WO2001005800A3 - A method for the enrichment of heteroduplexes and its use in mutation detection - Google Patents

A method for the enrichment of heteroduplexes and its use in mutation detection Download PDF

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Publication number
WO2001005800A3
WO2001005800A3 PCT/EP2000/006829 EP0006829W WO0105800A3 WO 2001005800 A3 WO2001005800 A3 WO 2001005800A3 EP 0006829 W EP0006829 W EP 0006829W WO 0105800 A3 WO0105800 A3 WO 0105800A3
Authority
WO
WIPO (PCT)
Prior art keywords
heteroduplexes
nucleic acid
protein
selectively
enrichment
Prior art date
Application number
PCT/EP2000/006829
Other languages
French (fr)
Other versions
WO2001005800A2 (en
Inventor
Borries Kemper
Stefan Golz
Original Assignee
Borries Kemper
Stefan Golz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Borries Kemper, Stefan Golz filed Critical Borries Kemper
Priority to AU66948/00A priority Critical patent/AU6694800A/en
Publication of WO2001005800A2 publication Critical patent/WO2001005800A2/en
Publication of WO2001005800A3 publication Critical patent/WO2001005800A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Abstract

A method for selectively enriching heteroduplexes from a mixture of hetero- and homoduplexes comprising binding selectively said heteroduplexes to a heteroduplex binding protein thereby forming a complex between the heteroduplex and the protein. The method is characterized in that the protein is a modified form of a wild-type resolvase, which modified form in comparison to the wild type resolvase retains an ability to selectively bind to heteroduplexes, and has a modified ability to cleave heteroduplexes. In case one of the nucleic strands in the heteroduplexes derives from a sample and the other is a standard nucleic acid strand, the method may be used for detecting mismatches in the sample nucleic acid relative the standard nucleic acid.
PCT/EP2000/006829 1999-07-18 2000-07-18 A method for the enrichment of heteroduplexes and its use in mutation detection WO2001005800A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU66948/00A AU6694800A (en) 1999-07-18 2000-07-18 A method for the enrichment of heteroduplexes and its use in mutation detection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9902741-9 1999-07-18
SE9902741A SE9902741D0 (en) 1999-07-19 1999-07-19 Method for the enrichment of heteroduplexes and its use in mutation detection

Publications (2)

Publication Number Publication Date
WO2001005800A2 WO2001005800A2 (en) 2001-01-25
WO2001005800A3 true WO2001005800A3 (en) 2001-05-10

Family

ID=20416533

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/006829 WO2001005800A2 (en) 1999-07-18 2000-07-18 A method for the enrichment of heteroduplexes and its use in mutation detection

Country Status (3)

Country Link
AU (1) AU6694800A (en)
SE (1) SE9902741D0 (en)
WO (1) WO2001005800A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010514452A (en) 2006-12-29 2010-05-06 アプライド バイオシステムズ インコーポレイテッド Concentration with heteroduplex
US9279146B2 (en) * 2012-12-21 2016-03-08 Roche Molecular Systems, Inc. Compounds and methods for the enrichment of mutated nucleic acid from a mixture
US9873908B2 (en) * 2013-11-27 2018-01-23 Roche Molecular Systems, Inc. Methods for the enrichment of mutated nucleic acid from a mixture

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993020233A1 (en) * 1992-03-27 1993-10-14 University Of Maryland At Baltimore Detection of gene mutations with mismatch repair enzymes
WO1996035809A1 (en) * 1995-05-11 1996-11-14 Avitech Diagnostics Inc Detection of mismatches by resolvase cleavage on a solid support
WO1997009434A1 (en) * 1995-09-08 1997-03-13 University Of Dundee Dna structure-specific nucleases
US5698400A (en) * 1994-04-25 1997-12-16 Avitech Diagnostics, Inc. Detection of mutation by resolvase cleavage
DE19757144A1 (en) * 1997-12-20 1999-06-24 Boerries Prof Dr Rer Na Kemper Detection of genomic mutations

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993020233A1 (en) * 1992-03-27 1993-10-14 University Of Maryland At Baltimore Detection of gene mutations with mismatch repair enzymes
US5698400A (en) * 1994-04-25 1997-12-16 Avitech Diagnostics, Inc. Detection of mutation by resolvase cleavage
WO1996035809A1 (en) * 1995-05-11 1996-11-14 Avitech Diagnostics Inc Detection of mismatches by resolvase cleavage on a solid support
WO1997009434A1 (en) * 1995-09-08 1997-03-13 University Of Dundee Dna structure-specific nucleases
DE19757144A1 (en) * 1997-12-20 1999-06-24 Boerries Prof Dr Rer Na Kemper Detection of genomic mutations

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DEAN M: "RESOLVING DNA MUTATIONS", NATURE GENETICS,US,NEW YORK, NY, vol. 9, 1 February 1995 (1995-02-01), pages 103 - 104, XP000600254, ISSN: 1061-4036 *
GOLZ AND KEMPER: "Enzymatic mutation detection: enrichment of heteroduplexes from hybrid DNA mixtures by cleavage-deficient GST-tagged endonuclease VII", NUCLEIC ACIDS RESEARCH, vol. 27, no. 15, August 1999 (1999-08-01), pages e7, XP002160595 *
GOLZ S ET AL: "Enzymatic mutation detection. Procedure for screening and mapping of mutations by immobilised endonuclease VII.", NUCLEIC ACIDS RESEARCH, (1998 FEB 15) 26 (4) 1132-3., XP002157680 *
GOLZ STEFAN ET AL: "Identification of amino acids of endonuclease VII essential for binding and cleavage of cruciform DNA.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 245, no. 3, 1997, pages 573 - 580, XP000982151, ISSN: 0014-2956 *
RAAIJMAKERS HANS ET AL: "X-ray structure of T4 endonuclease VII: A DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 18, no. 6, 15 March 1999 (1999-03-15), pages 1447 - 1458, XP002160594, ISSN: 0261-4189 *

Also Published As

Publication number Publication date
WO2001005800A2 (en) 2001-01-25
SE9902741D0 (en) 1999-07-19
AU6694800A (en) 2001-02-05

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