WO2001007159A3 - Integration of biochemical protocols in a continuous flow microfluidic device - Google Patents

Integration of biochemical protocols in a continuous flow microfluidic device Download PDF

Info

Publication number
WO2001007159A3
WO2001007159A3 PCT/IB2000/001137 IB0001137W WO0107159A3 WO 2001007159 A3 WO2001007159 A3 WO 2001007159A3 IB 0001137 W IB0001137 W IB 0001137W WO 0107159 A3 WO0107159 A3 WO 0107159A3
Authority
WO
WIPO (PCT)
Prior art keywords
microfluidic device
integration
continuous flow
biochemical protocols
sample
Prior art date
Application number
PCT/IB2000/001137
Other languages
French (fr)
Other versions
WO2001007159A2 (en
Inventor
Yves Fouillet
Claude Vauchier
Jean-Frederic Clerc
Christine Peponnet
Original Assignee
Genset Sa
Commissariat Energie Atomique
Yves Fouillet
Claude Vauchier
Clerc Jean Frederic
Christine Peponnet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9909806A external-priority patent/FR2796863B1/en
Priority claimed from FR9911652A external-priority patent/FR2798604B1/en
Priority claimed from FR9912317A external-priority patent/FR2799139B1/en
Application filed by Genset Sa, Commissariat Energie Atomique, Yves Fouillet, Claude Vauchier, Clerc Jean Frederic, Christine Peponnet filed Critical Genset Sa
Priority to CA002379927A priority Critical patent/CA2379927A1/en
Priority to DE60041264T priority patent/DE60041264D1/en
Priority to AU63109/00A priority patent/AU782726B2/en
Priority to EP00949852A priority patent/EP1203096B1/en
Priority to JP2001512031A priority patent/JP2003505059A/en
Publication of WO2001007159A2 publication Critical patent/WO2001007159A2/en
Publication of WO2001007159A3 publication Critical patent/WO2001007159A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0845—Filaments, strings, fibres, i.e. not hollow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1883—Means for temperature control using thermal insulation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/02—Water baths; Sand baths; Air baths
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F28—HEAT EXCHANGE IN GENERAL
    • F28F—DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION
    • F28F2260/00—Heat exchangers or heat exchange elements having special size, e.g. microstructures
    • F28F2260/02—Heat exchangers or heat exchange elements having special size, e.g. microstructures having microchannels

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Optical Measuring Cells (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided is a microfluidic device comprising a microfluidic substrate comprising at least one pathway for sample flow; and at least one thermal transfer member which is capable of cycling between at least two temperatures. The thermal transfer member is adapted to heat at least a portion of the sample pathway while a sample is flowing along said at least a portion of said sample pathway. Provided also are methods of carrying out biochemical protocols using such a device.
PCT/IB2000/001137 1999-07-28 2000-07-28 Integration of biochemical protocols in a continuous flow microfluidic device WO2001007159A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002379927A CA2379927A1 (en) 1999-07-28 2000-07-28 Integration of biochemical protocols in a continuous flow microfluidic device
DE60041264T DE60041264D1 (en) 1999-07-28 2000-07-28 Flow microreactor act in the local temperature cycles on a flowing sample
AU63109/00A AU782726B2 (en) 1999-07-28 2000-07-28 Integration of biochemical protocols in a continuous flow microfluidic device
EP00949852A EP1203096B1 (en) 1999-07-28 2000-07-28 Continuous flow micro device in which local temperature cycles act on a flowing sample
JP2001512031A JP2003505059A (en) 1999-07-28 2000-07-28 Integration of biochemical protocols in continuous flow microfluidic devices

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
FR9909806A FR2796863B1 (en) 1999-07-28 1999-07-28 PROCESS AND DEVICE FOR CONDUCTING A HEAT TREATMENT PROTOCOL ON A SUBSTANCE IN CONTINUOUS FLOW
FR99/09806 1999-07-28
FR99/11652 1999-09-17
FR9911652A FR2798604B1 (en) 1999-09-17 1999-09-17 DEVICE FOR PERFORMING CHEMICAL OR BIOCHEMICAL REACTIONS BY THERMAL CYCLING
FR99/12317 1999-10-01
FR9912317A FR2799139B1 (en) 1999-10-01 1999-10-01 BIOCHEMICAL ANALYSIS DEVICE COMPRISING A MICROFLUIDIC SUBSTRATE, PARTICULARLY FOR THE AMPLIFICATION OR ANALYSIS OF NUCLEIC ACIDS.

