WO2000078974A3 - Overexpression in yeast and plants of a gene encoding glycerol 3-phosphate acyltransferase - Google Patents

Overexpression in yeast and plants of a gene encoding glycerol 3-phosphate acyltransferase Download PDF

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Publication number
WO2000078974A3
WO2000078974A3 PCT/CA2000/000738 CA0000738W WO0078974A3 WO 2000078974 A3 WO2000078974 A3 WO 2000078974A3 CA 0000738 W CA0000738 W CA 0000738W WO 0078974 A3 WO0078974 A3 WO 0078974A3
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WO
WIPO (PCT)
Prior art keywords
yeast
overexpression
plants
phosphate acyltransferase
gene encoding
Prior art date
Application number
PCT/CA2000/000738
Other languages
French (fr)
Other versions
WO2000078974A2 (en
Inventor
Ravinder K Jain
Samuel L Mackenzie
Original Assignee
Ca Nat Research Council
Ravinder K Jain
Samuel L Mackenzie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Nat Research Council, Ravinder K Jain, Samuel L Mackenzie filed Critical Ca Nat Research Council
Priority to EP00941822A priority Critical patent/EP1190069A2/en
Priority to AU56665/00A priority patent/AU5666500A/en
Priority to CA002375805A priority patent/CA2375805A1/en
Publication of WO2000078974A2 publication Critical patent/WO2000078974A2/en
Publication of WO2000078974A3 publication Critical patent/WO2000078974A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention provides a method for increasing the triacylglyceride content of an organism, and/or for modifying the fatty acid composition of the triacyllyceride, by expressing in the organism a DNA encoding a protein having glycerol 3-phosphate acyltransferase (GPAT) activity. Preferred examples of the method of the invention are illustrated with Arabidopsis thaliana and yeast, using constructs prepared from the plastidial GPAT genes from Safflower and E.Coli.
PCT/CA2000/000738 1999-06-21 2000-06-20 Overexpression in yeast and plants of a gene encoding glycerol 3-phosphate acyltransferase WO2000078974A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00941822A EP1190069A2 (en) 1999-06-21 2000-06-20 Overexpression in yeast and plants of a gene encoding glycerol 3-phosphate acyltransferase
AU56665/00A AU5666500A (en) 1999-06-21 2000-06-20 Overexpression in yeast and plants of a gene encoding glycerol 3-phosphate acyltransferase
CA002375805A CA2375805A1 (en) 1999-06-21 2000-06-20 Overexpression in yeast and plants of a gene encoding glycerol 3-phosphate acyltransferase

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US13978899P 1999-06-21 1999-06-21
US60/139,788 1999-06-21
US18290500P 2000-02-16 2000-02-16
US60/182,905 2000-02-16

Publications (2)

Publication Number Publication Date
WO2000078974A2 WO2000078974A2 (en) 2000-12-28
WO2000078974A3 true WO2000078974A3 (en) 2001-07-05

Family

ID=26837539

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2000/000738 WO2000078974A2 (en) 1999-06-21 2000-06-20 Overexpression in yeast and plants of a gene encoding glycerol 3-phosphate acyltransferase

Country Status (4)

Country Link
EP (1) EP1190069A2 (en)
AU (1) AU5666500A (en)
CA (1) CA2375805A1 (en)
WO (1) WO2000078974A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7033790B2 (en) 2001-04-03 2006-04-25 Curagen Corporation Proteins and nucleic acids encoding same
US7192762B2 (en) 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
JP5890687B2 (en) * 2010-02-03 2016-03-22 サントリーホールディングス株式会社 Glycerol-3-phosphate acyltransferase homolog and use thereof
CN111394399B (en) * 2019-01-03 2022-06-28 上海凯赛生物技术股份有限公司 Method for reducing content of acylglycerol ester impurities in long-chain dibasic acid
CN112980876B (en) * 2021-03-12 2023-01-24 中国农业科学院棉花研究所 Application of GhGPAT12 protein and GhGPAT25 protein in regulation and control of cotton male reproductive development

