WO2001006016A3 - Amplification of nucleic acids with electronic detection - Google Patents

Amplification of nucleic acids with electronic detection Download PDF

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Publication number
WO2001006016A3
WO2001006016A3 PCT/US2000/019889 US0019889W WO0106016A3 WO 2001006016 A3 WO2001006016 A3 WO 2001006016A3 US 0019889 W US0019889 W US 0019889W WO 0106016 A3 WO0106016 A3 WO 0106016A3
Authority
WO
WIPO (PCT)
Prior art keywords
amplification
nucleic acids
electronic detection
detection
etm
Prior art date
Application number
PCT/US2000/019889
Other languages
French (fr)
Other versions
WO2001006016A9 (en
WO2001006016A2 (en
Original Assignee
Clinical Micro Sensors Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clinical Micro Sensors Inc filed Critical Clinical Micro Sensors Inc
Priority to AU63605/00A priority Critical patent/AU6360500A/en
Priority to EP00950511A priority patent/EP1194593A2/en
Priority to CA002379693A priority patent/CA2379693A1/en
Priority to JP2001511224A priority patent/JP2003530822A/en
Publication of WO2001006016A2 publication Critical patent/WO2001006016A2/en
Publication of WO2001006016A3 publication Critical patent/WO2001006016A3/en
Publication of WO2001006016A9 publication Critical patent/WO2001006016A9/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Nanotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Composite Materials (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to compositions and methods useful in the detection of nucleic acids using a variety of amplification techniques, including both signal amplification and target amplification. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the nucleic acid, either directly or indirectly, to allow electronic detection of the ETM using an electrode.
PCT/US2000/019889 1999-07-20 2000-07-20 Amplification of nucleic acids with electronic detection WO2001006016A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU63605/00A AU6360500A (en) 1999-07-20 2000-07-20 Amplification of nucleic acids with electronic detection
EP00950511A EP1194593A2 (en) 1999-07-20 2000-07-20 Amplification of nucleic acids with electronic detection
CA002379693A CA2379693A1 (en) 1999-07-20 2000-07-20 Amplification of nucleic acids with electronic detection
JP2001511224A JP2003530822A (en) 1999-07-20 2000-07-20 Amplification of electronic nucleic acid detection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14469899P 1999-07-20 1999-07-20
US60/144,698 1999-07-20

Publications (3)

Publication Number Publication Date
WO2001006016A2 WO2001006016A2 (en) 2001-01-25
WO2001006016A3 true WO2001006016A3 (en) 2001-08-23
WO2001006016A9 WO2001006016A9 (en) 2002-07-11

Family

ID=22509738

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/019889 WO2001006016A2 (en) 1999-07-20 2000-07-20 Amplification of nucleic acids with electronic detection

Country Status (5)

Country Link
EP (1) EP1194593A2 (en)
JP (1) JP2003530822A (en)
AU (1) AU6360500A (en)
CA (1) CA2379693A1 (en)
WO (1) WO2001006016A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8012743B2 (en) 1998-10-27 2011-09-06 Osmetech Technology Inc. Detection of target analytes using particles and electrodes