Publications (2)

Publication Number Publication Date
WO2001007159A2 WO2001007159A2 (en) 2001-02-01
WO2001007159A3 true WO2001007159A3 (en) 2001-05-25

Family

ID=27253548

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2000/001137 WO2001007159A2 (en) 1999-07-28 2000-07-28 Integration of biochemical protocols in a continuous flow microfluidic device

Country Status (8)

Country Link
US (2) US7015030B1 (en)
EP (1) EP1203096B1 (en)
JP (1) JP2003505059A (en)
AT (1) ATE419384T1 (en)
AU (1) AU782726B2 (en)
CA (1) CA2379927A1 (en)
DE (1) DE60041264D1 (en)
WO (1) WO2001007159A2 (en)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE43365E1 (en) 2003-03-14 2012-05-08 Lawrence Livermore National Security, Llc Apparatus for chemical amplification based on fluid partitioning in an immiscible liquid
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9126160B2 (en) 2008-09-23 2015-09-08 Bio-Rad Laboratories, Inc. System for forming an array of emulsions
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9194861B2 (en) 2009-09-02 2015-11-24 Bio-Rad Laboratories, Inc. Method of mixing fluids by coalescence of multiple emulsions
US9222128B2 (en) 2011-03-18 2015-12-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US9347059B2 (en) 2011-04-25 2016-05-24 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US9393560B2 (en) 2010-03-25 2016-07-19 Bio-Rad Laboratories, Inc. Droplet transport system for detection
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6977145B2 (en) * 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US8900811B2 (en) 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
FR2822848B1 (en) * 2001-03-28 2004-05-07 Genesystems INTEGRATED AND MINIATURE NUCLEIC ACID SEQUENCING DEVICE
US20030032172A1 (en) * 2001-07-06 2003-02-13 The Regents Of The University Of California Automated nucleic acid assay system
AUPR707101A0 (en) 2001-08-16 2001-09-06 Corbett Research Pty Ltd Continuous flow thermal device
US20030108664A1 (en) * 2001-10-05 2003-06-12 Kodas Toivo T. Methods and compositions for the formation of recessed electrical features on a substrate
US7094379B2 (en) 2001-10-24 2006-08-22 Commissariat A L'energie Atomique Device for parallel and synchronous injection for sequential injection of different reagents
FR2831081B1 (en) * 2001-10-24 2004-09-03 Commissariat Energie Atomique PARALLELISED AND SYNCHRONIZED INJECTION DEVICE FOR SEQUENTIAL INJECTIONS OF DIFFERENT REAGENTS
US20030217923A1 (en) * 2002-05-24 2003-11-27 Harrison D. Jed Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US20040181343A1 (en) * 2002-11-01 2004-09-16 Cellectricon Ab Computer program products and systems for rapidly changing the solution environment around sensors
DE10258398A1 (en) * 2002-12-11 2004-08-12 Hans-Knöll-Institut für Naturstoff-Forschung e.V. DNA amplification method
CA2512071A1 (en) * 2002-12-30 2004-07-22 The Regents Of The University Of California Methods and apparatus for pathogen detection and analysis
JP2004305009A (en) * 2003-04-02 2004-11-04 Hitachi Ltd Apparatus for amplifying nucleic acid and method for amplifying nucleic acid
US8293471B2 (en) * 2004-01-28 2012-10-23 Marshall University Research Corporation Apparatus and method for a continuous rapid thermal cycle system
KR100552706B1 (en) 2004-03-12 2006-02-20 삼성전자주식회사 Method and apparatus for nucleic acid amplification
EP1598429A1 (en) * 2004-05-19 2005-11-23 Amplion Ltd. Detection of amplicon contamination during PCR exhibiting two different annealing temperatures
US7799553B2 (en) * 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
JP4482929B2 (en) * 2004-06-01 2010-06-16 富士フイルム株式会社 Manufacturing method of scientific phenomenon evaluation device
