WO2000077181A3 - Dna manipulation methods, applications for synthetic enzymes and use for polyketide production - Google Patents

Dna manipulation methods, applications for synthetic enzymes and use for polyketide production Download PDF

Info

Publication number
WO2000077181A3
WO2000077181A3 PCT/GB2000/002286 GB0002286W WO0077181A3 WO 2000077181 A3 WO2000077181 A3 WO 2000077181A3 GB 0002286 W GB0002286 W GB 0002286W WO 0077181 A3 WO0077181 A3 WO 0077181A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
restriction
unit
site
recognition site
Prior art date
Application number
PCT/GB2000/002286
Other languages
French (fr)
Other versions
WO2000077181A2 (en
Inventor
Anand Ranganathan
Original Assignee
Qxyz Ltd
Anand Ranganathan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qxyz Ltd, Anand Ranganathan filed Critical Qxyz Ltd
Priority to CA002376559A priority Critical patent/CA2376559A1/en
Priority to EP00940533A priority patent/EP1190045A2/en
Priority to AU55457/00A priority patent/AU5545700A/en
Publication of WO2000077181A2 publication Critical patent/WO2000077181A2/en
Publication of WO2000077181A3 publication Critical patent/WO2000077181A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/08—Oxygen as only ring hetero atoms containing a hetero ring of at least seven ring members, e.g. zearalenone, macrolide aglycons

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention comprises a method of assembling several DNA units in sequence in a DNA construct and all derivatives of this method. In particular the production of synthetic enzymes is contemplated. Each DNA unit is provided with the same restriction enzyme recognition site at its 5' and 3' ends. The restriction recognition site at its 3' end being combined with a recognition site for a DNA modification enzyme. A DNA construct having the same or a compatible accessible restriction site, as provided in the DNA unit, is cleaved at the restriction site by the appropriate restriction enzyme. The desired DNA unit is then inserted into the DNA construct, this ligated product subsequently being brought into contact with a DNA modification enzyme such that the restriction site at the 3' end of the inserted DNA unit is abolished. The ligated product is then cleaved at the remaining unmodified restriction recognition site and a subsequent DNA unit is inserted. This process is repeated introducing each desired DNA unit to give a DNA construct containing all the desired units in sequence.
PCT/GB2000/002286 1999-06-11 2000-06-12 Dna manipulation methods, applications for synthetic enzymes and use for polyketide production WO2000077181A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002376559A CA2376559A1 (en) 1999-06-11 2000-06-12 Dna manipulation methods, applications for synthetic enzymes and use for polyketide production
EP00940533A EP1190045A2 (en) 1999-06-11 2000-06-12 Dna manipulation methods , applications for synthetic enzymes and use for polyketide production
AU55457/00A AU5545700A (en) 1999-06-11 2000-06-12 Dna manipulation methods and applications for synthetic enzymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9913694.7A GB9913694D0 (en) 1999-06-11 1999-06-11 DNA manipulation methods and applications for synthetic enzymes
GB9913694.7 1999-06-11

Publications (2)

Publication Number Publication Date
WO2000077181A2 WO2000077181A2 (en) 2000-12-21
WO2000077181A3 true WO2000077181A3 (en) 2001-05-10

Family

ID=10855226

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/002286 WO2000077181A2 (en) 1999-06-11 2000-06-12 Dna manipulation methods, applications for synthetic enzymes and use for polyketide production

Country Status (5)

Country Link
EP (1) EP1190045A2 (en)
AU (1) AU5545700A (en)
CA (1) CA2376559A1 (en)
GB (1) GB9913694D0 (en)
WO (1) WO2000077181A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10021267A1 (en) * 2000-04-26 2002-01-24 Actinodrug Pharmaceuticals Gmb Process for the production of modular enzyme systems by synthesis of their genes in cyclically repeatable synthesis steps
US8999679B2 (en) 2008-12-18 2015-04-07 Iti Scotland Limited Method for assembly of polynucleic acid sequences
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
WO2020203496A1 (en) * 2019-04-01 2020-10-08 国立大学法人神戸大学 Method for constructing chimeric plasmid library

