD AND DEVICE FOR LIVING CELLS permeabilization.
CA2019758C (en) * 1990-06-25 2001-09-04 Kevin L. Firth Improved electroporation device and method
AU8856091A (en) * 1990-09-27 1992-04-28 United States of America, as represented by the Secretary, U.S. Department of Commerce, The Method of electroporation using bipolar oscillating electric fields
US5273525A (en) * 1992-08-13 1993-12-28 Btx Inc. Injection and electroporation apparatus for drug and gene delivery
US5627023A (en) * 1993-03-29 1997-05-06 Duke University Suppressor of HIV replication and transcription
US6103084A (en) * 1995-06-06 2000-08-15 Eppendorf Netheler-Hinz Gmbh Apparatus for electroporation
US5869326A (en) * 1996-09-09 1999-02-09 Genetronics, Inc. Electroporation employing user-configured pulsing scheme
EP0866123B1 (en) * 1997-03-21 2005-03-16 Eppendorf Ag Method and circuit device for electropermeation or electroporation of living cells
SE9704076D0 (en) * 1997-11-06 1997-11-06 Holdingbolaget Vid Goeteborgs Method for permeabilization of cell structures and use thereof
WO1999036563A1 (en) * 1998-01-14 1999-07-22 Emed Corporation Electrically mediated cellular expression
US6632672B2 (en) * 1998-08-19 2003-10-14 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for genomic modification
US6150148A (en) * 1998-10-21 2000-11-21 Genetronics, Inc. Electroporation apparatus for control of temperature during the process
EP2105124A1 (en) * 2008-03-26 2009-09-30 Bayer MaterialScience AG Sunscreen compositions

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