CN102759466A (en) 2004-09-15 2012-10-31 英特基因有限公司 Microfluidic devices
JPWO2006046433A1 (en) * 2004-10-27 2008-05-22 コニカミノルタエムジー株式会社 Microreactor for genetic testing
US7727477B2 (en) * 2004-12-10 2010-06-01 Bio-Rad Laboratories, Inc. Apparatus for priming microfluidics devices with feedback control
CA2611700C (en) * 2005-01-25 2012-08-21 Oscillogy Llc Temperature controller for small fluid samples having different heat capacities
WO2006100812A1 (en) * 2005-03-23 2006-09-28 Fujifilm Corporation Method of experiment with apparatus for evaluating scientific phenomenon and apparatus for evaluating scientific phenomenon
JP4501158B2 (en) * 2005-03-30 2010-07-14 富士フイルム株式会社 Operation method of micro chemical equipment
KR100714988B1 (en) * 2005-08-09 2007-05-09 삼성전자주식회사 Method for DNA purification using bare surface of SiO2 structure,and Purification Apparatus thereby
US7749365B2 (en) 2006-02-01 2010-07-06 IntegenX, Inc. Optimized sample injection structures in microfluidic separations
EP1979079A4 (en) 2006-02-03 2012-11-28 Integenx Inc Microfluidic devices
EP1991357B1 (en) * 2006-02-07 2016-09-14 Stokes Bio Limited A microfluidic analysis system
US7766033B2 (en) * 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US7816121B2 (en) * 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet actuation system and method
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7851184B2 (en) 2006-04-18 2010-12-14 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification method and apparatus
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US10741034B2 (en) 2006-05-19 2020-08-11 Apdn (B.V.I.) Inc. Security system and method of marking an inventory item and/or person in the vicinity
JPWO2007142061A1 (en) * 2006-06-06 2009-10-22 コニカミノルタエムジー株式会社 Microchip inspection device
US7500379B2 (en) * 2006-06-26 2009-03-10 Applied Sensor Research & Development Corporation Acoustic wave array chemical and biological sensor
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
EP1876453A1 (en) * 2006-07-07 2008-01-09 Agilent Technologies, Inc. Integrated chip temperature control
CA2713101C (en) * 2006-10-06 2016-09-13 Vandalia Research, Inc. Method for a continuous rapid thermal cycle system
US8841116B2 (en) * 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
US9114398B2 (en) * 2006-11-29 2015-08-25 Canon U.S. Life Sciences, Inc. Device and method for digital multiplex PCR assays
WO2008115626A2 (en) * 2007-02-05 2008-09-25 Microchip Biotechnologies, Inc. Microfluidic and nanofluidic devices, systems, and applications
EP2465609B1 (en) 2007-06-21 2016-12-28 Gen-Probe Incorporated Method for mixing the contents of a detection chamber
WO2009015296A1 (en) * 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
US8380457B2 (en) * 2007-08-29 2013-02-19 Canon U.S. Life Sciences, Inc. Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes
JP5224801B2 (en) * 2007-12-21 2013-07-03 キヤノン株式会社 Nucleic acid amplification equipment
US20090253181A1 (en) * 2008-01-22 2009-10-08 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
FR2930457B1 (en) * 2008-04-24 2010-06-25 Commissariat Energie Atomique PROCESS FOR MANUFACTURING RECONFIGURABLE MICROCHANNELS
DE102008040062A1 (en) * 2008-07-02 2010-01-07 Robert Bosch Gmbh Method for operating a fluid valve with an oscillating valve movement
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
WO2010077322A1 (en) 2008-12-31 2010-07-08 Microchip Biotechnologies, Inc. Instrument with microfluidic chip
CN105154326A (en) 2009-03-31 2015-12-16 财团法人神奈川科学技术研究院 Liquid reflux high-speed gene amplification device
US8388908B2 (en) 2009-06-02 2013-03-05 Integenx Inc. Fluidic devices with diaphragm valves
