WO2000052179A3 - Dna binding compound-mediated molecular switch system - Google Patents

Dna binding compound-mediated molecular switch system Download PDF

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Publication number
WO2000052179A3
WO2000052179A3 PCT/US2000/005728 US0005728W WO0052179A3 WO 2000052179 A3 WO2000052179 A3 WO 2000052179A3 US 0005728 W US0005728 W US 0005728W WO 0052179 A3 WO0052179 A3 WO 0052179A3
Authority
WO
WIPO (PCT)
Prior art keywords
switch system
molecular switch
promoter
dna binding
binding compound
Prior art date
Application number
PCT/US2000/005728
Other languages
French (fr)
Other versions
WO2000052179A2 (en
Inventor
Moon Young Lim
Cynthia A Edwards
Kirk E Fry
Thomas W Bruice
Douglas B Starr
Megan E Laurance
Yan Kwok
Albert W Tam
Original Assignee
Genelabs Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genelabs Tech Inc filed Critical Genelabs Tech Inc
Priority to EP00913742A priority Critical patent/EP1165808A2/en
Priority to CA002367037A priority patent/CA2367037A1/en
Priority to AU35130/00A priority patent/AU3513000A/en
Publication of WO2000052179A2 publication Critical patent/WO2000052179A2/en
Publication of WO2000052179A3 publication Critical patent/WO2000052179A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4738—Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/40—Systems of functionally co-operating vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/55—Vector systems having a special element relevant for transcription from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/75—Vector systems having a special element relevant for transcription from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides molecular switch system methods and compositions for use in regulatable gene expression. The system includes a nucleic acid construct which has a DNA response sequence for a transcriptional regulatory protein operably linked to a promoter, a compound binding sequence in the vicinity of the DNA response sequence, a transgene under the control of the promoter; and a DNA binding compound. In some cases, the molecular switch system further includes a nucleic acid sequence encoding a transcriptional regulatory protein operably linked to a second promoter. The invention further provides a method for screening compounds for the ability to function in the molecular switch system and thereby regulate gene expression.
PCT/US2000/005728 1999-03-03 2000-03-03 Dna binding compound-mediated molecular switch system WO2000052179A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00913742A EP1165808A2 (en) 1999-03-03 2000-03-03 Dna binding compound-mediated molecular switch system
CA002367037A CA2367037A1 (en) 1999-03-03 2000-03-03 Dna binding compound-mediated molecular switch system
AU35130/00A AU3513000A (en) 1999-03-03 2000-03-03 Dna binding compound-mediated molecular switch system

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US12251399P 1999-03-03 1999-03-03
US60/122,513 1999-03-03
US15460599P 1999-09-17 1999-09-17
US60/154,605 1999-09-17

Publications (2)

Publication Number Publication Date
WO2000052179A2 WO2000052179A2 (en) 2000-09-08
WO2000052179A3 true WO2000052179A3 (en) 2000-12-21

Family

ID=26820607

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/005728 WO2000052179A2 (en) 1999-03-03 2000-03-03 Dna binding compound-mediated molecular switch system

Country Status (4)

Country Link
EP (1) EP1165808A2 (en)
AU (1) AU3513000A (en)
CA (1) CA2367037A1 (en)
WO (1) WO2000052179A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001053479A2 (en) * 2000-01-24 2001-07-26 Sangamo Biosciences, Inc. Molecular switches ii
US6838556B2 (en) 2000-06-06 2005-01-04 Genelabs Technologies, Inc. Promoters for regulated gene expression
WO2002048195A2 (en) * 2000-10-23 2002-06-20 Engeneos, Inc. Engineered stimulus-responsive switches
ES2299284B1 (en) * 2004-11-04 2009-04-16 Universidad Pablo De Olavide CONTROL OF GENE EXPRESSION THROUGH THE USE OF A TRANSCRIPTION ATTENATOR.

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993023431A1 (en) * 1992-05-14 1993-11-25 Baylor College Of Medicine Mutated steroid hormone receptors, methods for their use and molecular switch for gene therapy
WO1994029442A2 (en) * 1993-06-14 1994-12-22 Basf Aktiengesellschaft Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
WO1996037609A1 (en) * 1995-05-26 1996-11-28 Zeneca Limited A gene switch comprising an ecdysone receptor
WO1996041865A1 (en) * 1995-06-07 1996-12-27 Ariad Gene Therapeutics, Inc. Rapamcycin-based regulation of biological events
WO1997010337A1 (en) * 1995-09-15 1997-03-20 Baylor College Of Medicine Steroid receptor coactivator compositions and methods of use
WO1999025856A1 (en) * 1997-11-14 1999-05-27 Heddle John A System and method for regulation of gene expression
WO2000009704A1 (en) * 1998-08-13 2000-02-24 Syngenta Limited Gene switch

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993023431A1 (en) * 1992-05-14 1993-11-25 Baylor College Of Medicine Mutated steroid hormone receptors, methods for their use and molecular switch for gene therapy
WO1994029442A2 (en) * 1993-06-14 1994-12-22 Basf Aktiengesellschaft Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
WO1996037609A1 (en) * 1995-05-26 1996-11-28 Zeneca Limited A gene switch comprising an ecdysone receptor
WO1996041865A1 (en) * 1995-06-07 1996-12-27 Ariad Gene Therapeutics, Inc. Rapamcycin-based regulation of biological events
WO1997010337A1 (en) * 1995-09-15 1997-03-20 Baylor College Of Medicine Steroid receptor coactivator compositions and methods of use
WO1999025856A1 (en) * 1997-11-14 1999-05-27 Heddle John A System and method for regulation of gene expression
WO2000009704A1 (en) * 1998-08-13 2000-02-24 Syngenta Limited Gene switch

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHEN^1 G ET AL: "Groucho/TLE family proteins and transcriptional repression", GENE,NL,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, vol. 249, no. 1-2, May 2000 (2000-05-01), pages 1 - 16, XP000946056, ISSN: 0378-1119 *
HIDALGO A: "The roles of engrailed", TRENDS IN GENETICS,NL,ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, vol. 12, no. 1, 1996, pages 1 - 4, XP004037189, ISSN: 0168-9525 *
MARGOLIN JUDITH F ET AL: "Krueppel-associated boxes are potent transcriptional repression domains", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 91, no. 10, May 1994 (1994-05-01), pages 4509 - 4513, XP002136979, ISSN: 0027-8424 *
RUSSO M W ET AL: "IDENTIFICATION OF NAB1 A REPRESSOR OF NGFI-A- AND KROX20- MEDIATED TRANSCRIPTION", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 92, 1 July 1995 (1995-07-01), pages 6873 - 6877, XP002034500, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
AU3513000A (en) 2000-09-21
CA2367037A1 (en) 2000-09-08
WO2000052179A2 (en) 2000-09-08
EP1165808A2 (en) 2002-01-02

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