WO2001006003A3 - Procede et necessaire pour la determination de cassures bicatenaires ou monocatenaires d'adn, et dispositif pour la filtration controlee en particulier de molecules d'adn avec des plaques de filtration a puits - Google Patents

Procede et necessaire pour la determination de cassures bicatenaires ou monocatenaires d'adn, et dispositif pour la filtration controlee en particulier de molecules d'adn avec des plaques de filtration a puits Download PDF

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Publication number
WO2001006003A3
WO2001006003A3 PCT/EP2000/006842 EP0006842W WO0106003A3 WO 2001006003 A3 WO2001006003 A3 WO 2001006003A3 EP 0006842 W EP0006842 W EP 0006842W WO 0106003 A3 WO0106003 A3 WO 0106003A3
Authority
WO
WIPO (PCT)
Prior art keywords
carrying
strand breaks
kit
single strand
filter plates
Prior art date
Application number
PCT/EP2000/006842
Other languages
German (de)
English (en)
Other versions
WO2001006003A2 (fr
Inventor
Werner E G Mueller
Heinz C Schroeder
Renato Batel
Original Assignee
Werner E G Mueller
Heinz C Schroeder
Renato Batel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Werner E G Mueller, Heinz C Schroeder, Renato Batel filed Critical Werner E G Mueller
Priority to AU62751/00A priority Critical patent/AU6275100A/en
Priority to EP00949369A priority patent/EP1194591A2/fr
Publication of WO2001006003A2 publication Critical patent/WO2001006003A2/fr
Publication of WO2001006003A3 publication Critical patent/WO2001006003A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne, entre autres, un procédé de détermination de cassures bicaténaires d'ADN, qui comprend les étapes suivantes: a) dépôt des cellules et/ou des tissus à analyser sur des filtres spécifiques; b) lyse des cellules/tissus avec au moins un détergent, au moins une substance chaotrope et au moins un colorant fluorescent fixant spécifiquement un double brin d'ADN; c) élution des morceaux résultant de la cassure d'ADN, libérés par la lyse, à travers les filtres spécifiques; d) évaluation par spectroscopie à fluorescence. Pour un puits, seulement une fraction d'éluat est collectée.
PCT/EP2000/006842 1999-07-19 2000-07-18 Procede et necessaire pour la determination de cassures bicatenaires ou monocatenaires d'adn, et dispositif pour la filtration controlee en particulier de molecules d'adn avec des plaques de filtration a puits WO2001006003A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU62751/00A AU6275100A (en) 1999-07-19 2000-07-18 Method and kit for determining dna double/single strand breaks and device for carrying out the controlled filtration of, in particular, dna molecules with well filter plates
EP00949369A EP1194591A2 (fr) 1999-07-19 2000-07-18 Procede et necessaire pour la determination de cassures bicatenaires ou monocatenaires d'adn, et dispositif pour la filtration controlee en particulier de molecules d'adn avec des plaques de filtration a puits

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19933078.6 1999-07-19
DE19933078A DE19933078B4 (de) 1999-07-19 1999-07-19 Verfahren und Kit zur Bestimmung von DNA-Doppel-/Einzelstrangbrüchen und Vorrichtung zum kontrollierten Filtrieren von insbesondere DNA-Moleküle und/oder andere Moleküle/Molekülkomplexe enthaltenden flüssigen Medien mit Well-Filterplatten

Publications (2)

Publication Number Publication Date
WO2001006003A2 WO2001006003A2 (fr) 2001-01-25
WO2001006003A3 true WO2001006003A3 (fr) 2001-12-13

Family

ID=7914823

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/006842 WO2001006003A2 (fr) 1999-07-19 2000-07-18 Procede et necessaire pour la determination de cassures bicatenaires ou monocatenaires d'adn, et dispositif pour la filtration controlee en particulier de molecules d'adn avec des plaques de filtration a puits

Country Status (4)

Country Link
EP (1) EP1194591A2 (fr)
AU (1) AU6275100A (fr)
DE (1) DE19933078B4 (fr)
WO (1) WO2001006003A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10111853A1 (de) * 2001-03-05 2002-11-14 Wita Proteomics Ag Verfahren und Vorrichtung zur Aufarbeitung von Proteinen in Gelen
US20030202909A1 (en) * 2002-04-29 2003-10-30 Genetix Limited Vacuum manifold and uses thereof
US7824623B2 (en) 2003-06-24 2010-11-02 Millipore Corporation Multifunctional vacuum manifold
EP1491258B1 (fr) 2003-06-24 2008-12-03 Millipore Corporation Systeme de vide multifonctionnelle

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2618556A1 (fr) * 1987-07-23 1989-01-27 Adria Procede permettant de detecter si un produit alimentaire a ete irradie
WO1997010055A1 (fr) * 1995-09-15 1997-03-20 Beckman Instruments, Inc. Tubulure a vide pour le traitement en laboratoire d'echantillons liquides multiples
DE19724781A1 (de) * 1997-06-12 1998-12-24 Werner E G Prof Dr Mueller Mikro-Methode zur Schnellbestimmung von DNA-Schäden und deren Reparatur unter Verwendung des Fluoreszenzfarbstoffs Picogreen und ihre Anwendung
DE19725894A1 (de) * 1997-06-19 1998-12-24 Biotechnolog Forschung Gmbh Differentielle Vakuumkammer

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2618556A1 (fr) * 1987-07-23 1989-01-27 Adria Procede permettant de detecter si un produit alimentaire a ete irradie
WO1997010055A1 (fr) * 1995-09-15 1997-03-20 Beckman Instruments, Inc. Tubulure a vide pour le traitement en laboratoire d'echantillons liquides multiples
DE19724781A1 (de) * 1997-06-12 1998-12-24 Werner E G Prof Dr Mueller Mikro-Methode zur Schnellbestimmung von DNA-Schäden und deren Reparatur unter Verwendung des Fluoreszenzfarbstoffs Picogreen und ihre Anwendung
DE19725894A1 (de) * 1997-06-19 1998-12-24 Biotechnolog Forschung Gmbh Differentielle Vakuumkammer

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DUCORE J.M.: "EDTA alkaline elution characteristics and measurement of DNA damage in unlabeled DNA using Hoechst 33258 fluorescence", ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, vol. 11, 1988, US, pages 449 - 460, XP000995522 *

Also Published As

Publication number Publication date
DE19933078B4 (de) 2004-07-08
EP1194591A2 (fr) 2002-04-10
DE19933078A1 (de) 2001-02-08
WO2001006003A2 (fr) 2001-01-25
AU6275100A (en) 2001-02-05

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