WO2010141921A1 (en) 2009-06-05 2010-12-09 Integenx Inc. Universal sample preparation system and use in an integrated analysis system
US20110001963A1 (en) * 2009-07-02 2011-01-06 Durack Gary P System and method for the measurement of multiple emissions from multiple parallel flow channels in a flow cytometry system
CN102472701A (en) * 2009-07-06 2012-05-23 索尼公司 Microfluidic device
WO2011005760A1 (en) * 2009-07-06 2011-01-13 Sony Corporation Microfluidic device having onboard tissue or cell sample handling
US20110003330A1 (en) * 2009-07-06 2011-01-06 Durack Gary P Microfluidic device
US8735088B2 (en) 2009-07-07 2014-05-27 Sony Corporation Method to analyze a sample fluid in a microfluidic cytometry system
CN103331185A (en) * 2009-07-07 2013-10-02 索尼公司 Microfluidic device
WO2011005754A1 (en) * 2009-07-08 2011-01-13 Sony Corporation Microfluidic device having a flow channel within a gain medium
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
WO2012024658A2 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Integrated analysis system
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
JP5889306B2 (en) * 2010-08-23 2016-03-22 ライフ テクノロジーズ コーポレーション Temperature control method for chemical detection system
CN103429331B (en) 2010-11-01 2016-09-28 伯乐生命医学产品有限公司 For forming the system of emulsion
CN103210078B (en) * 2010-11-17 2016-05-11 达雅高生物科技有限公司 Through type detection device
EP2646154A2 (en) * 2010-11-30 2013-10-09 Quantumdx Group Limited The design, fabrication and use of a microfluidics multitemperature flexible reaction device
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
DE102011017596A1 (en) 2011-04-27 2012-10-31 Robert Bosch Gmbh Microfluidic system and method for polymerase chain reaction
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
JP6027321B2 (en) * 2012-03-06 2016-11-16 公益財団法人神奈川科学技術アカデミー High-speed gene amplification detector
JPWO2013141331A1 (en) * 2012-03-22 2015-08-03 和光純薬工業株式会社 Method for detecting DNA having a microsatellite region
US9963740B2 (en) 2013-03-07 2018-05-08 APDN (B.V.I.), Inc. Method and device for marking articles
US9904734B2 (en) 2013-10-07 2018-02-27 Apdn (B.V.I.) Inc. Multimode image and spectral reader
CN110560187B (en) 2013-11-18 2022-01-11 尹特根埃克斯有限公司 Cartridge and instrument for sample analysis
ES2978925T3 (en) 2014-03-18 2024-09-23 Apdn B V I Inc Encrypted optical markers for security applications
US10745825B2 (en) 2014-03-18 2020-08-18 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
US10208332B2 (en) 2014-05-21 2019-02-19 Integenx Inc. Fluidic cartridge with valve mechanism
EP3552690B1 (en) 2014-10-22 2024-09-25 IntegenX Inc. Systems and methods for sample preparation, processing and analysis
US10857536B2 (en) 2016-01-08 2020-12-08 Hewlett-Packard Development Company, L.P. Polymerase chain reaction device
US10519605B2 (en) 2016-04-11 2019-12-31 APDN (B.V.I.), Inc. Method of marking cellulosic products
US10995371B2 (en) 2016-10-13 2021-05-04 Apdn (B.V.I.) Inc. Composition and method of DNA marking elastomeric material
US10920274B2 (en) 2017-02-21 2021-02-16 Apdn (B.V.I.) Inc. Nucleic acid coated submicron particles for authentication
DE102017204705A1 (en) * 2017-03-21 2018-09-27 Robert Bosch Gmbh Mixture of substances and use of a substance mixture for a polymerase chain reaction
US11278894B2 (en) 2017-11-22 2022-03-22 Hewlett-Packard Development Company, L.P. Temperature-controlling microfluidic devices
CN108300656A (en) * 2018-02-05 2018-07-20 冷彦宁 A kind of method of thermal medium flow-type PCR amplification
TWI708842B (en) * 2019-10-02 2020-11-01 中原大學 Bionic organ device and method for making the same