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992013082A1 (en) * 1991-01-16 1992-08-06 Kirin Beer Kabushiki Kaisha Chilling resistant plants and their production
US5210189A (en) * 1990-01-12 1993-05-11 Norio Murata DNA sequence encoding glycerol 3-phosphate acyltransferase
WO1995014094A1 (en) * 1993-11-19 1995-05-26 Kirin Beer Kabushiki Kaisha Dna chain coding for glycero-3-phosphate acyltransferase and use thereof
WO1996038573A1 (en) * 1995-05-31 1996-12-05 National Research Council Of Canada Modification of plant lipids and seed oils utilizing yeast slc genes
EP0843007A1 (en) * 1995-07-27 1998-05-20 Kirin Beer Kabushiki Kaisha Dna strands encoding glycerol-3-phosphate acyltransferase

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210189A (en) * 1990-01-12 1993-05-11 Norio Murata DNA sequence encoding glycerol 3-phosphate acyltransferase
WO1992013082A1 (en) * 1991-01-16 1992-08-06 Kirin Beer Kabushiki Kaisha Chilling resistant plants and their production
WO1995014094A1 (en) * 1993-11-19 1995-05-26 Kirin Beer Kabushiki Kaisha Dna chain coding for glycero-3-phosphate acyltransferase and use thereof
WO1996038573A1 (en) * 1995-05-31 1996-12-05 National Research Council Of Canada Modification of plant lipids and seed oils utilizing yeast slc genes
EP0843007A1 (en) * 1995-07-27 1998-05-20 Kirin Beer Kabushiki Kaisha Dna strands encoding glycerol-3-phosphate acyltransferase

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BHELLA RESHAM S ET AL: "Nucleotide Sequence of a cDNA from Carthamus tinctorius Encoding a Glycerol-3-Phosphate Acyl Transferase.", PLANT PHYSIOLOGY (ROCKVILLE), vol. 106, no. 4, 1994, pages 1713 - 1714, XP002154601, ISSN: 0032-0889 *
DATABASE EMBL 13 June 1995 (1995-06-13), LIGHTNER, V.A., ET AL.: "E.coli plsB and dgk genes coding for sn-glycerol-3-phosphate acyltransferase and diglyceride kinase.", XP002154604 *
DATABASE EMBL 8 October 1994 (1994-10-08), BHELLA R.S., ET AL.: "Carthamus tinctorius glycerol-3-phosphate acyltransferase mRNA, complete cds.", XP002154603 *
FRENTZEN M (REPRINT): "Acyltransferases from basic science to modified seed oils", FETT - LIPID,WILEY-VCH VERLAG,WEINHEIM,DE, vol. 100, no. 4/05, May 1998 (1998-05-01), pages 161 - 166, XP002122744, ISSN: 0931-5985 *
LIGHTNER, V.A., ET AL.: "The DNA sequences encoding plsB and dgk loci of Escherichia coli", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 258, 1983, pages 10856 - 10861, XP002154602 *
WEIER DAGMAR ET AL: "sn-1-Acylglycerol-3-phosphate acyltransferase of Escherichia coli causes insertion of cis-11 eicosenoic acid into the sn-2 position of transgenic rapeseed oil.", MOLECULAR BREEDING, vol. 4, no. 1, February 1998 (1998-02-01), pages 39 - 46, XP002154617, ISSN: 1380-3743 *
WOLTER FRANK P ET AL: "Chilling sensitivity of Arabidopsis thaliana with genetically engineered membrane lipids.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 11, no. 13, 1992, pages 4685 - 4692, XP002154600, ISSN: 0261-4189 *

Also Published As

Publication number Publication date
EP1190069A2 (en) 2002-03-27
WO2000078974A2 (en) 2000-12-28
CA2375805A1 (en) 2000-12-28
AU5666500A (en) 2001-01-09

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