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4713337A (en) * 1985-01-03 1987-12-15 Massachusetts Institute Of Technology Method for deletion of a gene from a bacteria
US4963487A (en) * 1985-01-03 1990-10-16 Massachusetts Institute Of Technology Method for deletion of a gene from a bacteria
WO1996040968A1 (en) * 1995-06-07 1996-12-19 The Leland Stanford Junior University Recombinant production of novel polyketides
WO1997028282A1 (en) * 1996-01-29 1997-08-07 Stratagene Improved primer-mediated polynucleotide synthesis and manipulation techniques
WO1998017811A1 (en) * 1996-10-24 1998-04-30 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
EP0841402A2 (en) * 1996-09-26 1998-05-13 National Institute Of Agrobiological Resources, Ministry Of Agriculture, Forestry And Fisheries High capacity binary shuttle vector
WO1998038326A1 (en) * 1997-02-28 1998-09-03 Nature Technology Corporation Self-assembling genes, vectors and uses thereof
WO1998049315A2 (en) * 1997-04-30 1998-11-05 Kosan Biosciences, Inc. Combinatorial polyketide libraries produced using a modular pks gene cluster as scaffold
US5863730A (en) * 1995-09-15 1999-01-26 Centre National De La Recherche Scientifique - Cnrs Procedure for the polymerization of nucleic acid sequences and its applications
WO2000063360A1 (en) * 1999-04-16 2000-10-26 Celltech Therapeutics Limited Combinatorial method for producing nucleic acids

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4713337A (en) * 1985-01-03 1987-12-15 Massachusetts Institute Of Technology Method for deletion of a gene from a bacteria
US4963487A (en) * 1985-01-03 1990-10-16 Massachusetts Institute Of Technology Method for deletion of a gene from a bacteria
WO1996040968A1 (en) * 1995-06-07 1996-12-19 The Leland Stanford Junior University Recombinant production of novel polyketides
US5863730A (en) * 1995-09-15 1999-01-26 Centre National De La Recherche Scientifique - Cnrs Procedure for the polymerization of nucleic acid sequences and its applications
WO1997028282A1 (en) * 1996-01-29 1997-08-07 Stratagene Improved primer-mediated polynucleotide synthesis and manipulation techniques
EP0841402A2 (en) * 1996-09-26 1998-05-13 National Institute Of Agrobiological Resources, Ministry Of Agriculture, Forestry And Fisheries High capacity binary shuttle vector
WO1998017811A1 (en) * 1996-10-24 1998-04-30 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
WO1998038326A1 (en) * 1997-02-28 1998-09-03 Nature Technology Corporation Self-assembling genes, vectors and uses thereof
WO1998049315A2 (en) * 1997-04-30 1998-11-05 Kosan Biosciences, Inc. Combinatorial polyketide libraries produced using a modular pks gene cluster as scaffold
WO2000063360A1 (en) * 1999-04-16 2000-10-26 Celltech Therapeutics Limited Combinatorial method for producing nucleic acids