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6875619B2 (en) 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
US6518024B2 (en) 1999-12-13 2003-02-11 Motorola, Inc. Electrochemical detection of single base extension
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7341837B2 (en) * 2003-09-02 2008-03-11 Lawton Robert L Soluble analyte detection and amplification
US20070099211A1 (en) * 2005-07-15 2007-05-03 Vissarion Aivazachvili Detection of nucleic acid amplification
EP2118310B1 (en) * 2006-12-29 2013-03-06 Applied Biosystems, LLC Systems and methods for detecting nucleic acids
WO2008083259A1 (en) * 2006-12-29 2008-07-10 Applera Corporation Systems and methods for detecting nucleic acids
JP2009034052A (en) 2007-08-02 2009-02-19 Canon Inc Hybridization method and apparatus
US8084589B2 (en) * 2007-08-31 2011-12-27 University Of Massachusetts Phosphoramidite nucleoside analogs
CA2700945A1 (en) * 2007-09-27 2009-04-02 University Of Western Ontario Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate
US7820391B2 (en) 2007-11-06 2010-10-26 Osmetech Molecular Diagnostics Baseless nucleotide analogues and uses thereof
EP3514244B1 (en) 2009-04-03 2021-07-07 Sequenom, Inc. Nucleic acid preparation methods
WO2019028361A1 (en) * 2017-08-04 2019-02-07 Stratos Genomics Inc. Single molecule nucleic acid detection by mismatch cleavage
US20190062809A1 (en) 2017-08-24 2019-02-28 Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) Electrochemical detection of bacterial and/or fungal infections
JP7353301B2 (en) 2018-05-07 2023-09-29 アルニラム ファーマスーティカルズ インコーポレイテッド Extrahepatic delivery
EP3824099A4 (en) * 2018-12-13 2021-08-11 Hewlett-Packard Development Company, L.P. Multiplex nucleic acid detection
CN114113265B (en) * 2021-12-14 2023-08-08 郑州轻工业大学 Aptamer sensor and preparation method thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986005815A1 (en) * 1985-03-25 1986-10-09 Genetics International Inc. Nucleic acid sequences attached to materials sensitive to magnetic fields, and methods of assay and apparatus using such attached sequences.
WO1996040712A1 (en) * 1995-06-07 1996-12-19 California Institute Of Technology Nucleic acid mediated electron transfer
WO1998020162A2 (en) * 1996-11-05 1998-05-14 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
WO1998057159A1 (en) * 1997-06-12 1998-12-17 Clinical Micro Sensors, Inc. Electronic methods for the detection of analytes
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
WO1999037819A2 (en) * 1998-01-27 1999-07-29 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986005815A1 (en) * 1985-03-25 1986-10-09 Genetics International Inc. Nucleic acid sequences attached to materials sensitive to magnetic fields, and methods of assay and apparatus using such attached sequences.
WO1996040712A1 (en) * 1995-06-07 1996-12-19 California Institute Of Technology Nucleic acid mediated electron transfer
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
WO1998020162A2 (en) * 1996-11-05 1998-05-14 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
WO1998057159A1 (en) * 1997-06-12 1998-12-17 Clinical Micro Sensors, Inc. Electronic methods for the detection of analytes
WO1999037819A2 (en) * 1998-01-27 1999-07-29 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BANER J ET AL: "Signal amplification of padlock probes by rolling circle replication", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 26, no. 22, 15 November 1998 (1998-11-15), pages 5073 - 5078, XP002112357, ISSN: 0305-1048 *
BROWN G ET AL: "Metallorganic labels for DNA sequencing and mapping", NEW JOURNAL OF CHEMISTRY,FR,CNRS-GAUTHIER-VILLARS, MONTROUGE, vol. 18, no. 3, March 1994 (1994-03-01), pages 317 - 326, XP002106965, ISSN: 1144-0546 *
UTO Y ET AL: "Electrochemical analysis of DNA amplified by the polymerase chain reaction with a ferrocenylated oligonucleotide", ANALYTICAL BIOCHEMISTRY,ACADEMIC PRESS, SAN DIEGO, CA,US, vol. 250, 1997, pages 122 - 124, XP002106964, ISSN: 0003-2697 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8012743B2 (en) 1998-10-27 2011-09-06 Osmetech Technology Inc. Detection of target analytes using particles and electrodes

Also Published As

Publication number Publication date
CA2379693A1 (en) 2001-01-25
AU6360500A (en) 2001-02-05
JP2003530822A (en) 2003-10-21
WO2001006016A9 (en) 2002-07-11
EP1194593A2 (en) 2002-04-10
WO2001006016A2 (en) 2001-01-25

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