WO2001007565A3 - Monoterpene synthases from grand fir (abies grandis) - Google Patents

Monoterpene synthases from grand fir (abies grandis) Download PDF

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Publication number
WO2001007565A3
WO2001007565A3 PCT/US2000/020264 US0020264W WO0107565A3 WO 2001007565 A3 WO2001007565 A3 WO 2001007565A3 US 0020264 W US0020264 W US 0020264W WO 0107565 A3 WO0107565 A3 WO 0107565A3
Authority
WO
WIPO (PCT)
Prior art keywords
monoterpene
monoterpene synthases
recombinant
synthases
abies grandis
Prior art date
Application number
PCT/US2000/020264
Other languages
French (fr)
Other versions
WO2001007565A2 (en
Inventor
Christopher L Steele
Joerg Bohlmann
Rodney B Croteu
Michael A Phillips
Original Assignee
Univ Washington
Christopher L Steele
Joerg Bohlmann
Rodney B Croteu
Michael A Phillips
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US1998/014528 external-priority patent/WO1999002030A1/en
Application filed by Univ Washington, Christopher L Steele, Joerg Bohlmann, Rodney B Croteu, Michael A Phillips filed Critical Univ Washington
Priority to EP00948965A priority Critical patent/EP1220899A4/en
Priority to CA002380221A priority patent/CA2380221A1/en
Priority to AU62382/00A priority patent/AU6238200A/en
Publication of WO2001007565A2 publication Critical patent/WO2001007565A2/en
Publication of WO2001007565A3 publication Critical patent/WO2001007565A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Abstract

cDNAs encoding gymnosperm monoterpene synthases have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding a monoterpene synthase of the invention. Thus, systems and methods are provided for the recombinant expression of recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts.
PCT/US2000/020264 1997-07-11 2000-07-24 Monoterpene synthases from grand fir (abies grandis) WO2001007565A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00948965A EP1220899A4 (en) 1999-07-26 2000-07-24 Monoterpene synthases from grand fir (abies grandis)
CA002380221A CA2380221A1 (en) 1999-07-26 2000-07-24 Monoterpene synthases from grand fir (abies grandis)
AU62382/00A AU6238200A (en) 1999-07-26 2000-07-24 Monoterpene synthases from grand fir (abies grandis)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US5224997P 1997-07-11 1997-07-11
PCT/US1998/014528 WO1999002030A1 (en) 1997-07-11 1998-07-10 Monoterpene synthases from grand fir (abies grandis)
US09/360,545 US6429014B1 (en) 1997-07-11 1999-07-26 Monoterpene synthases from grand fir (Abies grandis)
US09/360,545 1999-07-26

Publications (2)

Publication Number Publication Date
WO2001007565A2 WO2001007565A2 (en) 2001-02-01
WO2001007565A3 true WO2001007565A3 (en) 2002-05-10

Family

ID=26730382

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/020264 WO2001007565A2 (en) 1997-07-11 2000-07-24 Monoterpene synthases from grand fir (abies grandis)

Country Status (2)

Country Link
US (2) US6429014B1 (en)
WO (1) WO2001007565A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9919409D0 (en) * 1999-08-18 1999-10-20 Univ Oxford Brookes Baculovirus expression system
US7704716B2 (en) * 2003-12-10 2010-04-27 The Regents Of The University Of Michigan Geraniol synthase, methods of production and uses thereof
US20090061493A1 (en) 2007-06-01 2009-03-05 Solazyme, Inc. Lipid Pathway Modification in Oil-Bearing Microorganisms
BRPI0921539A2 (en) 2008-11-28 2020-08-04 Solazyme, Inc. cell, triglyceride oil composition, oil blend, method of preparing a fuel by processing the oil, and fuel
SG185780A1 (en) 2010-05-28 2013-01-30 Solazyme Inc Tailored oils produced from recombinant heterotrophic microorganisms
EP3643774A1 (en) 2011-02-02 2020-04-29 Corbion Biotech, Inc. Tailored oils produced from recombinant oleaginous microorganisms
WO2016210154A1 (en) * 2015-06-26 2016-12-29 The Regents Of The University Of California Fusion constructs as protein over-expression vectors
CN112779242B (en) * 2019-11-07 2023-03-28 四川弘合生物科技有限公司 Plum tree monoterpene synthase CbTPS1 and related biological material and application thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4948811A (en) 1988-01-26 1990-08-14 The Procter & Gamble Company Salad/cooking oil balanced for health benefits
CA2118071C (en) 1993-10-28 2004-09-14 Rodney B. Croteau Dna encoding limonene synthase from mentha spicata

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LEWINSOHN ET AL.: "Wound-inducible pinene cyclase from grand fir: purification, characterization and renaturation after SDS-PAGE", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 293, no. 1, 14 February 1992 (1992-02-14), pages 167 - 173, XP002944497 *
WISE ET AL.: "Monoterpene synthases from common sage (Salvia Officinalis)", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 24, 12 June 1998 (1998-06-12), pages 14891 - 14899, XP002944496 *

Also Published As

Publication number Publication date
WO2001007565A2 (en) 2001-02-01
US20030175861A1 (en) 2003-09-18
US6429014B1 (en) 2002-08-06

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