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
MCDANIEL R ET AL: "Multiple genetic modifications of the erythromycin polyketide synthase to produce a library of novel unnatural natural products", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,NATIONAL ACADEMY OF SCIENCE. WASHINGTON,US, vol. 96, no. 5, March 1999 (1999-03-01), pages 1846 - 1851, XP002143433, ISSN: 0027-8424 *
MUTH G ET AL: "Mutational analysis of Streptomyces lividans recA gene suggests that only mutants with residual activity remain viable.", MOLECULAR & GENERAL GENETICS, vol. 255, no. 4, 1997, pages 420 - 428, XP002160032, ISSN: 0026-8925 *
NERENBERG J B ET AL: "TOTAL SYNTHESIS OF THE IMMUNOSUPPRESSIVE AGENT (-)-DISCODERMOLIDE", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,US,AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, vol. 115, no. 26, 1993, pages 12621 - 12622, XP000652058, ISSN: 0002-7863 *
RANGANATHAN ANAND ET AL: "Knowledge-based design of bimodular and trimodular polyketide synthases based on domain and module swaps: A route to simple statin analogues.", CHEMISTRY & BIOLOGY (LONDON), vol. 6, no. 10, October 1999 (1999-10-01), pages 731 - 741, XP000971117, ISSN: 1074-5521 *
ROWE C J ET AL: "Construction of new vectors for high-level expression in actinomycetes", GENE,NL,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, vol. 216, no. 1, August 1998 (1998-08-01), pages 215 - 223, XP004149299, ISSN: 0378-1119 *
TAPIOLAS D M ET AL: "OCTALACTINS A AND B CYTOTOXIC EIGHT-MEMBERED-RING LACTONES FROM A MARINE BACTERIUM STREPTOMYCES-SP", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 113, no. 12, 1991, pages 4682 - 4683, XP002154630, ISSN: 0002-7863 *
TER HAAR ERNST ET AL: "Discodermolide, a cytotoxic marine agent that stabilizes microtubules more potently than taxol.", BIOCHEMISTRY, vol. 35, no. 1, 1996, pages 243 - 250, XP002154629, ISSN: 0006-2960 *
YAMADA SHINYA ET AL: "Total synthesis of (-)-decarestrictine J.", BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 59, no. 9, 1995, pages 1657 - 1660, XP002154631, ISSN: 0916-8451 *

Also Published As

Publication number Publication date
WO2000077181A2 (en) 2000-12-21
EP1190045A2 (en) 2002-03-27
AU5545700A (en) 2001-01-02
CA2376559A1 (en) 2000-12-21
GB9913694D0 (en) 1999-08-11

Similar Documents

Publication Publication Date Title
EP1700915A3 (en) Enzymatic DNA molecules
WO1998005673A8 (en) Methods for purifying nucleic acids
WO1994025477A3 (en) Novel peptide nucleic acids
CA2153898A1 (en) Method for generating single-stranded dna molecules
WO2003014347A3 (en) Plant seed specific promoters
AU1884392A (en) Peptide nucleic acids
EP1741780A3 (en) High affinity oligonucleotide ligands to growth factors
AU2002352108A8 (en) Nucleic acid linkers and use thereof in gene synthesis
WO2002026789A3 (en) Nucleotide sequences mediating male fertility and method of using same
EP2275546A3 (en) Enzymatic DNA molecules
EP1808180A3 (en) Modified GP 100 and uses thereof
WO2002014485A3 (en) Kallikrein gene
AU2001244609A1 (en) Novel protein, dna thereof and process for producing the same
EP1983052A3 (en) Methods of cloning and producing fragment chains with readable information content
WO2002063021A3 (en) Nucleotide sequence mediating male fertility and method of using same
AU4335496A (en) Lactic bacteria producing exopolysaccharides
WO2000077181A3 (en) Dna manipulation methods, applications for synthetic enzymes and use for polyketide production
AU2001260077A1 (en) Microfiltration using activated carbon
EP0896057A3 (en) D-aminoacylase
CA2316113A1 (en) Method for nucleic acid purification using iodine
WO2002079495A3 (en) Genomics applications for modified oligonucleotides
CA2167520A1 (en) Genes encoding branched-chain alpha-ketoacid dehydrogenase complex from streptomyces avermitilis
WO2003040180A3 (en) Genes of corynebacterium glutamicum encoding for genetic stability, gene expression and folding proteins
WO2002029019A3 (en) 2.5-diketo-d-gluconic acid reductases and methods of use
WO2001085905A3 (en) Isolated gum operon from xyllela fastidiosa, isolated nucleic acid molecules therefrom, and uses thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

ENP Entry into the national phase

Ref document number: 2376559

Country of ref document: CA

Ref document number: 2376559

Country of ref document: CA

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: IN/PCT/2001/01144/DE

Country of ref document: IN

WWE Wipo information: entry into national phase

Ref document number: 2000940533

Country of ref document: EP

712F Gb: determination of foreign entitlement (section 12(1)/1977)
713D Gb: proceedings under sect. 13(1) pat. act 1977 ** application filed
WWP Wipo information: published in national office

Ref document number: 2000940533

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: JP

DPE2 Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101)