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270183A (en) * 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
EP0636413A2 (en) * 1993-07-28 1995-02-01 The Perkin-Elmer Corporation Nucleic acid amplification reaction apparatus and method
WO1996015269A2 (en) * 1994-11-14 1996-05-23 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
GB2325464A (en) * 1997-04-23 1998-11-25 Bruker Franzen Analytik Gmbh Capillary polymerase chain reaction
US5866345A (en) * 1992-05-01 1999-02-02 The Trustees Of The University Of Pennsylvania Apparatus for the detection of an analyte utilizing mesoscale flow systems
WO1999039005A1 (en) * 1998-01-29 1999-08-05 University Of Pittsburgh Rapid thermocycling for sample analysis
WO1999041015A1 (en) * 1998-02-11 1999-08-19 Institut für Physikalische Hochtechnologie e.V. Miniaturized temperature-zone flow reactor
WO2000023190A1 (en) * 1998-10-16 2000-04-27 Commissariat A L'energie Atomique Device for chemical and/or biological analysis with analysis support

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2565350B1 (en) * 1984-06-05 1986-10-10 Paris Nord Universite PROPER MEANS FOR ALLOWING AUTOMATIC CONTINUOUS SUPPORT, PROCESSING, STORAGE AND ANALYSIS OF BIOLOGICAL SAMPLES
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
WO1993022058A1 (en) 1992-05-01 1993-11-11 Trustees Of The University Of Pennsylvania Polynucleotide amplification analysis using a microfabricated device
US5726026A (en) * 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5580728A (en) 1994-06-17 1996-12-03 Perlin; Mark W. Method and system for genotyping
US5508197A (en) * 1994-07-25 1996-04-16 The Regents, University Of California High-speed thermal cycling system and method of use
DE4435107C1 (en) 1994-09-30 1996-04-04 Biometra Biomedizinische Analy Miniaturized flow thermal cycler
JP3599390B2 (en) 1994-11-04 2004-12-08 日本メクトロン株式会社 Perfluorounsaturated nitrile compound and method for producing the same
US5585069A (en) * 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
AU708076B2 (en) 1995-03-10 1999-07-29 Photocure Asa Esters of 5-aminolevulinic acid as photosensitizing agents in photochemotherapy
US5589136A (en) 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
US5872010A (en) 1995-07-21 1999-02-16 Northeastern University Microscale fluid handling system
US5795976A (en) 1995-08-08 1998-08-18 The Board Of Trustees Of The Leland Stanford Junior University Detection of nucleic acid heteroduplex molecules by denaturing high-performance liquid chromatography and methods for comparative sequencing
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US5716825A (en) 1995-11-01 1998-02-10 Hewlett Packard Company Integrated nucleic acid analysis system for MALDI-TOF MS
CA2236451A1 (en) 1995-11-03 1997-05-09 Zygmunt M. Andrevski Assay system and method for conducting assays
US5736314A (en) 1995-11-16 1998-04-07 Microfab Technologies, Inc. Inline thermo-cycler
US5942443A (en) * 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US6054277A (en) 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US6136212A (en) * 1996-08-12 2000-10-24 The Regents Of The University Of Michigan Polymer-based micromachining for microfluidic devices
US6379929B1 (en) 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
SE9700205D0 (en) 1997-01-24 1997-01-24 Peter Lindberg Integrated microfluidic element
CA2284612A1 (en) 1997-04-04 1998-10-15 Michael Knapp Closed-loop biochemical analyzers
EP1009995A4 (en) 1997-09-02 2007-05-02 Caliper Life Sciences Inc Microfluidic system with electrofluidic and electrothermal controls
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270183A (en) * 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US5866345A (en) * 1992-05-01 1999-02-02 The Trustees Of The University Of Pennsylvania Apparatus for the detection of an analyte utilizing mesoscale flow systems
EP0636413A2 (en) * 1993-07-28 1995-02-01 The Perkin-Elmer Corporation Nucleic acid amplification reaction apparatus and method
WO1996015269A2 (en) * 1994-11-14 1996-05-23 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
GB2325464A (en) * 1997-04-23 1998-11-25 Bruker Franzen Analytik Gmbh Capillary polymerase chain reaction
WO1999039005A1 (en) * 1998-01-29 1999-08-05 University Of Pittsburgh Rapid thermocycling for sample analysis
WO1999041015A1 (en) * 1998-02-11 1999-08-19 Institut für Physikalische Hochtechnologie e.V. Miniaturized temperature-zone flow reactor
WO2000023190A1 (en) * 1998-10-16 2000-04-27 Commissariat A L'energie Atomique Device for chemical and/or biological analysis with analysis support

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KOPP ET AL: "chemical amplification:continuous flow PCR on a chip", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 280, no. 280, 15 May 1998 (1998-05-15), pages 1046 - 1048-1048, XP002107956, ISSN: 0036-8075 *

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE43365E1 (en) 2003-03-14 2012-05-08 Lawrence Livermore National Security, Llc Apparatus for chemical amplification based on fluid partitioning in an immiscible liquid
USRE45539E1 (en) 2003-03-14 2015-06-02 Lawrence Livermore National Security, Llc Method for chemical amplification based on fluid partitioning in an immiscible liquid
USRE46322E1 (en) 2003-03-14 2017-02-28 Lawrence Livermore National Security, Llc Method for chemical amplification based on fluid partitioning in an immiscible liquid
US9126160B2 (en) 2008-09-23 2015-09-08 Bio-Rad Laboratories, Inc. System for forming an array of emulsions
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9216392B2 (en) 2008-09-23 2015-12-22 Bio-Rad Laboratories, Inc. System for forming an array of emulsions
US9243288B2 (en) 2008-09-23 2016-01-26 Bio-Rad Laboratories, Inc. Cartridge with lysis chamber and droplet generator
US9248417B2 (en) 2008-09-23 2016-02-02 Bio-Rad Laboratories, Inc. System for droplet-based assays using an array of emulsions
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9194861B2 (en) 2009-09-02 2015-11-24 Bio-Rad Laboratories, Inc. Method of mixing fluids by coalescence of multiple emulsions
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9393560B2 (en) 2010-03-25 2016-07-19 Bio-Rad Laboratories, Inc. Droplet transport system for detection
US9222128B2 (en) 2011-03-18 2015-12-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US9347059B2 (en) 2011-04-25 2016-05-24 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter

Also Published As

Publication number Publication date
EP1203096A2 (en) 2002-05-08
US7015030B1 (en) 2006-03-21
DE60041264D1 (en) 2009-02-12
AU782726B2 (en) 2005-08-25
JP2003505059A (en) 2003-02-12
AU6310900A (en) 2001-02-13
EP1203096B1 (en) 2008-12-31
WO2001007159A2 (en) 2001-02-01
ATE419384T1 (en) 2009-01-15
CA2379927A1 (en) 2001-02-01
US20060011478A1 (en) 2006-01-19

Similar Documents

Publication Publication Date Title
WO2001007159A3 (en) Integration of biochemical protocols in a continuous flow microfluidic device
WO2002060584A3 (en) Method for carrying out a biochemical protocol in continuous flow in a microreactor
CA2303277A1 (en) Apparatus for a fluid impingement thermal cycler
WO2006025929A3 (en) Thermal device, system, and method, for fluid processing device
WO2003037514A3 (en) Method and apparatus for temperature gradient microfluidics
AU2001271920A1 (en) Chuck with heat exchanger and temperature control fluid
WO2001052323A3 (en) Thermal joint and method of use
WO2004042357A3 (en) Thermal micro-valves for micro-integrated devices
RU2005141456A (en) SYSTEM AND METHOD OF HEATING, COOLING AND THERMAL CYCLING IN A MICRO-LIQUID DEVICE
WO2005082043A3 (en) Thermal cycling device
AU2002219826A1 (en) Liquid cooled heat exchanger with enhanced flow
GB2384624A (en) Thermoelectric control of fluid temperature
AUPR707101A0 (en) Continuous flow thermal device
GB2340217B (en) Multi-mode heat transfer using a thermal heat pipe valve
AU2002222799A1 (en) Heat exchanger
AU2001290590A1 (en) Heat exchanger with bypass seal allowing differential thermal expansion
WO2008010851A3 (en) Heat sink and method of making same
AU2001283578A1 (en) Method and sensor for mass flow measurement using probe heat conduction
WO2003007677A3 (en) Thermal cycling system and method of use
DE69905194D1 (en) Thermozykliergerät
EP1403607A3 (en) Thermal processing roller and temperature control apparatus for roller
AU3022599A (en) Thermoelectric cooling device using heat pipe for conducting and radiating
WO2003006162A3 (en) Thermal cycling methods and apparatus
WO2006039293A3 (en) Localized control of thermal properties on microdevices and applications thereof
WO2001049112A3 (en) Oocyte vitrification technique

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 63109/00

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2379927

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2000949852

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000949852

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWG Wipo information: grant in national office

Ref document number: 63109/00

Country of ref document: AU